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"                     d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR=TTTMMMlll&&&000999BAAKJJSSS[ZZbbbjjirrqxxx~~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ead487c542284d37a8b9cb3042e1810dSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-23_23-51_06513130Seq16681134623_134623mfagan96-Well K1316_A2_Vismia_baccifera_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z-[ ,2)":[=[s bOSg~s[LPevxqT3hK98Ik.\w{2No NW7VjjQ{s)A$J|i[QJD>82.*'# /D_}Awf}XB70S,%G-o|(Wop\25]_lD-'*.( hNw8F9!@V]P.Lob\YTI;,#$/@OXUH7)!!&+.-,/7ES]^TA,%;Tm}Du0]pjK #5X&&Ah*y2Wt4s!|_QQYbjnnkdWF3  ,34/++3?KMC-A1!,~ { o@#  ._ h@   +?`WhUaCX^<fW 5|ss{mQ2 !&#  &,.*# *0.&!*0224@^m7 3=Lpfjrx}w\@'    ?'i~QrJ+'..$e} i\2:|to'/pB<{1t{M}xvqkl|Zr1 .* 'g",Xt^VW_irwvn`M7" %-0-&!")4AKOMG=1$ &00&4PbcT9 1COTRH8) #5SrdNPv7XD{Il<5<:!Q* xu|zovu5[3'*.-$   _G,]Q )g2 bC-mxO0R/  K]qIxB!+/+ !-1*DAGS2Rw|kH# $A\q}~yodWI8& 2H0;?q0r8|> bH:;GXhqrrz'IkRb]% ( +Sw~qv}%Pe9~7y@5*sOo40Izj/S @q-qmwpYE835:?CC>4% 8>Hb8'0WUI-%f>fH ]g4(2AJG;.%#'.2/$D/|z{tQ):TQ99Ns^9'*?ck; 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"**$  #!  -;EKH>. ,@LOF6$    #*06;?@>92( vN^G@AA@QAKB.bcpKB_3730_POP7_BDTv3.mobtermGGGGAATTTTGCCGCCCCAACCGGAGTTTTTTAGCAGATAAGCCTAATTTAGGTTTAATAGTACATCCCAACAGGGGGCGACCCTACTTGTTCAATTTATGTCTCTCGACCTGAATGCCAGGAGGCGGGCCTTGGAAGGTTTTAACATAAGAAATGGGAATTCGTAAATCTTCCAGACGCAGAGCGCGCAGGGCTTTGAACCCAAATACATTACCTACAATAGAAGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAAGGGGTAAGCTACATAAACAATAAATTGACTTTCTTCCCCGGGAACTGGCTCGATGTGGTAACATCCCCCTTTGTAACGATCAAGACTGGTAAGTCCATCCGTCCATACCGCTGTCCATGTACCATTAAAAAAATCGGCAGCTACCGCGGCCCCAGCTTCTTCCGGTGGAACTCCAGGTTTTGGGGGGAATTTTGCCGCCCCAACCGGAGTTTTTTAGCAGATAAGCCTAATTTAGGTTTAATAGTACATCCCAACAGGGGGCGACCCTACTTGTTCAATTTATGTCTCTCGACCTGAATGCCAGGAGGCGGGCCTTGGAAGGTTTTAACATAAGAAATGGGAATTCGTAAATCTTCCAGACGCAGAGCGCGCAGGGCTTTGAACCCAAATACATTACCTACAATAGAAGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAAGGGGTAAGCTACATAAACAATAAATTGACTTTCTTCCCCGGGAACTGGCTCGATGTGGTAACATCCCCCTTTGTAACGATCAAGACTGGTAAGTCCATCCGTCCATACCGCTGTCCATGTACCATTAAAAAAATCGGCAGCTACCGCGGCCCCAGCTTCTTCCGGTGGAACTCCAGGTTTTGG -3<EMVbkt~"/;DQXgs|*8FP\iu~(4BM\jv#-8DP[eq !.8CMXdr}%1;FR^hr~ !+8CP[gr| -8DP[gs~   # / : E R [ f r ~  " . 9 C O [ e q }  " . 9 G R _ j u   # 0 : F Q ] i u    # / > H S _ l w *7CPZit~(4@MYfo{ &0<FUblu ,9EP\ht.5BMYis~ *5BO[gt*6CQ]hq -3<EMVbkt~"/;DQXgs|*8FP\iu~(4BM\jv#-8DP[eq !.8CMXdr}%1;FR^hr~ !+8CP[gr| -8DP[gs~   # / : E R [ f r ~  " . 9 C O [ e q }  " . 9 G R _ j u   # 0 : F Q ] i u    # / > H S _ l w *7CPZit~(4@MYfo{ &0<FUblu ,9EP\ht.5BMYis~ *5BO[gt*6CQ]hq       !#   8,#8;      1  ""08!'"1   "$-+$""+'!""""")""'.$;8"$-&1  """ 2'"$) '%#""&"'-"""($"""' """        " "$"&  "%   "       $&     " "  "%        !#   8,#8;      1  ""08!'"1   "$-+$""+'!""""")""'.$;8"$-&1  """ 2'"$) '%#""&"'-"""($"""' """        " "$"&  "%   "       $&     " "  "%  !.7WW1#BKY6@!$K V0S] )2CN.D#+@ ;RA?,%T8)bK@H?(/^Q6L=_'D!F<0HI!!7X7FB6@<N=D?9*5BF-S%IW^TNB957#(_,C(092YY=;":7]+@F 78#&0KM Q 8NWV9VED.>=_2=:+MS^BX5I UJH;0 K!B8G4E"TV !B:Z,Z<, (]U;2DEL D6`7 ;;"D(\E&HE8-Q"/&4EM$*]R]W)6^+@S7!P*!(&GZ1 T Q QL''6*4@4'4c/$X^!a6]aY7_ RC. &=(PEI7b8!]*1\0ZLY./Y2;CPV4=RR P*S>(,>[;;A@ UU$9*%\XW+T0ZIDE3 JM;A=H. =w@O iEv_; @78z)Tp0#0HC>\Db I C9/z|a}7v )Y4_L~fb/auI('8C{B_]Ne-w]/)#ma)V } r$VN2g`1p|2X~M0b>5y y-48b^ {Q|aMRhKNHv )*;nM>OR/n2ceVlsA4dEe&r"=mz" S\P)u5f!*KfQ}av`h K4 v 1Jj26%yn^9 >-qF)9hhiN@? (81&X#uVWZBw{Q^y"?O)2l?4P,Mh6&`=KN .+<( ?;>LL <W m4' XC7D- ,   4  HY H)^*& E (** 2 "7 0* A   A17F67C).-l55Pa". D)-25 /7!^3$9 YF#9(!_:HG05 j{'M ppC8'HK}i`/A[rde7:,*gCHrF_6?2,8{?eXh1?EL=V 0{@ SJ 0YUpcmpb8[S3.p6"0.t<@Zj,*na5'4$b%(phO8C-vf rlP0p8r.O=",,&z2ddKSu=$I(yi{qt|2Q=GG&<5  ;dkd-4 K;+L_!=? ; 4x ",m`J]NIs $WGR"[lw@ .P 1.m;'q%:x3l/nd,iF2 dHD]AF ;?n^ f^D"@ZAJf|wPPgAHQ_h!zI?Il~4wFN= :77 wO+9f9W/b|E%Sdj)Dm7q; r7#m2]r]n5BxiwK; W'Xjy r<//5.W+|o)kR<R Ld$TAAAGTAAAAAAGCGATACCGGCAGAGGGCCATAAGATATGCAGCCCAAATTTGCACCACAGCACCGAACCACAACTAACGGTACTTAAAGCGGCACCTCCCGCTCTCCATCAGGACGGCTTGCCGCCCGAGAAACTAAGGGCTGAGATCCCCGACAAGTCCTCCCTTGACCTTCAGAAAGAGAGCGCGGAAAGGGGTTTTTACCGATACGATAGCCTCCAGAGGAGGTTGCCACCTAATCACACCGACCTCGTGCGGTAAAAGTCAATCATTTGCTTCCTTGACTATTTCCTTTGACTCCTTTAAAACGGGAATCTCTAAAACATGGTCTGATTCACTGTCACGAACCACAGTTGGTTCTACCTGCCTTCGCAAATCTCGTCCTGTATCAATGCCGTCATTCTTATCTTATTCCAAAACATCCTCATTAAGAATTTATTCTTGGGGTABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-24 01:38:28 -04:00AEPt:'AEPt:'APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt`ASPt`B1Pt_B1Pt_BCTSc)BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA =kzTDATA =kzϐDATA =kzJfDATA =kz<DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~aLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS@P1AMQP1RLNUP1WDUYP2AM\]P2BA_P2RLXPBASBPBAS@MPCONLPCONJPDMFTPDMF@1PLOCGQPLOCCPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 3RUNT &RUNT  1RUNT % Rate 0?RunN""JZS/N%@SCAN@SMED SMLtSMPL!!TySPACA[*SPAC@*SPACA[*SVER4.0SVER cSVER,,/Scal@Scan@TUBEA2Tmpr<UserphARphCH@HphDYbigphQLcphTRphTR@!This is the confidence range *ABI_Limits..FVocAEPt:'AEPt:'APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt`ASPt`B1Pt_B1Pt_BCTSc)BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA =kzTDATA =kzϐDATA =kzJfDATA =kz<DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabqFVocqsFWO_GATCFeat//qDGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~aLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS@P1AMQP1RLNUP1WDUYP2AM\]P2BA_P2RLXPBASBPBAS@MPCONLPCONJPDMFTPDMF@1PLOCGQPLOCCPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 3RUNT &RUNT  1RUNT % Rate 0?RunN""JZS/N%@SCAN@SMED SMLtSMPL!!TySPACA[*SPAC@*SPACA[*SVER4.0SVER cSVER,,/Scal@Scan@TUBEA2Tmpr<UserphARphCH@HphDYbigphQLcphTRphTRrIvMa3o;E;qK|C!K4kCqOE9`F7}LjJT*%)^`83@9rHG#-p^x5Wc7;yFc=kJn\9-s1tog,s48tNhAkZC__cIb_@U])%fD1,;0{voekHy$hb +4v+gK >M7MY4!Ye;'UCm]]?EZX/$:>ZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_RJ`GVR_6MW!waXj`Q>co6m~2HBIPKNa( r{Nbw66x|W"Ke|f^3i=_:SR[7}?e78SG9} P,hz9_"#kjFO-U[ds=:I1On(I"]lr>D>VN|UPYEFI50});oH_nA(u6Z~=}F)h>Sc*?7kx`rx} 1{T/ilVRJxG57'9\47.<<_`#6E12 d+[Y5xo|[EG\}d hfz6i8Z26bb+i/_wg=A#Q9nPk@_[O~}<"&eG1h3/[ze~!Tq*nEg8Z~d_1{63_iGbN+7F= E"ae-F*r`s#T)3KO]h^* `v?{}I8w$YWmOyl}$rv/qwnF){xD\4AFU5|8e7s`]pPC,Cl278;;9Vge!Yz]%MJe:MX 4AEPt:'AEPt:'APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt`ASPt`AUDTB1Pt_B1Pt_BCTSc)BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA =kzTDATA =kzϐDATA =kzJfDATA =kz<DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabqFVocqsFWO_GATCFeat//qDGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~aLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS@P1AMQP1RLNUP1WDUYP2AM\]P2BA_P2RLXPBASBPBAS@MPCONLPCONJPDMFTPDMF@1PLOCGQPLOCCPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 3RUNT &RUNT  1RUNT % Rate 0?RunN""JZS/N%@SCAN@SMED SMLtSMPL!!TySPACA[*SPAC@*SPACA[*SVER4.0SVER cSVER,,/Scal@Scan@TUBEA2Tmpr<UserphARphCH@HphDYbigphQLcphTRphTR