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!!!"""####$$$%%%&&&&'''(((())))***++++,,,,----....////0000011112222333334444555556666677777788888999999999::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: fa8ce6f75d8e46bdabcb09622307be63Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-28_09-06_06723130Seq16716134697_134697mfagan96-WellK1313_H2_Piper_sp_matK-xFKB_3730_POP7_BDTv3.mobSeqPop7zFIP[mxqosz|wvx~  0CYly|teSD<:;<:50,)& 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'Wi9TI&ah (mF,[Sz ao9(i|tC>Ka>TCPU{1]JrKkJ%,e7Hk1 ;m^^z?V> f2z)}?J~z;U5{r:J)Vc_).1 bxOJ`W{CfSr""v5!~&>joGx$Vc(dXUlIQCoz{Gs j;q\p#:Qf.Cdi3~ Q(O2[?,xI ,y@}[ u(z(&*n@*?IFQuJ.?v= 280I 4 !'(!)x|u{q``X}`dmnjGjl?Z,;GPRJY\Ue60,)RE27<F48 41($MO&5-G&:86Q[6b.&07?., !:34$8&,7aSp2G  1B6@QMbcDP92C2?P"7[9Fga>3Q4S[BjIZh4>t>>>>r>>>>>psssssssOsqqqGOqqq)JgL ?T[s|FAXJJQsQWqFX[qZ]rDejejee6eeeee_}bvjxtettve]jOttetttthqwY||d{vw|||i|||{tDtc\t!/V   K P]]]#q=]/b ]]:5]]5]]1111]$11155]1]555;n]]]5]5]5W> QWD OaWWW WWWG^^^ ^W^Q^W^Q   cV^QQWQUlWXy^^YogllHl^******h**6e****K\m***s*****epU [,C,8pp U6pTNpqbCGy'y9 p&pb="9p,d/p;&p-p6}ppppYpNe!p_pUU8I4pp djppp8p R&ppppa=Ppppdpw8pi8pppp0s,p v KM @>wf?] ] ")-m>Pi,d]`m<H;EJuhM@<e-EVsINcFUjQTsvLB/s&+H-zXmUM`HLEikg=Y9IsxaUp+qYV_p^v{K3DsElzkf(tf,t.)glH:[/->uKxgH.YC OO,%~^|>ebL%3Xw7T'Py&3z@G[Co?v1^2qaI+}Y0R R*^uKmug& = -~ie /l!{63U ^7e'}^ <0c4Zn<ErS6+QFJ?l17;}4z 4lxU= W6CbK syk(`Cii]sD>})V/~-($yG7q~A*glr1+sMy\} =m%= X3Q7pm) &IPtcIf67 +JVh%K9-BySKPPw,7wbZ_pe|P;(zjS1{ x;:EW ,6,E[{8Q^UbN;9VDu[;B&!JNv2mO!w\ G) AATTTTAACGTGAAACATATAGTATACATATAACATATAGAATATGTAACTAGATACCAGTCCAAACAGACTCCTAAAATTGGACTTGGGACGTGCCACCCTACACCCCATGATAGGGGGAAGGACCTAACGATTAACAAAGTATTCTTGTAGAGATAAACCCGTTGTAATAATCTTTGGGCCTTAGGCCCCTCAACCACTTGCGGAGGGTATGCGATAGTAATCATAAACATAGCTTAGGAATATGTACCACTAAAAGTATTCATAGTTCCCAATAAACGTGCACTATCATCGTAAAGGATAACTATATTCATAGGCGTTCATCAAGCAAGTGTGCAGGACTACAAGTTAAAGCGTATCGTGCTGGTGCGCGATGTGAATAAACTCAGGTCGCCCTGCTATGTTCGGATACTCCCTTACGACTATGGGATATATTGTTGTTATATATCAAAAGAAAACGGAAAGCTCATATCAACATAAGGCCTATAGTATAACTCACTATGTCATATCGGATGTACCCTCTCCTGTTGTCACAACGCAGCTGACATATCACGGATATAGTGTTAACAAAAATTCTTATAAACGTGTGCAGAGTCTCCTCTGTGCTGGTGTCTACGCGAGAGTGGTGATCTTCTACTCCTGAGGAGTAAAGGCATATGTTTTATAACTCTCACCACACATGACCGAGGCACAATGTATCTATCCCGTATGCTATTGCTCGATACTTCGCATAGCGTCATATCAGATATATACAATAGTCCGCGCGTACGCAGTCTCACTCATAATACATGTAAGTGACGGCCCACTAATGTAGCTAACAGTTTATTCTACACAAATATTAAAGGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-28 11:09:23 -04:00AEPt@AEPt@APFN \ APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /Z^\)DATA /Z^DATA /Z^DATA /Z^xEDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FWO_GATCGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISP1AMN@P1RLNP1WDNP2AMN P2BANNP2RLNxPBASNNڂPBASNN4PCONNNVPCONNNPDMF\ PDMFPLOCNlPLOCNPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%SCAN@SMED SMLtSMPL[SPACMSPACSPACMSVER4.0SVER |SVER,,5Scal@Scan@TUBEH2Tmpr<UserphARphCH/phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN \ APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /Z^\)DATA /Z^DATA /Z^DATA /Z^xEDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTab%FVoc%FWO_GATCFeat//%GTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISP1AMN@P1RLNP1WDNP2AMN P2BANNP2RLNxPBASNNڂPBASNN4PCONNNVPCONNNPDMF\ PDMFPLOCNlPLOCNPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%SCAN@SMED SMLtSMPL[SPACMSPACSPACMSVER4.0SVER |SVER,,5Scal@Scan@TUBEH2Tmpr<UserphARphCH/phDYbigphQLcphTRphTR,qJ'8f7}7a2&d:Dp$^HA.tHZKo][571\hNQ0J;9 |!$!o%QG{c!%88 =![z)+^[,MZ@Y+P'#:&iChk}`:r&^?HYTw|bT|MI>"J268[`#|D5n4]p%!5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B``,#Xr_<.^ioA+7x,"L-2QvA-zI?I,z|>[mLoCDaD/|8i.bb>~ n3]NE^O{Nf7{hAh.iOWhbk1^wK[*oCf!`LY&'!"CC+}CUtT6S3Nx4Vf1h2sNM(T[ S]T'_C:j|(5,fWz"$b[8)FTk2X'([U)P? s>}J8kOAWm ;op$SqwgE6FM;v&KT_ZO~|Qg\c9UY\LwU`PMm#6mOD5`@CAIxplfwa:t"@>oW~!Eb4;,}|O[nYI.xv^$beP *"udrVLgJRv)1mfc4hAC?-4$&LkfG;7oF`|zvn6&pg;gjmMUwch[}k@_ajFoW~&WZ$>tSEH[!]fKOvx4G!?`T/Hf.X7GtX6|k.j,PCH-p}*4d#R:c@ZIIC"&;UXU8x[K"4#O2C.98xOrz6 ?63_baI23|7hhSc%bVm? S8UXabCDV_mi/fcGHO5QfOl>o %> Ax7cXF$hHzC)OI"v>nh:QV2|x03W *>|{^;5GKXW .C=vz^`ZeW\jhA?I| 9I+IaH]#[,e/G,y"xc_M@a7&^LoT.iFZ8EDlyj,K]yDWYlsDpN$t.D$mOYePjG,F<_ }Ouz x:H-p@~KCozPk&OE6OLiE8nZDKAEPt@AEPt@APFN \ APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtAUDT3B1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /Z^\)DATA /Z^DATA /Z^DATA /Z^xEDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTab%FVoc%FWO_GATCFeat//%GTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISP1AMN@P1RLNP1WDNP2AMN P2BANNP2RLNxPBASNNڂPBASNN4PCONNNVPCONNNPDMF\ PDMFPLOCNlPLOCNPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%SCAN@SMED SMLtSMPL[SPACMSPACSPACMSVER4.0SVER |SVER,,5Scal@Scan@TUBEH2Tmpr<UserphARphCH/phDYbigphQLcphTRphTR