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     )))322;;:CCCKKKSSS[[[bbgjjpqpwww}}}a<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 0802c56862f64d7bb9766d8e9a6c98e2Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-20_23-24_06283130Seq16643134538_134538mfagan96-Well*K1313_G3_Smallanthus_maculatus_matK-MALP-RKB_3730_POP7_BDTv3.mobSeqPop7z-6:=FYx5J^y/AMW`hpux~W,xY8ziWF6( 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"%(*-28>BDC?:6569<>=:74200.,*'&$$#"!        u@YA5ATAKB.bcpKB_3730_POP7_BDTv3.mobtermTATAAAAACGTTGTTTTTTGAGATCCACTATGAAATGAGGAAGATTCTGTATATACGGCCAAGCGCTCAATAATATCAGAATCTGATAAATCGGCCCAATCGCCTTACCAATAGGATGCCCCAATGCGTTACAAAATTTCGATTTAGCCAATGATCCAACCAGAGGCATAATTGGAACAATACTATCAAACTTCTTGATAGCATTATCGATTAAAAATGTATTTTCTAGCATTTGACCGCGTACCATTGAAGGCTTTAGCCGCACACTTGAACGATAACCCAGAAAGTCAAGGGAATGATTGGATAATTGATTTATATTTATATAAATCCTTCCTGGTTGAGGCCACAGGTAAAAATAAGATTTCCAAAAATTGACAAAGTAATATTTCCATTTATTCATCAAAGAAACGTCCCTTTTGAAGCAAGATTTGATTTTCCTTGATACCTAACATAATGCATGAAGATCTTTGAATAACCATAAATTCGCTTGAAAAGCCCCGGCAAAGACTTCTGCAAATACTTTATTTTCCATAGAAATATATTCGTTCATAAGGGATCCAGAAGATGTTGATCGTAAGTGAGAGATGGTTACGGAGAAAGAGGAAGCAGATTCATATCACATACTAGAGTATATGGAGAGATAGTCTGTGATTTCTTTTGAAAAGAGACTGGCTTTCTTGATTTGAGTATAGACTATCCAATATGACACTCATGGAGAAGATCTAATAATGCAAGAGAGCATCTTTATCCATAGCGAGAGCTGACAGATTCAGATGACTGGGTAGTATAGTATATCTATACATATAATGTGAAGTGTCCTCTAAAAAGAAATGGATGATGTCGGTAATATATGTATATAAAAACGTTGTTTTTTGAGATCCACTATGAAATGAGGAAGATTCTGTATATACGGCCAAGCGCTCAATAATATCAGAATCTGATAAATCGGCCCAATCGCCTTACCAATAGGATGCCCCAATGCGTTACAAAATTTCGATTTAGCCAATGATCCAACCAGAGGCATAATTGGAACAATACTATCAAACTTCTTGATAGCATTATCGATTAAAAATGTATTTTCTAGCATTTGACCGCGTACCATTGAAGGCTTTAGCCGCACACTTGAACGATAACCCAGAAAGTCAAGGGAATGATTGGATAATTGATTTATATTTATATAAATCCTTCCTGGTTGAGGCCACAGGTAAAAATAAGATTTCCAAAAATTGACAAAGTAATATTTCCATTTATTCATCAAAGAAACGTCCCTTTTGAAGCAAGATTTGATTTTCCTTGATACCTAACATAATGCATGAAGATCTTTGAATAACCATAAATTCGCTTGAAAAGCCCCGGCAAAGACTTCTGCAAATACTTTATTTTCCATAGAAATATATTCGTTCATAAGGGATCCAGAAGATGTTGATCGTAAGTGAGAGATGGTTACGGAGAAAGAGGAAGCAGATTCATATCACATACTAGAGTATATGGAGAGATAGTCTGTGATTTCTTTTGAAAAGAGACTGGCTTTCTTGATTTGAGTATAGACTATCCAATATGACACTCATGGAGAAGATCTAATAATGCAAGAGAGCATCTTTATCCATAGCGAGAGCTGACAGATTCAGATGACTGGGTAGTATAGTATATCTATACATATAATGTGAAGTGTCCTCTAAAAAGAAATGGATGATGTCGGTAATATATGTA);GQ]o!2=CQ[bnz"-8IVco&0=FR^gr~ &.:EOXer  +4=ISamx#-6BNWamy'2:EP\es} )2>IS^iu   ) 6 @ J W d n v  ! , 8 B L T ` k x   # . : B J V b l x   ! * 4 ? 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"   ($$#+!   % !             .    *! )           ,      -#L@]B8*JB9N H>7d>N=!=M  ,0;S H1a:QG "Y+HY ?0&;HW$T0J.N_" CG>G 7FDW(!WL5/*]K+ ?=V<7\>]0&DVb 8;'.A<b M a42P; CJ-&9#UL/-;TE+^G MP@44%27224[4PDZA^>! K^9bU% 'aW$<J/= .<c![2 CF8QK/%Q"D!#9#?US[,,7;E^RQ&ZbN92+ Y0bCc FO/ NF##AV5[-27DJ[-7S3-7[c0P`<9"='B>DA5D(Z95FM]a1EVC. 2Ad7)@$- @!P--cJ2.)(WJA#c13Aaa c2<<L_66LD<'C(<L+9+9$>Z2 ( W<$+97#+922)M`?(/5<ZC]/&@ .695ZK7%F(]U52/.6.6!CD<.2655 .(L_4S0 'P=K40=E0]7420H22Q2NQI 82A2#L+O]RAVA#04`#G*1U2D.8a!('*"9A QM0-KC ES2E85;_>E+2FZ 22F]?<T0/!;W QA2b6$JJ9Z- 2W@ GW9b'F;,JNWH[SYNTHJ2P/#UM H2G"2)2U.R0#22^&b^:T\74P&>Y(EYK=_%7!-`N&8-K[2<22D 7-&-F`2E+HJ-R:a742O%%2_D`X7-;XI[DS]7_A#K-ML >2 ``]7]DEM- -OGZsKWB EzDtA8.qO}R-;?#q'S(HFKnD)yms=M$+!nb@kiC](\j+ -b~O2,Wy&3q>G-Hp;oDiLqhKk#'z=?*F,D'3+VE]*=~V7`AqaJf9mot]/Dv8YqQ!)VUmR [Ow*vc5~wgL)'~ Bo# e~>4Z p+a&r!y&z%iwUV\6@`2jD1  )p'0Y`GEgBJOTR'-<ht&62yV9l<<0hHF=lc.TS'=FMnab$l,1sc'(<B JM!9$v5\azQvo>H/uZ!YyTMBjtr8t938icDy"rt\x=8Jywz y_DHB.+rE7U>YFd>|w.}`)Dgh  m- 9%PY(  Ssv@2; 9tk h G4 H{~oc 4m;^ 6x\fG{%TxwwF[VsgaW?bJ3<aoC\9XO,mfmAT^T[j#^B@G\bE;,,8A;==-4:=2Jz*B^HB4KB/4)=[;76&$1=CJFWA3C7?9&8O>G<sg?hXdYadzXY3xGWL;CI48pUW>QWRCVNGl1YC+"HAebk2BUcMMMMMw5^NdZwoFC^y\TV\o/yY&`UVV[eee)Rcc`iua4cBjN*exccc]gYYYtRYYY___YYLZTii{diik\gpciiifiwwUlld~llimim~~uxxxmmUO\SxmxxxxnLjRcQW N-CCCC<  <<<<<G\<<< CCC CCSCCpC-V^;^;j;^V((^(^^(^jj^^^^j ^^(^(l(ajjjj^jiiYTiT BipTiTZT~TiTTTTTedTTTTiiiTTTT`TiTT Ti#1:iCp5Vz; dJ2hibJvppod3\tR7pJJ8m$p4JpH)ly;pwzVG  pl\Bp dp01mpp6UGpN,pp(o[] pfpWOp>p5pppppGC,m,ppppFppppp8$(,p_e!_M<$8  <arvV'*IT -G%_D 3quf ji-!iH|%xunwUv^],q.]\()8n0~A&ZYY9Ip:.92B\-cP`=p)kczCy<Io"lOtkWp"=0] [Rqq\dsIyJNrYl3xLmf\#et1ahR1 J Ys:Zb?V/yJy0",9^;uTIAUR$N_n'4k0+B'5sX5]!o m-qS('r/~<RG) sZ(&0I_W:u^cyT1 < s{Eju Tzx)yy|R /^UJML4nM(^K35Rga^\{J>hA#JO:f wBefEZyLY hEpL  7wS\|huBC vwU,iskSI)Z+;885|lRP.v0>HC||(H(RTLEC|%xF;k sQFkAATATTTCCTTGGTGCAAGGAGATAGACTGGGATTGGATTATTTTCCAGAAGCGCGGCCGATGCGCTAACTGTTATAAGTTTATCACATGTGTCCGGAGTAAAGAAGCTTCTCGTATACGAAACTCCGTAATACTTTACCTTTAGAGCGACTACTAGACCAACCGAAATATTAGCAGCTTTAGAATAGCCCAACTCACTATATTAATAAATAATCGGTATGTAACCTCCCATACGAGAACGTCCAATAGTTGACGCCATCGGCGTACTCGCCTTTTATCGGACAGGCTGTTGTCTGTATTACCATATTAAATAACTATGAATATATTTAGTCCAACATGAAGACGACAGATGTTTTTATTATACGGACGGGTACAGAGCGTGGTATACCTACACATAAATAAGGTAGTCATGGGTCCCCAGGCGCGAGAAGTTAAGCTTCAAGACATCTCATATTACGTAAGGAGATCAGAGTATCATCATGTAATCTCGAGCTGCGTAGCCGCTGACACCTCCGGTTACACAATAAACTACAGAGCTATATACGTGCGTATGAAATATACAGTATATGAATAGTGTGTGAGACATAATGACACAGAGTGAGAAGGCGCATACTTATAACACATAAGAGTGTATACAGAGAGAGTGTCTGATAACTCCCAAGGGGAGAGTCTCTCGCTAGAGAAGAGTGTATACACTATTCTATAACACTCTTATAGAGTATATCTTATTACGGGAGCGCGTCTCAATATTTATCGAGAGATCACACAGACTGAGAAGTCTTTGGTGTATTGTATATCTACATATATTATGAGGTGTGTTCTCTGGGGAGTTATATAAGAAGTCCATTATATAAATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-21 01:11:33 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtcASPtcB1PtbB1PtbBCTSBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 0`TDATA 0`DATA 0`DATA 0`tDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~dLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISԼP1AMWUP1RLWP1WDWP2AMW _P2BAWW P2RLWPBASWWFPBASWWPCONWWPPCONWWPDMFT|PDMFPLOCWKPLOCW۝PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT $RUNT  RUNT / RUNT  Rate 0RunN""J:S/N%ԴSCAN@SMED SMLtSMPL++TQSPACMSPACSPACMSVER4.0SVER SVER,,/Scal@Scan@TUBEG3Tmpr<UserphARphCHphDYbigphQLcphTRphTR4This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtcASPtcB1PtbB1PtbBCTSBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 0`TDATA 0`DATA 0`DATA 0`tDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab$HFVoc$7FWO_GATCFeat//$GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~dLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISԼP1AMWUP1RLWP1WDWP2AMW _P2BAWW P2RLWPBASWWFPBASWWPCONWWPPCONWWPDMFT|PDMFPLOCWKPLOCW۝PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT $RUNT  RUNT / RUNT  Rate 0RunN""J:S/N%ԴSCAN@SMED SMLtSMPL++TQSPACMSPACSPACMSVER4.0SVER SVER,,/Scal@Scan@TUBEG3Tmpr<UserphARphCHphDYbigphQLcphTRphTRMr,c],~:L< zFefcXM7WX}lna] 5EX6R2? Cq4GPQFG&908]/eJ!`WBV*O.:U q<6(mu y-WPv8UUCEDFi3V[,A4#OvRio5z{,'~E{}P.Ea}_eaejp~0,{VBtn&gqr;zuhGlB5-S6C,nJ3qo8Mew.KzQ&:C=oq#g%rl(Lii$sL9,X/5`w=yBimJEIoM/V,<|ag&<{[;N }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[Xd:< dG#$w17h+eJ/n=Y?iQ7wDUfS*rR_V?7Z(IIvU9}cw]|Abe KR<(Ks-( ,J~V4F*0(W*KH$<\![2iPS!T/RD8z{&]t[XSXW .CANq~Q9U,Z:X=<;'4"C\|J_@A[]"TM,s"6rg8Z~9}__';_/x"ieGlhx6m}PzN?29zxhW)!hs|*>H~?p MD8zEXTMk|k.j H5Xv&~^\mcJT&1o?e3{0+VNwBx6Ks;6R&8yT,I}_7~j{nF?/aOw2gpO0,cW`GCYHs@dUa0u:W^$)P? sI}}1%j-]Tsz^-d+O'i~88u h`k@_[O~}<"&eG1<@O(LxCmqL6&pg;gjmMUwch[}k@_ajF= =&e=.7I 9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,I~<'",s@3[{R9Ya)@Xp}"aFV;LX4KU>?+Nhsr5%NH!&$zE){oUWx6YlkQ7}^y=nb*{eWf%_^&F5KKs0.u/m}s6)\R5y=Y`g%gcmea_';_/e"^=0UA!%EeKOWQ`QV-@[3>>B)u:[owQC(vJnPE*F;2_/gYFi$t_SF`|zvn=Y`1&!AuJUo6;^F6@_=!)}W}oo-o;X^`>EY$y!TW3nG6`VX+*fl{iG>A.8X:B?r@i$!^h{we**,ACT;fQGE-`x&i)6JWT|*0_fs$X=<;#(+gbk1^>7(FTcSO[mOEW<#GnP Qc$@)k,g5?wAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtcASPtcAUDT2[B1PtbB1PtbBCTSBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 0`TDATA 0`DATA 0`DATA 0`tDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab$HFVoc$7FWO_GATCFeat//$GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~dLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISԼP1AMWUP1RLWP1WDWP2AMW _P2BAWW P2RLWPBASWWFPBASWWPCONWWPPCONWWPDMFT|PDMFPLOCWKPLOCW۝PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT $RUNT  RUNT / RUNT  Rate 0RunN""J:S/N%ԴSCAN@SMED SMLtSMPL++TQSPACMSPACSPACMSVER4.0SVER SVER,,/Scal@Scan@TUBEG3Tmpr<UserphARphCHphDYbigphQLcphTRphTR