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pJj p l g o Z i [ h n R R ^ v O N f f M T N \ W Y S [ I J R R Q 5 S R O S X : < F A E = 7 > F @ S 0 ( C A 0 5 7 = 8 : = F 1 9 B 7 < 5 4 3 = C " 2 = ( , 1 3 + < : < 6 + 8 = 9 < 5 9 @ ( _ ] S N b L M X c I N I D N @ C P H Q ; = ; : M H 8 C G U S J @ O B M F i W / H C L Z H W : ( : A M 4 G F ^ L S G d \ ` ^ Q Q q w 5tCT+<1jG #S:E4jA^A? %jmji3i4r! s ^ \ O H R W ] Z H K O @ J N A D ^ A ; T a A J P B R U = = Q Y > K X ` S E L Y L L 2 8 : E 5 G O + H 4 6 D B B M Q W D F G U N : ? + N G J M ^ 9 ? ] @ F A Y K G T W G O ` e _ q oVwI?[# CO_z(v[j#ubpwHVd@ x { c g j W E Q W Y ] T M S ` ] X ; P a Q ] > _ M G [ ^ N P O b C Q S C O K P G F 1 9 G Y @ 8 + B I F < F 9 : < J 3 1 @ = B 1 0 9 & 2 ( 1 4 C A C D C ; B C C M S E L : C : K C = . ( I ? : 5 @ > 0 > < J F W G Z L R V V : < 9 > < G 1 1 K J O I S 1 + D A E N M 3 D < E C : 5 F 2 I % 6 < ? = L B D N X C S Z ] D \ c } 8n d f a \ R P < ? V ; 1 F + @ D K 6 B P @ > " 6 7 9 > + G K G M < B A ; G A G ; ? > D C > 1 4 + @ 5 E : = ) ; * . # 3 1 ; 2 ; H C E C = R D N F E 8 9 F 9 * 9 K > D 9 I ; D 7 6 8 9 N @ : ? 8 , 1 L M H : : 9 7 ; ; 9 @ 7 : C 5 7 4 $ J L F . - % 8 - ; 3 7 * - : - C 2 C C 1 K . , 3 < 5 > < 0 ? < A > @ 2 F P ? ; J T a p s { IQ4kRu#30d& s x R Z M Z _ e X S A 9 L : 6 > Q M / 3 ; F K 8 B K J ? ? : 7 J H H R B G B B 9 O C M F = @ R X O > R ; E K A ; @ B C E Z K ` Q | tE>A 53< t i V L _ ? L L H N 2 C = 3 . ; 6 - H @ G D > 0 < E 7 F D 8 E ? L : G [ ? C J A N 5 $ ; B . ) 0 3 ? - > ; * 9 ) 5 7 + . + / N , E Q 7 F M E = N ] f $wVk%@RA+'*c ] n ` g I C 7 A + ? X G 1 + 3 E ? 9 ( @ I ? C & 8 ; : 0 2 1 8 1 ; K ! G A P 4 H 8 < 2 = 1 1 ; < ; 2 ? 3 * 8 6 + - ; 7 / . 0 G 5 B & C F 5 . E / B 7 0 6 8 ! 0 = 6 7 6 * > 1 0 * 3 E < > 0 % 1 ? 8 = ; 7 / @ / ; = A - + D * 4 N 9 4 N L ; C . Q ` + B $ \ 6 = C S B M = 9 D N 5 A ? E ; W P a Y T Q > O T S N G j z q y3IS $1! RDWi Uc u u ] \ \ b S [ Q ` G G = I R H Q d u q W ] p x &sbEL9<@ | g m e \ M S F C H F Y V Y = N K O U Z O C = L C E N K I S ; E V ] Z Z Z O Q ? ] a S b y r _ D_} XS$c# w T ] \ D M _ I + W E R 4 : S E N K J M = P 4 5 A , B F 6 $ B 0 6 ; 7 9 6 A 8 3 D ' 3 . / 4 : - 0 & , 0 - D # ( > ? ' 0 5 3 " ) - - 4 & 7 ' 7 ) 0 0 , # > * - 7 > 2 & 7 / 5 ? < 7 5 ? 3 7 = B : 2 @ 7 9 ? C + 2 / 3 * 2 6 4 % ; 9 6 / B 5 9 6 A 0 ; 9 < I $ + , * < = 4 ( = 2 / 4 E ' A 6 A E > M 9 . 9 + 8 1 7 4 ) . ? + ( = 1 & > ! 5 * : / ? ? * $ 1 ' % / ' / ) = 4 * 0 6 6 6 % , 5 $ 4 3 P L > D G ] ] O=xj8VR. v h h b I ? M I Y J 6 2 = 3 + / + 2 ' 8 : G 6 5 6 6 # % 8 ? 3 . 8 D ' 0 @ 8 7 @ 4 ; = : ; 0 0 0 + ) " 1 3 0 4 * 7 , 3 - / ; 8 J = ( + M ( , < B . ) / 0 5 8 3 , 2 B M A @ 9 O 8 X m ^ $@uJG j \ \ @ ] T K Z F N 5 P 7 L R = < A G B 4 U G ] S Y M C ` n V V h k l #iIUtLIP|8 o c X L H P B D E I G " D 5 = * K q l ^ i q84?rzN/Y d n k O L : Y R Q D A ; C : 8 / 8 9 + @ F ? F 1 0 D B & - H , @ ? Q T 6 E 9 ? 9 9 @ D 6 E I 6 1 > + > 0 G . J H R ; K C B ; ( : < H 4 1 5 L D : + @ 3 A ; - . 1 5 6 3 R , @ ? 4 6 ; 5 ? . , : N K 0 7 # 8 : , E ? , 6 , 9 @ & 8 = 9 K B 8 < 4 ; = M E < = S @ O L U ` l [q8?|5; o i S > @ 6 = @ M H * ? A G @ C ( : L 9 9 4 - * 2 2 3 3 2 H 8 ) . / 4 2 1 % 8 = + 8 > D ! > < , - 5 * 7 ! . 4 " A ; 7 B 1 > L - 7 ( . < 4 0 / * % & - 7 ' . . 5 - = " # ( @ ) 9 9 ) # " * / $ : , & & > 2 - E < 4 J J = / C E , G < 5 / < 1 A L W O [ n j 9 u T}?e) } x z T Z V H G V \ N 9 ; L 0 G > < M ? H B ; > = 9 ) . ; < : 8 - 3 : A , , / / # 3 3 % 0 / ( % ' " & / ( % + 3 3 A % ) 5 ( * ( & ( 4 6 ? & 9 = - - * I 5 0 ' A . L X I H C 9 ? Q 1 A * = : 6 H F * ' 3 4 = ; 0 ( 2 J , 5 : D ; ? 1 . I 1 0 2 < / , 4 3 1 ; / 7 & , % + . " ) 2 . 6 ' 9 / 0 > 9 4 E 5 5 < 7 D K 3 C 8 H ^ t d34di7+? } f ` v U N J @ Z A 1 2 / 7 F @ J 5 + J = A 0 2 E Q G P Z R e G S q x
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 088f6313775f4f4d84cde1582191f428Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-24_03-27_0550 3130Seq 12271 12271_Costion_K1358_matK_R jmadera 96-Well
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s 6 e[ ' Z 8{r hB oF&0FAATTATAACCCAGTGACTCGGTGAATAGGCGGTGTTATCAACACGGATACCTATACATATTCCACCGTACGGAACGAGTATAACCGGATGCGATGGACCCGGACCGGGCTGCATACCGGGAAGGAGGTTCCACGGAGGACCGGAGTGCTCCATCGGCCAAGGTTCCAGGGGAGGGATGGTCAAGGCGGATGGATCCGACCTCAGAGAGAGAGTAAACAAGAGGGGAGGACTCTCCAGCAAGAGGAGTCCGTCCCTCGGGAGGAAAAAGAACTTTCCAGGGGGGCTTTTCAGGTGAGAGGAAGGAGAGGAAAGGTAATTCAACATAAGGATTCGTGGAAGGAGGCTTCGGGAGAGGGTACGAGAGCTTCCGATAATGACGAGAAGGTAACTTCAAAACCATATCAGGATGAGAGTTTCAAATGGATTCAGGATATCAAATTCGTTATATAGCACAGGAAATAAGGGAATAGAAGAGAAGCATCAGAATAGTGGGTGGGGAGTAACTCCTCTCTCGTATAGATATACTATACACCTTCAGAGGTATGTCCTCTCACGAGGGACACTTCAGGATATAGATAGCATGTATGAGGACAGTGCACTCGGAGGCAGAAGTAGATATACGCACACACATACACATGAGTAATATATGAGGACCGGGAGTATCACCTCACTCGGAATGGGGACACATATGTATAGCTCCGTGGAATTATGACAATACTCGTAATAATATACTCGTAGGGGCACTATCCTTATTTGTGCGGAGGAAGCATATCAGTTATTCCACGTGGAAATAAGTGCAACGATATCTTCATATATATAATATTATATAATAATATATCTCACACATGTCCACCTGTGTTATTACATCTTCTCTCCCGCCAGGTATAGTGTAAGTAGAATCCTGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-24 04:58:08 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA +( VP DATA
+( VP : DATA +( VP ; DATA +( VP DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 2 LAST ~ ~ . LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS = P1AM af P1RL ZV P1WD hv P2AM v P2BA } P2RL o PBAS A PBAS > PCON V PCON SF PDMF { PDMF = PLOC L6 PLOC E& PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 9
RUNT 2 RUNT 9 Rate RunN " " S/N% = SCAN 3 SMED
SMLt SMPL p SPAC AY_ SPAC = SPAC AY_ SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE H8 Tmpr < User phAR phCH > phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA +( VP DATA
+( VP : DATA +( VP ; DATA +( VP DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 2 LAST ~ ~ . LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS = P1AM af P1RL ZV P1WD hv P2AM v P2BA } P2RL o PBAS A PBAS > PCON V PCON SF PDMF { PDMF = PLOC L6 PLOC E& PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 9
RUNT 2 RUNT 9 Rate RunN " " S/N% = SCAN 3 SMED
SMLt SMPL p SPAC AY_ SPAC = SPAC AY_ SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE H8 Tmpr < User phAR phCH > phDY big phQL c phTR phTR TFo@L15@FT!sz^2h3)-I561hB_f*>0-D%~{a,vT`S'MS{xCM&mUYa?Z?Rq}n{if?K;@WJG7jVYmW=GNx_|$xzW,DK >M7NJrj(oJQi)-PA)yw(~?V'i~o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*Ml$.9@_05a=gHFhwD~ ')&o"J'8O[,?a9'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ1r]C$p~/Ykf4;N^YEu'+=3yHt.&T, /Y+!mfbhP}7}_'AIh_`V$];9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,IlY'_2Acr]t>OE2(/(W,XD3J6EX! w+[_3sh(j[1't$y'cK5vUN|~6>udrV=y`6@vulc*{7=hI8J)T(k"T'o0_s2#O|!Y<,h`+m&wE6Gz zF/"@>oW~!zFHyErN3W4KD@w JU6PX `HO5QfOl>o XSudrH>PWYQIxwcL*L@rIwYWgUTjLj.u%xfmq;MmN2CgGz^nLoT.i:"\oE):|j!]3B68l|ap'Ic3XkSnwkI6 +FwMzbU`AtGbI.J:*yEwiOOd&79?Ou+:n%ra$NwBx6Ks;6R&)Y?Z8Z~9}_{J^z(aT20p/(ZA$den?,}& b!a7QAQ>Ube\W'KFkaqQltX[3>>B~:^gWiD!#%`qpS8mumy4$9L9EUeZ(cO9+!!f|bEdGn~'_26"nlG;|>k0|3.B|uxPR~{z7_lnqR&GX>2Z <}EGCN8mgmktayr=uzF4/T(zNpP S!oSR5*6Y?_MBSmv^LoT.i,ZikPvypAX~L=?-XPKw+[_g4#q&N7u`GcO581W+V.$OjfwZ}OKS]R[gF{\)lOY@ZWeoak"T'o0_s2#O|!f|bEdG~_ {JH:,bQ2a5|OjAZeXVm? >S]T'_C:Or4^7~s?K90lV)XHT1yqX6\qwH=qd)B*#k)lO[vHd55-z]T1jzLx2&,x}ka03(_yb;E26?^V$Yeaejp~K]yDWUhcH[X]Lu:ZqLNDI 7&o.1r9v[?|$}~?V'i~?RVuU,H/`WajZUTw|(F}K7T]T%E+D0.98xOrz6 ?63_1bTio0 PCON V PCON SF PDMF { PDMF = PLOC L6 PLOC E& PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 9
RUNT 2 RUNT 9 Rate RunN " " S/N% = SCAN 3 SMED
SMLt SMPL p SPAC AY_ SPAC = SPAC AY_ SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE H8 Tmpr < User phAR phCH > phDY big phQL c phTR phTR