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***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR CQUVVWVVUUTSRQQONNKKIGFDC@?>=;87643 KKKDDN__ruu  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6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 1dd04bea752f46a7b653d5efdb2dd84cSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-02_08-50_07223130Seq16779134781_134781mfagan96-Well########"#######"###""##########"########################"####"###"##"####""#"#######"######"###########################"########################"#########################"########################################"#"""##"""###################################"######"##"###"##########################################""######"###############"#########################"##################################################"####"#####"####"#######################################"#######"#############"##########################################"########"#################"#####################################################"#"####"#"###########"##################"#####"###########"#############################"####"################"###"#######################################"#"#"##"#####"###"###################"###############""#######"#############"################################################"######"#######################"############"######"##############################"##########"#######"############"######"################"############"#########################"#####"#"##################################################"##""###############################"##################################"######"##"######################################K1318_G10_Rumex_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7zWZ_hs||/Oq&XTDN+u 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BZ")V7$U<  FV?)  2<)8)j9*#S V1+([[((x&(((P1HU(((A(([Q(((([&7[rrr+@@@*tZu+ ^IIIrrIII'g@@@++@+rr++r`IGIIo!Ir36$r@IIIIII(*IIKPIr+.rr+@@@3@I+@@I@ 0@I+rIaIIppp'"lp3Mx,DP9o#8{:26qul2'l`oh> *0~kM%(UZ?5;*I"{Ip68@pttiH*( /q~Cc7= I^}VPmYM@;yC9`}@X,TGH!blt29`\BPRIppH](WYASB>F^p26n=$/ap|$pVd?pd,d5h  _N_~0 s :VkYFZ]"49+,\G@QIJv3 ~b,YM+I?Z'j96([jcGO0xg>_fOznKd8FzLHusE+z{ TcD\P^moUcP"VeJhTKokZ}/vwzEwrO~~~nl[:wLu4C,Bv;-;o uj_~}X]s:0dJ-ldZI'eyH>2v|nhXoz|7YHfWq8kc*H:uOxHkBd? p]t _dt6&G4+ ~~GPm{d}uaKRN>,^ @eqURiQ8z{l ,cF[~y~^q%H6d++VxCpoSL"T&1 1J hhAAAAAGGGGTAGTTTACAGGTTGTGATGAGTATACCTACTCACATAATACTCTGGACACTACGCAATAGAAGAACCTTATATGGACGGAACAAAGAGAAGCACAGCACCGGGCAGTACTTCTACAGTATGAGGAATCTGCGAAATAACAAAACTATCCTATAATTCCTCAAACACGAATGACCCCAACGATTCTCCCAACAAAACTTATCCACTCGGTAGGCTAAGATTTCACATCCATTCTGATTCTTGATTACCCACTCGAAATAAATAACAAAGAACATTAGCCGCTCTTCCAAGACGAGTAAGGTCCCACTCGTAGCTCCCCACCTTCCGTAACAATTAAGTGAGTAAAGTCCCAATTCCGCGCACTCTGTACATTGAACCAATGAGTATTTCTTGCTCGCATTAGCCCTGAGTTCTCCGTGCGTGTCATAAGGTGCGAGGTTGCCCCTATTTACTTTGCCCTGATCTAAGGGTTAGGTCTTTAAGCAACAAGCTTAAGCCAATTCAGGTGTAGCTGGTTCAACACTCTTGAGGGATCGCACCGACAATAAGCCCGAGCTAGAGATTAATACACTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-02 10:37:46 -04:00AEPt@AEPt@APFN TxAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSiBufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA =zTDATA =zΟDATA =zHDATA =zDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANEDLAST~~h-LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS=P1AMCSP1RLCOLP1WDCXXP2AMCadP2BACCeP2RLC\PBASCC?wPBASCC=4PCONCCM PCONCCJPDMFTaPDMF=PLOCCF@PLOCCAPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT 2RUNT  $RUNT  $RUNT  $8Rate 0RunN""J2S/N%<SCAN@SMED SMLtSMPLTGSPACAhSPAC=SPACAhSVER4.0SVER iSVER,,/Scal@Scan@TUBEG10Tmpr<UserphARphCH=/phDYbigphQLcphTRphTRρThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TxAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSiBufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA =zTDATA =zΟDATA =zHDATA =zDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabxFVocxFWO_GATCFeat//wGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANEDLAST~~h-LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS=P1AMCSP1RLCOLP1WDCXXP2AMCadP2BACCeP2RLC\PBASCC?wPBASCC=4PCONCCM PCONCCJPDMFTaPDMF=PLOCCF@PLOCCAPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT 2RUNT  $RUNT  $RUNT  $8Rate 0RunN""J2S/N%<SCAN@SMED SMLtSMPLTGSPACAhSPAC=SPACAhSVER4.0SVER iSVER,,/Scal@Scan@TUBEG10Tmpr<UserphARphCH=/phDYbigphQLcphTRphTRkl{Zq\-4/CeG1{?{sb: MOsh9VMR5t(jICx@VFmYK3AQZ`K'H:{GKipM`{`3e&Vc:z x:H-p9bgh`U*{Ga$FwXAr:3%6AB$AvG9 b?$o0)]j,XXL(y?<&jb!a72BZ|7.N[2MM@Z#bv]Z`lbkF3(jY`VqQIxwcL6-*pfFUeiM/+U*yxT/4f!Z56N'?k?~pmxRVQQ/^idT@CzS[~k8N'O?6pkC:J|bU,iPis|KRaD678w!lVcr,,z^qXnF){3&H9n9f}~E\\&(6Emc9tp] j MfaqQlYm.bBT9zH'zeE^O{U,\)lOY%}WeoVL6I-In(c$5?!,wS:@zB+(>CW22Q&]Q#V>Ax7cXF$~y;|>EY|3.B|ux).u7z"$-m8)FTk2XS"j+OC5wz^`Mv,ZD-_p;s_|g+V3SKo)67:I1@ -Tr+ TW>qO9`}!&RW8X&tG98PbC.Ju5%2-*2$G]zu7I]?lin5&OkG>:U$R\ey;3i-jE(_y"LAX;:,bQ2a5qO Jm}Pz%I!|sp:W+DO]h^5Q1V?ORJedjY{mwYBT3 #pJEK^;S4fT{-*<^kY{zsyN}@*ohj4TIx*&`_M(_yb;E2Yh^n@2dP1jpJ6~=D:W}&O[n/bsDkFhwD~EG7&o.9OQJMrVq}d7rLyEq28B]C\]A@r9I$sY;Fi$t_SF`|zo;LY&05OPh~wA[h@t?"\=QtAcfu?*M~~+N@c'v+gK >I57%6C}B-m@7t{_6p>z.\_'[wA#Q9U,Zy6Ka;v8e7s]jSHM6FNmMOx^.|bEdGn~'_2go6M/N:R; g#2L8hUCe-V:1"]/Q;[^*!t p7swnqR9F![pBTQ7u2>D8}QhyW\j&1?I| 9I+VWx;#2ps'Mv.h.9q8!0b) e:=Kna9W&a:Oc1o>Zz%8+!3@*7_62n`4573[NC~)9OO)Yr[q?d4}XiGOHKR!hjqiH(IidC4"|QHxe:[=udrV|gwCiOfFc':Z^j?F?hECel7B]%j'kjWceOSx+C!K}Z'BNp{ e,&o.jrm$v5GK:.\_'[wA#Q9Tc T-*<^k'nwi5'S;.xhKn6g;X6YMI){wclQ[>A{%i/"@>oW~!.eHB,}&s-@M+9mW'>YP~jM.9Wl[Z,9AEPt@AEPt@APFN TxAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDT$B1PtB1PtBCTSiBufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA =zTDATA =zΟDATA =zHDATA =zDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabxFVocxFWO_GATCFeat//wGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANEDLAST~~h-LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS=P1AMCSP1RLCOLP1WDCXXP2AMCadP2BACCeP2RLC\PBASCC?wPBASCC=4PCONCCM PCONCCJPDMFTaPDMF=PLOCCF@PLOCCAPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT 2RUNT  $RUNT  $RUNT  $8Rate 0RunN""J2S/N%<SCAN@SMED SMLtSMPLTGSPACAhSPAC=SPACAhSVER4.0SVER iSVER,,/Scal@Scan@TUBEG10Tmpr<UserphARphCH=/phDYbigphQLcphTRphTR