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AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-23_23-51_06513130Seq16681134623_134623mfagan96-Well!K1316_G12_Indeterminate_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z $),17@INPOMMPTVTK>0% !%('! 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NC,X++$NM;-G- Y S .0N TX"Q0XDRP112T78D**BSI,\=X c4.WE5:,%"+ b"PZ>> DK5EIL 53CBL: PEA^LM/%70J["4]2J5\:YL8`< -`IWbUaBY!Y]1X c 3@F.<O;7HM7;G5[ >GD-W[(J/(K"2%-Q8@XKI <_.4HR S 7^E#A;M"YVcJ Nd4!Q <DV?`D *$7-@:[J"19?N>$ 2=7IZ39J a< '@*,La dL!R0J=#R5@JbKSA9 (':QC]*I VO^cL*CO1 ]=8'a#*P/ W:P2d\HD#!5FaN%6 PYCB^57OT8SBE^DA >)2P$(2IA126VB2A09Z[A Bup>"faagc)M$+$.M>} E|xwgmgl$io{umdiw&c^4$@<[M'rz6s4_=r @UxO!^ R{C TltfbR\!u"vTK:( )AK  y6KW& <yZ7sn`/I[/s)wh5X?>k*D.B_!W*y nIl,&HOq9CWO.G+JT%N~T)H^1 {G;\= L/XE|lVq}%A`j~\ o5LC'dMB=&0c<6j{otTIJ_F +/vy!`S(MpV- 6g`g'Fx\!76-kBVw^k2&&s{J!JL0zZmRF!s{jywoY:NVGT>H:5BCV?3(B;>!"0307.3*/-2                  2  "                          )*/))<#**&D"  !&"#%)="&-%21.&''/=4(23#39'F6':43*1/(&GJMU?;<E>)b5-5=!"1>R.2;"Q;O:>==4<@.3^7@K;NMMR=tC7.GPMDTTXFECrnILZXQhWEXJ[nFNOn\ZIWD9KcSZV]pW^P_Ij@@@0GGG'GRRRIR?RRppppup";9pdQ&K:L,8o [6AW@3DS&]+ ^(?p{ u((EN XlR@np, BOn! pC,~3xTDL[lQh({ Wx ><KZvZdZWA8>dQ\g\@w(p_pQ&pc 'L,~#q4TEZ GmpMpt4wzRia HIp__Qppp+kv+]<Z8k,< WD <6 V6amQ@ KI{_`L%:o?LJFOT@8\X%2/n+vCbZ#+"d., 2.3?:"cN@.-06>?K5X.:8P L7=1)pD!(9:%,6DT(:5!!!ES3BFO]`*6+=.V?.)FE6,&=%X%<9=0*0 +">e/C@--)2g<H?=@9=#8,Ed];7G,$;5(.+;':2E"B+sI$C2(?}nC|S),5&@0*4:%4%D0?'576T%C,HZ:O$+ <?+0C3=_5%9VS7?!3G*/;K<<fGrEMCHs-,!>C?/v4P!6k_:*#-3a=R-O3z);L.8"C]8'3Z8!3807''`QU1-9/*3K-Ab>=K<6-D;DR~o-":<o0)P8=D=4[[-I3>~Z]1L`3N<Q8m%H@O'.L2.-I=q)?V_+I7[{_<>6Q^DNe#BVBXF=E -..C*9^-5O92Y7!NT:)n;F7gL47F4,>_!@91,bSHz>e6;*?re{a,O,B+O!GAAAATTAGTTCTACACAGAGGAATAACATATCCATATTAAATTTGCATTCTTACACCGAACCACAAAACATGGACCTTGAAGAACCACATCGAGAAAACCACCAATAAGGGGTTCCGCCCAAGAAACGGTGGCCGGGGGTGCGTGCCAAATACTCTCCGGGCAATGAGAAACAGTTTTTGGCCAAGGGACTTAACGGCCTTGATTGGTACGAAAGGCGGTGGTACCACAACGAGCCCGTCCTCGGGGGGGAAAAACGATTTATTCGCTATGGTAGTTACCCCCTTAACCTCATAGACAAGGGGTCTGTTTCTAACATTTTAACTTTCAATGGGGAGAATGTATGTTGGGTCAACGGCCCGTCGGGCTCACCTTCAAAGAACAAGACGATACTCCATTGATTATTTAATTTGTTCCAAGGCCCGCCTCAAGGGTTCCTTATTTAACAAAAAGTAATGAAACGATATAGGCCGATCCCTTGGGGAAGGGACAATAATATCAGGAGACGGGGTATTGGCAATCACATCCGGAGTGGAAGCTTCTGAATGCATACTCGGTGGATGTAAAAGAGTACACCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-24 01:39:33 -04:00AEPt?&AEPt?&APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS{BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?9~rTDATA ?9~r-DATA ?9~rQDATA ?9~rDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANETLAST~~y~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISNP1AM@e>P1RL@`P1WD@iP2AM@rP2BA@@w>P2RL@n>PBAS@@PPBAS@@NPCON@@^~PCON@@\>PDMFTPDMFNPLOC@WPLOC@S>PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 3RUNT &RUNT  1RUNT % Rate 0?RunN""JZS/N%NSCAN@SMED SMLtSMPL""TySPACAaSPACNSPACAaSVER4.0SVER zSVER,,/Scal@Scan@TUBEG12Tmpr<UserphARphCHNphDYbigphQLcphTRphTR9This is the confidence range *ABI_Limits..FVocAEPt?&AEPt?&APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS{BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?9~rTDATA ?9~r-DATA ?9~rQDATA ?9~rDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabbFVocQFWO_GATCFeat//"GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANETLAST~~y~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISNP1AM@e>P1RL@`P1WD@iP2AM@rP2BA@@w>P2RL@n>PBAS@@PPBAS@@NPCON@@^~PCON@@\>PDMFTPDMFNPLOC@WPLOC@S>PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 3RUNT &RUNT  1RUNT % Rate 0?RunN""JZS/N%NSCAN@SMED SMLtSMPL""TySPACAaSPACNSPACAaSVER4.0SVER zSVER,,/Scal@Scan@TUBEG12Tmpr<UserphARphCHNphDYbigphQLcphTRphTRPF{ytl+hcDW4KuYGH[{O!QECN&0@]/0dwm\e+<}P?sw!iOup_3:E-v^gMs7M9{SHM6Fnf%)qk&YgDS7}_'AR;L6,8efj+l-uk#%C`]@zMxLrLei;E]yKoS]8qUvn}4|Mdna'"`ko`^ kg"#]eV&U)>`V2]<|':eqB?kgP<((7!+43BfZv{i.mcRFJ9'ngg61C"wY\V&Ip?Hds:MkO@JC)RVZW[Z!f]ZE!Q;eat6LudrV=y`6@vulc*{7=hI8J)T(k"T'o0_s2#O|!Y<,h`+m&wE6Gz zF/"@>oW~!zFHyErN3W4KD@w JU6PX `HO5QfOl>o XSudrH>PWYQIxwcL*L@rIwYWgUTjLj.u%xfmq;MmN2CgGz^nLoT.i:"\oE):|j!]3B68l|ap'Ic3XkSnwkI6 +FwMzbU`AtGbI.J:*yEwiOOd&79?Ou+:n%ra$NwBx6Ks;6R&)Y?Z8Z~9}_{J^z(aT20p/(ZA$den?,}& b!a7QAQ>Ube\W'KFkaqQltX[3>>B~:^gWiD!}%`;pS8muLy#U9KUEy{V(cO9+!!f|bEdGn~'_26"nlG;|>k0|3.B|uxPR~{z7_lnqR&GX>2Z <}EGCN8mgmktayr=uzF4/T(zNpP S!oSR5*6Y?_MBSmv^LoT.i,ZikPvypAX~L=?-XPKw+[_g4#q&N7u`GcO581W+V|BO(hT*"?sNA^D4wJH{lQpEX D%"E(Yk{Hys<7qL}4|MdwYc_`k:!.tko2KWtAcP@>/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;LP1RL@`P1WD@iP2AM@rP2BA@@w>P2RL@n>PBAS@@PPBAS@@NPCON@@^~PCON@@\>PDMFTPDMFNPLOC@WPLOC@S>PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 3RUNT &RUNT  1RUNT % Rate 0?RunN""JZS/N%NSCAN@SMED SMLtSMPL""TySPACAaSPACNSPACAaSVER4.0SVER zSVER,,/Scal@Scan@TUBEG12Tmpr<UserphARphCHNphDYbigphQLcphTRphTR