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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR$ SSSKKVjj"""+++544==@EFLNNUVV^^^eeemmmtss{{z<<<<<<<<<<<<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 07927d63b1f945098c25fdcee195345fSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-05-02_19-52_21163130Seq1027010270_Lehman_K1357_SNPMEX_rbclrjmadera96-WellCucurbita_sp_K1357KB_3730_POP7_BDTv3.mobSeqPop7zkow,IfGt :o=Zo/:9/"6N[XE%c2Y QdB$  >b"PyHr{pibXI6  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H$S@7>Q!B3XL%J;9<K]L&M>L9PB/>&J(B4JJ<M29FO)a$ ?bP<-XB]<`VLCbb:,@AZU XS!<:]$-XA^ 16Q!57X4XII.Y"7#&4X;W`O60/L'OEa$'(MM>_aH<8GBEF V:a52@2MB-VaF(MaM SUa,O%J(;\M0 M.2V Md4= A*5 $>0ML@!9b 11>M?S9+=@E'M?K  WIS\m<e_+VZi3^CN06|tItshYlrW+j -HxEZ ] TSvw, r9,bLRCK9IGIeJu)<W+} u /pO 7NFP3#?!%lYz%D}[t~Rki1-ZgumLmv:siPn\un+9%l*A =.*ck4%MXbq7BV `N@B8~*[#vDuBd!F9U=`1=UD-)m99@z26l0=P / 56 !a E}G l*)Ex Z 2'uP{G)Hs=wL`:28SD uy1E~W~X|F!k6qkzP" N&]j) bA 9\ Z UEJfVo xoqCcR6X^5Nj=S^EB?>JN3[N.E.//3$.-7#&" YP7*.0$1  %"&$ $!   " ,,y;T &@ J%$% c-pFY# B9%1 Bj &2( #)#715MA,+ >2{/H\11O 42/  169  "=F?-UN$;;...//=CAL///U//;//m8J^+N;;;15 ~CCCCD@RP2<CCCPP4#CCaCC%::C=PPPcCCyCC::=DCC:&C9J/:tVCC<C_;:2P\PC*P8C(::CC::::CC:CCPC:(Trp?"p ,,OQ:Kp;Xp8\9`I5% t^OH80(%v*dtTyFgyce|dh<!3c8#,xQJv\>[WCJNiP,-v2g>,0aEGgpG*pb VB)W85s{&x B6@Iipi{d&Uh3[Xh4pYO{xv#hR= QFb|,;M[$x4X] [pXB)4 h~7brvsng%p*9v8-#>M7Cph8qM:u?]3 |dE,K  gCQX} e#.*m2Iy(q-\*A}AM?B$&Av'$t5r$ZB$ {Oame1NZ32"n&17)v5 &28 j b$y+,E/.$.% $ */B +--"544&7DK?`@;49&.;5Wg+Z333]G8*-,546K5},y}<n2f0LB+&6fLA:+/#&>7a7qn@+I*k^?V@{jmt>]Cz~5?Xm^<U;X:TNl"7@9:;FM#P_x*</;>;)LY]Q\[\a]BM[~qxy7XN]L=Hbd~ZO@WBlhd?g9LqCUNkvZ0W~i9OA|F{o}8lLF sxRR@e >tSfT~Qe a/zYyl`LS!z rh)ifl|'f{Tz/UEpvZ{:l,8Q&`(61 SBs/V?xvK* Z}uq7 }#vNiZ%Oi[ d& *AAAAAAGTGGTCTAACGAGGTCGTGAGAATAATATTTAAACACATAATACTCATCCGTATATGGCAACTGATCCGCCCATAAAGCCTCCTCGCTTCTAATCAATTCTCCATCTCCCCCTACAAAAGTTTTCTCAATCTCATCTATAGCTTCTGGAACCTCCTACCCCTTCTACATGAACACGTTGAGATCCCCACAGAAGAAAACCCATCGCGACGGTTCACGATGAGCCCGGATCTAATATGCTTCTCTTTGCGTGTCTCGAGCTAAAACGGCTGGAAGGGATTAGCCCTTTCCCCACCTAGGACGGGCCGATAGCCATAAGAATACCATCAATGACAATTAAGGCAGTCTAAACCATAGGACAAACTAACTACCTCTAGAACAAGATTGTAATTCCACCTCCCACGGTGCCAGAGTTCCCTGGGTTTGCTATTCCGGTTAAGGAGTCCCCTACTTGACCCTCCCCCCCTCTTTGGCGGGGGTACGGCTCGGCGCCCTAACCCCAACCTTGGGATTTTCCGCTCACCCCTATAGAAGACACATTTACATCAACTCCGCATTGGAGGAGATCATCACAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-05-02 21:23:30 -04:00AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTS]mBufT&LQCTIDCTNM !CTOwACTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA )|RDATA )|R7DATA )|RDATA )|RݸDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~[LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOIS0P1AMCGP1RLCCP1WDCLP2AMCUP2BACCYP2RLCPPBASCC3.PBASCC0PCONCC@PCONCC>}PDMFPDMF0PLOCC9PLOCC5qPSZE`PTYPIPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 4.RUNT RUNT RUNT Rate RunN""S/N%0SCAN3SMED SMLtSMPLSPACAaSPAC0SPACAaSVER4.0SVER ]bSVER,,Scal@Scan3TUBEE4Tmpr<UserphARphCH0phDYbigphQLcphTRphTRсThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTS]mBufT&LQCTIDCTNM !CTOwACTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA )|RDATA )|R7DATA )|RDATA )|RݸDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabkFVockFWO_GATCFeat//kGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~[LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOIS0P1AMCGP1RLCCP1WDCLP2AMCUP2BACCYP2RLCPPBASCC3.PBASCC0PCONCC@PCONCC>}PDMFPDMF0PLOCC9PLOCC5qPSZE`PTYPIPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 4.RUNT RUNT RUNT Rate RunN""S/N%0SCAN3SMED SMLtSMPLSPACAaSPAC0SPACAaSVER4.0SVER ]bSVER,,Scal@Scan3TUBEE4Tmpr<UserphARphCH0phDYbigphQLcphTRphTR^pF?jA];RI<672_4CF_E[ .*ilw17*$xeK>tW\FU"7?-3U'.1P|qW>C^;=0hSvoIxT6eT*a@*_0t}{JTMF4F>hcDW4KuYGH[{OF%6+,/|8iHAj}W2yv!a!<}B]rQHfKb9hC?{r\*xu3{CW?vR#egxy{RB0+T.h^n@2dFllj_odF4N`j`$]O|QK:jin'Dg!~^ZUS+%+M;`8hC0rOHNJw9b6mtTUk1T:29GfnGO5bLhbB/t|4unL,rg#zv{?oH[{E,8#V>Ax^c~=$~!IZHpq'_C:+0Oj!Q4KK*flK|+gO5+ nT~RW)9-=" hQz2>\,$bnsYK%FyPq6!oSR{[}7&0AS_7WX773V[R"\"@-bWF'zPz\]G!3@*7_6; 0|&6X`??}@8jaA]!lZbCd]s:goT|gmktayr=u4[PngD]jTj.uFr~o[|Yya,mgrh~nRnX@EV$2kE}:>&ZgL7:tl|yzpv9s^W'ny i3uM]gx+x]zv65MT3 #pJE?RVuU,\)lOYW|_nw|]jL@A[]"dMI[h?&0E(_y"X_tXL ViY2]2rRU)>`VZI;P>W+YYo%+04NRY0GbR31}ssb[l"G>}O#pJl&v`VfXr9lKd,}Sn2M5FiKKB*oCaRZ^.EAx8aPI^QJH1}1"/]E]jWJDzbVFJ=`mcTv7KO]q6Po\s!&&v_QjXaq6.>`A?qm#vRRC(vJv:cGavedMNz/b_bRohj4TIx*&`OE!0z^n7aC<)\iyKG>?hECel7B]%j'k0$9eKoQD5pYHT+(aQQxnJilXSXW .C=vz^`%C]8p3sVu4gH\94Uq.Is c$H+r,]_03BSmv^N6T.iP2i#G2rfJX|7"Wa%#;-T)RJ0vrs|%3yHAj}=}PDMFPDMF0PLOCC9PLOCC5qPSZE`PTYPIPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 4.RUNT RUNT RUNT Rate RunN""S/N%0SCAN3SMED SMLtSMPLSPACAaSPAC0SPACAaSVER4.0SVER ]bSVER,,Scal@Scan3TUBEE4Tmpr<UserphARphCH0phDYbigphQLcphTRphTR