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H h 7 A ;g>%tVL(k+vU4fdXXNRG8I95#-)3"!%#)  P ,,,III^^^{{{&&&99DDDNXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR3SSSLLcjj""",,+544==CEEMNNVVV^^]eeemllsts{zz<<<<<<<<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 3fa438313e144d7fbd3a6c3bfbdfe891Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-05-02_21-23_21173130Seq1027110271_Lehman_K1358_SNPMEX_rbclfjmadera96-WellRubus_sp_K1358KB_3730_POP7_BDTv3.mobSeqPop7z 0Jd{.F\p'8J\lzpV7h=^:1JeP!HmkM/znaP9pR4xld_\XURPNNMLIEA=:8652.)#!%(,.16?MazK7kq;Y%x]I=7557:>DKUdy'lxKJr~oIZ+  %N }$U@\," i|R4"  3VWG~j-FU3  #&'&$"    BW*G9x8 N  "$$# !""#$&'(('%$##%(+.//-+(&#!   !R ^= xM)4Rs6 "%())('%!   1xr"gl13N  !"!  !"""""#$&'&%"!#%&'(**+**)('&%%$$%&()($"&:f~e| e9 %*-.+%     !u VnIH<P7!    ,B1EOtzQ}oX}=]>    Q$T &i/I        *c  (j,  giZuW       GVW<u& Zd5fC ?wC"H    =1WAX   >t_RDSmA :rqmd\fcu5US %.21,# ,h&)d&  .XS'mgfQP,XwuPT/TV\0N    MIv;L U>\<JFnCgQO!ST[[' P\CxS5}a *f ,Z M+II7e'    OQc-!\YBmVJ    SuJDhS;ud Y#    <Lg`k !$$!  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(2-+#1!28 &% / !!*/ (,%#)#"#&0!%&$! $$-5+*%++!"TL( ,("(!-D#&%7#5:%,3&, :4_"<10-32:/=)HZ))(VH$9,""]D7L3@V'=)#5#:,":-CMQ<.=[*Z83[6!B !($2.Ll.,:R#*s6S34[A(QBMI>8QVEW>)4 JOjUEpXuVFw7R#@:$#$]ycR>%,>@1W)>vQh: KBB5{DH>gMsEbvKMJh1`PV$]R51kj KbGAGATTTGTTATAACCAGGTTGTGATGATAATATTTACCCACATAATACACTGCCCAATACGGCAACGGATACACCTATAACGTTACCTTAAATATTATCTAATCATGAACCCGCCCTTTGGGAGAAGGAAGAATTGGCGATAGATCCGCAGTAACCTCTAATTCCATAAAGATTTATGGGTTGAGAGTTTCTAACAAGACTCGTTAGCAACACGGAAAGAGAAACCTTTATACAGGGACCCGCGGCAGAGGAGTCAAACGGAATAACTACTGAAGCGCCCAATTCACATTTCCCCCAGGAGGAAGGGTCGACGAGGATAAAACCACCTTCGGAAGAAGGTAAAAGAGTATGCACGACGGCCCGCGCGCTCCTCCGGAAGAATAAAGAAGTATTGCTTCCTCCTAAGATCCCAGCCTACTCCGGTTTTCTTTTTTTATGCGAAGTGCGAGATATGGAAACCACGAATACCTTCTTTTTGGAGCAATATCCAATAATTTATATGTACATTTGATATTCGATGAGTAATACTGTAGAGCAAAATAAGTATGTCTGCTCTGTACACGTGTAAAATAACCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-05-02 22:54:14 -04:00AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSZBufT!B)CTIDCTNM CTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA )R<DATA )R<6DATA )R<DATA )R<NDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~XLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS-P1AM@DEP1RL@?P1WD@HP2AM@QP2BA@@VEP2RL@MEPBAS@@0PBAS@@-PCON@@=PCON@@;EPDMFzPDMF-PLOC@6PLOC@2EPSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 0RUNT 5'RUNT .RUNT 4)Rate RunN""S/N%-SCAN3SMED SMLtSMPLkSPACAcTSPAC-SPACAcTSVER4.0SVER ZSVER,,fScal@Scan3TUBEC4Tmpr<UserphARphCH-phDYbigphQLcphTRphTR3This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSZBufT!B)CTIDCTNM CTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA )R<DATA )R<6DATA )R<DATA )R<NDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabhiFVochXFWO_GATCFeat//h)GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~XLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS-P1AM@DEP1RL@?P1WD@HP2AM@QP2BA@@VEP2RL@MEPBAS@@0PBAS@@-PCON@@=PCON@@;EPDMFzPDMF-PLOC@6PLOC@2EPSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 0RUNT 5'RUNT .RUNT 4)Rate RunN""S/N%-SCAN3SMED SMLtSMPLkSPACAcTSPAC-SPACAcTSVER4.0SVER ZSVER,,fScal@Scan3TUBEC4Tmpr<UserphARphCH-phDYbigphQLcphTRphTRvTLYr*X=+I}S$PS9Hb?j[]8|a)&p[>Se4#Y`,{[._sa$_aEC0'Uo,#$.:}jD(y$9eKobs>6I(C.F22jDTWy2>y^q3Th$Y thENWNv3[My co{l(l>6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^T[PXFWhw cUaczMJ^(wD14dNbzMd&8]t?E>+Ez DZBCQnrrGM^Q:4+:_D\a$Fl@i2ps'Mvn}4|Md^_w&;H26t{_"#k<>Dj&['KD7I 95[Xv,Kho|[E,fcd]R03Wi8b9s|l}$rv/Z-\dXm4c1/`WajZUTw|]jl@i&RCSwToN6|1Dlag&<{[;NAxYX]Ln?,}&8)TK7<4Zm[E,f *"c{+cWL N*hbDk0'_Cm ixPPis|K/l3672'[)VB!d+:pZ5KRCmnlcEn=6;}S$X:VDSBrRL8~f)NK1EDE?]wJUo6X?/WZ#6E]bZ&[';(Y`[2gx+C!K}Z'BNp{ e,&o.jax3o^b,:V AmE$ww*{we*V&1h.iOWhbk1^wKB_,qz74*le_M%~'pEA({]/AT^ogqlp,4lpy]AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtAUDTllv|B1PtB1PtBCTSZBufT!B)CTIDCTNM CTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA )R<DATA )R<6DATA )R<DATA )R<NDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabhiFVochXFWO_GATCFeat//h)GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~XLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS-P1AM@DEP1RL@?P1WD@HP2AM@QP2BA@@VEP2RL@MEPBAS@@0PBAS@@-PCON@@=PCON@@;EPDMFzPDMF-PLOC@6PLOC@2EPSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 0RUNT 5'RUNT .RUNT 4)Rate RunN""S/N%-SCAN3SMED SMLtSMPLkSPACAcTSPAC-SPACAcTSVER4.0SVER ZSVER,,fScal@Scan3TUBEC4Tmpr<UserphARphCH-phDYbigphQLcphTRphTR