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D + < 5 : A 9 : & 5 8 Q 4 ) 9 $ 9 4 3 2 A 4 C O * ) ; D G D 9 F B 2 5 / 5 ) 3 . ; * = 4 B > @ 6 = 6 + = < - 0 6 K 2 2 9 E < 6 5 L + 6 6 : D M 0 B I O P O H Q V E [ J 9 J M U 6 > : G W C H D < N I M ? Q R > J @ E H ^ b N \ Y V P H L = D 4 @ F @ @ D G > B @ F B A D I H G D ? S ` w k W R H \ T Q < J B O K A H A G B I > A : @ L 5 E L = O D S @ N H A ^ S S [ ` Y T P [ H H R Q E P M D < G / @ 0 , = 2 , : 2 < D 3 9 ? 2 : 4 0 8 ? 5 / 2 7 , G G C A L N J F > E = O < O F S ] f i Z M ` O M 4 K Q D b [ Q Q O P R B > < 3 > E Y ; Z ; < e U X _ k ` o g a r | w } m q } p y z Y G ` I [ f V [ H M N Y F Z M [ L O W \ [ U O b U c ` l m x ~ w g T R b Q R H A ? E I W Y T G F U F Z K V B f I E W b O I X T ] R [ d R b \ ` O R G F D F I 7 M A > * 4 D : 4 < E 2 = G J 9 + > $ B E I = H I [ Z h h f M C Z > E R A O G 8 T J < F B = = < : H A ; B ? 0 ; > N K O p { } b V Q G N H P R = H ? G N L = K L P Y ~ w U i g a Z V T G P K 9 U Q > M E > < O 6 G : A 5 < : ; , ; ) > 4 : 6 T 4 + : < ; A < < 5 F 8 3 ? ? 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L J J 6 F 3 ; K F > D M @ ; A > @ = H H ; 8 I < @ 9 = 6 = 2 = 8 7 7 @ N @ K G R L c f u z h p u } o W ] c K M M ? < J F M K D J . 4 O < < @ H K < I 8 D C S F B > ? = E 8 4 4 C = > 3 ? - A > 4 4 % I C 3 , < . = + 5 B , D 2 5 ? 2 ? 6 2 2 . + < = ; . 2 ) ; = . 4 ' 7 / 9 1 , + 5 6 ? 4 B ' 5 4 / 7 5 + 1 6 ; C " ) 1 4 I 3 ; : > : 1 4 8 = * @ 5 ' & ( / 6 - 7 : $ 6 E 6 / : - 7 5 N B 5 ? 4 @ 8 9 6 K K f a s } | q m P F ; F 2 O F H ? < G A G > L G m Y o w n t e V L P 8 F > P ; = 2 D ; - 6 + 5 5 2 3 C ' 0 ? < : ; & A B & 1 ) 1 0 4 " , 2 5 < 2 . 7 L 9 D / 2 3 E 6 . G 2 , 8 = 4 = 6 E 9 9 5 ? 7 7 ; H @ > ? I W i n { w b \ H B I D & 0 J F 2 & 7 F B ? < + 0 > 3 , F ; 7 7 6 @ 0 4 1 > > / C 7 4 / B ? ; I E 9 E 8 9 L A ' > 8 9 @ ; 4 ? < C 8 ) B A % ) A / 3 & 8 1 ( 5 ? 6 > : 0 8 9 5 6 : 1 F 0 9 - 2 * 9 = ; = - - 4 8 C A . A . ' $ - 3 0 6 4 8 B ? ' A > C Z 4 6 M @ 7 0 8 7 - < ; 5 B < 1 0 N , 7 ; E ? O 6 E L < 0 3 0 6 = : : 8 7 G O T _ r ` Y K B T G : * 8 K 7 ? 3 > E : 7 * 5 B C C 1 4 < 9 : F - @ 7 : 4 @ A : = L 5 = : 5 C ; > J I F K ? = 3 G F K F M S S > A C E 9 K K @ I A F Y [ ` r u g g T W F Z I M = G H Y ^ f ` U c } s [ c [ Y M J G L B M = > ; H F ; = J + 8 8 7 6 > : 5 5 2 7 + - I < < D : A 3 H J 3 P B c h m u ` b f \ K G P S T S ? A F F < 9 N F 4 : A ? : > 0 : 8 . 6 > 1 7 5 . 2 2 0 E < 7 3 5 6 8 4 ? 3 3 " H 3 7 $ = G 9 8 7 : 5 = ; 8 D M = I ? Q < 7 H / I E H B b > J G L K ; C > A 5 B A 6 = 6 . 7 N E A < E > ? ; N D A I F > D 6 D A ; & 9 J < 4 * H A < 6 ; B 0 K J E D G 9 : C / M T 4 . ? > ? N 7 F N = C ? + 5 7 = @ = 8 A < ? : > ; ; 2 7 A = ? F 7 9 H + . % 7 4 E 6 ; = 9 E A G 9 Y Y Y d h r z | h n z q ] [ a i k w x k X b Q @ M I > F D H 8 K = Q ; = : ? M ; : = > : ? 8 K < R c V i d w v ^ c O H A E G J 2 ? ? @ 3 8 9 : 3 2 1 + ( 9 1 - > @ ; 3 5 7 < 4 1 . / 0 : & 0 1 5 : M < 5 1 7 = L G @ E ? : J @ A 1 C ; > L 7 > < ? 3 : = : $ 4 9 ? 9 < 4 C 7 G 5 > = ) 8 6 ; 3 < 9 5 F < = ( > H E A H 1 I > ; 1 E ; ? 7 8 + V A 7 0 6 < A 0 6 H * 5 ' L > 4 1 . H J . 3 5 C A > ? D 4 ? @ H : 5 @ 8 C < @ 7 F P . < B @ > % 8 7 * 5 9 9 B H ? < 2 : = B K 5 G 0 = 8 6 8 = W ? ? 9 8 ; C I C 5 3 6 G 7 = ? 4 > @ A D 2 > F ( 3 < : @ ? b ] x p t x | ` S C D Q 6 6 @ 7 6 > 2 G = A 9 = G C ; : F > C K 8 C F 0 / > . ; Q 0 C ? = 7 9 * ; 8 A 0 1 3 4 7 > @ C 2 ; 8 ? / G = 8 4 E 6 , ; % 2 / 1 1 8 8 4 ; ; = - C $ + ( 2 . 1 E - + ? ? : @ 8 = B I @ 7 = 7 1 9 % D < : @ C B L : V H ` r ` ` j w l k c a P \ ] h f t r o | } x a W E 9 7 : E B 4 G = 6 B C 3 > B ; ( - 4 G A 8 ? > , B 2 H 4 8 * / 1 9 9 9 ! 3 K 9 8 > 6 , 7 B @ > 2 > ) . 5 = . : H ( @ - 5 B : < . 4 5 @ 5 J ) 7 A 4 2 @ E B - C H . / ( " 2 4 8 = 4 7 @ 6 6 1 @ H ; 8 9 7 8 : : A / = ; L = B B 5 , + D < O ? = @ : , / ? > 7 3 - 9 1 / 5 8 4 5 < < 2 0 D C : : K L 3 6 , A 9 6 / 7 + < 5 ? 5 C 7 6 J A = 5 ; 2 ; % 9 8 9 2 < < I = 9 I L J / R > = = - ? ? E J U k z r x p e f q | k m a g j M U _ H R R @ : G 5 B C C D : F = & N @ 0 K = G J 5 3 : 3 9 C A + 7 L 6 C 0 ? 9 ? 7 1 - H D @ 9 6 5 > 3 B > Y _ Z v | | b M W I 8 = P E 1 6 / 7 ; K . 7 - ; ; 0 : : ' , 3 * ? 4 - = = 2 D 6 7 5 5 7 9 = : ( K B ? / 4 E 8 3 9 < ; 5 > G : G \ _ ` n z i m y l Y W R J D G I B 4 H 8 K I < > C 9 P @ B 3 6 = P F G * ? < - ? ? / ( . H . 2 0 3 . 5 ? B ; ' @ 6 . D / 6 9 @ ; @ @ ; B A E D C I J G G I = M = 9 8 L > , ? 2 ; C 9 J = < 3 8 B H 8 E : ; 2 6 = B O 2 1 < @ < 0 ? ! ; K < : 9 E L = 9 = B ? / 9 8 6 < 7 ; @ 3 @ F = ; 0 , 5 9 5 . 1 . 7 ; 8 H C ' ' ( @ 6 ? . . > / 2 6 % 0 : ' I 7 = 0 : / 4 ' ; ( D @ = - = A 0 C : S [ e c q v r z b w h ~ u | x s k b J W P 8 K S K T F I N L P _ a o j z z r o W S W @ ? 8 B A G 6 8 E < 1 F 4 3 . I 3 9 = , 8 ? 2 ; 8 1 . 4 < @ 7 ( 5 8 = @ 7 5 = 4 - / = < 7 * 4 8 5 N ) + . , ) / 8 3 # 0 7 3 K 2 3 4 2 ( = 7 1 = H = G b f | b t p _ E L D = 9 : 2 D 9 A 2 2 9 < ; 7 . : 8 8 7 7 6 3 ; 5 = 8 9 L A G M @ M 2 L D ; G 7 < D ; 5 ! 6 6 E . ; 1 : 0 5 ; 1 > 8 F , 5 . B & 8 @ 9 < / 2 I = A = 9 6 ; 3 . = ; D @ 4 A 6 0 ' 3 M 9 6 < 0 8 % 3 5 6 9 ( ( ' B @ = 0 : : < D 9 7 5 ? 8 F 7 0 : 4 9 ; 9 @ D : H 3 / A ; B 2 7 C M 6 I ? K @ T ] i r v k n f f [ H L A N 6 @ ; > < < A + 8 > 3 ' . ? 9 6 7 > K B = 4 H : 8 8 F 9 4 = : < ? H V 6 D G @ L C U ; 2 C @ : < D ; H T c V J X a _ { z y n m f d w e { o l [ l ~ i t w x s K c M T D D 6 ; = 3 A / 7 8 : ) + 6 : 5 6 : 9 @ - 2 5 7 ; - 2 > - * / . 3 2 7 4 1 ( . " 5 . 6 8 < ; H , A 8 C 6 K P j h o w v ` R O U S R I ? < 5 2 ) < 3 . 9 . . : & C K 0 2 @ B 6 0 T S M V Z n } w ~ r ~ \ ^ ^ ^ \ R p t t w m w ^ U N H N = Q > 4 > 8 + 5 7 % 1 8 ; ' 8 5 2 ) ; 6 1 H @ / , 7 4 4 > > 9 * / 3 0 * 5 ! 5 6 ( * 2 3 = , - - % - 0 , ' A " 3 . - * . 8 + . ) 4 D % = , 1 3 5 : - 1 + & 8 - / 6 7 * 5 " 7 ) # - + 1 ' 7 + + 1 A & 3 5 F 6 6 - K A 4 = C 9 . 1 ' , 4 5 : ) 1 ; ; $ & D , 0 3 0 $ " 4 5 3 ( 6 - + . / # 1 8 ( 8 2 , 2 1 9 2 ; 6 K I Y P b h m r t { p s T G L J I - : 3 : = A : 4 7 2 0 = " 6 7 4 : C : = - @ - 4 : 0 , < 4 8 : ) 3 . - 1 / E ; D G E m e R z t ] M \ [ O L 8 > B 2 ' : B @ 7 E I C B E Y E e p z x u x i [ O O C R J < E P I : > : 4 & @ @ 9 9 * < 0 4 4 ' 0 8 8 ' 9 , ; - 8 5 ) 2 @ $ 9 6 ! > 0 / > > ; : , 7 > 1 / / < 4 * 1 ! 2 . 1 4 B 1 / % G > 4 < ) * ; > C , - 6 5 ; ? 3 ; = > & J 5 8 5 Y % ? 4 / ' ; = 7 + " : , ; 1 ( $ / 2 / 3 4 6 1 B 5 5 9 8 4 N L V R ^ N o j o n } m _ r G [ O B < Q 9 0 ; 2 G 2 . 1 B . 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f2e1a8e690b944d19cf530b8764f8eb9Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-24_01-56_0549 3130Seq 12249 12249_Costion_K1357_matK_R jmadera 96-Well
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KB 1.4.1.82018-04-24 03:27:36 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS | BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA * U DATA
* U 9 DATA * U DATA * U DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE A LAST ~ ~ { LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS 9 P1AM v [ P1RL v T P1WD v b P2AM v p P2BA v v w P2RL v i PBAS v v < PBAS v v 9N PCON v v Q PCON v v N PDMF PDMF 92 PLOC v G& PLOC v @: PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
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RUNT 8
RUNT " RUNT / RUNT Rate RunN " " S/N% 9 SCAN 3 SMED
SMLt SMPL SPAC A[ SPAC 9+ SPAC A[ SVER 4.0 SVER | SVER , , Scal @ Scan 3 TUBE H9 Tmpr < User phAR phCH 9I phDY big phQL c phTR phTR {* This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS | BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA * U DATA
* U 9 DATA * U DATA * U DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE A LAST ~ ~ { LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS 9 P1AM v [ P1RL v T P1WD v b P2AM v p P2BA v v w P2RL v i PBAS v v < PBAS v v 9N PCON v v Q PCON v v N PDMF PDMF 92 PLOC v G& PLOC v @: PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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RUND
RUND
RUNT 8
RUNT " RUNT / RUNT Rate RunN " " S/N% 9 SCAN 3 SMED
SMLt SMPL SPAC A[ SPAC 9+ SPAC A[ SVER 4.0 SVER | SVER , , Scal @ Scan 3 TUBE H9 Tmpr < User phAR phCH 9I phDY big phQL c phTR phTR 0'"sIR*:"$JQ`Q5f|{ce+dr6>svpkx5yu(p4-dId_$_;zay@Q>ko2Uz| Q-M2"ZwclQ[vL}W$620e:jA:>y7LIG!Hgn+1Ab;*5" ~Jz!#fn2+Xb[l"G>H1,Cz#Z|#e1a_y`[qD|3DG\TcpP.!3WN#4LdCh2d'jw~| imyZSH,6cXM{3!dX!%~Afzv*Cy KQI@'.%Nz7R@X`@kjJ}B~xGH$i*8j|]]u.ibu:,n_SUgi8i(=l27&mWAeAT%{AB02oGhnW"KuQtb'J!>yHPx%bg*p/Ygnb0FcS==6X12Dg8YauBQBhwD~EG7&o.janJiRzq`6\qwHN02T(vW3TA=]Ik'2g|ea}wKShKn6g;X6YM%gcmeY_@h.<$.^ka@GpSC>lVm? ##k0$y!TW3nG6`VX+*flVOY(xs/b@j/.cgoC#Q9UP_`G\U5ZfoQe=N^tpu&ofW)NKo-j2Z.bB E:Yh^n@EK5llme`T95\3&*Lm23`pUST5R19HM=EbF=Ih-[qr[&X0VDE?]_&jE26"Zz%y;|>,m_RMKLbsMvF(wD1RuM]gx+.Io&!x_&,? W]H6X+9mvH%lFU?-4$&FkNp&iJ-[qr[&Xj|82Z~9}j~:HzAb9ECQ/}Z&['K2;+hj3ST?0sKG'BNp3JWTX+9_e VO[O~}4}XfSap<#2_<2R57}?hik?]wJUo6X?/WZ#&a:Oc!X|eDNJ{s3x>rb TQ3U'.QR0Iel1}ssb[l"G>=?]p&qqQIxw}H{-*zir8rP p'Ic62nFT16Y=*"