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  $$$,,,554=<<DDDLKKSRRZZZ```gggmmltsuyy}~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2c852d5ccaab41379c379da7853c6bb1Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_10-50_07453130Seq16806134900_134900mfagan96-Well K1317_D6_Indeterminate_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7zuuvz   ;`  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Ja,.F F aP,I4> ,AaHA"&P#JS>"WM$BUH$USX#B Z )T,CM`(U(;"L'EdR+a@V#OQPK3VVU4+#b01VKTVPV)TF!3^`P`PE\MS UR(U1CE1EL/CM`&0P$`'!G<0 D=]CL, FM !2.& + 7LF +( cGHB" Y% _6b5G(FF]B_ 9&dN6`( L 4O bIK1`41S=%(Y[.'@ U'NP D94(&1:02$R5=.P Q C<A1:KHA9, NQP *MWA/4_/-35' &VC/L,SE?A#U0F_U+]9@& 1?:`L61NJ1-GL2U&P7:R ;0HId-LDQ2 ,&K5NY 5 Z'+86HA551X>F#=H6:.P-I@E<I;/SO CZ5)X7 )XaQ.MF*NH_$FWV^S4 D`,>A2A%*_] Cc5 >6>MU*N HE2{F #ER!(H)Ya{Zj?tz #^ ^{Be8;L7q#wd DulI+"yMgkWv|RA`\ .(A"H5~/'z A5TjRH ZWL / eDaVt<.hRJGBg#s~'By>B5/k+S o1i E_w`N!uQ$4`UJ [Q}}*( 1q6lCXVqwj >y%HXc$ZfONc@ ZQS>%<KL2^|9'&0B>y;6\"h;@tLa#JI_68#0mYx_RR[o0P<ml~)JfE8Y "./gK=P h Zh i-B=+@_5J|q[iXlk7\cRX/bDB;4Cx$-l+ -$" % =8         <          8 &?& 7  "+4"# )- * 3?604= ("5,  9.Y/!% 2':CE2#' e V  v l:Npz@,0xdDf5fFx//el8[m;jTOk,\c:K G<{^T(<k27d6nd!]CZnlWyv 2)/5Tq(pln9!"Js !ddXJVi, @@,Ip`zd+n,,pkVQ)<Kmr,8!6L8P[ upLfp,ZQg.m:5>d 2*pEVK'p,{:'pr<X $y3(.w[-p{6 #1"p]O&rBGG6r<Ml} )/)G_<"tKE9 VPS.q_C=#S#R: @B;;>jg HGuXi:@D',!4';4'/#&&1(2S1"; "%B3)"4F:X=')57.4)" 3D&%;&(#(''#1/R@#.-O3&7/?E:A;)&*#'722[Q"* ?( +*UguFL117%&"M5-'4Y5.$(<6M&'%&.+.E-WbB]N0# %L=9F!G(<1l9J=@8%'J-"33a2Fb3;<dq;AGDG0G"0&*'N5-1#%&,AH@<uSB'.#81#F&/(:8#V+f[.5$ 1.U(-DD74A/5114$4%IET9I*Lk90$*19#`:O%!(3F#4?91"*B+F+4E% DG)G1Q,=3s<#B-3?FB:5##-E41a'%;2)'&3TA6V@=%.%JBI6;QV/$1#UZ:200%5$0'!FDP,Z_b-VYB[Dn%G-8d/:O/|b%C(FALL|l`C{Y5S+GAAAAAAATTAATAAGTTGCTTTAGCAGAGCTCAGCGAATATTATGACCCCATTTGCATCACCGGACCTAACCATAAAACACGGACATACAAGCGCCACCCCGAGCAAACCACAAAGGTGGAGTCCCGCCTGACGAGCCGAGGGCTGCGGTTACTGCTCAGGCCTCGCCTGGTCTATGGACAGAGAGGTCGGAAAGAGGAGTTTTCCGATATAGCTTCCTCCAAAGGAGGTTGCCACCGAATCACAGCTGCCTCGGGGGGTAAAAATCGAAGATTTGCTTCAGTAGCTATATAGTTAAACTTCTTTTTATGGGGAAGCGTTAACAAGCAGCCTCCTTCCATGGAACGAACGAGACTTGGGAATTCAACCCTGCGGACACACATTTATGGAGGAATAGGAGAATCTTGTTATTTATGATTATCTTGGTTAGGAAGCAGTTCTTGTTGCCGAAGAGATAATAAGTAATCTTACCAGAGATCAAGGTCCCCCAGAATGGGGTGCCGTATATCCCCCGTTGGGGGGTTATCCCGTTCAGAATCCCGGTAGAGCAGAAAATGGCTTGAGTCCCGGTTTAAAGGGAATACACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 12:37:39 -04:00AEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt:ASPt:B1Pt9B1Pt9BCTSfBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?XTDATA ?XDATA ?XStDATA ?XDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE*LAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR,P1AMJiCP1RLJdP1WDJmP2AMJvP2BAJJ{P2RLJrkPBASJJTPBASJJR_PCONJJbePCONJJ`PDMFTPDMFRCPLOCJ[PLOCJVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%R$SCAN@SMED SMLtSMPL!!TSPACAdSPACR<SPACAdSVER4.0SVER [SVER,,/Scal@Scan@TUBED6Tmpr<UserphARphCHRZphDYbigphQLcphTRphTR.This is the confidence range *ABI_Limits..FVocAEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt:ASPt:B1Pt9B1Pt9BCTSfBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?XTDATA ?XDATA ?XStDATA ?XDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE*LAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR,P1AMJiCP1RLJdP1WDJmP2AMJvP2BAJJ{P2RLJrkPBASJJTPBASJJR_PCONJJbePCONJJ`PDMFTPDMFRCPLOCJ[PLOCJVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%R$SCAN@SMED SMLtSMPL!!TSPACAdSPACR<SPACAdSVER4.0SVER [SVER,,/Scal@Scan@TUBED6Tmpr<UserphARphCHRZphDYbigphQLcphTRphTR/o'zbMaQd&79?OkZL9,,vk1G&M6] 7UkAxBd?DSGwvlAc3_{#RX#W+BcK;o`H9T+a:WkR,uKsK[NHu` *R@_AiG3mwYgE@f9kf'C#/\+F\%_krjZUTw|bT8Poo=[/19~{yrA]#_WfOMt_X`@kjJ}B~xGH$i*8j|]]u.ibu@Qhiq|fq:#6D:_f7o,f9r;qBAK$M@at0<EkP9bA:>y7j] !3xnv_YDkrD5pYy7,Fb[2yy>T\3>>B]'p8Npd-(bcn23i7FbXtrw+6Z=}n=0:E~(0B_dTh|r`=/'n|vu~CM#Y\V&WhIdp"iK]-p@O,Cj~W7.5k|_M%h'_17a;A{%iTK(>V3jo>E7-8rj~n.It!TQ'O1^m?e({ln}sFZ~0^Cu4Yy4_^ZBFf?0p=P#/0AbhE%L(!f|bEdGKI'_26.?bFW_E7bp~4o-D7I 9_[*?bCDkrD5pY^d,/IQ}{sGitGKXW .CTNCwD(ftyYZFUI8-W=;OF>BrRL8~f)NKo-bEd9gP`gvG9 b?AvQtb'J!>yH~~!|s&4)D@xG57'9\47.<<_r[)PCy5"igxd}s9?Od+k'"5-z]G3o6Rl6%4y^.EAx8aPI^QJH){!9Y2]2f1hu?K2FtSEH$*YYo%@rs{6`VsIeJM*{B]9[ikI0o6Gzv&1`Z=kY+-^tG|JWoa-s1tob*oCfvn}4|Md'ac_` WV|N&4MM8r|iwQJk3[`RFn.e{:^kkSOj!Q4KK*flVy8J9tGHrd+"L&d&2<\d&k@6GZ]]C)TiSOpP S!oS{Rn[-Vt2`\wmj7ar?}O!p8Npdpu#Wc4hAZG|JWIKT+}tr2LxCmqL}4|Mde_'61Qve])%,8=0?})jZ>zir8rP p'Ic62ir$1.XH`HO5QqE`|mwP@rB ]:]HE^OXv&nP_[O~}<"&eG1h3/[zGq;)y6}c8dgp3^|o:Xz zFA\|5DhECel7B]%jxxsWD@xG57'9\47./D?*&qDp-o*Cwh1nk-~4Kb}wLD7X{Mf4-gAEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt:ASPt:AUDTB1Pt9B1Pt9BCTSfBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?XTDATA ?XDATA ?XStDATA ?XDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE*LAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR,P1AMJiCP1RLJdP1WDJmP2AMJvP2BAJJ{P2RLJrkPBASJJTPBASJJR_PCONJJbePCONJJ`PDMFTPDMFRCPLOCJ[PLOCJVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%R$SCAN@SMED SMLtSMPL!!TSPACAdSPACR<SPACAdSVER4.0SVER [SVER,,/Scal@Scan@TUBED6Tmpr<UserphARphCHRZphDYbigphQLcphTRphTR