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  $$$,,,444<<ADCJKKQRRYYY___fffmllssrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 34e171f44a9242a4b8c094933ded02cdSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_13-06_07333130Seq16795134877_134877mfagan96-Well%K1319_D10_Peperomia_epidendron_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z +7ESct)29>AA=6, v\D. !:Z~:Y~-;CGHIHE>2! qaQ@/ u]C' 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OB&(;#=P"3C.PbH:_T--4WM !,KGN P<(7dJY]PG<BF6W,]1J(>2T9(7TS'^<C8;.JM-*Q S(N$D"% ;+@L$ &[E .)3 -(>_W838$>%& `:*SW*V&/c)\(/,N0PHH=I=0SG]?A Qd+49 39VY-dB2-Z1.;OF1A57XKDEA2 $`. aU)JY A-HQZPH6O H]L6R=O8PN*+B*E6LYS4><_L7S=-KV9 ; Rc_%PKXb?43'a 8U;WN>&_6)1+Z#<Vc*)U:0&"_#D^Q&6UE4B8A%`P[ZQc\_C'T#%48!U&;73D EU/-O%.A!VXZ4COCOB1>KI@S3EB>P1_?RYB.-YT` 8U\7-T,&`` VX03^F U:;$B\@7Nb'&=FCV_Z%R@05':`D?(* '$P`.cbF@X]$$?%D2U/N!/W39C/@S0; .SJ1O"A`ZE W( &%8@(AYLO. 'Ncm 5 y"x4w>a0F 2rs-(N'IH].s8Kex9c 8T? ,b^Mx 03!1e-O^4:T~n6zvKLfk( M02u:mgljB$j"jH .CE l_KRQebC}"uu_*:bH5C3C$a_=Cv~+8C6i03jRt QVH,H:"!3-WB bLM|J#06d1A.@19?|518q0  YJ3; k}z[ O !'n^Sw7><wtr5L4}JD3q\c eQ@ /HO[}5KzL xkT" furqwsfm_mbPm ^n\XJOP>GR?A@AEC>E:80&3%:5686-,()20;.4>9.%$-C;C)""!@=.8,   2<39*-/(-B+)6' %$9*)1 "! % "# ("$!,$&$( #! F,-")*   +"!%#'"!  /!# 1,:A'3!: $+(3"+8%'./4#<&41(1##$3&1!(:A*H70,,&#,(,7:6>99=.+O4G&18./D:BDC?HKRA?HL>BAFFL4@RYFTT9MANNDCLIM^VMKENNN\]iUC`RbTNVVNJONqbxsoccQLO\\PKzTXQTNcSM[AvpUoWCBAKe`Y_\UmTbfOfo[fj\GSioYYm}tcffitckkmqq|eoomodwkkwsnjvwzwpppkknkkaOffkqffffqAkpppIt \AKpcp@c(pK],gap} 'px:XR2wHt Y6\|\U;C_ZLpytG82d2Q9yAp^8TFM!,H-(8/jp`t{ p=%q4[pf]ppwgXOpp 4sppw:RyTw!1P<RN: QGK?Bm38ESTeR0L%YnM6?PA*'.$ ,!*6 @0AN ' '$/B!8!%<0&!_&/ /%%2#2' 1E''1" $%&%4 O%!#-'#%. #"%!/B.)!F#+&%&"   ' &K8 >6(,0=)y%$E+'")+4..#$3Q&.&)A<$)%6.(6!3!"+F*!%-9(0(@]u32(F7-QR<.E3./0C^^t3EX%&G0Mc=2By4._Wo2>YB"lKv./>0$#4,s"^C2[TG9.!Q5c=\\PN~QDYi9D&r:V_Rq\Ycvg#[miM0P5ilM[B;xFi>R{YYgpqFq;ATTTGTGTATCGAGGTTGTGATGATAACACGTAATCACATAATATACGTGCGAATACGGCAACGGATACAGTTATATCGTCAGCTTACTGAGAATCACTTTAGGGGGCCTTCCTTTTTAAGAAGGCAGAATCGCCGCAATCTCCGGACTATTCAATTGATACACGAACTCCGTCAGGCCCAGCGGAACAAACTCGATTCGTTATCGACACGGCACGAAACGATATTATAGAGAGTGCGGTGGTAAAGGAGGGTAAGGAGAGAACCTATGAAGCTAATATTCAAGACATCAAAAAGGAGGATGGGGCAACAATGAGATACCCACCTACGGTAACAGTGATGGAAGTTGGAACGGCGTTCCAAGATCAACTCCTGTAGGATATACAAGTAATTCTTGCGAATACTACGGCAAACTACGACGTTCTTCTTATTTTATTCGTAGTGGGAGATATTTACAGCACGAATATTGTTCTTCTATAGTCCGCCGCAATAATGCAACGGAGGTATTGATATCCGATGAGCACCACTGGAGAGCTGAATAAGTATGTCTTCTCTGTACACGATAAAACAACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 14:54:22 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtdASPtdB1PtcB1PtcBCTS2BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 6'lNTDATA 6'lNDATA 6'lN-eDATA 6'lNDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEJLAST~~0LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS P1AM:tP1RL:t P1WD:t P2AM:t)P2BA::.PP2RL:t%hPBAS::vPBAS::<PCON::PCON::PDMFTPDMF PLOC:t$PLOC:t PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPL&&TSPACAgSPACSPACAgSVER4.0SVER 2SVER,,/Scal@Scan@TUBED10Tmpr<UserphARphCH7phDYbigphQLcphTRphTR2This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtdASPtdB1PtcB1PtcBCTS2BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 6'lNTDATA 6'lNDATA 6'lN-eDATA 6'lNDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTab@nFVoc@]FWO_GATCFeat//@.GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEJLAST~~0LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS P1AM:tP1RL:t P1WD:t P2AM:t)P2BA::.PP2RL:t%hPBAS::vPBAS::<PCON::PCON::PDMFTPDMF PLOC:t$PLOC:t PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPL&&TSPACAgSPACSPACAgSVER4.0SVER 2SVER,,/Scal@Scan@TUBED10Tmpr<UserphARphCH7phDYbigphQLcphTRphTR--)ob2=kY?Z8Z~]`m2W@V{%e_dQ<,N-AT$L8zX!3xn/+ O]rM701`jFB.VVQ8il6jJ}B~xGH$@iQ{nW)\lZhs| nG\|8 f&hPV)W)R6nL,rg`g6 ?63_`EzWkp$=RyS4*7rtlq8&LabDvv'O` JECce3YXjld96"$M@>b|U2mC!KCO`{A[55[#G=n-iw87[%)4#,u6A|D2-7gm_g({]/AT^ogV|=[P[W0L'q I`X/13U`l${\Q`O96|3Q$kv@1=,Q9\qwHN0&)Bk+7{lZpKrS|YYw>xPqL6`nY?}xj/99xvT+F4$T9tR3==QC0^,!Sk2%vAfIq}ubsCnHmVNPn2uhv]qoU)m Pl: ,uk&q6X+9mMyTAFUTT3T&^%FyPC,_,qz7A*k|_LhvHZ&qIl|pSrS';$ iD]UBLY7n nk0WC.%obB`!3<-,RIlVy8J9UTBe$-s:goT|0\d&POhAFDHYWm;2FVDp,CD4;,x8yT,I)rw&^LoT.i792C|5( J!Eb4;,}&v-T)D@4ir$GG\}d CludrV|gwCiOfFc':Z^j?F?hECel7B]%j'kjWceOSx+C!K}Z'BNp{ e,&o.jrm$v5GK:.\_'[wA#Q9Tc T-*<^k'nwi5'S;.xhKn6g;X6YMI){wclQ[>A{%i/"a-cZ,f*F8B,}|O[nY?KPQh3f9bz14S@:sZ4GrsNs~X$"Ow6qQyTo OqH~|g8SDrDum(]k@l'Z9dp"iK]jTj.uFgq%,NKo-jE(_y"%_oU@tC]2ZE<}|Uo%Z=r*r`sEY5_}4]'b5 ]{7HFwMzR93o^b,:V AmE$ww*{we*V&'Z9iS/oYFi$t.&"L4#,u6A|D2){|_nTJL:,aXj`Q Dx6m}>7:I1~8U'W33EZ'nO$`j|"/WQ`Q`zX>ykI0o6Gzv&1NmbcnP_[O~}<"&eG1<@O(LxCmpD0,_03gPV]O8oe])%|2CWllme[OCy KJFU'.QHT/}]66{6sA bBT3 #pJEa#;{U,Z4Ei5TT3T&#ONx8DTR"S"E<\AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtdASPtdAUDTNB1PtcB1PtcBCTS2BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 6'lNTDATA 6'lNDATA 6'lN-eDATA 6'lNDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTab@nFVoc@]FWO_GATCFeat//@.GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEJLAST~~0LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS P1AM:tP1RL:t P1WD:t P2AM:t)P2BA::.PP2RL:t%hPBAS::vPBAS::<PCON::PCON::PDMFTPDMF PLOC:t$PLOC:t PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPL&&TSPACAgSPACSPACAgSVER4.0SVER 2SVER,,/Scal@Scan@TUBED10Tmpr<UserphARphCH7phDYbigphQLcphTRphTR