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"      #$" /# #+""  jd,,,III^^^{{{&&&///DDDNNNaaavkv      ***333>>>GGGGGGRGGGGGGRGRGRRGGGRRRRRGRRRGRRRRGGRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRR;SSSKKKiii !!!+++444<=<EEEMMMVUU^]]eeemlltssz{z !!!"""####$$$%%%&&&&'''(((())))***++++,,,,----....////0000011112222333334444555556666677777788888999999999::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 3924eeda37344e3dbf7ad0cccb6ce42bSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-28_09-06_06723130Seq16716134697_134697mfagan96-WellK1313_A3_Setaria_sp_matK-xFKB_3730_POP7_BDTv3.mobSeqPop7zV4HD1"Jr(6\i 1@EOi%r ! ? 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'+                        #           %    $ !)*+'$ * ++ -+ "'+-3+++3"" '".'. +  # """# +   *++#+                                                                         -                &&   *  !    '+                        #        &6:[ $$$DQP)"M^ES.cUB0IF.^GQ67 O\KB bV`<Z-[3H<W=JP\( 7cBZZc/5%GN`E M,)>&X, 0T[6: M.=/0#>-O:) ^_/6A= PJ&W[.Db ^])'aS2W_JG]!CMN 2Z'c@1B6U%+<1'>0:1BP[E0OCT9AX_CLW[\"X8GQ%IH6 _Y[)C!X: 8TILLP4 : 5S 'T#(X0-S 14KY8G)F3=Y G6% 8G:aKG >%!< F b7D#Y"\2X&O)>N VH6b>a$+ $aR\X4P8L\IJ$d1(9*T:H2R?U[],Q[>_4=5a?4D8= +G-=BZ)U0>Jd<&?5/O,PK_#:O574IWM= !;=E'3?'S .Z/>IK;>5 ^HRcG+U#c&=(;;+2^3 O3 Y^[ Y2 C!(D_3SB -_ ,VO5\I7$bO=PN1T >PO=!TPF<2@5S;@$6Z3>\a2`(\K:*8OFCPBOa24XM !2+O L)d\)ZJNW-$Z5Y,QB5V>&Z+,G=.A7-Z,8d2\NDL?2"XW 'AZ2%DWZ5]@Zc_A _$B=940"Y"@IR$!\7R-G8P2ZZ R7I-=I, .?Fy/ 2 F'+}f vU1#6d Y[ ? ^Jm&?1i;&e(^) >p;MjUe\csajOB;R z@J>z@B*l~#C  3 c/`US-!-\&s.q(H."tcPW-NO:IV.66=nNW bQzY^L"s@tL91^mWvp(+?xVQ =\ A559H/GY|et's:1dZoQeE"Kp9}"QOi-s\7N$EbtXe"26\^r\R/.CyC=2TdKOR &{ \ 1.ARXOb}_@dT&I?@zjX c?n\gC7qpY9dDoFqz]lRa2}=oH-:#%Jc?  e};s&dl}>s/ q!q ~<` }_;Dp5 5 )hp Ph`nv!QvywkqSm*fC}@omHGKTcFJlsP6+^b`=6FPSXrA]A?+' &Z:+>4-Sto+J,R`N-8 ><,?- >>3D9s"^|i9F9<P[\egC`uLqJs{[Lecm $AY"Da@coT\ojR kmT}r3x {wMbd\G+d65=YL{z c VD}Yot8mdi~N,eIeMx,dwSV&j+U3~LwRfd ,KYg l:$Rs3K0NK"Ur4H_|=IK c.MLbU85..t(GW'CEsSCMYYvC(.DOD&@+xHD|-UnWu  777 77 777     7 77       77 77 7    7fffffff)fffsSKSfSSSff$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$6Xn66Xn66n66nnn6XXXn6n6666Xn66X6666XX6nnn66AXnn6666666666n6KnnX6Xnn66686nnnnn6Xn6666X66696nnnnnnn66666n6666666X6XBndXpp83ap;_i'Fp[Fof{d|*&_/ hpWnLp-#vp0@T*!i;8OCHJNgp4 ak[c Drr{Cp@)|A'1[2z+6zT6Y1S!L%yn"A11*z28Br86Q$o[g;}BkeX6k,%dg De Z;<[Do .#x&` _:+sI\lp\pj9XW1aNBppq,#Z.'pd"A  >1 o J Z[@fMHeH > { kv<8".ge60[^(*1)'\Pmjr()k)@Z=3"'s[;H*$\WT jR1VTX^KH+}, i2P=^5))JIfM tP;7_JtNtIB+8CoLO;U:E PFuNKX[}^1JqT:hY@yb hpm,5 ~wZR ~?9K1jY,u7;j7liI;E%nZqB] M+le ]M3&LS<H\S (M{H^:t'H`zK41vqK&9d Mr  BchBy,ECf,?~`LI;P<.jlBY4.H8W d`SnOf/= "W8 ,H~$#UbN&V]DrQcm](X*[2X8%[2-AAATTTATCTCTTTATTATACTGAATAACATTATATAACTATCGTATAGTCATTTCATGGATTATGAACACATTTTTAAACTTGATTGTCGAAAACTATTAAATAAACGTTATGGTACATAAAAGCCCATAACGAGAAGCCCTCTCTTCTATTAATAGTAGTACTATTGTCGTAGCACCACTAACTTAGTTAACCAACACCTGTTTGAGTTAGGCTACTAACGTTTAATTATATCACTCTATGTTGGTATATGTGCCCCGTGTGCTCAGTTCCAGATATCGCCACAGTATCCATCCCTACTCCTCAAGAATTGCTAGTTAGGAAAGCTAGCAATGTTGCAGAATAAGCCATTCATTAAGCTAAATAATCTATTAATTCGAATCTTTTAAAGTCTGCTTCGAACCTCTCCGAGAGGGGGAAGTATCCGGTTCACTCTGCTTACTCCCCCATAGTTCCAACTCATAGCGCTCACTATCCCTCTACTCGTACTGTAATCGACACTTGGGCATCACTCGTAGTGGAGTTCGTACGTAGAGTCTCGCCTTTAGTACTCTATTTGGGGTACGATTAACCTCTTGCGCTTAGTCGTCTATTGTAATACGGGCCACTAAAGTTTAGTATTGACGACTTTTATGATCCATGGCGTGAGATGATAAGGTGTCAGATACTCTGAGCGGGGTTTGTGAGTTAGTCTCAGCCAAATAATGTGAGTTCTCGGTGCCAAGACGGTAATAGCTGTTTTAATCAGACAACCGTAAGGAGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-28 11:09:57 -04:00AEPt@AEPt@APFN \"APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /_\+DATA /_/DATA /_3DATA /_y7DCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FWO_GATCGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISCP1AMDP1RLNP1WD:P2AM&P2BAP2RL0PBASqPBASvPCONSPCONXPDMF\ PDMFZPLOCbPLOClPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%;SCAN@SMED SMLtSMPL[SPACMSPACSSPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEA3Tmpr<UserphARphCHqphDYbigphQLcphTRphTR;This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN \"APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /_\+DATA /_/DATA /_3DATA /_y7DCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTab FVoc FWO_GATCFeat.. GTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISCP1AMDP1RLNP1WD:P2AM&P2BAP2RL0PBASqPBASvPCONSPCONXPDMF\ PDMFZPLOCbPLOClPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%;SCAN@SMED SMLtSMPL[SPACMSPACSSPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEA3Tmpr<UserphARphCHqphDYbigphQLcphTRphTR'%N`!2FPu4WyYZ(Wa:nuXK|+U17ujZj_!p-">R2`Yyb oJH.`Ep2=TgVSQ<}LgU!IZHp#)+KT[^[G^%E:"*l#qN8>o Ueh,9n5NJw=;SocJyw*6TdO{7I{4^PrtC[J19D5mr+8B9.~;{lUz{19se0:=y=&e=1&!rEV(V-IAG&e"i/ud?7OBmxCB#v6|k.j H_H-CI;{dY7cI*]5cP}Gs+OStxHlYxC?(cU/~!-,'bF4$T9tR3#=cC0^,!Sk2%vAfIx+C!K4#]BN $`?"*JIf7Qv6,MZ@Y+P'#9r??8r]f.iD4ZI,|Q|T{\K71P3;fTR[fRr;-6U@;EGCfQ&HQ$ 0603XXbLmX;9 |.W162Q$OEHO6z7B-_8Es{sMV^@}Ouxg>33.BkST{!Xf] MzQd)l'[)VBe$O!&HoCdNmbcnPn&p;s_|g+XfT;KB{s2fo;LY&03t0mP`k:!.tkj^4E2s JX0bt-] 5y$47y 4orKOR`{A,}@1%i`+|*#pB6G}jgEqv)m&WTur&YNA,zFYLUE3Sk-[qr[&X1EDE?]mnjH[$.}RZ#3|5O-U[d1DFW%j'kT'_C:Or4^7~s?K90lVSFsA bXX<,x'EEwiOfFWcDArh]|Ko5qS+SP7T]Tcr[&$&?_1OPh~jTn/])RpikE:b&b)>`#6E12 d+[Y5xo|[EG\}d hfz6i8Z26bb+i/_wg=A#Q9nPk@_[O~}<"&eG1h3/[ze~!Tq*nEg8Z~d_1{63_iGbN+7F= E"ae-F*r`s#T)3KO]h^* `v?{}I8w$YWmOyl}$rv/qwnF){xD\4AFUTT3T&^%FyPC,_,qz7A*k|_LhvHZ&}IgspSHSM~$w?D2UQLY7n nk0WC.%obB`!3<-,RIlVy8J9UTBe$-s:goT|0\d&POhAFDHYWm;2FVDp,CD4;,x8yT,I)rw&^LoT.i792C|5( J!Eb4;,}&v-T)D@4ir$GG\}d Go: