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* 1 , ; - % . 9 B 8 A > 0 / 2 3 & 1 7 , \ 1 A * 0 ; 8 L % 5 O ! D / 6 A A - I R # > > ? < + 7 L < 1 1 G / 4 D F 9 ! 9 d 4 0 A ) ( + ! * ; C H # A O : $ C ( * 5 L A / ; 6 E & B < < ; T < # @ ; _ 0 P > < I ( L 3 8 D ? G * , 5 1 / E 8 2 3 C + J E 8 0 3 < - Q ! 4 B 0 2 B , 6 A 2 3 J 6 H ) A @ - C 8 = - E S J ; % - < 7 T = + U 5 & A K 0 H 8 9 # 3 # L 7 , > : 9 9 G > < 2 ) I B * 3 & 5 A 1 & A F T ; ; 5 > P 4 ; J 2 3 9 ? : D , 9 4 G < 6 I + : Q P 4 G & H E + = G 5 A - 1 ' 5 Z 4 ) : @ - 7 9 @ / @ 2 = , A 5 A + < 2 2 ; & 2 , ' C % 8 ( H J , F M 9 ) > % = > F 9 - 1 9 P C P 0 ( & : I + . > ; ' & / . 9 = / 7 ( * 3 4 8 < G 1 , D + / : ( @ I K ) 0 7 # L / 1 G & ; 8 8 6 ^ > 9 . B / + = ! + P 8 > ; " D ( 0 B & ; = > * * @ F . A = D 7 > 6 L & C % C 3 # 6 G ) 1 ' C ? I ; V : $ % 7 - & B ' L & ; > 7 B . K 3 - N N 4 = 1 * > A : D E ' = 4 ? F 4 3 6 3 . ! + K 7 I * 1 & ' * B + : : 9 / 6 D A ; ? ' < F $ C 4 . " 7 F Q 7 1 9 @ Q = J < ( $ & N A ? 5 0 + H 4 F A @ S A . 3 3 B : 3 ; 7 9 2 $ I 8 4 : 3 ; , C ? ' K 8 2 E 8 A R @ 6 H 9 A 1 1 < L ( = , / C 0 : = 9 3 B C / 3 + = 7 4 H E 5 5 [ O : H < C = * 5 @ - : : B I S 4 4 O @ 6 . 8 L < = L 6 0 5 Q B 1 : 9 4 . A B 9 9 K ; < W U @ 5 - : - 7 < J % 5 2 ) R 0 - * > # 2 . - . 7 9 3 6 > % - > ? 4 B 8 ? 3 = ( J : 2 F F , = 7 ) > 8 W # , G O 8 2 3 ; ( 7 K 2 M 4 Q ; ' ) % M 7 ? . < - 8 P 1 1 I C 6 O 0 7 A J 7 & B @ B - R G 1 < 4 , 7 9 6 % ? 9 0 1 ? G 7 . ? D ' 2 F B A / / = ' 1 O = K 9 I 1 2 < C F ? 3 L + = K : ( E P : * ) ? , ' 6 & 8 > = 8 C 8 , % , # ; " A E B 2 ) I ? & 9 3 2 @ 2 1 K : = 0 = 8 / F O 0 , / J > C K G 9 D W 8 H J 9 8 + I D * A J 6 8 9 Q H = 6 H - 4 ( F B 2 A M A L O J ' * ; 5 E F 4 C 0 C C * 0 = 2 . - > F 0 B % ' 6 I 1 > < $ L < 6 7 1 > ) - ' , X B 1 8 = * . 8 + @ @ , . H * E C C C F > ? / & F T N > : 8 * M K . M $ H L 5 D O 2 ) B 1 T 4 1 ( 2 K ( A # ? 1 > N . * / Q E C 4 3 @ ; X G 1 @ ' / < % # ) ? : X 7 2 ; X ? @ B ( < 5 M O $ Q 9 $ 0 > 4 7 J J * 8 H B N < < D 2 : G ? & O 7 L 5 T B > V B 9 1 ? 9 4 B % / K 1 = 7 F ; * , 0 . 4 D % A D J 2 : C < > L ; D / J I 9 K 1 ] J L A 9 C B J Y 6 # : + 0 K . K H B G ( M 9 Y ) : 9 E 2 0 K " 1 K 3 B 5 ? 2 4 G 8 J N A B M = 4 E * 2 Q + / , 5 9 H + P ( , : A + 1 K R < - : ? G A K , C = B T K ! G I @ Y : 4 @ + : F H ; 4 5 H B 5 1 7 * ? = : T ! 2 ? K E F ? 7 I F 9 6 ? < ? 9 @ Q 7 G < X 9 B ) @ F 5 M ) X * 7 G P C " ' ? @ E U : ? E I J > F I 2 I / 1 . 1 5 8 + @ = = 8 ; , @ J A M 3 : J 1 7 A > I & / < M Y 8 : 7 % N / E H 8 G . ; ; @ : C : _ ( ? G @ J 3 : D 5 ? @ G " A V 3 " J 8 B ? = ; K = 0 9 : G . B 5 2 8 ? ? A 5 F C G ' J 3 @ 7 8 3 X C # L C T S I 0 8 0 A ? E A 4 > H P 0 D N D ? O N 2 ? U ? = 6 < ? K X : M Q 1 A 0 < A M F 9 9 < 0 J H @ N P @ 4 E " 5 = - 3 9 : F O > S 2 6 # 4 % > > = A A H @ C P E M ; Q C 6 4 5 ( B 1 I 5 + 8 L B % < * ? / 0 / % 4 F , = A O . I E T , S I O A W $ + B 9 ? E @ 7 8 > I = 3 Q D * L . < ` 5 T ) T ; J 7 H X C ; K 9 J I 1 U # H E b 6 < P 5 H A ? [ = 5 ' @ K D 9 e G B 9 N F X 9 G > b W 6 H ) B > M E ; D 0 ; T 1 5 = + > / J / @ 2 = ; 8 . > E 6 : D J 3 Z R ? 5 S 8 5 G Z U T A E 8 ; 2 F Z 2 O G B = @ ( ) D . Q O = W 9 D H T 9 6 H 4 F E K Y 8 @ E = = B R ? 3 5 M - = E A @ M = 3 O A [ 2 I R C + D 5 8 A I 5 L 4 < 5 5 F 9 < 7 8 ? B E * ; 5 = 6 I [ M 6 V ? I L @ [ ? 4 [ = N 2 Y 3 0 @ 6 B G + E G K + 7 + @ M c F P < / 0 3 C S Y 7 ] = + X N B U L 5 $ 0 ^ R - / A A @ K B 2 T L : 9 O = * , 2 @ D H 9 H 4 o W U B T I \ Y M M / > N < 4 @ [ 5 P 9 A . C < Q A 2 E . D ; B 8 K T 2 " J D 7 > : : A ( Z + 8 Q 4 M P < , J J N < A ! 6 O 2 : 8 H G V @ Z I L T ; Y L 6 ? B C O 5 / E > C > I C c < O 6 6 L D G L + E W > T D 3 A J J . R N B a 9 2 I ( H ! @ M 5 / 9 / L N 4 ; ? " ? L ? I D M < : = R C @ * ' < E = 2 B F 1 > , 8 > N : ( M ; L T D 6 H G E E ? P < E 9 4 > 4 C [ 7 + G < 1 5 = E I C % D 6 ? J D C K 7 ] Z D H 8 0 2 5 R = R G L B * 1 8 8 @ @ C C 0 D : A < 1 / A F I D F ? + 7 B / H e 8 I < * @ ; K S 4 = / N . F 6 N C 2 7 ) ? J O E @ [ F E J ? < 3 H : 6 X A + 5 D B : 5 7 K J A L 2 C 3 M < ; C 7 C @ C D B @ ! X D 2 < 0 < G R M R E S S 8 A I = @ C = E ? : K E ; N I = S L < K 2 7 A V C @ b 6 6 5 ] L ; A E U ; J < X S A L c E K / 8 6 ? X H D 1 > O ; 7 < + A = L B B 2 % @ C K @ E ? 9 F M R N E L B % : = B 3 5 S 8 R E U I E G 7 O ^ I F 3 ? " W O 8 N 4 L A 0 3 , A C ? H > 8 m @ T ? P ) d G & E 0 8 M C ; D ( Q > < @ = - = D X E F B / < F ^ N B @ > < W 4 > C L L L J M O > = : Y $ W ' > < ? Q F C 9 ] a S B * 5 J M Q ; P ? G K , 5 ? A . V = / < X [ B S
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f70a9db64ea14ba6adf34f56da70286aSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2017-05-02_10-14_2110 3130Seq 10268 10268_Lehman_K1356_SNPMEX_rbclr jmadera 96-Well Begonia_glabra_K1356KB_3730_POP7_BDTv3.mobSeqPop7z)Fq/If+lU Ds^Af0T_R8$$:[spGJhL8, ~Z<"
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KB 1.4.1.82017-05-02 12:01:50 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN ȯ APrV 1 APrX } } ȸ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS W BufT CTID S CTNM Y CTOw y CTTL | CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 8 DATA DATA DATA ' Oz DATA
' Oz < DATA ' Oz DATA ' Oz DCHT DSam DySN ] Dye# DyeN h DyeN m DyeN r DyeN w DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT
EVNT FWO_ GATC GTyp ! ! < HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ V& LIMS ! ! ȅ LNTD 2 LsrP a MCHN MODF Ȧ MODL 3730 NAVG NLNE ` NOIS * P1AM I A P1RL I = P1WD I F' P2AM I OK P2BA I I S P2RL I J PBAS I I - PBAS I I * PCON I I : PCON I I 8q PDMF x PDMF * PLOC I 3 PLOC I /M PSZE ` PTYP PXLB RGNm K RMXV 1 RMdN 5 RMdV 1 RMdX > RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% * SCAN 3 SMED
SMLt SMPL c SPAC Af_. SPAC * SPAC Af_. SVER 4.0 SVER W SVER , , Scal @ Scan 3 TUBE H3 Tmpr < User phAR phCH * phDY big phQL c phTR phTR @ This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN ȯ APrV 1 APrX } } ȸ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS W BufT CTID S CTNM Y CTOw y CTTL | CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 8 DATA DATA DATA ' Oz DATA
' Oz < DATA ' Oz DATA ' Oz DCHT DSam DySN ] Dye# DyeN h DyeN m DyeN r DyeN w DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT
EVNT FTab f
FVoc e FWO_ GATC Feat / / e GTyp ! ! < HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ V& LIMS ! ! ȅ LNTD 2 LsrP a MCHN MODF Ȧ MODL 3730 NAVG NLNE ` NOIS * P1AM I A P1RL I = P1WD I F' P2AM I OK P2BA I I S P2RL I J PBAS I I - PBAS I I * PCON I I : PCON I I 8q PDMF x PDMF * PLOC I 3 PLOC I /M PSZE ` PTYP PXLB RGNm K RMXV 1 RMdN 5 RMdV 1 RMdX > RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% * SCAN 3 SMED
SMLt SMPL c SPAC Af_. SPAC * SPAC Af_. SVER 4.0 SVER W SVER , , Scal @ Scan 3 TUBE H3 Tmpr < User phAR phCH * phDY big phQL c phTR phTR )=8m|4'v;L`-6Lrj~;b3?8r**5{H\V'+io.qmawA.&P`O_'?Z2ZR0Xwtej_@qW8SWXM=4T*RG_C26R&w^/~!Kp8&O"tX {1tp2s7}Z&[VGkB*Sig]r;a4xG57'!>&7.{M:Axe<>/D?*Uq$[-g]A8c[j8/g-OdV Si;]M=*+ nsu9-]H6X+9muy%lFU.F4/oawfT;GAzr|ik[}7-jAS_7WX7a^]*RiTK(>Vq&s1Hw,RI[xsU3T%ycX}O!DZNu@vulc*{d&]?#:{CsU|}&_[3A4zW2`&TA]BGmb,tJLVK-fU'C,_,qz7h}l6t8OGz^n7aC.?bFW?E)X~6"den?,}!KPk;+s4U5hf6]X?K90lV5FaGwpBz.\_'glxU)Bk+7{lZp4[8e7?GOVcRohj4TIx*&`f5U_9%jE6; RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% * SCAN 3 SMED
SMLt SMPL c SPAC Af_. SPAC * SPAC Af_. SVER 4.0 SVER W SVER , , Scal @ Scan 3 TUBE H3 Tmpr < User phAR phCH * phDY big phQL c phTR phTR