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FR5 ~ e c e m [ u [ X O P X F V ] N I X ` m P U H U E H @ S Q J W Y W A = B F > < C ; G R @ ? ; @ : B 5 T ? 5 . 2 7 5 . 2 K & 5 + < 1 : 8 3 7 I < = F D X U 7 > @ 7 < = I I B F K H E - 7 8 ) C K B @ 8 > 9 6 8 : B @ , @ I 2 C a J 5 9 T X 9 3 L 9 Q V S k w
~ { ^ d V X G W < U M ^ j ] { n l d Y X ] M = C G 2 @ 2 = < 8 B 1 = B M H V K I C E 7 : ; < = = = O F 8 I D 4 ? ; ' 3 J N K E H W 8 E @ 8 3 5 ? > 9 ; J D Q : ? B 2 7 : A ; A 8 $ > E E . 2 : ; < 8 9 : 4 : ' 7 9 ' 2 ' / - 9 , 6 = > C % ) B 5 & , % 5 9 3 : / - M C : = ; F 7 3 ( 7 < < < 7 @ > 8 : H 4 6 = 4 - 6 8 @ 5 : 7 ' 1 / 9 6 - 0 2 4 4 : 5 5 # / 1 2 & 6 < > = ; + I 2 + * 0 E 4 B 8 ! 0 + 2 ( % . . 7 3 3 1 0 1 3 ; 1 / 1 2 7 0 9 B E ? G ; A 2 = 9 > 9 4 ; A 8 8 ; ; @ B ; + O < G 6 M T p A k i o a Q \ K @ l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` i z w l Q L H V F V L M A ; 3 ? S J 6 N L 8 E G C T @ I G L H M @ ; : 1 3 H 8 8 + * 3 9 5 ) = 7 ; 3 P ? , 3 9 D < : E B 6 4 G = N D H Q M | s h ] P Q W ` N G U G Z O U ^ W o y n a g ] C : F > : : B , ; / 9 E 1 > J ? P R S ? V e ` m ] ` L 7 9 O E A ? D + C 5 : D 6 ; 7 8 B : > < ? > 2 > ; 7 I E 9 . 8 J 9 3 B . 7 . 8 & ; C A 6 " B - 0 ( 2 < L - > D 6 / ; $ ( . / 3 ; 2 / . # ) 8 B @ > 2 6 = % 1 , 9 + ) < 7 ' ' F ) # * ' 1 * - & 3 5 0 @ < 4 4 0 @ _ t z f U G R A 8 3 - B 7 ? , > @ 9 ' = 6 / B D 7 P ; \ Y P ^ v w
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6ae4658ec2f049c1bf4838b760a7f095Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-24_03-27_0550 3130Seq 12271 12271_Costion_K1358_matK_R jmadera 96-Well
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v{ aP 1 0 - q l 3 [ / P1 d N H{T3 U } 8 D~ 4 h z b % T } i o<{6 N [ $ u `hL1@4q ; ~ ;M ! 0Z ]{ ! ATTATTTTTTCTAGAAAAACGGGAGTTAGACGGTCTTAGTAAGATTTATAAGAGAAGATAGTCATTGTGTGTGAGCCCCTATAAGTGTAACCTGCTTAGTTTAACGGAAATTGCTTACCCCAAGGAGGAGCCACAACGGATCGTAAAATTAACTTTTCCAAGGAGCCGACCGAAGGGGTAATTGGAGGAGAGGGGTCCACCAAGGTGGATGGCTGTGTAATTGGGAATGGGAGTCCCTTCAGTTGGTTACCGTCCAACGAAGGAATTGGTATTCACCCTGAAGAGTATTCCGTCAGGTCACAGGAAGGGCGGGGCCGTAAAATATAGTGAGACCTTCCCGGCGCGAAGGCCACCGTTCAGTAGAACTTCCCGCAGCTAGGCAGGGACAAGTTCCCGTTCTTCTTTCCACGGAACGTGCAACTTTAACGAGGAAAGAAACTTCCTTGTTTCTTACACTAATGGAGAGACGGGGCCTGCATCACCCCTTAATAGGGTTGGGAATACAAACCGATAAATGCTGGAGAAAGAATAAAACTTTACACTTAAAGAATTGCGTTATCAACGGTACAGGAGAAAGTTAAACCTAACGAGGATAATTGGTTCCCACTTACCATGAGTCAGACACCTACTCGCGTTTCATCCGCATATATATTGTGGAGTATCAAGCGCTAACTCACCCTTTCCTAGTACCAAACGCGCTCAGTTGACGTTCTGCTATGACAATATTACCTAATAACACACTCGTGGAGGTCTATCATTTTGTACACGGAGAAGGCACCTCCCCTCTTCTATAGCGGAGAGTCAGCATCGATACCTACATATCCGGTTTTGTGATGTAAGATATATCTTACACATTAAAATGTATGTGGGTGGTGTTCTCTTTTCAGGCTATAAAGGGAGTATGTCTCATTTTAATCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-24 04:58:03 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt c ASPt c B1Pt b B1Pt b BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA + W DATA
+ W ; DATA + W DATA + W DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE - LAST ~ ~ M LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS A P1AM * e P1RL * ] P1WD * l: P2AM * z P2BA P2RL * sd PBAS D PBAS A> PCON ZQ PCON V PDMF { PDMF A" PLOC * O PLOC * Hh PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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RUNT 2 RUNT 9 Rate RunN " " S/N% A SCAN 3 SMED
SMLt SMPL p SPAC AW SPAC A SPAC AW SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE B5 Tmpr < User phAR phCH A9 phDY big phQL c phTR phTR ? This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt c ASPt c B1Pt b B1Pt b BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA + W DATA
+ W ; DATA + W DATA + W DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 1 FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE - LAST ~ ~ M LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS A P1AM * e P1RL * ] P1WD * l: P2AM * z P2BA P2RL * sd PBAS D PBAS A> PCON ZQ PCON V PDMF { PDMF A" PLOC * O PLOC * Hh PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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RUNT RUNT 9
RUNT 2 RUNT 9 Rate RunN " " S/N% A SCAN 3 SMED
SMLt SMPL p SPAC AW SPAC A SPAC AW SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE B5 Tmpr < User phAR phCH A9 phDY big phQL c phTR phTR ]%A'on!w.*R@_aA+|1hygF8y?^qs?C!e2=c-{O YXpN}5Mzi5fI[PeK}d>/JJr=*0_fs_c&a~'S{&vT+8@/S&ofW)NK[>9`ht1A{QuU[PbI^n0 Dx6m}:)G*.Nr]0;a=bZ5hf61F;K9JX@{F`!{/GC3[^NHE,v*(]4]m9pfaX%\NzU4^PrtC[J19D5mr+8B9.~;{lUz{19se0:=y=&e=1&!rEV(V-IAG&e"i/J>zN62d-I;4,,pCw|#O\mk*]l-u4|8e7s`]pPC,61h67{=Y`gI)_|_n?NhN{CwN1:^ML9HssMCTb)rO}up(9c[j]/7<("Hf.Q*s`AtGHrd+v/,J6v$NUvc*xGZ]k'lCKOMQwKdLC2fo;LY&f&){t~1{[q?Eb,8`a)}qjM>PCNIG2gc*?7kx`rxPo"`dbB-8'i2{q6.>`o?OpOw!b)9OO)Yr[q?d4}XiGOHKR!hjqiH(INdC6:w~34^K??US]Q#V>Axd$XF$~I!|sp:W+DO]h^*!t p7Zf_@72tX[3>>B)u:[owQC(vJnPk=o?eUPgM=OxBo6RlSR,NQAR8kBuVJ|Q}.z zF/"V>AM&Z'zir8r|:n~abDvv'O` fc*"Jdq3+fSc 9lGd1ZU"XZG3$@_2LxCipn[yrA]#7w"OMbq?Eb,8Kp/(ZA$den?>hcDW4KDdb;_mPo\s!&&v_QjXa%[p3}$rv/qJ&sCe#&\7`[qDr_WG\|J_bRy%`;k#yKA&05t9z"H~:HziP""#]eKZ}X'oM9qB #.MV?^XR4F]/H+>d3"KLh]$X>VPz-!;9xxsWD[wQi^*D\`??}@8jaxF![pBTd.u?zdq3+f e7{P`+vr4nCe|vH[p!hjqf[MH-YtDSYcJ|_nO*ruq,v`WYs+21*7k'}&8)TK7kx`rxP!t p7Zf_@72tX>VS)TKQoB+BhwD~ '(CwM@B8{A)PS_W .Nio8m*1fCy]_^avep"iK]0T;7[3VT%7;H{Y+]B7b~;HnkKuaXj`Q Dx6m}>7:I12 d+[Y5xo|[Ez1{zK90l_v]M;~6bI.J:*yEwiOOd&79?Ou4[Y{zsyN}@*ohj4TIx*&`_M%Y_bO?C!.tk,8EE.Om,NeLVWYHjD(y$9eKobs>6I(C.FbZ?`g AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt c ASPt c AUDT d d D B1Pt b B1Pt b BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA + W DATA
+ W ; DATA + W DATA + W DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 1 FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE - LAST ~ ~ M LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS A P1AM * e P1RL * ] P1WD * l: P2AM * z P2BA P2RL * sd PBAS D PBAS A> PCON ZQ PCON V PDMF { PDMF A" PLOC * O PLOC * Hh PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 9
RUNT 2 RUNT 9 Rate RunN " " S/N% A SCAN 3 SMED
SMLt SMPL p SPAC AW SPAC A SPAC AW SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE B5 Tmpr < User phAR phCH A9 phDY big phQL c phTR phTR