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***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR=UTTJMMlll&&*00899ABBJJJSSS[ZZbbbjjiqqqxxx~~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2f61a9686b594140b19cdc542a55abacSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-24_01-38_06523130Seq16682134617_134617mfagan96-Well!K1315_F12_Miconia_gracilis_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z04<HYn *5=BCEHOZensuuvy gI&lVA*(CdBj0AP]iqsnbQ<'~xn^J3aA$ 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Q DX0O2\H?&9&-=C,3X,c5: *E9!W=X-E9I)X=_G+1. [G2dAAYJ-4K+)'A6?@9K(8`R #:G061Q,0'. *VY?3L?OX) Q&YaL+-NO'4X/0MO*U3WTUA %U8 $QT" !G^6Q"&4&TZ21ZZ$G<V$A&@%<U=V>A01d !!QR`4 8;O(ZPT301-PK-b-;b'> 7=Q` R7\'  >MGW8D6]"I0[ *T5&5 %F0#\ 690 (PA :GU>V_9 FD4M;5IW*D5Gd%/C7:#!Y(] !,",6'3&)J=aD>Q aMQJO$;4T &5!X:2%>:b`U""S9</ G/2 ?,:M3.EAJ!b.-4-K5"FV#9$]2?NSMP- YL%755.NZ2V9Sc8c>A)QUa!2?/=.`PUkSYw{Nb*NX FJ[YrXTAX\KNQDcve!gQi k/DqQ.*~x : ncT[vPMt]^9-omw\7jmt]s`BWT1<yalm=?X_aB$L[YH`,Q=* t0qh_1* 0@B5I 7hmZ$|l3Lof3d@*E4tm$|]'~)me$CC,gz4c:"H/=ym#!L/:Dm`J1;A6-'%l 5U&:)Ze@(~'j  10@[+ ~aHw%^xc*T#jB(ory2JL_]IJ,  Y5ma9#(PSAMp!Egr\w&09yMCq~1wc1r5V^ML"" <WO;B%x9163===:%+#!4(-)/%* 2-7,!) ""+ !                                "              %   %   '  #-"("#$   #+" !"!(@$!( 0.%2!/!*&'&+)E/")18(2/3H6'B(3-02C**@31..;,@?6/A4:;F;)6B9<<TTB<53JFE7=A2;B;U5)N\LU`OCQEG>v@<\PLGNQOT:\ab\^PPFImRRgaYa]QlU\QIIbfGYR_ek`gJgpE]~J6>>SSB>>>>BblB[qp?p*vQMp7cJMQbU$pxd7 )dp 8 y)=#=,[,do8I&{ =8ddpdLd(p7R8i08\+z fpuXg!oO.qb ZF,p,QZp%,Tpd8pED8F?5S #Q&FR' R`kpp>,~mvpYPpp ;:4\4UsBk?q S#xc"$k.2u;0A/S!(%0^GxD$- %1!"$K{+ $!@!S? G& )"D<,&6(+'/|H1,5-F5:2%'")" &,*(/!&%&$("! AC!%< %)@$$/%&!(Q. 8#+"#* (%4$%*E)F:'S/0%+4 /!1&$ $$$$!)'"" &%P1$E*%0% !$3+# ?' 4@4=().$'()*?#!(.&%,1S9/M /)0#A@*&4<'!21.'"*8)#'N103J+-#$H8]N(#!S0!Mls/a?2E,,015:l+%ADEU(8" |0E=?<!T=/11")>IBVc[y>?_@99LY<KffMn?=]~I;=> c;IdnflE<b^*cUjFr+w<`AAGATGTGTTTCTCAGGTTGTGATTATAATATACAGTCACATAATATTCGGCAGAATAACGAATCGGGAATGCCCCTTTCAACAGTTCAATGAGAATCACTGCAGGGGGTTGAGTTTCTTAAGGAGGAGGCAACGCCGCTAGATCATCCTTATCCTCTACTTACACAAAGACTTGTGTGTACAGCGAACCTCCCTACCGTCGTGATCAAGAGGGAAGGAGGCTCCACCACCTCGAGGCTGGTGGAAGAGGAGGGTATGGAGAGAGGCGCGATGGCGCCCATTCAAGAGATAAAAAAGGAGGAAGCTCCGATAAGGAGGTGCACTCCTGGGGCGGAAAGGGTGGAGGTTGCCTCGGCGTTCCTCGCTGTAGTCGGGAAGAATAACTTAGTATTTAGAAATTATATAAGGGGTAAATACGAACATCTTCTGGTTTTATTTTTAAAGAGAGATATTTAAAGGACGAACTCCCACGTTTTCTGGTAATATGCAATAATGCATAGGTAAGATGAATATTCTATGAGTAACACTGGAGAGCTGGATAAGTATGTCCTCCCTGAACACGTATAAAACAACCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-24 03:26:53 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSHBufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA 8qTDATA 8qyDATA 8q8iDATA 8qYDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANEVLAST~~GLIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AM>|2P1RL>|.tP1WD>|7lP2AM>|@dP2BA>>DP2RL>|;PBAS>>PBAS>>PCON>>,6PCON>>)PDMFTiPDMFhPLOC>|%|PLOC>|!PSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT &-RUNT  RUNT RUNT Rate 0RunN""J2S/N%ISCAN@SMED SMLtSMPL""TGSPACAb; SPACaSPACAb; SVER4.0SVER HSVER,,/Scal@Scan@TUBEF12Tmpr<UserphARphCHphDYbigphQLcphTRphTR2This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSHBufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA 8qTDATA 8qyDATA 8q8iDATA 8qYDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabWFVocVFWO_GATCFeat//VGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANEVLAST~~GLIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AM>|2P1RL>|.tP1WD>|7lP2AM>|@dP2BA>>DP2RL>|;PBAS>>PBAS>>PCON>>,6PCON>>)PDMFTiPDMFhPLOC>|%|PLOC>|!PSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT &-RUNT  RUNT RUNT Rate 0RunN""J2S/N%ISCAN@SMED SMLtSMPL""TGSPACAb; SPACaSPACAb; SVER4.0SVER HSVER,,/Scal@Scan@TUBEF12Tmpr<UserphARphCHphDYbigphQLcphTRphTR]%A'on!.*R@_aA+|1hygF8y?^qs?C!e2=c-{O YXpN}5Mzi5fI[PeK}d>/JJr=*0_fs_c&a~'S{&vT+8@/S&ofW)NK[>9`ht1A{QuU[PbI^n0 Dx6m}:)G*.Nr]0;a=bZ5hf61F;K9JX@{F`!{/GC3[^NHE,v*(]4]m9pfaX%\NzU4^PrtC[J19D5mr+8B9.~;{lUz{19se0:=y=&e=1&!rEV(V-IAG&e"i/J>zN62d-I;4,,pCw|#O\mk*]l-u4|8e7s`]pPC,61h67{=Y`gI)_|_n?NhN{CwN1d^MT9HssM T:CrJ}u'rtc[j]/7<("Hf.Q*s`AtGHrd+v/,J6v$NUvc*xGZ]k'lCKOMQwKdLC2fo;LY&f&){t~1{[q?Eb,8`a)}qjM>PCNIG2gc*?7kx`rxPo"`dbB-8'i2{q6.>`o?OpOw!b)9OO)Yr[q?d4}XiGOHKR!hjqiH(Ii3C{"gQ[O8JH]}I_\Ksl(ZJp|9-$pVWM(Mu+wGkSOj!Q4X+*flVinJils>:y;`4o_=&W.: wT_no2nJBY YlGcRb[%O8{q6.>`F7u2]H!R;{?kJ:hAC.F4/oak"T'o)6 K*G) NL#ND7!90Q2w^T[O"$}<*x_[c|_njr4_y!iU5|8e7sINi@C,_,qz7z}yaih?]R_s_bed{a9_ajFO-U[dUDNxGq@p'Ic62nrD5pY^d,/IQ}{sGitG3(|}jGPNYQIxw}H{-*<^k8e7?GOV5ro{`qafE*&aF2`_c&a6';_szT/"a/hV6T]==6X*s$[-T_sKQm5APYXA\95Lq>l>o 8:T3?qm#vRRC(vJayr=u4ZfoQe=N^tpu&ofW)NKog~!-|r< wvr]LaiY2]kO~JcW`$~~;`.pr$,.[G57'9\47.|2P1RL>|.tP1WD>|7lP2AM>|@dP2BA>>DP2RL>|;PBAS>>PBAS>>PCON>>,6PCON>>)PDMFTiPDMFhPLOC>|%|PLOC>|!PSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT &-RUNT  RUNT RUNT Rate 0RunN""J2S/N%ISCAN@SMED SMLtSMPL""TGSPACAb; SPACaSPACAb; SVER4.0SVER HSVER,,/Scal@Scan@TUBEF12Tmpr<UserphARphCHphDYbigphQLcphTRphTR