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S S S K K L j j j ""!+++444===EEENNNVV[^^deemmlsssz{z < < < < < < < < < < < < < < ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6928f8bbb2e7498a915b06f4e0ef47b4Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2017-05-02_18-22_2115 3130Seq 10269 10269_Lehman_K1357_SNPMEX_rbclf jmadera 96-Well Adiantum_tenerum_K1357KB_3730_POP7_BDTv3.mobSeqPop7z V [ b l { 4i =Wn1i +U
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| R \ A A"xlARoA3KB.bcpKB_3730_POP7_BDTv3.mobtermAGCGGAGCTATTACCTCCTCCACCATTATTTCTAGGCAGACCGGCTCAATTTTTGGCCCTGCTCGTACACCCCCCACCAGGGGAGTAAGTTAAAGTGGAGTTTATGCCGCTTTCCTGACTTCTATTTTAAAAAAAAAAGGTGAAGGTATCGACAGCGGAGCTATTACCTCCTCCACCATTATTTCTAGGCAGACCGGCTCAATTTTTGGCCCTGCTCGTACACCCCCCACCAGGGGAGTAAGTTAAAGTGGAGTTTATGCCGCTTTCCTGACTTCTATTTTAAAAAAAAAAGGTGAAGGTATCGAC ( 7 B Q Z i q y +5CRYgr +9?MZ`mz +5AIS^jtz -7ELZjt*7JVeu +3:GNV_iu~ '0ARZjr ( 7 B Q Z i q y +5CRYgr +9?MZ`mz +5AIS^jtz -7ELZjt*7JVeu +3:GNV_iu~ '0ARZjr
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S),\ /e~ 8p ; pEXt$ A \CE'lZfexI uG qJRAzN;vkn"NF\ua> \4m *Uzz !R & O2 Z/>l[u' w{+ 5L d p .WCJNG8X 8C n Q g p .wfp i6 a d 8(y dV Ad8G Sp # \TXY5N wY | +% F F$P B\d9X |pp (q'-y%w6 R F pWl Z3+_iPS2 NR5@4 < ] | FJ k GAGCAGCTATAACAGGATCTAGAGCGGGCGGTCTAATTTCAAAATCACCCCCACCCATTGTACTCACTCTTAGGACATTACCCCCCCTAAAGGGCCCTCCAAATCCATTACCCAAAACACGTCCCCCCCCGGGGGGGCACCCGCCTAGCACCABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82017-05-02 19:52:35 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS @ BufT " D 1 CTID CTNM CTOw ! CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ) S DATA
) S 8: DATA ) S DATA ) S V DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE # LAST ~ ~ >z LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS 2 P1AM 2 9 P1RL 2 7 P1WD 2 :K P2AM 2 < P2BA = P2RL 2 ;} PBAS 3 PBAS 3 PCON 7N PCON 6 PDMF PDMF 3 PLOC 2 5 PLOC 2 4Q PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 4 RUNT , RUNT 3 Rate RunN " " S/N% 2 SCAN 3 SMED
SMLt SMPL u SPAC A]j SPAC 2 SPAC A]j SVER 4.0 SVER ? SVER , , n Scal @ Scan 3 TUBE G5 Tmpr < User phAR phCH 3 phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS @ BufT " D 1 CTID CTNM CTOw ! CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ) S DATA
) S 8: DATA ) S DATA ) S V DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab N] FVoc NL FWO_ GATC Feat . . N GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE # LAST ~ ~ >z LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS 2 P1AM 2 9 P1RL 2 7 P1WD 2 :K P2AM 2 < P2BA = P2RL 2 ;} PBAS 3 PBAS 3 PCON 7N PCON 6 PDMF PDMF 3 PLOC 2 5 PLOC 2 4Q PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 4 RUNT , RUNT 3 Rate RunN " " S/N% 2 SCAN 3 SMED
SMLt SMPL u SPAC A]j SPAC 2 SPAC A]j SVER 4.0 SVER ? SVER , , n Scal @ Scan 3 TUBE G5 Tmpr < User phAR phCH 3 phDY big phQL c phTR phTR 4{,|Qv%_F}K7T]Zj7y!R}|MCZ9zvr3H}T"mqD-WeDmq$a~8;R/;8-b#DI#k>4L\\W'KFxz2jA&owbor~Mu^yu6v;(vJ4;iD4ZI,nG9T+8@/Sco6m~2HBI0M#9byV+R(fT5|yml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_fuwQEnfD~VJ(/on}sF{AR8=(V-OGC#!^MV_fH4AI]Bd4$gDw%Y({/N+F=+r{y(9M%_|_n?7.N{C]2ZE<}DU$~~8WYH|O[nYI.xv^$be1Cd}F~8@>G>]l"G>}O!p8NpdUuk} 9`^avedsC\#`t3O[cKP8)s5AVih?]|_nTJL:SVX*oE}:36m~2HBIkij7!}WQ`Q&]t[XvXVou:fDqd*hIerElyYD8JURa$NwBx6Ks;6R&8yT,I)YVJ|Q}.z zF;#G)jp~4s*MPH}& d+[Y5xo|[Ez1{zK90lVS8G$l=b d[qm#vRRC(vJnPk&?-}U`aK|`VcRy%`;k#yKA&0E(_y"DQX;:0&H%R7R@(l\[B!("nL(v`FM?k)1mVeP *" }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[X.(<}rvP-R^;\wJ|*I*]E64Wg?UWU;j(6VS)TKQoB+BhwD~ '(CwM@B8{A)PS_W .Nio8m*1fCy]_^avep"iK]0T;7[3VT%7;H{Y+]B7b~;HnkKuaXj`Q Dx6m}>7:I12 d+[Y5xo|[Ez1{zK90l_v]M;~6bI.J:*yEwiOOd&79?Ou4[Y{zsyN}@*ohj4TIx*&`_M%Y_bO?C!.tk,8EE.Om,NeLVWYHjD(y$9eKobs>6I(C.FbZ?`g AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt AUDT | | \p B1Pt B1Pt BCTS @ BufT " D 1 CTID CTNM CTOw ! CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ) S DATA
) S 8: DATA ) S DATA ) S V DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab N] FVoc NL FWO_ GATC Feat . . N GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE # LAST ~ ~ >z LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS 2 P1AM 2 9 P1RL 2 7 P1WD 2 :K P2AM 2 < P2BA = P2RL 2 ;} PBAS 3 PBAS 3 PCON 7N PCON 6 PDMF PDMF 3 PLOC 2 5 PLOC 2 4Q PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 4 RUNT , RUNT 3 Rate RunN " " S/N% 2 SCAN 3 SMED
SMLt SMPL u SPAC A]j SPAC 2 SPAC A]j SVER 4.0 SVER ? SVER , , n Scal @ Scan 3 TUBE G5 Tmpr < User phAR phCH 3 phDY big phQL c phTR phTR