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  %$$,,,554=<BDDKKKRRRYYY`__gffmllssrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 3c338609d7244b569b852b8f409397c5Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_07-00_07433130Seq16805134898_134898mfagan96-WellK1317_C12_Triumfetta_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z3ANg7`~LW6m)^fQuwEsjaN+rhjnfN)o3\'vH"  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H-K` =8]IR Qb#bI!XC> 3O%7`,`7 L4 =,TES/U?M]Qc&J_ -["@S5[O:%?0H#XV2:F+]);>8]Ra61RZ.=>4-G]C)dN:[%@@45TD=HMUV->W %'c*BW :]""DDS9&1ONB_!:$JF! @H1Q $^V>TA3G[ZU=ZPMI$DX OC)("J;1'+;9U592 d!8Z% ?a(U)F#Q`#-8*SK/D<-.;#$B\#&#(7<'2?+ _H=D2 acc3VV:6>`J+%IUWB!I*, F@E+%U>WE2FN`!?^$T "4Id1 E%\. %\c\R>GHHZI.P4WUAI9 IK 0D+@ 7D#A `%L&@CR_>+@cH,@+R?Z^CBEIX]JYTAV73R0b8!5 ]75SR&=_G%N#>88XS= .(E)a>'G$V 8R0))+5O *O! ;Q4IKDE <T#N-W  !'4aG}xzedl7Ny_B.Z6gf [BV}"903 hB$iWRw<rLWwo c6|jn?$+-.gzr$iap>gO')E*6f&HdN#l4YJV5C@=la<}PV\R`ad i(3+{Fn >VM,ifn_jx?qN(Tc6_`Ly\U^D-v /p4EKl$ygf B7y+3qq;FHEV?B#qd(R <U P+lWlVQ rq(:h@t0c$f- _^kT} HY5N8ohh V@ d f H5& (L8!\'2enpv\lfVZrKLm@aDTHR7:L=Z%=8/$7 YB)7%!*"% A5   >       "  $#%   ' #      "'       @#  !  #   ;-5 '$ 55 4  &%*(.-?&4C, & (' )+ ''7#  7'7#.7-1<O.11@%6/?)8%%Z!4SR>*HQJFGb32-2HHBBB99;GGC]9QC*'%O(NRN756)CL}cAG< 4 :)CJ5/g\#U_(L7_nNah99GB9999BB9BBMp,*p pGB.p|_dFH />p,#d}g6+6. d8p!o#,4 tKK:npb(f,cy8R:I*JlmQIpAhpF"9E,|"gd[ IS I65U8!]8xJd#)>UI5P;eVK7-4)&<e<)HZ/F"4#K4)<%.+;&A,62&Q@BWf-=K&!B':2(O)1"$*&be[.>)6L#I9)4G&5"2"6>32\@34'(=!*1)%+6(9 +"/>1B00G)+2>?9O7>?^1<;9,(3:-9$%:(e)S)E.77>DOOWAA33W4)$@L<5 .(Qd+%#(, 2$c712@gdK,E6/".@+&34%3u:'02P?.#"*8:337%U36>2/BF''*3@B$-=70B6(.D-(!4#9H)'A.$>.l1EP"C.a;Xe$0^;)Qa-5/Q=0+-T-:dnjee]#N~,/>~Ke+Dr>sOJL,_3B"jA)Xt.BiMm:+=$dhFgs[^E<w=B3 NZCNIV:O/.S68>Eb|w\z.j-kAWLMmAWEUP=%MsTJ-c*DA7Z:rycy\N\-sUVy_Ms<t6t|<V@QazqJw2\0Di>1;1AGGAAGGTTGGGTGTTATTTTAAAATGTAATGGAGTATATGAAACACATAATATACGTCAGGACACCGACACCGATACACCTATATCAACAGAATCATGCGAGGCAATTCACCAAACAGAGATTCGTCAGAAGGATGACGTTTCAACGGTACTGCAGGATACACCAAATATTTAAACGCCAGCATGTAGTGCGGCTTAAATAACATTCAAGATCGACACGGAAAGATATTCTTTTACATCGTTTTCGGAGCTTGAGGAGGGTATCGGGAGAGGCCCCCCATCGCCTATTTCACACTTCAAAGCGGAGGACGGGATAACAAGGATATGAATACCTTCAGAGACTATCCTGTACATTAAATCCCATTTAAGATCACCATTTATAGAGAATATGAACGGATTTGGTCCTCACCTAATGGGAAGATACGTTAGTTTTATTGTCGGGTTGACCACGGGAGACATCGGGAGCACCAGTTATGAACGTTTACAAATTGAAATTGGAGGTTAAACCTAGCCAAGATATTCTATTTGGGACGAACGATAAACCCGTCACTGAGTATACCCCGAATAGGATAAAATCACACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 08:48:57 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtvASPtvB1PtuB1PtuBCTS;BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 7FnTDATA 7FnNDATA 7Fn1DATA 7FnfDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE\LAST~~:LLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AME%P1RLE!UP1WDE*iP2AME3}P2BAEE8P2RLE.PBASEErPBASEE-PCONEEPCONEEPDMFTPDMFPLOCEAPLOCEPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPLTSPACApSPAC SPACApSVER4.0SVER ;SVER,,/Scal@Scan@TUBEC12Tmpr<UserphARphCH(phDYbigphQLcphTRphTR)@This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtvASPtvB1PtuB1PtuBCTS;BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 7FnTDATA 7FnNDATA 7Fn1DATA 7FnfDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabJ0FVocJFWO_GATCFeat//IGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE\LAST~~:LLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AME%P1RLE!UP1WDE*iP2AME3}P2BAEE8P2RLE.PBASEErPBASEE-PCONEEPCONEEPDMFTPDMFPLOCEAPLOCEPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPLTSPACApSPAC SPACApSVER4.0SVER ;SVER,,/Scal@Scan@TUBEC12Tmpr<UserphARphCH(phDYbigphQLcphTRphTRTFo@L15@FT!sz^2h3)-I561hB_f*>0-D%~{a,vT`S'MS{xCM&mUYa?Z?Rq}n{if?K;@WJG7jVYmW=GNx_|$xzW,DK >M7NJrj(oJQi)-PA)yw(~?V'i~o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*Ml$.9@_05a=gHFhwD~ ')&o"J'8O[,?a9'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ1r]C$p~/Ykf4;N^YEu'+=3yHt.&T, /Y+!mfbhP}7}_'AIh_`V$];9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,IlY'_2Acr]t>OE2(/(W,XD3J6EX! w+[_3sh("r1[!h$sIQ8otJTly]r.*x_[c|_nl>o 8:T3?qm#vRRC(vJayr=u4ZfoQe=N^tpu&ofW)NKog~!-|r< wvr]LaiY2]kO~JcW`$~~;`.pr$,.[G57'9\47.SbXW,62DmOYX/D?**s$[-HbD:/~sN91`zKA[Lz2fJq3IxdO#rTisB)~HF#O];*;[F"~(,oc7(/Pi?Qlm T.+6P-2ZE<}qjM>Pz;+hj3jT$3o%+0|5Q1V?yRl<`QV-AMV^@c O*Pv