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$$$,,,554=<<DDDLKKSRRZZZ```gggmmltsuyy}~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 51e2ce53c6844b0e9f04bab4e13c71aeSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_10-50_07453130Seq16806134900_134900mfagan96-WellK1317_G10_Pinguicula_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z@GUj (GbwwP4&&-45. ~od\XWXXUMA3$ &&#!!&+0320.,)#)A^~$Y 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VypN,+RMq22=:,.-[XG4wb83 Z^S1rq u y7Cs>Q c&][E73G}~z8 :GJ)ynG()Z$Vj<3%)PDq? Jo*)lZAx ~]R\nvsGRJv>SVmW=2JNcF^* zz%hmi-SjyG!O_j$q(gxn|stB#ZfshI&Nm%GX &`W88PuI\xSHLap!r0&Rh"E{XNHt-<c[d/z.\KNh("n63iXt1#!4L&6E Br\UK~8LpxUr%3'=eh8wSf#Du'/N]T]_SgBqU-b})e5,]$$us}dxKCLPTCclVk}P#XvwriW^+u:VDYl (fLS0&@&lNNYR8|5A3pke1L]_*9d`E-B[UwxfVs4U7g2o$xh^j27,dL n[MR@l G?v;GAGGTAAAATAACGTTCTATAGGAGAGCCCATAACTTAGGGTGACTATAATTTGCATCCTTACACCGAACCACAAAACATGGATATTCAAGAGCCAAACCGAGAAATTCACCACCCGGGGGTTCCGCCGGAAGAACCGTGGGCCGGCGTTAGTTCCCAATCCTCTGCTGGTATTTAGACACCTATGTCGGCCCAAGGACTCATCCGCCTTGAATGGTTCCAAAGGCGGTGGCACAACAGCGAGCCTGGCCTCGGGGGGGCCGAACCGTTTATTCTGTTTAGTAGCTAGCCCGTTAAACCACTCTGCAAGGGGTTTGGTTCCAAACTTGTCTCCTTCCATGGTAAGAATTTTAGTTGGGTGTATAGCCCTGCGTGCTCAACGTCGAGGGGGTCTACGAGACGCTGCTGCTTATGTTAAATCTTACTAAGGACTACCTTTTGTAAACCGAATTGAGAAAATTGAATCCAACGAGATAGGTCGGTCCCTTAAGGAATGGACTATTATACCCTGAATGGGGGTATTCTGCATCACTTTAGGGAAGGGAACTTTAGGAAGGCCTCTCGGTGGGAGTAGATATAGATCACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 12:38:02 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtTASPtTB1PtSB1PtSBCTScFBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA <-xZTDATA <-xZDATA <-xZEvDATA <-xZDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEDLAST~~aLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS62P1AMHM5P1RLHHP1WDHQP2AMHZP2BAHH_uP2RLHVUPBASHH8PBASHH6ePCONHHF]PCONHHDPDMFTPDMF6IPLOCH?PLOCH:PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%6*SCAN@SMED SMLtSMPLTSPACAhSPAC6BSPACAhSVER4.0SVER c;SVER,,/Scal@Scan@TUBEG10Tmpr<UserphARphCH6`phDYbigphQLcphTRphTR0CThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtTASPtTB1PtSB1PtSBCTScFBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA <-xZTDATA <-xZDATA <-xZEvDATA <-xZDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabqFVocqFWO_GATCFeat//qaGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEDLAST~~aLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS62P1AMHM5P1RLHHP1WDHQP2AMHZP2BAHH_uP2RLHVUPBASHH8PBASHH6ePCONHHF]PCONHHDPDMFTPDMF6IPLOCH?PLOCH:PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%6*SCAN@SMED SMLtSMPLTSPACAhSPAC6BSPACAhSVER4.0SVER c;SVER,,/Scal@Scan@TUBEG10Tmpr<UserphARphCH6`phDYbigphQLcphTRphTR(w}Uzrl@O(LxC[>({]>REd7gbOHG'~[ABMNT@bxj[Dbt6XhVx9zG@[76gg`qC+lkfc40-fKbVU}MS{p[v[!s.#g;X6YM'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ-,Cz#Z|#e1a_y`[qD|3DG\TcpP.!3WN#4LdCh2d'jw~| imyZSH,6cXM1voDGG5)ljD_n?Kf]4'Uw\Pcc{\A_}$A>AR5Z(>B|::zWTR0Xwt2)lOY^k42g|5 x*.JIc\<:!:B_;e"goKC"WgQrG[MM`$M(P@>/D?*Uq$[-o9D4kij7!}WQ`QEJ9."G>}O!:i1At*ldAT/n@6TdO{7+VDSHM6FNmMOx^.EW<#G@njH[=K{_"#k6l2WeH?W~jH((nN#qvGq*1m.YHa&kWgw/|9vAMV^@Yo]p&qn~+lU,YTl_{1#p{XPTF}cyy3of)`|!-Vt2`\wmj773Ku\@\=5NDzU4oD7N**s$[-33.BkS7> YlGcRb[xy8J9tG>udrV=y`6@WBktK3c*6?FYT7~U+U*rR_"qaf/h7kZ5UPh_,zi8*$IntM\vjm2Jelv-!m!@lhP0Br[ ~L/JX;MM;8'i2{q6.>`A #pJE@^*\vJnPk=o?eU:g;TaywK[*oCf`y6n?2x`agv`k=;A{%i7#W yZE!e~8WY;`.pr$,.[G57'9\47.e6bZ( iu2Zv&1`2h`_`&1hE;qT%;5]U'V[,A4#O|!faiw`6wPjgvG9 HtWo0U:6%4sIe0