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!!!+++444<=<EEEMMMVUU^]]eeemlltssz{z !!!"""####$$$%%%&&&&'''(((())))***++++,,,,----....////0000011112222333334444555556666677777788888999999999::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a792362ea07f42578235b4496963e98dSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-28_09-06_06723130Seq16716134697_134697mfagan96-Well(K1313_H11_Galianthe_brasiliensis_matK-xFKB_3730_POP7_BDTv3.mobSeqPop7z>;6, &/2.'! 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"$%#!                                                             +31&                                                  $#                                                        /WZ@$ARAGAɆKB.bcpKB_3730_POP7_BDTv3.mobtermGATAAATCTTCTTTGAGAATCTTTTCATTTGATTCTGTATTAGATATACTAATACCCCGCGCTGTCCACCTGGAAATTTTGGTTCAAACCCTTCGTTATTGGGTAAAAGATGCTTCTGCTTTGCATTTATTACGATTATTTTTCCACGAGTATTGGAGTTGGGCTACTCTTAGTGTTACAAGAAAGCTCATTTTGATTTTTCACCAAAAGAAATCAAAGTTTTTTTTCTTATTGGATATTCTCAGCTAGAAATGATTCTTTTAGGTTTCTGCGAAAAATCTCTCGTTTGCGATCAACTCTTTTGGGGCTAATGACGATTGATTTAGGAAAAGGACGCTTGTAAAGCGTTGAAAAGGTTGCGGGTACTCATGCTGTTAGGACCTTCGGATTAGTTGAACAGGAAACAATCGGGTCAAAGAACACGCTTGAGAAGAGGGAAACTATCCGCATGTTCGGTATATTTCTCTGGGTTACGGGAAGAGTTCCTAAATCGGTTCCACCCCATTGATGATGGCCTACGTTCAGGGGGCGATAACCGGGATGGGGGGGGCCAAAGGCAAAAATTGATAAATCTTCTTTGAGAATCTTTTCATTTGATTCTGTATTAGATATACTAATACCCCGCGCTGTCCACCTGGAAATTTTGGTTCAAACCCTTCGTTATTGGGTAAAAGATGCTTCTGCTTTGCATTTATTACGATTATTTTTCCACGAGTATTGGAGTTGGGCTACTCTTAGTGTTACAAGAAAGCTCATTTTGATTTTTCACCAAAAGAAATCAAAGTTTTTTTTCTTATTGGATATTCTCAGCTAGAAATGATTCTTTTAGGTTTCTGCGAAAAATCTCTCGTTTGCGATCAACTCTTTTGGGGCTAATGACGATTGATTTAGGAAAAGGACGCTTGTAAAGCGTTGAAAAGGTTGCGGGTACTCATGCTGTTAGGACCTTCGGATTAGTTGAACAGGAAACAATCGGGTCAAAGAACACGCTTGAGAAGAGGGAAACTATCCGCATGTTCGGTATATTTCTCTGGGTTACGGGAAGAGTTCCTAAATCGGTTCCACCCCATTGATGATGGCCTACGTTCAGGGGGCGATAACCGGGATGGGGGGGGCCAAAGGCAAAAATT%3ES_ep} /<JWcr "/7CKXbny  +7AJVcp}%/;GS_lw  '6?JVbnz "09BNZdo| $0<GR^ku ,<IR`lv    ( 5 > H U a i z  ! , 9 C N Z f p {  " 3 C T b r    # 1 = L ] h u  * : C M Y b l } (0AO`p-BU_r$0>LYhr}(4BL\is}(7CUgv+BLYgv~ (9LYcm}$.:JWev~%7BOXgy#1=LYow!(:EPZcq,;CVao~  ->J[ckw$3>K\dn} %3ES_ep} /<JWcr "/7CKXbny  +7AJVcp}%/;GS_lw  '6?JVbnz "09BNZdo| $0<GR^ku ,<IR`lv    ( 5 > H U a i z  ! , 9 C N Z f p {  " 3 C T b r    # 1 = L ] h u  * : C M Y b l } (0AO`p-BU_r$0>LYhr}(4BL\is}(7CUgv+BLYgv~ (9LYcm}$.:JWev~%7BOXgy#1=LYow!(:EPZcq,;CVao~  ->J[ckw$3>K\dn}   2(+&!/ #,4 /)/*!,/+8;++' ;>8;+3+;;;3& 68.16.688;3;086.;!#6&$  +12;""""  &*+1 &   +!           #                                                                                    2(+&!/ #,4 /)/*!,/+8;++' ;>8;+3+;;;3& 68.16.688;3;086.;!#6&$  +12;""""  &*+1 &   +!           #                                                                                  DU- 4.a=" @ KW\! YB-#bCY;(:HY68NP3% G @\b:) O3'"L TAYE8 DQD,T!=_-3'7P=\JY3FMa`OBA9`H@5>K(E)Rb(EaHQ"UY3*0K&]7$=> .*=KD]3- N(K6$,NPRK 35> R%`/9FO4ZTLP5]')37'6OXI@S#TDIR+I#TPE*S#-H9OSS(W.%1#K USO_Y\ K`` 3VC% BJ !b-ZI >(DF_K=WU%H^N-' PM&`6K+4 AADB)G?@*&:4*^G=^0 =^L&F H& 0:PBcab?GL 0a00AF/*6E>VQW[.*LBK\!BQ'8_Q3A"K4@X3BB7aBZ:.NJRQa0[]^E8&EYB,N>*$\B LS3&EH5>]! 5Nc1H PUX G>5 )2DVYLT 2Sb85\5:-?@<'6O>(%NT[+'c[D&)8Bd@j1aG"jxDG'LTPm{LI7n98a-"-{.*z7zT6 T[P5Ukk6w\|2yNRLM]41 l{.'\`r'g HAQf=q2&^1cNl@T}fpLKm n">3a: L.?+TiOE+ uJe. )|'P'TDJX, \9~R=-RZ\A^- `;+A`%i\+EFH;3.A"aH>{]0 tOr> 1]H`x3q1|cUZN`.Q}K' 6wSOs uj bieZ/E. nXf91g9k'Y@ $;@ R  (p ; 8x}vqxrpVZne]DBUHxiAlQ:72JLg@$0>r32EV_Q-A$:55E<44LD6FIiH/(*C&! IaDDl1HL4sACjE* 2GXrIVH}.B26i`Mh+g/+H@4h9^/TI^dtQ&fmm,741}x(Ct&k5x*6Ft1S  Z("&83IH9&^8`(2H0\EE%+  *>V(9V@zM%0~8 ;!3R)Nj()t8X(p\5#i_ A DR^1-1=*_] A"^,%L6g ;.Ae>+2c19.]t8V#:-$$G3:FPS- 9$q5h\pppO< |w,ppblLpy8Qp8'7d%^i$pIdpWpBoW@_0 3'Sb3 vY;l)hIO+uJDE]/a9)@FdzqNwj)oO?;w#/!v ` C5/  xqfZh/ o8~m)wh?TL d)"8)4%r,:m-1MW,<T1hbqRgWK"8jhTY @{D)-#>GRq}_k=!#n]{{^wr~pr"ydyAdt5Q@yw9_Aj*B^mKjK`JkK!E47ou21H]71l:h |2eJ;XfsJ`m&.A|i `vAlz gP{]MfJ?frbsd~j<UUyo7  S`5WmGW=W vIJ4\tI )@P[yxJ;<j3a&?56 !_.,;`'ed%\!#bpimaqP c *#/;y97Cm9wd R, z;39rg,WD2,7Hsd+ E}a p{ l&x -Lt x.4T4?fr9:xRas ZGGTCATTTATCATCAAAGACTATCAGAACACCATACTCTGTAAGATATAGTCCTACATGTAACGCCCTACAGATTGTTACAATTGGACTCAGAAAAAGATAACCTGGCCGCTACGCCAACGCAACGTGCATAAGGATAATAAAACTACGCTAATACCAGAGATCCGATAATAGGAATGATAGGAGGCCTCTCACCCTTACCCAACAGGGTTAGTGATTGGAAGGAGGCCTCACACAATATACGCATCGGTTCTTATTAGAACAGGACCAAGCCGTGCCCGATGTAGCGGACGAGATCGAATCGAATTATTATTGTGAAGCGTTACATTAGCTGAATATGCCTGTCGAGCGAAGGGGATACTGATTATAGTTATTGTGCCCATTTGAGACCAATTAATTTTTTTGGGGCTATTCTATCCGCGTACGCTGGAGCGGAGTCAGGCTCTATTTGTGTGGGCAATGTCGCTATGTCTGCCTCCTGGAGTGCTTGGGGAACAGCTTGAATTGCAACCTTGACGGCTTCGCAGATTCCTACCGGTTCCAGGTTAACTTAGGGGTTAGGGGCTACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-28 11:10:52 -04:00AEPt@AEPt@APFN \/APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /]^\8DATA /]^DATA /]^DATA /]^xfDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FWO_GATCGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANEQLAST~~JLIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOIS(P1AM5jmP1RL5jP1WD5jP2AM5jP2BA55P2RL5jAPBAS55ِPBAS55[PCON55PCON55PDMF\PDMF?PLOC5j/PLOC5jPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N% SCAN@SMED SMLtSMPL))[SPACMSPAC8SPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEH11Tmpr<UserphARphCHVphDYbigphQLcphTRphTR"wThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN \/APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /]^\8DATA /]^DATA /]^DATA /]^xfDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTab-FVocFWO_GATCFeat..GTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANEQLAST~~JLIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOIS(P1AM5jmP1RL5jP1WD5jP2AM5jP2BA55P2RL5jAPBAS55ِPBAS55[PCON55PCON55PDMF\PDMF?PLOC5j/PLOC5jPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N% SCAN@SMED SMLtSMPL))[SPACMSPAC8SPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEH11Tmpr<UserphARphCHVphDYbigphQLcphTRphTRk&zt Mi3rs{6`V5r@AKl&FaewMZ>fcn.=[|ebhktH4*&qve4q,8kf RCjxeCW?R5Ch2d'jw~|>J3zFReL#(78WFoe~8/zz-nX>z3KQv3.5s\yC!}WQ`Q5f`lbkF3(jY`VqQIxwcL6-*pfFUeiM/+U*yxT/4f!Z56N'?k?~pmxRVQQ$zWkp$=RyS4*7rtlq8&LabDvv'O` JECce3YXjld96"$M@>b|U2mC!KCo6T*-dotq$C9|f|Qc^F"P95Li2R~5OPh~jThh@<}9W#()P0&Mqzva].p>x'[vA-\K"L1{!Ul62dl=?o'%:`Kle8h/_Dea_';_/d@ikjFDfqcd o;X.>S[jWcgnW6Ob#7!VkW4tK2qi|j#_Fs4)E;S|DzUC`k@l'Z9dgTiK]j;K.LzeDW[Zeh&f&{-'_1k=`<{!s73|?j~J!Eb4;,*`S]8a,D:rF(5,fjG,F<_Uu!N&e+{wL6i23m.Oc:B@g]uB9*!K#Z]Pk=o?eU-:.<)%,8#V>Ax`X~lTmt.qK|-o*.AQ>UA!%EeKOWQ`QV-!w2l89+uTE#!#hmmfc4hAo?e3{0+oiw87[%Vr6R[Ule,rS]RnjH,3LiPtj glX ,cW`}F)h>?[ha_DMOFU>^[?|&Rv3672Z)~6bI.J:*yEwiOM#Y\V&&OE6OLogXY@ZB 3O{ ~VJ(/on}sF{AR8=(V-OGC#!^MrrG]#Q.faX1"Qe-1^X.w)C~W)q*Y`Z8SwcI^zI[y+=Ht+a,|&bX*F8Ba+b8EYWC.%oKO> `VsIefaqQl&A6PG>}Oujd+[Y.m/wBN $`?"*J[x5FaGwpBT9\qwH=qd)B*vH%lF1?HOeCvpN^3jNRjnMgx*&`R@){wJUo6X?/WZ#&a:OcP$SXtmx+9Hgn<_swW^$buJrUlM;_}$A>e6bZ( iu2Zv&1`Z=WcDArh]|Ko5qS+SP7T]TcSI*$6ey(B~bea6:!.tk}Y=52rR~o[k7MLr92U*a!