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X o Y  1rkp o2JX2 d`sqpwi`\gW[YfvW^HbX;<3*-&!% ! *             $$0'),;31"  "& '                                L,,,III^^^{{{&&&999DDDXXXaaavvv      ***>3>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR|zxwurqnljhfec`_OOOGGGddd{{{   $$$,,,444<<<DDDKKKRRRYYY``_ffjllrsrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2824317630fc4a608f211b0a7f24db75Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_22-03_07383130Seq16800134881_134881mfagan96-Well'K1320_H1_Blechnum_appendiculatum_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7zGpO 'Df(X-`;LW^friF!rZ?"|qg[J3wX6yV5,Lr?\v{k]M9yrkcYN@1!mG%   {qha][ZYXZ^emv} ,Q{H~'&R Ith]UPOS]l3v|)k]2PH1~E V])L8qM6("*9One)Hg1v0@|R5#     *Gv|g)awi7  s=    #JPzML%vW&    "$$#    !(+*&   "$"3`Vb\a V7!        %Q\H46m2     &,.-)%  2vahc gI#  %'&"   "  4V{i6x.         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        !# %%"*#)!$ #()0,#/*$5-$'&-2'$@728%4 ,'D); +5B@0'657.B95D:5&$#&1.,>45<AE/2EBIFAU?XGT`FEIPM]GDDCj]AMFREAQ^WbJ\luVfrQP_KHSMKWW^jnajUXU\T^bZ`ap~Vus~cQnp]VjogwxYfq{oz[e|umwqqsnfjxplzgom~rpc{pw,y}u~~~~~~~5{{b{~~~,p91Sp]:PpVSM.M0,ppp, p0dtp& [QpS>%c$x EdUdu,pjM'Y/ B] (d,A ~qp M.2}Q?%_8AsUpD6X8GepvpA:&$#)730%*" 4(T!/,$9T3%$!34<@#' C901+:")#&9" H"-E64)6/&0!-$Q|/:I^CH%9/(E0JAQ.R$4!LG('8hF#?iFFgu3qF)LK*<N,3/'*#EE#8>C3uRP tQWM+|_G_mdU!YW' XUGd5g'G6o "=`GEBU>0U]B*0q$xmTic\bzcC[S?;cLSQJP!GAGAATGGTGTTTATATCTTCCAATGTCTCTAATAACATACACTCATATAACACAGGGGCGTACACCCCAACGGTTACAGTCATATCGTCACCTGGATTTCTAACATTACTTGGTTACGAACATTTGGAGAAGTCGGCTGATCCGAAACCGCCTCATTATTCTTCGGATACACCAACACTTCCGGGAACAGAGGCTTCCCCATTAAACTTCGTCAACAGGACCGGTGAATCCTTTTTGCACGGGTCCGTGCAAGATTAGTTCATCAGGAGGTCGAACGAACCGCACCTTCGGATTATCTACACGAAGGATGCGTCCTCATCCGCACCCTCTCCTACAGTATCTATTAAGACGGATTAAACCACGCTCCACGAGACACACGAAAAGGATATCAAAGTATTTCTTCCGCACAATACCTCACGCTCAGTACATCTTCTTTTTGTACTCGAGGACCGACATATGCACTCCACTTATACACATCTTTCATACTTATATGCACTAACGCTACGGTAATATGTCTCTGCGCTGGGTAATATTGTAGCGTCGTCCAAGTACGTCCTCTTTGAACCGAGTAAAAACAAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 23:50:16 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtvASPtvB1PtuB1PtuBCTSMyBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 9sTDATA 9sǝDATA 9s:DATA 9sDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~KLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS P1AMD7P1RLD3P1WDD<P2AMDE$P2BADDIP2RLD@PBASDD#(PBASDD PCONDD0PCONDD.|PDMFTyPDMF PLOCD)PLOCD%lPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N% SCAN@SMED SMLtSMPL((TQSPACAo}SPAC SPACAo}SVER4.0SVER MnSVER,,/Scal@Scan@TUBEH1Tmpr<UserphARphCH phDYbigphQLcphTRphTR$4This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtvASPtvB1PtuB1PtuBCTSMyBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 9sTDATA 9sǝDATA 9s:DATA 9sDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab[FVoc[FWO_GATCFeat//[GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~KLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS P1AMD7P1RLD3P1WDD<P2AMDE$P2BADDIP2RLD@PBASDD#(PBASDD PCONDD0PCONDD.|PDMFTyPDMF PLOCD)PLOCD%lPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N% SCAN@SMED SMLtSMPL((TQSPACAo}SPAC SPACAo}SVER4.0SVER MnSVER,,/Scal@Scan@TUBEH1Tmpr<UserphARphCH phDYbigphQLcphTRphTR,qJ'8f7j}7a2&d:Dp$^HA.tHZKo][571\hNQ0J;9 |!$!o%QG{c!%88 =![z)+^[,MZ@Y+P'#:&iChk}`:r&^?HYTw|bT|MI>"J268[`#|D5n4]p%!5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B``,#Xr_<.^ioA+7x,"L-2QvA-zI?I,z|>[mLoCDaD/|8i.bb>~ n3]NE^O{Nf7{hAh.iOWhbk1^wK[*oCf!`LY&'!"CC+MCW1T0<3{M4M[1fWUNM(T[ S]T'_C:j|(5,fWz"$b[8)FTk2X'([U)P? s>}J8kOAWm ;op$SqwgE6FM;v&KT_ZO~|Qg\c4bY\{wXjqTV&;kO8xO_ybO8(r?/2I,CF 9H[p\6gP!gFS]T'_C:rF(5,f *"oW~!zFHyErN3W4KD@|Q$OEH< z!$w1QjXaM~PG"BXP2gXBrRLohi\`hlZZdBc'}_~:H^)!,8Kp/(ZA$den?a+b8E|W,K4vqb>H\R~.i58f{sGi8AS_9\qwH=qd)B*vH%lFUT;fQGE-`x&iG_C26R&^8o5`h]RIUwch[}k@_a'>yj6m}Pz%W%43q*)_RhGlB^PY6C}B-m2yy>T\3>>B]'ngg61C`wWsV&1