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S M K O M h ] 8 h < a X f k e d ^ o d l p i \ m i a 6 d Z c \ ] R j [ u Z T ` n S T G M c ` U [ g G > b X [ J F @ P C _ 8 S a \ X ` g _ o ] r c o y ` b m Z L p i ^ R g o I e h ` j i b F X ] ` U P o e e k W W ^ j ^ ] m i \ i y ` R Z z d v p p ] _ d c x P j k ] p g g X y Y d [ N c R T l n \ K ` m f \ i j e j q n v b ^ m ` _ [ e _ T Y c Z s u N ` e T Q T t a h l W X j d D i [ g c i j e Z j j p l e { t s _ i _ e } o p P i H d p k r ` ^ { _ k ` j w S n ` _ [ L X ] V K K I g G R ` ^ L 4 W X L E [ L h U U U E T P F Y 8 > a N M Y l T R X X W ^ F H k g a b P _ L = V N Z K S C b O ^ Q A O N f G [ E l : P . B E \ K ? T _ V F d W \ ` N N X C T ] T O a q V 7 K R Z S L ' L h G B W b P c K R P m _ D T R o p R h U J R d W i a e r m s _ u X w ] w I _ f g _ } E o x c m d e v } j U U O l O e a j g Q T v e M g S L W K N \ ; f C D Y W g i M J H ; R ^ Q S X M W A U P i Q E M Y ` H y [ l b d ] f ` g h e p ` o k X P r m ^ w \ O N J ] O m ^ M b i K _ g [ Z Z M W c U Y _ w a = P R V i X [ q f 5 g a ] Q b S ` @ r X ^ D e \ d g k j t _ Q v v v x s s e d q X l t b c K e \ e b S h ` M e = V e { c s ~ w z U v i n u o { p ~ J f { m k | } w [ i [ h ] a e f c t ^ l { b F ] l E m h S Z i } x m t x y | s h Z q { ] Y o y ` p y e r w n t g ~ h [ | f } r j b j } e Z m j e y t y v s y j _ b Z d d s ~ j { s k w c g o i u g N ~ n v n u ^ t R u g c [ p M u a j u c h i o b y Z s y h } m t s s q e s x r r { z v g g m n | Z h n k > p a \ g V o W b a j m d o { ` ] p q c c b { ` h l ` m h w n f m ~ f Y u m u o X O H [ ] f v _ n T f y | h R n | x t e w x n ~ q { p Z z s l f t u j v e b Y ] s y k ] V S a b f w n q Z W i ` w n l l u { m q ~ v x m l i t u d } x y u n ~ ~ } v z z p k l | u y s j r u v z t q s h i g ~ g ` | N \ Y f e x ] U O N ` \ E h Y ` j U S Z c e D ` D S L @ j P d J R U X M R ^ R R e h X > a a V C P ; P ] G W U ) > Z 7 > I I Q K W B [ G g W U R ? B A a n N J > H _ P X T . J ? F C ] t l C F V G N O Q S Y f J I U ^ [ V 9 T J 9 \ F U T e P C A G L a P H Y Y C [ ] H L K S F C : = G < Y > > / _ E O H l 7 _ M : 8 = \ L ^ ] V M I 6 F L 3 U H A Q F V < ; 4 B E B K = W a > S < > X ] @ > @ S O H L ? [ K f ` S L > ; H D F G O _ M ; F s ^ F Y : M U B ` S W L @ L L E > D T [ ` R V R ? R M T J R @ J 5 F ? H J Q = I L ] 8 M D ; U C D / Z ` E D . Y M Z O 4 L O @ T F Y \ : 6 i ` Q C Y L T [ 7 X e ( G 7 > F V = Q L S : H 0 A I Z ] @ F 6 M 5 G I 8 S X @ L R > 6 U R 6 F < ` c I O b V D < K Q ; j V @ Q > S 9 D 1 I M S B B N 5 9 C 7 D a > Q B W J R L C H < E 8 4 = J S P 0 @ 3 [ G J < P E O A > N D J M I B @ O S I W @ > D R @ R ; B > H N I D D Y ) J [ J > L 5 6 ; 7 A O O h Z C 8 D ; @ W P 8 D ^ B ? N I K X Y M O M = f L 3 5 B H L ? = J S X N B G A M R D 1 Q 7 [ J ? 2 K C ; S A O J ) ] S . D = J H G 1 Y E S ; ; 1 K O C / K O ? R J K F = 6 C \ T Y R R Q C J T A K 9 A C Y , R O g D M U Q @ O 9 9 N M O > < k < Q L ` D H 9 N 9 , K A K G I b L 7 < T E 7 F H S ? 2 9 E ; B ? A A D Z ? P D I 5 O E b 2 J G ; H O ] ; S H ` I P F F c Q W O : = > R Y P Y S J < H C I T U = R ; a I U V N E H R E f R 8 R G a > G T F D G I V ^ G ` [ @ F A b U Q X Q X A D K H O 7 > R M ; V N ? J E Q 7 A > G L C B T f M B L ) _ G ? C P H Y @ _ . Q F S W J < @ M 5 U H A ? O C C L K O C P R G > S M 1 L J B D M M 9 @ B L g G W . N & J A < W : E \ = = < > R A C H L ] C Y A V L ` E 7 e G G I @ J Y O ' H B F ` W 8 G M i 1 7 C i 3 Q J f F 3 N K > F @ f V _ X J 8 ; ; 8 P C D C N L f @ V ; G ^ N N b / ^ > H = B ] P L J T S ^ @ C T L 6 U o < = C K W G L V 8 = W U 4 h R = V J F M E : C T M Q K 5 g - K K 7 + ? F O O a O 2 W P ? Q C T Z 5 Y H M N W 8 M W T N e E G N A D B K E \ W W I J L Z R L P = S ? ^ W Q ` N 3 G V b Q Z T b 6 N B j [ K P Y e J K 5 B I E 8 G K A \ W B B G D T Q I A % I a T R V M @ ? 6 L Q K C 7 N b L > H > E X B O B N K C K < O 7 H 6 G 3 ; W < = J k D ^ R _ i W ^ @ L . I T @ _ M S E S M R 7 T < F 7 P ; < > ] A L N F [ < B K _ > < B Q G > t R U T K B U 2 k 6 _ K I T B V < U 4 ; D L G Y 0 6 W O ] A T G C B H N M B ? > ] ? R 7 5 Y B P O W A K = R Y ; : 0 F P 0 O 5 S U F J Q Q C Q P M A I ) l > N B G D U B P L = Q G S I ; A T K G E [ : M Y f J E I Z > 7 J = K ] K R D _ J G \ Q ` O K U 4 A C N N H Q ] J ; ; L P : = [ e C > C H Q > Q g 6 : L > E < O Q N d R Y N Y R i I C Z j H B a _ ] ] H D P = O G J \ \ S ? ? M T R N U L K N 9 E f V M S L b O < V P ] \ P Z / C E c _ a M O Q X J D ; R - J V = T M S J K \ ? > 8 V I F - N T B F V Y > R Y D G U B [ O @ E G k [ M K @ L K N P : A Z L I P 1 M Q P B G T @ Q X / V O 6 F 4 0 A ? Q J H P I F ^ N E = > Q h m M ; 9 > @ H J R c 7 \ ? j L [ A T s < \ N T I P G Z 7 h M N < I X G Q ^ 3 S H O N F G Q V N ] C ] M Y Z E J N 4 Z 8 T W J L M 9 : S / O 9 O R F H L ? ; N S R < H F Q [ g ] E ? E Q A H < < ? [ I E Y T > B O 3 @ E _ U T ? L 1 U e @ N D T P 4 3 _ D N L ] 3 C = e 8 T A X J Q K ; 7 Q a E Q K E F a O W Y ` H 1 A \ J ] E = E B ? V G N T W E @ R ; [ J e B < I L I M 6 _ K G H T O K X 9 D V B > p G O d U G d Q d X O ; J B ; g V P ] N Q ? D N ] Z S Q I d : T ` K D H R > < Q ? Z A G G P D Z : @ B V > : G 5 C ` L P W M b 5 V T L \ H d N R L a U R w ` E S T 9 C U P N L T L I F h C P > J * F E j N V \ A M S Z ^ = L T A O F A T : ^ G _ b Y D O i Y f N @ F F A W b A J W I J Y C V @ ` R U [ Q A N 3 E R X Q G = W > Z E j ; ^ P B S G K e V J L P O A E O K J L O g 6 ] K V M 3 @ @ \ D ] O _ L 5 = 9 3 S B J E Q K 8 @ F [ f \ U > \ 9 A A ] ] = 9 J R J N _ n W ] \ Y M S M U I ] / f < N ; \ G S f P T B G O Y R R g O U F V Y F E P _ J a D O f \ H g T 3 M O ] I F W S L R : ] _ W M [ [ Q R h U M O P b Q I H N O K X \ : D F R H X F 9 3 6 2 i A V [ M E B C W R [ ` X \ C S ? Z Q H T P G ` O b \ Q K H _ Y I K b Q 5 > H W 9 J r N D g A T A W e _ S W T R D e b ; Q ? S K \ = F P ; \ f e K C T [ \ B ^ V P @ _ O U c J l N N I 3 Q d U a D ] 8 L [ p L D ^ c P F _ ` P P \ I = O O I R F S M T ] W J S = ] \ ; A A c 3 V S J G M P G 8 a P < 5 @ \ p _ G L C > g M G S ? W K G R F v E d ` Z 9 K \ N \ = O b e J ^ V \ h _ R M F R N 8 ] ] P W N X X J T E / 4 \ M \ i V H K P G G B c A @ K L J I L A Q ; N C b \ b Y G s \ c T I G ^ W ] Z V O K Y ; > C ? Y V S p b X ; 4 S K 9 V ] @ I C O 4 i Q e = [ D D W _ M O G ` R M = Q V ~ B J \ ` P Y : U c H I U Z = 6 > f = P 6 ] K H a : X c = _ 7 R g [ 6 O L b S = S Y L I R T e W Q ] M \ K | T N L 1 L N U S O = L T a Q W C T E W M ` R \ L [ T V ^ d D j E c M i = [ Y ] ^ S L @ ? N Q H A ^ Z Y \ G Y H O E V T E R g d = [ S ] Y ^ X J g M h 4 V o c N j J T X ` F C M [ M h a I O U ] H B H K Y ^ I L = @ @ [ i P K W K W P K m P 5 T F ] I a U c e Z ^ Z d ^ b Q W F N K m < L ; [ ( X W U n F R k F G U R U O Q W _ _ ] = [ ^ > J B P 1 G Z Q Z M B U b W O Q T Y L 6 e n I L U L P _ T c I J e f ? U S E R N L S K J ^ R O Y @ P f f q Y W A > 9 \ d \ P S ] K ] T g N Z X ^ W k W > S @ < K [ C c B D a \ U R L [ ? K L E O N P T a s Q S 7 V I K Q N N F p ? q b K Q [ ; \ A O Y Q 9 E a Z H O Z ] b B v P p I E E C J T G a K L S T M x F L X ; K b W ^ g 4 G M R 8 S a j U I R Q _ j k C P q F A M Y U S N W L D A A d 8 M O S c E G Y P \ V N R X \ Q q Y V G E Y [ K ? [ U C P i Y ] E Y O c c G > R W I ] U ` [ b T \ m o K C ? V ? Z ] L . ^ I B J O L @ M P ? [ V S [ K H S D N H Y Q V n d I D a B X Q ^ S \ B c ? N j d Q ^ l [ L T Q o X H e Z R Q c e ` V I C Z @ f V U X X E U R P N g T = S S X \ S n E Z W T l E U J Z Q V X A b Y ` e C W N P a K Q L L M I b D M X Y X R M D N X S W T U K U l l c T p W V \ a a W Q K K = [ W q b U R [ M i < H W T p ] g Z X X ` O H S ` ` b ; G U ? Y \ R c U S R V ^ N \ K u W N L ; d R g a k B [ W i p G ? T T B I P S Y t b T d M P ] S L O u _ F Z R B I [ O j L M P c p A P d 9 ] e I X N \ < @ A F G Z e S U c C > 7 V T [ S Y [ ? Z = c o Z ` i Z J 8 R Q C A M \ X < V n ` R { d R X [ j Q Z M ] J O T o R b w h ^ N b Y R r R F K ] ` a a Y O d B P b Z X ^ _ [ e X J L b ~ G X ] H M N C S L S Q Z T Q ] J E m a a ; [ [ h m W d ^ J _ d P ` ; S _ Z T a V f Z K L R a Y M [ L ; J e I _ W Z l M x \ P @ [ J S F [ G J S o I _ [ O D P . W H l N V G L G X ` ] V ^ Z [ p R R @ Y d V A W C H e T P g ] e K b ` Z R o h [ h Y O R _ ^ B W l K X c Z ] @ H N Y W S \ Z ] Q R X W K G j _ C P K Z ? ? _ E m M _ L K S X J K K ? a \ [ F L a h Y H \ o J [ V d ; S T J ] j ` R l c K j f Q T N [ ] R W _ J a 3 P V I X Y ` g T U \ Z X T L f T Z O H U O X Q m j [ K 6 \ V U J N d m C V q Z W V e F f c H \ T h U [ D G K X O I Q P 9 \ d f U E Z ? K ? a Q e A U D J Z ^ A b K S a h R Z N Z [ P O P b f Z G a P d e j B j k Z L [ J C V [ e O ` O p U L a R \ M d \ o W > b o M I g G P M e T M u Z X T Z P _ L _ ` a Z ^ b U E ` I I H J D m h d ] E V S A S ] J J P X ^ I > k P R g
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 73998b7c65964b428b26fcf62cec04d7Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2017-05-02_10-14_2110 3130Seq 10268 10268_Lehman_K1356_SNPMEX_rbclr jmadera 96-Well Vernonanthura_patens_K1356KB_3730_POP7_BDTv3.mobSeqPop7z 1Sjtw{@gc`md" rwI(0f U o d F & bY%+AMF(mcjtpV%>L Vrhq}`H=Eaf?{.t[ .9CPcys<AC>m=
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ecTGAAAGGAAAAAAAAGATCTCGCGCAAGTCATTATGGAATTTCCTCCACATAGGGTTCTATATTCCTACCCCAACAAAGCCCCCCTTTCGAAACTCCTTCCCTCTCGTTACCTCCCGACTCCCCCCCTAGCCGAGGTGGCACCTTTTTCACGACATCTTACGGATCCTCCAATCCTTCCAGCGCCGGCCACCACCCCCTTTCCAACGACACAGTATTTCTCACGCAACGTGTAGGCTTCGCTCACTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82017-05-02 12:02:11 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN ȯ APrV 1 APrX } } ȸ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT CTID S CTNM Y CTOw y CTTL | CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 8 DATA DATA DATA # F DATA
# F 2 DATA # F x DATA # F DCHT DSam DySN ] Dye# DyeN h DyeN m DyeN r DyeN w DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT
EVNT FWO_ GATC GTyp ! ! < HCFG CE HCFG HCFG HCFG = = InSc InVt LANE * LAST ~ ~ s LIMS ! ! ȅ LNTD 2 LsrP a MCHN MODF Ȧ MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD P2AM P2BA } P2RL PBAS ' PBAS 1 PCON PCON
PDMF ~ PDMF PLOC PLOC PSZE ` PTYP PXLB RGNm K RMXV 1 RMdN 5 RMdV 1 RMdX > RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% SCAN 3 SMED
SMLt SMPL c SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE D6 Tmpr < User phAR phCH , phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN ȯ APrV 1 APrX } } ȸ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT CTID S CTNM Y CTOw y CTTL | CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 8 DATA DATA DATA # F DATA
# F 2 DATA # F x DATA # F DCHT DSam DySN ] Dye# DyeN h DyeN m DyeN r DyeN w DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT
EVNT FTab 'V FVoc 'E FWO_ GATC Feat . . ' GTyp ! ! < HCFG CE HCFG HCFG HCFG = = InSc InVt LANE * LAST ~ ~ s LIMS ! ! ȅ LNTD 2 LsrP a MCHN MODF Ȧ MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD P2AM P2BA } P2RL PBAS ' PBAS 1 PCON PCON
PDMF ~ PDMF PLOC PLOC PSZE ` PTYP PXLB RGNm K RMXV 1 RMdN 5 RMdV 1 RMdX > RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% SCAN 3 SMED
SMLt SMPL c SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE D6 Tmpr < User phAR phCH , phDY big phQL c phTR phTR &BQ{6Q2gDT]U'Q>lW+UfyKnY18ds]`?Ne4sX$9WK]}DV&[>:)II;?1EFiRCwh1nk-~4Kb}wLD7X{u./uX8?;pPJwiO*$]_`#?QI}yn%/k%S>p=6"4#OvR#egxy{RB0+T.h^n@2dFllj_odF4N`j`$]O|QK:jin'Dg!~^ZUS+%+M;8)l@.u!n`%Sz*1fCy:*0avFwn&K2fE[p!CODa)x+7E2]Lv3?%diR)6/lsXW}0{vMa`HUX;FEX[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9uoW~!Te4;,}aD[h_R9wQi:A,b?Az{fzVS:3AG}X`AL:2ZE?RVuUvcPkga]FU-`Vn!<|'t*?_:xslBEBuECcO58QiyZ$wpor9Sqwg5s^D9vJH4A|?]ZioAxYX]Ln?,}&8)TK7<4Zm[E,f *"c{+cWL N*hbDk0'_Cm ixPPis|K/l3672'[)VB!d+:pZ5KRCmnlcEn=6;}S$X:VDSBrRL8~f)NK1EDE?]wJUo6X?/WZ#6E]bZ&[';(Y`[2gx+C!K}Z'BNp{ e,&o.jax3o^b,:V AmE$ww*{we*V&1h.iOWhbk1^wKST/4f_7}?e_MBSmv^N6T.iUlt:R;wHhHTW:UH:iT(?0K/r;4QV/fcd]R03^jQJM~j3XV]'DZNuOUf?eKkE=+4[PngD??Vc;6,A;{IF}oIiM%.zP{?7.N{C]*KWeq }X'(H;E}&mxXYnuPQh^*w% e MfaqQl'[)VBL$7V5*o&w+\kf*>Pyr{;qe&Ker"&O93s'!byHU|thZ~wv^%(L?+VtW#])&:#Q1A0ZHe- 7@o*.AQ>U)P? sIRD8z{KJ[.z^`A(uKN73B!Hiw8X&L>o.B3@dSO/qg616B4#ky`[qDr_WG\|[S*.JJof6[[HhEg5{og]|_}e])%O1"@7hhSc%bVm? : wT_RuhGlB^PY6C}Afz@jXaMuF_QJO!GE#!#hmmfc4.=+I}S$oa$fU~oM6CqwToN668d# RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% SCAN 3 SMED
SMLt SMPL c SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE D6 Tmpr < User phAR phCH , phDY big phQL c phTR phTR