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S S S K K V j j """+++544==@EFLNNUVV^^^eeemmmtss{{z < < < < < < < < < < < < < < < < ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 177374a3038146b888d30b7f3d92dd9bSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2017-05-02_19-52_2116 3130Seq 10270 10270_Lehman_K1357_SNPMEX_rbclr jmadera 96-Well Indeterminate_sp_K1357KB_3730_POP7_BDTv3.mobSeqPop7z "4H\luvqkggny
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AATTTATTTTGTTTTAAACGACGTGTAGTGATGATAATATACACTCACATAATATACTTCCGAATAACGAGACGCGACCTCCTACAAAGCCACCACACTTCGAAGCACATCACCAACCCCCCCTAAAAAAGGTTGCGCAATCTCCGAAAGCGCCGCAGGAACCTCGAAACCGATAAAGGCTTATGTGTATAACGCCCCACATAACAATAATTATCGACCGGTAAGAAGAAGCCACCACCTAGAGGTTGGTTCTAGAGGAGTTCCACCAGGGAGATGCTCTAGCACATTTTCCGGTTTTCAAAGCCCAGGACGGGGCGGTAAGGCGAACCACGCCTCTAGAAGACTTGGTGGGGGTTAAATCCGAAATCCTTTCAATGTGACCATTGACGAGAAAAGGATTTCTTCATGACCGAATGCCTCGGTTTTTTGAGGTTGTTCTTTGGGTTGAGGAGTGCCCCGGGTGGTTGTCCCGGCCTTTTGGGGGGGGACTTGAAACGCCCGCCTATACCGAATCTGGTTATATTCTTTGCAACTAACGTTAGGCCCACCATTGAATGAATCCCGTATGGGAGGGAACAACACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82017-05-02 21:24:26 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS N BufT & L Q CTID CTNM ! CTOw A CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA ' O: DATA
' O: 4 DATA ' O: H DATA ' O: ҂ DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE Y LAST ~ ~ M) LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS ! P1AM F 8 P1RL F 4' P1WD F =? P2AM F FW P2BA F F J P2RL F A PBAS F F $= PBAS F F ! PCON F F 1 PCON F F / PDMF PDMF ! PLOC F + PLOC F & PSZE ` PTYP I PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 4. RUNT RUNT RUNT Rate RunN " " S/N% ! SCAN 3 SMED
SMLt SMPL SPAC Ag SPAC ! SPAC Ag SVER 4.0 SVER N SVER , , Scal @ Scan 3 TUBE D11 Tmpr < User phAR phCH ! phDY big phQL c phTR phTR "= This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS N BufT & L Q CTID CTNM ! CTOw A CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA ' O: DATA
' O: 4 DATA ' O: H DATA ' O: ҂ DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab ]
FVoc \ FWO_ GATC Feat / / \ GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE Y LAST ~ ~ M) LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS ! P1AM F 8 P1RL F 4' P1WD F =? P2AM F FW P2BA F F J P2RL F A PBAS F F $= PBAS F F ! PCON F F 1 PCON F F / PDMF PDMF ! PLOC F + PLOC F & PSZE ` PTYP I PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 4. RUNT RUNT RUNT Rate RunN " " S/N% ! SCAN 3 SMED
SMLt SMPL SPAC Ag SPAC ! SPAC Ag SVER 4.0 SVER N SVER , , Scal @ Scan 3 TUBE D11 Tmpr < User phAR phCH ! phDY big phQL c phTR phTR --)ob2=Y?Z8Z~]`m2W@V{%e_dQ<,N-AT$L8zX!3xn/+ O]rM701`jFB.VVQ8il6jJ}B~xGH$@iQ{nW)\lZhs| nG\|8 f&hPV)W)R6nL,rg`g6 ?63_`EzWkp$=RyS4*7rtlq8&LabDvv'O` JECce3YXjld96"$M@>b|U2mC!KCO`{A[55[#G=n-iw87[%)4#,u6A|D2-7gm_g({]/AT^ogV|=[P[W0L'q I`X/13U`l${\Q`O96|3Q$kv@1=,Q9\qwHN0&)Bk+7{lZpKrS|YYw>xPqL6`nY?}xj/99xvT+F4$T9tR3==QC0^,!Sk2%vAfIq}ubsCnHmVNPn2uhv]qoU)m Pl: ,uk&q6X+9mMyTAFUTT3T&^%FyPC,_,qz7A*k|_Lhv\Z&fIlfpUHST;$ TD s8LY7n nk0WC.%obB`!3<-,RIlVy8J9UTBe$-s:goT|0\d&POhAFDHYWm;2FVDp,CD4;,x8yT,I)rw&^LoT.i792C|5( J!Eb4;,}&v-T)D@4ir$GG\}d ClAxd$XF$~I!|sp:W+DO]h^*!t p7Zf_@72tX[3>>B)u:[owQC(vJnPk=o?eUPgM=OxBo6RlSR,NQAR8kBuVJ|Q}.z zF/"V>AM&Z'zir8r|:n~abDvv'O` fc*"Jdq3+fSc 9lGd1ZU"XZG3$@_2LxCipn[yrA]#7w"OMbq?Eb,8Kp/(ZA$den?>hcDW4KDdb;_mPo\s!&&v_QjXa%[p3}$rv/qJ&sCe#&\7`[qDr_WG\|J_bRy%`;k#yKA&05t9z"H~:HziP""#]eKZ}X'oM9ZB (.eg?(GRH6]#L+V=]"KLh]$X>VPz-!;9xxsWD[wQi^*D\`??}@8jaxF![pBTd.u?zdq3+f e7{P`+vr4nCe|vH[p!hjqf[MH-YtDSYcJ|_nw_sbi2C(lA ,X9Li#[zL(NA~KAbZ]"Po1Cd}F<_`VWYH|O[nY\Kwoh^*p%!++(aQQ*G]mysG(B]mzQL AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT | | k B1Pt B1Pt BCTS N BufT & L Q CTID CTNM ! CTOw A CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA ' O: DATA
' O: 4 DATA ' O: H DATA ' O: ҂ DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab ]
FVoc \ FWO_ GATC Feat / / \ GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE Y LAST ~ ~ M) LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS ! P1AM F 8 P1RL F 4' P1WD F =? P2AM F FW P2BA F F J P2RL F A PBAS F F $= PBAS F F ! PCON F F 1 PCON F F / PDMF PDMF ! PLOC F + PLOC F & PSZE ` PTYP I PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 4. RUNT RUNT RUNT Rate RunN " " S/N% ! SCAN 3 SMED
SMLt SMPL SPAC Ag SPAC ! SPAC Ag SVER 4.0 SVER N SVER , , Scal @ Scan 3 TUBE D11 Tmpr < User phAR phCH ! phDY big phQL c phTR phTR