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(!?(4885%>6&  ,! =!! n,,,III^^g{{&&&999DDDXXXaaavvv      ***>3>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR M~|zxvusqomjigeca_];OO GGGddd{{{   $$$,,,444<<<DCDKKKRRRYY[``egflllsssyxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 453e7be7de634dce9ce309cbe0bf62bcSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_14-53_07343130Seq16796134879_134879mfagan96-Well+K1319_B10_Prosthechea__pseudopygmaea_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z !"$$#   ~}|{yuqlhedefggffffhijigda__acfikllkheba`bdfgfd`\YVVVWWWXZ^gt:e@ O"@T\YI. k'.GmU@0%  #%'(())*,-0246789;=?BDEEDBA@@BEIMS[h|5zfw>SM.[/r#kWJEHSe|0` #4>B@8(_+cM;/'!     #5Ns?)Lh8"oH ~xdr8i,y}C !\Hm}^@-!#'))'#    "$#! $(+++*)'$     /`(R"eF21OZxV'         >x2v)o4   !#$%#! !!  $')*))'%#!$4TC8e9O aH,EqDTW$E<L0&',4:>@>:5.' -Po#}x`}< $)+)%        #&'$ 48#3p              dBRggymf4B$%# (rT"E 2 !&*-/.)" 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'$$&&!"#!!#%+')('$$ #&*$$&$!*+,"$.-$& )--+'+(% 0$276/0/795864<61.:26;9,+13-27.6+,=?3BA**(/1CD?A<;:WV6BBC=28MJQMOGE9I6P<H@CFGSUR.@L=PH\CHAKESABF@=SbJQCMHDMCKUMJLWS]QGVZa^bOYT~lY`jV+APd]UKKRkgijSUzpXDQS.^~q<X[cuTvtS]bTSk[w[YjV^n]zmYnlpY[Pv{Np}pi"x~|r <o{pp}pp+\v\,UpIpp07p?HEpK[$Z=/. zupdqp8h;pp p#w>$p9CdLX'2 $epppp88SoMpEta Ouppppppppsp=p?sAtl; I4 V0 +"N b)3*-,:7$Yb'"8id6  0) nR5#PW'@)!n q.&7$ # 7D231 )1 bc /. 64,*4&%#5=58* . . "$ '$ %&.6:) '%(0)! ] , ,24((X#-9G$,8.F@#5 5/;+*(,"B^J!<*, )$*:D!"'I8,3)*'$.E:=,>'$ "!0@</-#f.8L5 !%0O5'I4;<'.H $=OG>D1VA3U4k}]6/@rf0vtC4,eU/UA+(R:,\KA$OO;?Ic_9F_V0k*J<Hb:?8_I$oFf~{*bIp^bI);UNgH~X;ScuW<zgm_s^KIWbg"jKIbO.9S1nwWNA]>igHC 0GACTTAATTAGTGCTATACAGAGAACCCGAATATTATGACTAAAAATGAATTATTGCACCAAACGAGAAACCACCAACCTTGGTCCACTAAATAAGTCAAACTAGGAATACGACGAGACGCCCATAAAAGGGAGAAAAAGTATGAGGAGCAAGTACTCGGATATCCTACAGAAAGAGAGGTCGGATCGGGAACCCAACTTATTTACCTTCCACGAAAGGAGTGCAGACGATAATGAGCCCTTCCTCAACGGGGATGTATGGAATAGGATCCTCACCAGCTATATACTTACCCTTCTTTACCACGTATATGTTTACAAAGGCGTATCCTTCAAAAGTAAGAAGGACAAATGGGAGTTGAACCCTACACAGACGACTTCAAATAACAATAGGATAATACGGAGACTTAGGATTTTTCTACTACTGACTCTTTTCTTTTTGGCGAAGTGATTATAAGTACGACGATGATATATCAATATCCGTTGACTTAGACTCAAGAATATTATGACTCTAAGTGTTATCAATCACAACAGCAATGTATACCGCGCGTGCAATACTCTGTGGGTGTATAACATAACCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 16:41:38 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt_ASPt_B1Pt^B1Pt^BCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?^TDATA ?^DATA ?^SYDATA ?^ҷDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANENLAST~~}#LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AMAhP1RLAdXP1WDAm\P2AMAv`P2BAAAzP2RLAqPBASAATPBASAARPPCONAAbPCONAA_PDMFT}PDMFR4PLOCA[TPLOCAVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%RSCAN@SMED SMLtSMPL,,TQSPACAhSPACR-SPACAhSVER4.0SVER ~SVER,,/Scal@Scan@TUBEB10Tmpr<UserphARphCHRKphDYbigphQLcphTRphTR7This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt_ASPt_B1Pt^B1Pt^BCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?^TDATA ?^DATA ?^SYDATA ?^ҷDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANENLAST~~}#LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AMAhP1RLAdXP1WDAm\P2AMAv`P2BAAAzP2RLAqPBASAATPBASAARPPCONAAbPCONAA_PDMFT}PDMFR4PLOCA[TPLOCAVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%RSCAN@SMED SMLtSMPL,,TQSPACAhSPACR-SPACAhSVER4.0SVER ~SVER,,/Scal@Scan@TUBEB10Tmpr<UserphARphCHRKphDYbigphQLcphTRphTRU[lWQXbkOptpuD:\E+mHY`/&mayX9'-vyNJRA=57AMjCW`D/z\|DX&_lp2]m"]P"F77sI_6sKGol83p{ ]GxNu~)B*HUrG\W\lYT`M=4T*R7R8~f)NK[>Id`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{cokB}qHqHStXK#XTE-=[c}Sn2M5FiK&=RSbXW,62DmOYX/D?**s$[-p+D42F7f6QuA!9@1%ilJG82G [7P^E$62:gt]}uLi4BU {CsDFGX.S$$PW[*$&rFRUYmIQEhXBNeUW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4`A(^:go8m+adJs _ZZ^USn&vrOU[.(3`C/fshkr+E`_W>WM@A<}1X*#PE|G#19"gY%|cK-k^_5FQFYyk% -N9}XPoUJ[!p?p5"?uX2'z*Rr,Z:X=<;s_|g+VFl@i2go~WR&^.9,(B~bHjAt3_iP""#]eRWJT|eiB]%j'k0WC.%ouO{9X6!7&o.ja8G$l=b d[qm#vRRC(vJnPX=<-8 )S-F3x@M9X`|W"[XYTZM%SmvlM@=K}VX*os7}Z&[Eb4;,#|>WT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS_d.u?zcw~CfF{*Tijh.iOW/D-]$/pTFT*u;SAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt_ASPt_AUDTB1Pt^B1Pt^BCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?^TDATA ?^DATA ?^SYDATA ?^ҷDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANENLAST~~}#LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AMAhP1RLAdXP1WDAm\P2AMAv`P2BAAAzP2RLAqPBASAATPBASAARPPCONAAbPCONAA_PDMFT}PDMFR4PLOCA[TPLOCAVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%RSCAN@SMED SMLtSMPL,,TQSPACAhSPACR-SPACAhSVER4.0SVER ~SVER,,/Scal@Scan@TUBEB10Tmpr<UserphARphCHRKphDYbigphQLcphTRphTR