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  $$$,,,554=<<DDDLKKSRRZZZ```gggmmltsuyy}~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 190c396181504fd0b216db5be50c9bf4Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_10-50_07453130Seq16806134900_134900mfagan96-Well$K1317_B10_Pleopeltis_mexicana_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z7_K{7\ %BbS+!))V5$"+9DIH?2}rvtljli^G) |ybUU]hprojip~ 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d pL/Pgp-YW%$7 pV 8.DH"RPnd-Gpupw8Va,pw*5`fr=~eD 7M/[kgd-)A!;C<M0D3,#@iOH0B8Va:-EkBv?<AV?v&ce*."9:3AM.'$67% $*&-FFcMP:"3554F@,!%(#(%=Q!k.DDDC?$%0D8X@+>AU=9%%+D3.$EG;%A0!(*/%.4,'S>.#09%*%;' +10.4$0&$..#<4>*2LD#.Q+15*%! :0$JH %#::!0,&,#)&)$!>.D*)M60\9&?'(,LL2+6+ -)!*+!&*,OLEP36#1/&!,!.>+@268.-$(F7,*H*(3%!(:* .,%!"2 +=61$);rT%4V8k7a(A,+>*!%2@sC2K(%5@:]D1vtM_SQ%I "/e2{fpU& "%0P;G$ ?E@#:\27/PP:c71G+&h#3+41"51(-(%3*2$;3&51X!cX`8@M&(!-6?7\;)6do9(8Qt 7-`GOHOLe@rV&d:htn'WR!},I( bp#^CCAAGGAAAAAAAAACTTTACAGCAATAAACGCCGGACACCCATACCCGGGTTGAGATAGTACACCAGACGAGGGAACACGGACCTGGGAGAACCAGACAGTTGGGACCGGGAGGAGGGGAAGCCGCCCGTAGAAGGTTGAACCTGGGCGCGCAAGAATTCCTCGGTCGGCCTTCGAACAGAGAGCGCGGAGATTCATTTATTCGGAGTTACTTTCTTGGAAAGACGCGGGTTTAACAATGACCCCTTCCTCGGAGGACATATTCGGAAAGGTAGCCTCCCTATCTGTCCCCTTGAAATCCTCTTCAGAGGCCCCGGCTAAGACCCAGCCTCCTTTCGAGGTGCAGGTGAATAGAGGCTTTTGGGTCCATCACTGACAAGGTCTCAGTAAAAAAGAATAATGTTCCGGTTTTTTATATTTTTAATTGGGAATCCATTATTTTATAAGATCTATCAAGAGTTAATAAAACGATATATCACCAGCCGCAAAGGCAGAATCAAATAAATCATGAAGGGGTCGATTATCCCCGACTTCCGGCGAGGATTCCATATTCACCAGAGTGGGCGGGATAATGGCATAATTTCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 12:38:11 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtdASPtdB1PtcB1PtcBCTSXLBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA :uTDATA :ubDATA :u?DATA :uDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANENLAST~~VLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS+8P1AMHB;P1RLH=P1WDHFP2AMHOP2BAHHT{P2RLHK[PBASHH-PBASHH+kPCONHH;cPCONHH9PDMFTPDMF+OPLOCH4PLOCH/PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%+0SCAN@SMED SMLtSMPL%%TSPACAh[SPAC+HSPACAh[SVER4.0SVER XASVER,,/Scal@Scan@TUBEB10Tmpr<UserphARphCH+fphDYbigphQLcphTRphTR-5This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtdASPtdB1PtcB1PtcBCTSXLBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA :uTDATA :ubDATA :u?DATA :uDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabfFVocfFWO_GATCFeat//fgGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANENLAST~~VLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS+8P1AMHB;P1RLH=P1WDHFP2AMHOP2BAHHT{P2RLHK[PBASHH-PBASHH+kPCONHH;cPCONHH9PDMFTPDMF+OPLOCH4PLOCH/PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%+0SCAN@SMED SMLtSMPL%%TSPACAh[SPAC+HSPACAh[SVER4.0SVER XASVER,,/Scal@Scan@TUBEB10Tmpr<UserphARphCH+fphDYbigphQLcphTRphTR)=8m|4'v;L`-6Lrj~;b3?8r**5{H\V'+io.qmawA.&P`O_'?Z2ZR0Xwtej_@qW8SWXM=4T*RG_C26R&w^/~!Kp8&O"tX {1tp2s7}Z&[VGkB*Sig]r;a4xG57'!>&7.{MJWT|+adJsLl&pvedsgbk1^J.(KC4!7`h?&03BSmv^*:U[*WXWo0\,$bnsYK%FyPq6!oSR{[}7&0AS_7WX773V[R"\"@-bWF'zPz\]G!3@*7_6; 0|&6X`??}@8jaA]!lZbCd]s:goT|gmktayr=u4[PngD]jTj.uFr~o[|_&agD~jg6j{th_B$e_4ppOxU&g_n&J;s$dUW@_G0:_y{vr$GifM}iA{m.OcXbo6GJwmv[SN3&uLi4KZI\T(k"T'o)6yHAj}=