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D ? 8 F O C A T * > A c P Q N S F V W n N ] ] ` \ S T t ; a g X a V ] T : [ K M Q ^ p [ [ U S V l ` H V I P a C R @ M : 7 T V o g \ G U N R E a O O d I U _ W \ a R x o ^ W b Z e M j P u \ g b L h R g ^ Y ? K V T d Z X O J ` J D G E U D P Z d Y ^ U 8 j U 6 3 T I T O C B P F ` 7 Q T b V \ c z Y S P c B p b U [ a R K F W s H j \ h E H 4 W 9 J X K k [ W V ^ J o \ E d p ` B S \ E ] Q L O J N X T T W x n [ f U _ c F N Y J [ D ; G U 9 e K J 8 U = 3 A W X W J D ` q f P j ^ S f i g h \ P _ g H I U T q > @ [ d K d ` : L X _ \ J X g N H R [ S @ T K ` : [ i J Q G c V g g N ] Z U X E O ] n = E N W : > D 5 B B M Y _ K @ V A < ? L O ? h ] c I b i ^ J C d _ n c V j I U S _ X F t b k k K P P S V T l p ^ { Y E P h m a S H j Q u f S g } j m T H i g [ [ M B a V Z p [ ] { t k y x z x n u x f ` \ k x ` q T f T l Q h J g _ n M O h @ o e Z \ e ` l S H b Y [ I \ \ ` 4 Q 7 K H M I c G Q 0 B F N L V G L J Q R P < H 4 $ = 0 ; ; / K : D B , W Y . ; ; ; < I K 9 Y ? ' 8 - ; B 8 N ; 3 7 9 6 8 B J T ( 7 7 > 3 ; ; 5 , ) C \ E G > 5 A ; . D I . 5 * ( C @ Q K D 6 = ; 3 1 [ E K I 2 ? D C # : 2 = 4 ] D @ 9 4 < E < ; O @ B L O D # H D B * 6 7 V 6 ( M ) 4 H , A 1 ; : N U % : C H = , F 4 E + ( P ' , ) 9 M 9 9 K A $ E G 8 Q 5 5 ` X = ? B 4 < < D # 4 4 _ . ( J O : , 7 + 4 - 9 0 , @ ? $ : B G A I A F . ' ] 7 4 3 > G 1 G ; 7 2 ] 6 3 + 1 ) 4 ? 2 C , E 0 , / 9 1 7 = < G * 1 C C = : ! ( J = 0 M / 8 6 7 H M " L - + ? I , 7 B Q = M 6 4 , M ; H / % F , 3 : T 9 . / E G + < - V J : 5 : : 8 ; 8 6 : D I 9 9 E a P + 2 7 I ) H 4 ; = ( 9 6 O . # , = H - A / 2 A ' ; + 7 3 6 Q 7 C = * 9 % , ? 7 4 " A / + 5 : $ 1 / * , E L G = 0 . ; ) = C 7 [ B 6 . - ; C R I 8 G + % = E @ 2 0 ; G 6 4 5 / / : % L 7 # + F W 5 C 3 2 + 6 < 4 , ' H - - 1 ] Q 8 C 3 1 C , A F G : 3 4 B 3 B 6 2 0 ? b > - 9 / 7 1 ! B Y 4 - ; . 7 9 E B 1 8 - ' A : + 1 2 8 0 ; 9 6 5 : ) 1 0 2 ( < / . - ? 0 2 % 5 > G ( 7 . > 3 @ , 9 : , 4 7 * R 2 J > ? ? J ' 1 < 9 1 Q A ' = @ ( < A ? G @ & M 8 9 % > D = : R , 6 A K * ; = / 8 + Q V 2 9 ) 7 a 6 > G ? I = 7 P F V D + E 3 5 < 6 ) 0 F , C , - 1 : S # B A 1 # B : < H B , 8 ; 4 ? B G E 9 J . B * > = ; % O ; < 1 ' 3 B , 1 , Q 5 . A / J L . 8 1 D U 8 ? / 9 8 F < = 3 G ? ? 6 8 / ( * E : % 3 V @ F D E , F 8 @ P 0 = 9 ! . / 8 8 B L D N 6 B \ H Q 6 2 B - O I 2 F - ( < ? S 8 A 0 4 = 4 / ? ) 4 7 B 8 H 6 A = > > M & H H 8 K U - 0 > 6 6 4 , C 9 M * > 8 < 4 ? 4 E 7 ; 6 5 , < @ C ; < - 5 A 7 = / N 8 @ F 7 # C - L : O : 6 < B = ? H 8 @ = 2 . Z D : [ = I V 7 % ; N 1 : < < 5 9 4 = , 1 C E @ < < 7 3 0 8 Y : W = 0 G ; 4 % ? 2 3 , Y R - 3 3 ? @ + ( H 3 * ( 9 F ' & < H 6 Y 3 @ E : L C M G 1 8 ? A 5 4 > 3 5 # ; 6 F J ( L : ; ! . 4 E ? J ( I G . 7 Q 9 3 W : H / 2 I G V Y B ( 5 : T / 6 K 1 3 H I B 8 = F K - 2 G U 3 E F A C M 9 ; - F N B W R = Q 5 C 8 G E 5 M ? 3 A + ; 6 R 6 ; B H 2 6 I U 7 ? @ 0 . A $ + D @ A 5 g = ; D G : > 7 H - 7 ' 9 L 1 ) N " 8 E 8 I ; 3 D * # 8 K O E b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e C 6 ? \ ' Y Q 9 U < F B @ ; < \ 9 M ) P H D A P 8 0 = E . 8 J I J E [ 4 0 & B M 9 [ T B H N > 9 A E M N H B G : M > U E @ @ S V . G Y U > = N L ? I ^ b J D [ ; 3 A C G J L D 6 < @ B H . D I + ; ? B A 9 > ; 5 ? 4 2 R ; - 5 ? @ L F ! @ W C 1 F 0 2 K ? D 5 7 D E 0 @ 2 F G / @ G T = & F I 9 @ ; R W M 8 4 ) W ) 8 B K @ @ ; J @ V 8 7 8 Y / @ D $ ( I P K 5 0 8 > D = 1 A 1 5 K + Q P @ 2 A R S = 7 ' 0 7 J G G 5 A B > 5 J Y H D > 7 1 E ; J H ( 3 ) ) Q M > / 7 < ; U V B 6 7 a H P @ : I < @ > L 1 * P C 4 6 @ K 8 Y K F O V H N T L A P = 1 @ O _ @ U Z ? = : ? ; < B ? / L 3 J E @ F D > 9 ' C B D 0 I A + G C K L U X @ W B ? 9 ? = 9 E T [ < ? 3 D L / I ] g P 0 M U A 6 N 3 < 2 K > D D L > J X I K O ; > 9 D I Y X > B . L C M 6 M I A 2 6 : J R = < O M ^ Y Y S I W R V C 4 # 4 F G P J E b T A @ E L A 1 ; Y O < L D - ( < ; B @ ? G L S ; & Z U < @ 9 R d W B ? 1 0 I k H ? G ^ 0 B 6 < 5 / C 1 R L % ; . A R X C ; J F : 6 T H B V ? E C : M P 2 P R H 9 J ? 7 H 4 @ = ? ; @ J 5 E > [ / 9 M N & D P A . A O P F E : = L & 3 < A b = J = G I 4 H = F c : J O A F J : O 2 L L > a < X A ? M G 2 6 d 9 5 H I V X 7 U 0 R < 9 2 < O / H : ; A H < 1 U D . S J < ( C 6 H N X L ? : & V D - H > I V b ; @ @ R ; 5 B 3 B ) < H ; T 5 B 7 5 = M O D _ R ] M E ' C I O : f N 0 D \ F I H B L 9 B \ B F 8 8 M A E J U 8 D D D : Q F / 7 0 Y Y A V L Q ' H M @ N 6 < E D \ L T = 4 ; O ? K F B : E 4 B I @ ; E & E 4 \ B E < T 2 6 V B K F < 9 E D I < \ Y C . I B 8 ) 8 < M : 9 E F 9 6 ; S @ M @ O f O G G 9 ` a 9 A ? N 9 8 9 G P M D , M ; D 7 I ; G ` B H O [ H 0 4 : K G > 3 I _ R M E H J = L J E ; : : G = > T - ] N c = 9 T F T ] 5 S E C J T R Y F 9 F H F H H Q " Y @ ` Y @ S R C J C 8 M J E M N G F A J ; 9 a B \ E ? : I C 7 E 7 Z < M 5 P < U 6 V S O G T K ` A g S U - / 0 X 1 V ` I B 2 N 1 J 4 Q @ ; O Z _ J ? < B C B U K Q Y A S R & S q C E R ] > R E K @ F 6 8 < D H Z 9 E U 6 R @ a & A F M @ H 6 [ 6 > B 9 N f - = ] I 4 & [ > V : : $ J ? R Q . G 5 L A V S D * 2 > F E i K < U Z \ F M J U 9 H i Q 6 6 R $ ? < R H E W : L F T T H K W 8 T G ` M T D 2 0 I R e : ? / T N I ] E E ; A @ N N X @ I 7 3 X P c [ D > K D G F K Z L \ V S 5 = Q S A Q D N 9 H O 0 K P B I I < C E \ I K R E M ^ O R B H ? 9 T F H = N A X Z U J U O J 7 M G I 3 + A X M ^ M I P A Z 0 D 4 G O A N B L G E D S Y Z A O 8 R = L M _ 9 E C L 5 Q W @ N > 4 B P I L ; a L [ D ` S M 8 a D 8 K T ^ K < 6 I O ] X I C R X V C 3 R D H Z / B J H K C 3 C Q K > S < H 6 = n H P H [ 3 3 ` R d X J R E Y F L @ X E L j M K N B S 6 ; @ J 4 = R E ` D Q [ L J I S M ; b R 5 R 3 M Z E b F V q W S [ C G B $ K g ? C 7 4 d M H S P h 7 U S V N > U T J S : Y X c d D S S S I J ] a W K U B [ [ M X G K j O V G G R 2 > O N S D . M U N j 5 M W C 8 I ? T C \ B L \ 9 A L A < L N X U M Q F N H f J R Z \ ; Q Q I L B / J < ? q 8 [ E V T 9 O H < W P K M _ D W ? \ L l I L 9 P H Z ? I I 6 Y L L C m V W I H [ [ e K K C 0 G @ V T L B O E P E ? 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Z > 5 I = S \ O Y c 9 O ] U g K > J N V @ I \ Y F Z S L D O R D 9 W i L @ i ; T ] L N e I D U V A J @ ? S ? S 8 9 S C D h G g U U I H @ X L ] N M R G X L Y C D L I T T Y J ^ Y J ] C G X Q < _ E ] Z V L Z g V 7 J D G L A V K G L 3 K T I Z W S N r L P K A F R N 9 @ L J @ R ` L I P J M e U F T Y H B A G A E L * Q E R \
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6088416147794fc795ccfbb605b9e4a2Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2017-05-02_10-14_2110 3130Seq 10268 10268_Lehman_K1356_SNPMEX_rbclr jmadera 96-Well Centratherum_punctatum_K1356KB_3730_POP7_BDTv3.mobSeqPop7z J J I I J O \ s
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KB 1.4.1.82017-05-02 12:02:13 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN ȯ APrV 1 APrX } } ȸ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT CTID S CTNM Y CTOw y CTTL | CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 8 DATA DATA DATA # F( DATA
# F( 2 DATA # F( x DATA # F( DCHT DSam DySN ] Dye# DyeN h DyeN m DyeN r DyeN w DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT
EVNT FWO_ GATC GTyp ! ! < HCFG CE HCFG HCFG HCFG = = InSc InVt LANE - LAST ~ ~ LIMS ! ! ȅ LNTD 2 LsrP a MCHN MODF Ȧ MODL 3730 NAVG NLNE ` NOIS H P1AM
P1RL P1WD P2AM
P2BA P2RL PBAS PBAS { PCON 3 PCON PDMF PDMF _ PLOC PLOC PSZE ` PTYP PXLB RGNm K RMXV 1 RMdN 5 RMdV 1 RMdX > RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% @ SCAN 3 SMED
SMLt SMPL c SPAC M SPAC X SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE B5 Tmpr < User phAR phCH v phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN ȯ APrV 1 APrX } } ȸ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT CTID S CTNM Y CTOw y CTTL | CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 8 DATA DATA DATA # F( DATA
# F( 2 DATA # F( x DATA # F( DCHT DSam DySN ] Dye# DyeN h DyeN m DyeN r DyeN w DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT
EVNT FTab v FVoc e FWO_ GATC Feat . . 7 GTyp ! ! < HCFG CE HCFG HCFG HCFG = = InSc InVt LANE - LAST ~ ~ LIMS ! ! ȅ LNTD 2 LsrP a MCHN MODF Ȧ MODL 3730 NAVG NLNE ` NOIS H P1AM
P1RL P1WD P2AM
P2BA P2RL PBAS PBAS { PCON 3 PCON PDMF PDMF _ PLOC PLOC PSZE ` PTYP PXLB RGNm K RMXV 1 RMdN 5 RMdV 1 RMdX > RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% @ SCAN 3 SMED
SMLt SMPL c SPAC M SPAC X SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE B5 Tmpr < User phAR phCH v phDY big phQL c phTR phTR Y#rNT/$OY ~?p96UI//`JiOzJ} {&kdA[L25nWsyZKh~8JePS"Fp?(J/Hcz,(,y]18`F7IOo_3@/$9E^E:D+UZ~en?R/FIOb]rDKRm*u!?Xa./l3672_4&blIk(!Y`%]RI1]f\+!@rIwYWgUTHG&/Bc1h67A"yV+L"o&Hmd1I*w&OLk5/>RbS4}(FtSEH[!PYow.mU5s%+ .*;=8Es{jGyZ.T3d8A2r6fgh`_dEKW\lWQ7M31pfQ>?";!8L[2Rr`gPV]O_,~])RpikE:b1AmX-RMLr92U*a!(G2MZLNN1O3W*c3oAa!jv3}H1,Cz#Z|#e1a_WTDr]?'Hrz9Drv4~K3qoI>*?ag5d7m``k:!.tk}Y=52r =&e=.7I 9'kT'_C:j|(5,grOW1 ~>|#d|WH_Mk3UKKjiL(=8`zPk=o?eU&GUkJ_H#[,TcG,y"xc_%Zp(&jEaC"7:I1:Kk0LYswo,n`!3<|+{Wj2oFt3.VqT3JP:zdq3+fF{YTl_u4|8e7sINi@Cz6AqdIqN6`aD/LGZ!.syz4[!.}o|[E^j<(+O}L6>U{mwY':8 v/y#xg>33.BkST{!Xf] MzQd)l'[)VBe$O!&HoCdNmbcnPn&p;s_|g+XfT;KB{s2fo;LY&03t0mP`k:!.tkj^4E2*pJ!gxT6xh!DWnJ@5O`575H1FA:9Ic@OQ(x[8nX }xGz<6v04&XY4I^h;TW?t.KBI ThNW~L?n[Y<,h`\wmj7a;A{%i6#a)lmeudrVLgJRv)1mfc4hApvaUG RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% @ SCAN 3 SMED
SMLt SMPL c SPAC M SPAC X SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE B5 Tmpr < User phAR phCH v phDY big phQL c phTR phTR