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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR;TTT;MMjll&&&00099?BAJJJRSRZ[[cbbjjiqqqxxx~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8bba002d8e1a4d1c95caaf9833168c29Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-24_03-25_06533130Seq16683134619_134619mfagan96-Well(K1315_A1_Maxillariella_variabilis_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z~~zvvy~3Pk 5_  4FTakol]A]0Z;! 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"!4*#.464'/ ! .*.41)44("6016/44*)0"'*2444%46666653-)422..1%/"2.#)2212222./,+(,+,224(*.(2*.4))(%240,%2!+2$ 1  22135!::2:66         6!65$::026#3!(.4-:41.,44)&6/4(..6>>0/>>>>>/#>>8/++33>>>>/>>>>6>'*'+''8,/6>>>>)>>6/>/**8&2&288>8882383888&&,'*88  ***%/3 %2 +8&,8833*>8&228''&'33&)2388,88888888888>83888888828>8,8 3*83'3>83828.283&*&&'83883338>/ 83*8388*838'*388323&8''38>>>>>888&23*3838&888%-'*&&&!8*3!3&88888388888*8/+68+8+""*"288888'".('*8.2.888/8.8>88888#44&44*6+461604>(!2+4/4.&44+4+4.444..2.. "!4*#.464'/ ! .*.41)44("6016/44*)0"'*2444%46666653-)422..1%/"2.#)2212222./,+(,+,224(*.(2*.4))(%240,%2!+2$ 1  22135!::2:66   NS-M>'Tc4-K>N0H]H=5G\LH,B//O\A IA?[b)J4\#L6I-O,Q7P#S3ACU6+A P@:. A@:`)V@G3P KBQ-F(2E,$(L+-C47.&GDa%! !': 02K2(82U GYB >E2"4:_5$=cB,E L=E&:NB.BUV X"?,I<FY F" % T #D2H1\dX0; =b ,c+ H?3 =/1T7-F +B^ 2;]#W!T#@EF0'B8TCa=O\BHEZI>!@!GK'0_8'8B*&O<L R,]4F! +"RN:`c.313 PFTDP"8R^+A49@\+ 8]+$W.N/F(;D72`51$JGVRF&$['=:,; 5&P?F> $2Y/ =6?7Q,<C Gb4I/S`KO0Z(=-!GL-\5LRZ48I?+-C<c+P%R6$^: 5Y0],*/aM-'+RBS(#5^/ 61b T6/#EA G*GX=/M"1RC=Qb+ , B,I>>>%* "2BX^'@QVjB7<>X*}(TaS$&Ebt9vD; v=xJt `(K)WL&2,[?`P-(u7>&7-`*~-< ~} r"6 $al*)-$v#=qi9?.+UlRj>"T n:JB(G3\/JjEkI$+53g4R8$7OswLa68$rG5 1cbB}i_}aHYoX(s3WhRor`~skFmk}'rGtKYBE# LDZb'O& jg,0te%XDr8{o6 [jt0*sTcl-GkD` |, 3h8 HN?\ri1.{ e r}|TebL]\\\ahZvoCkta^UXROXTE4NE,C1;@.*\?($ !81' PC %5#&%$#3'(%*) A?:  9?$0   !#-  ' =%- !                     ) *   &)"  !$"$ *$##%&"#+%(2$+!!%&/.(! ! )#6 )" ,0K(/&.%./*82'*'1*" +6=*389(0'$02:>;**C516A-H<<G@C%7;QEBD#4=FG;K<H@SIP@HHoE\BNQ?`XJ1E<[QT[LiIPPPXA]OlGQRdUNVxZUTY_\\\eVZ[lb_^ho[kYw]QgQig{ph}p[hdi]{raSfopnMc\\\!NNN{N_4_p__k'gp^ kpBpGe,pK@p8 J*?xzxsG)sB;&$wCgdOi%8Y6k)6m9Q V,{qH|c5W \!ptjN~ 35890aroi][bvsq~ZH"N,~B -p|_nps.lWfmD}BrLSp~8+Ut]\1I[PsOpx"gG?H<8y GpJ/)3 pnV_|Y0K&/c[\#DZ[)pT:$L\ /R$+<ql9,O /OtI3=M9H0 'R?0(><06&a )%$KW|4aKK-149=$-?+<!-[f>Un~r6&j{ 69?kVv_sV,y{cW5I:,jBCV:+kX.2ywDn&(Ts~cq|Dl6uipy^p6TNMmY[1.]Oe{9{h'o$kYkmizHkA^M|>W`gzFu0]>}J#*C=[KPpQx@M6pa_emr~)UD5cJULjWYaY_cPiO\iG1gStox~`?mW?nUe>y.^;1X ~Tan-^^_w3G8d| 1m2 2\n_Vg;t$A>@+g[SNEjVLtipx?B`{NKTbh~0~G3NWOvQmIoZF@n'K>D\Y2qF!Mv64<@P3WZlAU 9]SATTAAAATCACGCTACAGCAGACCAAACGAATATTATTACTAGGGTTGAATCATAACACCTAACCGGGTAACACCAAAATTGGACCACTACACCTCTGCAACCACGAATAGGAGGAGCCGCCCAAGGAAGGGAAGAATAGGGGTTTCCCAAATTACGCAGACGTACAGCTCGCAGAGCTGTTAGACCAAAAACTTTACCCACTCAGGAAGTACGCATGTTAGTCACCTAACCGTCTTCCCAACGGCCACGGGGATAAGCTGCTTAGGCAATATATTAATTATCCTCCTGGCATTATTGTCCTTTGTAAGGATCAAGACAGATCAGTCTACCAAGCCACGCAGTTGTCCAGGTACAACAAGAAGATTCGGCAGCAATCGCAGTACCGGCTTCTAATATGGAACCTGCGGCTTGAGTAGCGACTCAAAATGCTGTTGTTAATTAATTACCTCTCCTTACGCAGTCGAGATATTAATCTCCCTAGCGGTAGTCTTTCGAACATGCTTTATTTCCAACGCATGCATTACTACCTGGATGGGGTCGAATAGCGGGATACTCTGTGGATTAAAACATAACCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-24 05:13:17 -04:00AEPt=sAEPt=sAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt~ASPt~B1Pt}B1Pt}BCTSy{BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ? ~TDATA ? ~DATA ? ~PDATA ? ~DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~wLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISMP1AM?~cP1RL?~_=P1WD?~h9P2AM?~q5P2BA??uP2RL?~lPBAS??OPBAS??MEPCON??\PCON??ZPDMFTPDMFM)PLOC?~VAPLOC?~QPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT :RUNT  2RUNT 0&RUNT  :Rate 0?RunN""JZS/N%M SCAN@SMED SMLtSMPL))TySPACA\SPACM"SPACA\SVER4.0SVER ypSVER,,/Scal@Scan@TUBEA1Tmpr<UserphARphCHM@phDYbigphQLcphTRphTR This is the confidence range *ABI_Limits..FVocAEPt=sAEPt=sAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt~ASPt~B1Pt}B1Pt}BCTSy{BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ? ~TDATA ? ~DATA ? ~PDATA ? ~DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabFVocFWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~wLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISMP1AM?~cP1RL?~_=P1WD?~h9P2AM?~q5P2BA??uP2RL?~lPBAS??OPBAS??MEPCON??\PCON??ZPDMFTPDMFM)PLOC?~VAPLOC?~QPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT :RUNT  2RUNT 0&RUNT  :Rate 0?RunN""JZS/N%M SCAN@SMED SMLtSMPL))TySPACA\SPACM"SPACA\SVER4.0SVER ypSVER,,/Scal@Scan@TUBEA1Tmpr<UserphARphCHM@phDYbigphQLcphTRphTR]nd3ghlRc T-* +Wt3z{0bInTor@tbfD7E)0Ie/AK8N'nsv&'Y?sxWlZN.YRz!#fn2%FT!sz^2h3)-I5d~)Uk}`:r&xvr_WG\|8 Xo2h{/S9~i,EAx8amb#QJU0iHAj}W2yv!a!<}B]rQHfKbVU}Uz1p=|(LZjp7xMO9j;q6TB(2JLvuUlN1@#vQ!TMF4FprjDp*K0.]+0Oj!Q4aK{WlVO:}mldKVM bynkW{\By(VMt5+y|`}__';_/A@(#jFnjUZ'KvNIGQ5ENI@[wQi^*w% enqb[*jG3T%ycX}%z/q1wv;{?kJ:hAqTFU-vbNhsrU5-uvCbbJ_<$AJu"#phs*AWS,q+)x]k 9lWqTX8"0e-kx87($CkI+5?-Y.pa6:!.tkj^4E2rW.X}=_;!;`sEY5_}4]'Z5P%?p MfaqQleA./q}.}:>JWT|+adJsOlG6]}fWG\|J_KpdFm~;[cMmN2CgGz^n7a!.t;Z\39/hV6XD3Xm?;;DpT73@|ir$1.XHT+O}L6>U{mwY':8 vjgow^;hiksyP:r~64L&eG1H+KB6KP8)s5AVihwYc_`koGhnWe^kQtA+UZ~en?,@-nveo*uhGlB^PY6C}Afz@jXaM~63(|}jGPNYQIxw}H{-*<^k'":?S`,j.uFTc%{M*AqbEdG9}j~:HzpWI23Z;|T92xN:Ch[`AzOpPWT<;u4@"Vd*dzF4/T(wfT;GAze[6m[X1EDE?\g}_t6Lx]V==a^Y`g7yZUCab07!Gq 7'[YD\<]*:853`l@A_:k9vAMV^@=?]p&q6X+9mB\yE*<^k'eaK|`P@R(RlSR[7}?e=ES77~U?63_iP-2ZE<}|Uo%Z=r*r`sEY?bM:bFs5Iis|K/l3672tXMV^A$+vTE#!#+Xn$yYE!p;|WnT(%Fy1pLze~!Tq*nEg8Z~KI'_2B.*W"i-I7} W5WDt;x.qK|>VRJxG57'9\47.<<_