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",,III^^^{{{&&&999DDDXXaaavvv      **3>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR}~|{xvtromljgecaIOOOGHHdde{|   %$$,,,554=<BDDKKKRRRYYY`__gffmllssrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f7929c6ef0524d64a889e91addda0785Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_07-00_07433130Seq16805134898_134898mfagan96-Well K1317_H1_Indeterminate_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7zqtz'?IMQcAsW2fL &Fhe=~;6BIjWF06Vn d.k +<@=9:?C?/yRvGhO6pN:101/,*1B[t{`:4NYUH<7<GOM?)0ERWWZf229Y\6Z6}smpF_;gn1j#S{\QYUK9y]#z3"yRDBC>3$    3kRU(s'b0 $%        <zc uWa!9x/           %/2+ s"NSGwr+    S[u9r     2Chklyo;  %.33.'    .;@<. wB;}MF        XDD.;sqR]  S8Fz~-   /mav<j9 iKO{N         yKz=(G e{Ha?u= S:.cT4   /VY L   .^PIpOU  # $X93`5   ! 5q0F;T&  (Y0alJ7tD[wA9* l bJId WL    ({ byK_p*  (]&P ;n u9  I8''=S*  Y)Gn%  $&!             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('% H1:I0-DN+/MOP+?Z='N&KAT%230I^K\;MI%H6HML7*%^KA&(ZGN-GUKY-`J56= F/TF4> a:PW ?`:KZY[ 7[JSNC3--7 95/TCRCU[+!/ 5%T]aAP 3DF3/&J%-$+S_XQc@2B[.#9/4['F6-HF &+9 GJ,@bXZKS^V) D Z$$6CZPQJ)!Z 1)Q`[I- T.K<VF 23/JG4=T?AL=YB[VSL LN\<I6c(!( EN0T ES8WF N!4TdC?$B)3W =ZV0)4I < Lb8#ED[(b'5 2 A60;#_.\I*@F%OdS)I:V8 ?"#K 3 H [DFC$YU$C?a,9\'(* O+ S;069(J&V*4O b 1O>BM\a,[4>+ 2!;K;$[V) MV9R# %;9MQ2BV'-P7X]!/UCZ7 I8=M [ Q1C=U2'SW2Q@$ < `-2WL 9>rR6F5zG+sP6Cd,\ d 'a <D*;EJ<ct'/#2$RtAR!5XUhea'F Ef^S edqxxgm;a Z#pSk5)yY;d%rWX5jsB*=pmf~ JZx87l~fTeVwz?aMs-bt!k?Q)BERT&T^uj1KKrtt tTvT GY2J`[1BGh7+b -pS~ow[#!I[V% L-ok]Tu~O"9 F'Vybu&l{ow!$^`*ON@TEF KTP/e$?R]ZU* 'UhG0?0> / "M/p}}QjX1MC`X=T'BY21<3F4Y5(,1B) , //: $"      *7 & 4   .$ 1%   %5K.$E 13.<)!5"(-$-"##@%5:T5K%5'Z"r&=%#&E1&72##c..&&1##&###M# #Hp)M)E7u-Zk#[]7 pRwj4ud{=+@Qt8'diV41Bgk gbiUhYrP&[a*r >YvGuT\ |9i  }4|9dNVzH[t.'A,6:HEG)]R ?Hp,EI: /J,HO( Dug,T ,>W8B JBY8"O[px!f46Ms<pz(b$p-s)dJY(dupwW2R'-BWdkQY,:D p$wdE#f ZfOpa zn~b!^r%.GEUC;\*<3)"RnO=0 #&'I%+'@?5C9)"#.(2%!.,_2 $ !!0236g)>Mf3* %-/#5&!; Ib**51;56G,*:K9#,J:$*L73%*-%:K54!%.C"3&,$/*pu'/33!'08#)"4 '*#*<-Y'`+,,:#"&0<'.E?M/7$"$'>%s:!'Q!#%!B!/7J(='$"0,3*%+06$31g24$43K. @n)=##<.+7<%v-T0$I.9/US<4"9)&$d2651:%0-,_B%,]%$6, en+K6DD72lS0%!0?6H#G*7MD/R4c%9-:5IIb(595."%,@$#63-\#&5+A@=D@U*=!36AS=2/C+3&2,A/$!Z-$AF':ACD%`%!;56B.8({"!YBb\Ui=X*oXFI!PZ+736,6Q)4i$14A0KvD,xCZQs Nt[j)v^]<VSxhI) \aml$g@AAAGAGTTGGGTAATATCGAGGTTGTGATCGATAATATTTAGACACATAATATACATGAGTATAAGCCAACCCTTACTGCCCTATCGCTAGAATAAAACGAGTTAAAACACCAAACAGACCTACGAAAGGAGGAATATTTGAAACTACAACCGGCGGATCCACCACAAACACAAACATTACTGTAAAGAAAGATTCTCCCAACAGTCCTCGTCCCCACCTCCATCTAATCTGTTCGACCCAGACCCGTCATCGAGGAGAGGATTGATACACCTCCCAAGGAGAATATTTAATTGTTCAACATTGAGGCAGGACACATTCCCACATGGCTGCCTTCCGTAGAAGGGAAGCGAGTAAAATCCCATTACAGAACACTCGAACACTATCAGAAAAAAGGATTGCGACATACTGAAATCCTAAAATCTAAAGTGTTTATTTCAGGGTTCGAGGTTCGCGAGATGTACCGCACGAGGATTCGCGTTTGCCGGTCCCAACTTCGAGCTTATAGCTAGCCATGATAATTTATTTTCAACACCACCGAAAAACACAATTGCATAGGCCTCCAAGAGGAGTAAACTATCAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 08:47:40 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtyASPtyB1PtxB1PtxBCTS>BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 7oNTDATA 7oNDATA 7oN3`DATA 7oNDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~=VLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AME(P1RLE$_P1WDE-sP2AME6P2BAEE;P2RLE1PBASEE|PBASEE7PCONEE"PCONEEPDMFTPDMFPLOCEKPLOCEPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPL!!TSPACAoSPACSPACAoSVER4.0SVER >SVER,,/Scal@Scan@TUBEH1Tmpr<UserphARphCH2phDYbigphQLcphTRphTR6This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtyASPtyB1PtxB1PtxBCTS>BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 7oNTDATA 7oNDATA 7oN3`DATA 7oNDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabM:FVocM)FWO_GATCFeat//LGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~=VLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AME(P1RLE$_P1WDE-sP2AME6P2BAEE;P2RLE1PBASEE|PBASEE7PCONEE"PCONEEPDMFTPDMFPLOCEKPLOCEPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPL!!TSPACAoSPACSPACAoSVER4.0SVER >SVER,,/Scal@Scan@TUBEH1Tmpr<UserphARphCH2phDYbigphQLcphTRphTRTFo@L15@FT!sz^2h3)-I561hB_f*>0-D%~{a,vT`S'MS{xCM&mUYa?Z?Rq}n{if?K;@WJG7jVYmW=GNx_|$xzW,DK >M7NJrj(oJQi)-PA)yw(~?V'i~o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*Ml$.9@_05a=gHFhwD~ ')&o"J'8O[,?a9'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ1r]C$p~/Ykf4;N^YEu'+=3yHt.&T, /Y+!mfbhP}7}_'AIh_`V$];9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,IlY'_2Acr]t>OE2(/(W,XD3J6EX! w+[_3sh("r1'!$n`VZI;P>W+YYo%+0|5Q1V?ORJedjY{mwYBTU;CxhKn6[MHSVi220b_'{aCA{%?_CjFDhECel7B]%jxxsWDy4<|O{^Mm[*&`_@-|T+a6oGhnWe^kQtA+UZ~en?,}&Dg8YauBQBhwD~EG7&o.jax]CR"lWW .C&qq<6(m=':Z^u4|8e7s+]i@CHbB&H+J-]Sl!&Z?DEUW?|QG/N'4;M&bX*F8Bhrb8EY|3.B+0T{!Xf] _ocT)FTX>j3XV=K3]1wv;{?kJ:h,r4[-T0M5[S*.J-j~W7.5k|_-S_7}_ooUzbaI23|5njUZ'KD7I 9xxsWDZOv'5d7`Ed,{w.P&-Gt?E>@d&r&iox~*4fC\*WiEa>i(\&?11@o[,<4zIx}IaA9!IP45F1)o6/t$1|5O-U[d>7:I128)TK7<4Zm[E,fcd]R03DiU3o XSXW .CTNCwD(ftyYZFU5|8e7spFp[O6h"4#O|!lYqdqY7_twvr]LaiY2]2r-U[D~TWrSNmWT/@{K m}{H1}zIeD8zEXTM~ykI0o6Gzv&1NmbcnNl_a?FQPU{mwY1XV7:>JWT|;B9t-yc=3E;qT%;5]U'C,CsDa)xz4|Md?]R_s_beB Ri/"@BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 7oNTDATA 7oNDATA 7oN3`DATA 7oNDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabM:FVocM)FWO_GATCFeat//LGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~=VLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AME(P1RLE$_P1WDE-sP2AME6P2BAEE;P2RLE1PBASEE|PBASEE7PCONEE"PCONEEPDMFTPDMFPLOCEKPLOCEPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPL!!TSPACAoSPACSPACAoSVER4.0SVER >SVER,,/Scal@Scan@TUBEH1Tmpr<UserphARphCH2phDYbigphQLcphTRphTR