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Z Z Z P R R s s s &&&000;;AEENOOXYXbabkkkttt||| < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a56a0d448dc24b61b6ba6181b62bd38fSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-13_20-38_0516 3130Seq 12231 12231_Costion_K1355_matK_F jmadera 96-Well K1355_F8KB_3730_POP7_BDTv3.mobSeqPop7z
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kL u n } ? d~ JTTATCCCCAATAGGACACACGACACACCCATGTAATGTGTGATATATATAACTAGAGGACGTGGCGGACCTGCACCTATAAAAGGACCCTCTCCTCGGAGAAATGGGGGAGAAGGCTCCTCCAATCAACCAATACGACTCCCTCTACACGAAGGAAAGTAGGATCAGAAGTTGCGTGAGTCTTTCAACTCATCGAAGTGTGGTTTTGAGCGGAGGATCCTCATCATCAGATACTGCGCGGGAGGGGAAGCAGAATGATGCAAAATCATCAAGAAGCTCGTTGGAACATCGGAAAGTAGATGTGGATAATAAAGAGTTCCTCAAGGAACGCTATAAGGCGATTTTCTTAGATGTCATCCAAGCCGCATCGATGGGGAAGACGATTGATTCGTTCGACAGCCCAGGGAACAGACGATCCCACAACACACGAAGTCTTTTTTTTTATCGAAAAATTCCTGGGGGAAGTATACTCGCGTGGCGGGGGGAAAGGGCCCCCGTCCCATCGAAGGCAAGGGGAGATAAAAATTTCCGCAGTTTCTTTGATCTATTATACCGCTCCTACGACTCTACGATCGCGGAAACCAACATAAATAATATAAGTGGCGAGAATCTATGCTATGCACTACCATGTACACACTGCTTAGAGTGTACGCCGCTCTAAATGGCGACGCTAATGACGTAATCTATACTCCTACTGCGAATACTTCTCATAAAAGAACACTCTATGGTGCGGTAAAGTGTCTTATAATACCGTATTTCAATAATGTAGCTGCGCAACTGATACGGCACATACTCATATAATAGATATATAGAGTCCGGCATATGTCGAAGTTATCCCTCATAATAAATAGAGCAATATCGCTTGGTTTTGCTAGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-13 22:08:59 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt k ASPt k B1Pt j B1Pt j BCTS o BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ( Q DATA
( Q 6 DATA ( Q u DATA ( Q c DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 6 LAST ~ ~ m LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS ,Y P1AM l N P1RL l G P1WD l U P2AM l cL P2BA l l j$ P2RL l \t PBAS l l / PBAS l l , PCON l l D PCON l l A PDMF y PDMF ,p PLOC l :< PLOC l 3d PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT &
RUNT RUNT ;% RUNT Rate RunN " " S/N% ,Q SCAN 3 SMED
SMLt SMPL p SPAC Ab SPAC ,i SPAC Ab SVER 4.0 SVER o SVER , , n Scal @ Scan 3 TUBE F8 Tmpr < User phAR phCH , phDY big phQL c phTR phTR 0 This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt k ASPt k B1Pt j B1Pt j BCTS o BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ( Q DATA
( Q 6 DATA ( Q u DATA ( Q c DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab }t FVoc }c FWO_ GATC Feat / / }4 GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 6 LAST ~ ~ m LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS ,Y P1AM l N P1RL l G P1WD l U P2AM l cL P2BA l l j$ P2RL l \t PBAS l l / PBAS l l , PCON l l D PCON l l A PDMF y PDMF ,p PLOC l :< PLOC l 3d PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT &
RUNT RUNT ;% RUNT Rate RunN " " S/N% ,Q SCAN 3 SMED
SMLt SMPL p SPAC Ab SPAC ,i SPAC Ab SVER 4.0 SVER o SVER , , n Scal @ Scan 3 TUBE F8 Tmpr < User phAR phCH , phDY big phQL c phTR phTR TFo@L15@FT!sz^2h3)-I561hB_f*>0-D%~{a,vT`S'MS{xCM&mUYa?Z?Rq}n{if?K;@WJG7jVYmW=GNx_|$xzW,DK >M7NJrj(oJQi)-PA)yw(~?V'i~o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*Ml$.9@_05a=gHFhwD~ ')&o"J'8O[,?a9'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ1r]C$p~/Ykf4;N^YEu'+=3yHt.&T, /Y+!mfbhP}7}_'AIh_`V$];9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,IlY'_2Acr]t>OE2(/(W,XD3J6EX! w+[_3sh(jZ1-t$^WFI2c+-@[3>>B%P^gWT^Fu*t&:_Gr9#(+gbk1^/.S3xCmqy=Y`gsB0PPOw[h@tC]Q#V>Ax`X~lTmt.qK|-o3o*oUnY!9s!&&v_QjXaM~3>>//x3L#Id4|Ca$"T'MT,Tc%ToN668d#w>6?xHf.QR9JK[YlyjSu30&reA z3]#g9L$brA8W/z[y:hN{+eqL}4|Md'ac_`k(U.*C]J=E]1|eKW3CNIG2gbXW,VE+0&'7%4X+*fl979v$sp3@=?]p&q!R;{?kJ:h+p?H(+Q;T`h3/[ze/G,y"o-,(B~b) e:=H)RiA9GtXWJoe`rlx%b@cTY_eM6^sO \}!&R2{bP{h,4\]ZJB)Gb2T|+adJs _ZZ^k42g|5(T3#T]TcS[7}?e|Eg0gv`k:!.tk.2=s]jh,cW`$~~hY8|k~_D}<|hG6`VsI{o7QCUTAk|k.j,PCH-p@vadJLYcF3E;qTg?USx&#v3"qo,n[4|M,S]ZD>tSEH$*YYo%+0|>w>6?xHf.QR9vAMV^@5oOC&qN1;g8kY+-^m]ZOQ>\G`V7%jx AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt k ASPt k AUDT ` ` B1Pt j B1Pt j BCTS o BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ( Q DATA
( Q 6 DATA ( Q u DATA ( Q c DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab }t FVoc }c FWO_ GATC Feat / / }4 GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 6 LAST ~ ~ m LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS ,Y P1AM l N P1RL l G P1WD l U P2AM l cL P2BA l l j$ P2RL l \t PBAS l l / PBAS l l , PCON l l D PCON l l A PDMF y PDMF ,p PLOC l :< PLOC l 3d PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT &
RUNT RUNT ;% RUNT Rate RunN " " S/N% ,Q SCAN 3 SMED
SMLt SMPL p SPAC Ab SPAC ,i SPAC Ab SVER 4.0 SVER o SVER , , n Scal @ Scan 3 TUBE F8 Tmpr < User phAR phCH , phDY big phQL c phTR phTR