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(  *,=M]qWkSQ\SZE;D<-(!( !/"2   %       16+I]F\PKeN@3OG@Tll_^dI9)61%          #!N7@?AECK6$     0,:=:Z]ZZiKFOJIPKVkXLgbkTZF@>(%'  $5)/@09ACAW@5.("             #     1$:;AGDLC?Q5,#                        ) :GQYJ4=/)1#   %#<@YecXW[BA7#-I%'! 85>+:MLXDQD<))0#"      $')@=NNMA<;* )              5 OQ[d}uofiY:L-.$#"+,                                                                                                                                                                                           2,,,III^^^{{{&&&999DDNXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR$ SSSKKVjj"""+++544==@EFLNNUVV^^^eeemmmtss{{z<<<<<<<<<<<<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 45d64bf61010438194d22f69dce3e7d8Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-05-02_19-52_21163130Seq1027010270_Lehman_K1357_SNPMEX_rbclrjmadera96-WellPhyllanthus_niruri_K1357KB_3730_POP7_BDTv3.mobSeqPop7z  Ds )=K[sI0g>f1j4CMRVXYYVN@, /FUZTG6!8}Ii,\t_TJ=,(Kx2]H1W!@lcSD?^/ ojoxzqjghkmmib[TOMNPSVWXXZ_l nkyS'tztsql`Q?.^#B<wpkjnvaRK4 i5DqW :N1<@l?otQ (hWnN:/'!    +:Oi BXQ}SA X69cs!' <tZ@* $@atS7 O/4v Xy8 (@]yiR=**8EMPMF<2'    7iAZU`( !&*+(     .] qYZ{7      .g;QVN~C" !    $''$ !aV5fsg?" %+*"   (.0/,)'(+19DOZbggc_\[^chmopommnrx|z{}|yvsplgb]XUQLFA=;=@GTlZr(E  P~C@`#      R/Vh`  lCd]0uZ      !|%RwQfGJM  3rLwe*W      >5ny~1 %.58:::<>AA=830048;;989<><2%0g^9\W"  #N"^~yN[!0v9M$Ol'  @'hf)]0$b,@\6VTNO+l7l P""Q}K9)mQ3g^+V1k;WM= >0h{f.P" /Z\-   U5F7|? 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"   +!4TB)(QO9?R ]0H0: XG, C\#3( W%"U8B\-1`2MYCO3*5/+8% MB :BEQE ^T231<I6?,Ac 9(GaEZ*F dG+. =2_N< 8,FZ8@L13/(Gb48A%Q>B#: TLNX_ LS 1D(ZQV*6+SZT$786PVR UBDA"<P1J<*@,=7;NDGQ?3(?)%8G H K\1$&!Nc#1X "\<\@/*-6_+I$F0E ? +1^WLKTJ91 1+O@49M#<N/* bP6 AOP 2N U (]:M <<@D^0R+.Y3H[)V`+G5'M0 W$)ANIR ^5T.O ]%aC$DR:(%@Z": /Y;:!*C=[R N@D3 SO6;#J48"S2SOYR*S*A$D57:;O SJa&DI=:D\ 0'DA 74'!)_YQA'.4FA`JAW'45 W7<#>PC $J?a /)A'\-2$P/-/<22VNc-R28LDJP 9wxQ|W($hDwE/0)~`o_v/Z)i =MOz9vH" {L=|B 7N53- ^pl h1cGA9}7R7 9cc=p{;vzNkterB: ti(-EE@{z52t#kRz6?V T869*F([IA@adSwA<id 3B0Nge p0G G[rCv6#Nt},O1^ js"GT+s`suuOo~  ?M)+.Fz"3Y<$eb$~Oz |2OQJqM CsU`)f%](uVkK; :hkcN1d[]V~CLa iCuxa%pGNI3\7bzg6jkpz; 4Pik7>XO#x}ptbLimwWBKEIS+I47D36+<Y>6=3((,%/$ %Tj.% *         #   !#    "; >     .   &0  #%'  N3  ! $  0&/( $ %  :#1E6-4'=+=0+'@V L&B$W6 2C,W-/8L8 ()540,/c-? 28!R8D9Q<==M@B@B*!Z<%,,,))A,,=,,wp=::0:N=== :D44aCG{$JDu'K4@@DDY@@I44.>6`44@5DDY4@@-3944zfCCCw@\tFM44@4@@2C444)hmCWCCC@*B1C@@444@@D@@@ppt4d7y/,ea8'1Rp,,8:G@f;bA8daO528F[/AMp8L8pD8,m"[7H18' y %ig8 p68qb1pj7pf5p:PHig ,As#ZRDQX,p/!F*j1dS~]n7xp8d%#g {pRp;Y)z/pp v88nC'~iiCvxPe-q?4ro6(5+Q==vO\"31xMS1 JMGv*d1 X}[q,72P7$B'!$*=/TM$")6H *BG('/,#?*0&#%#)%='!'G)"1"#==#.5##%)0/:2G.R4FIO&_*vM71F(&510" (@GZ !:JCNhDR.%>?-1 *_98@T]wY11O~/$#6h3Q-75[LA<;?3.@#'*?=$F%$X- B<dA(*+.ULEU$74H9:3IfX0>8=Rt^9>A?;Y.'^A]Y_FK,D?<DFY"0)UfFU52X=>G&ZS=9;p@@LeUmLra(tpA6E$+K3K5e>""KLBp4vDdr8?zK:N=e9OB,!DMiX1a[tv1OKqV7YCm)0)E@<5Ndf{5Aq2Nnd3td>$|^MJKW`e%>jo(J[@L2Ez`,z+i3hV6P"JVpNdJ>oq,[~?J uC_DobV*)G_kAAGGAGGGAGTAATACTCACTGTTGTCATGAGAGTATGGACCCACATAGGATTCTTCACAATGAGGCAACGGATACGCCTACAAAGGCGCCGCACTTCGAATCACATCACCATCACCCCCTTAAAAAGATGGAGATATCTCTGCTAGAGCAGAAGTAAACTATAGACGTTCGAACACCAATGTGTAGAGAGTCCCACACAACAGTCGAGAGCGATAGGGAAGGAGAAGACATTACATTGAGTTTGATTGATGATGAGTCCATCCGGATAGCGACCAAAGAGAATAATAAATAGATCAAAAAGGAGGAAGGGCTCACAAGGACATAACCTCATTCCATACCAGTTAAATCCCTAAGATCCGTTTACCTTACAGTCTGAATGATGGATAAAAGAGTATTTCTTGTTAATAAAATCTTACCATACGAAGTTTCCCCCTATTGGGTGCGAGGTGAGTGAGATTGAGTGCACTAATATTTTTGTGGTGGGGTAAAACCCTCCCCCCCATAGGTAGCTTTCATATTCACTCAGTACTATAGGAGAACCCAATAATTGAATGAGTTCCGTAAGGGGAGTAAATCACCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-05-02 21:24:15 -04:00AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTSVBufT&LQCTIDCTNM !CTOwACTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA (Q4DATA (Q46 DATA (Q4>DATA (Q4rDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~ULIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOIS)P1AME@P1RLE< P1WDEEP2AMEN1P2BAEERP2RLEIPBASEE,&PBASEE)PCONEE9PCONEE7PDMFPDMF)PLOCE2PLOCE.kPSZE`PTYPIPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 4.RUNT RUNT RUNT Rate RunN""S/N%)SCAN3SMED SMLtSMPLSPACAckSPAC)SPACAckSVER4.0SVER V~SVER,,Scal@Scan3TUBEC9Tmpr<UserphARphCH)phDYbigphQLcphTRphTR8This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTSVBufT&LQCTIDCTNM !CTOwACTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA (Q4DATA (Q46 DATA (Q4>DATA (Q4rDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabdFVocdFWO_GATCFeat//dGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~ULIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOIS)P1AME@P1RLE< P1WDEEP2AMEN1P2BAEERP2RLEIPBASEE,&PBASEE)PCONEE9PCONEE7PDMFPDMF)PLOCE2PLOCE.kPSZE`PTYPIPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 4.RUNT RUNT RUNT Rate RunN""S/N%)SCAN3SMED SMLtSMPLSPACAckSPAC)SPACAckSVER4.0SVER V~SVER,,Scal@Scan3TUBEC9Tmpr<UserphARphCH)phDYbigphQLcphTRphTRvTLYr*X=+I}S$PS9Hb?j[]8|a)&p[>Se4#Y`,{[._sa$_aEC0'Uo,#$.:}jD(y$9eKobs>6I(C.F22jDTWy2>y^q3Th$Y thENWNv3[My co{l(l>6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^T[PXFWhw cUaczMJ^(wD14dNbzMd&8]t?E>+"M|w#wpd10k:*9cI+DXb\f%\uDm`s6)\R5y=Y`g%gcmea_';_/e"^=0UA!%EeKOWQ`QV-@[3>>B)u:[owQC(vJnPE*F;2_/gYFi$t_SF`|zvn=Y`1&!AuJUo6;^F6@_=!)}W}oo-o;X^`>EY$y!TW3nG6`VX+*fl{iG>A.8X:B?r@i$!^h{we**,ACT;fQGE-`x&i)6JWT|*0_fs$X=<;#(":?S`,j.uFTcM#yKA#2rBA++mqIlEN&w|Tc$ LwG1*Q4NB (_e:RJ4ir$G(~}d M]f)C^DZ=<7:cmj^k-T0M5[S*.JpxcSX`"lef5U_9}|Ate1}1"/bW>y&bC]LVn!<|'k0|3.B+0Oj!Q4X+*flt78{u:DATA (Q4rDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabdFVocdFWO_GATCFeat//dGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~ULIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOIS)P1AME@P1RLE< P1WDEEP2AMEN1P2BAEERP2RLEIPBASEE,&PBASEE)PCONEE9PCONEE7PDMFPDMF)PLOCE2PLOCE.kPSZE`PTYPIPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 4.RUNT RUNT RUNT Rate RunN""S/N%)SCAN3SMED SMLtSMPLSPACAckSPAC)SPACAckSVER4.0SVER V~SVER,,Scal@Scan3TUBEC9Tmpr<UserphARphCH)phDYbigphQLcphTRphTR