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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRPRRKKKiii      )))322;;:CCCKKKSSS[[[bbgjjpqpwww}}}a<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5f135af6ba44493ab8dbf2de7a676da5Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-20_23-24_06283130Seq16643134538_134538mfagan96-Well K1313_B3_Paspalum_sp_matK-MALP-RKB_3730_POP7_BDTv3.mobSeqPop7znv;d"Eh>^v9Q^a^XUV\clt|ymb\[alx{m[J>753.$V0rGfD ~aD%wne^VPJFFJR]gnt{ +P{)d6Vo}zocWH2\7|L hP@:=FSct2bV 4:2Ju83X$8<0i<=!MT@bifqn>  #,6AKU`ku~{paRF=99;>@BCCCBBCFLS[cimnonnmmnnlg^RC6+&'-6?JWi'[=h )TvM4-6OwPw7EU/ $+.,%%@jb^Jn}@b,Lt-f8 #,9Mfq98d{}oU+L7 P<I?C^2 /@P_iopjaVLECEJPUWUOG=4-'"  .h4Z|^%IQ<`zoZndB2,.5AVug;oO(k0 %Gw DO>_2   :j1e}\$]0 (a,>IIYG^1aVmo[~N T/ @h}Fla(TZM *c0o3ma7 T&   .SCf m: '/59973-(#  0U4C@, S/.Z"cV[2 %,5>FMPOIA80+)'$     -Vr';mrAQ(t_r Q-$IMUqd3(F )6BJNNIA7,!    5JAV;yF#   $'())(%!1WO`{f%RI%)Gy U@k[vuJU( )c{y&D+/R)    !R dz"8~J/'(.9Mm7V_N(V6# #%&(+06>EKPRTTSPJB:3/..+# ?{Qxh7q>  #-?_SlgmuidWa)*`; "@j"[{P[3 2j ]hI! 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'2ATijS?/$%0=JU^dd`XMA4'  "4Jaw{m]M=.  -?Sfyp_OC<:>FP\hry~lVA/  /JlwX>* !*8HXfpuurlfbadjs|~sbO<,  "3FXgt}xmaWRQV]dgf`UI<1&  )>Ti{uaM9($.;IXgv}l\NECGQ]jsxul]K7%  "4GZhrurld]YX[bipsrlbUF8*  $6HXelmiaVK@7.&   *0330+&   #*047863-&                     "#"                                                                                            @AA^A=w2A]KB.bcpKB_3730_POP7_BDTv3.mobtermGGGCAATGACTAGATAGTGAGCTGACTCGTGTCTAGATCATCGCGACTTTTGCGTGCACGTTTCCCTGGTTGAGACTTTTCTGAATCTATCTTTGGATTCCTTGTTTGAGGTATTGTCCGTTCAAGATCCATGAGAGCTTTGTCGAGCTTTCGTCATCATTGACGTTACCATCGACCACAAGTAGGAAGATGGTGAGTTGCTGCTACCTCCATCCTGGACAGAGCTCCCATTGATCTTTAATGAAACAGACTTAATGCAATAATTCTCATGGATACATTCAGATATTAACATTCGCAGAAGTACGTGCACATTAGTATCGCGATTCCGTATGACAGGAGTTATGCTGCGTTTTACTTAAGCACTGCGGTCTGTACACACCTACATTTTCGTGTGTTCTTCATCAAAAGAAGGAGGATCCCTTTGAAACCAGAAGTGCGTTTTTCTTTTGTATTTCCTTAACATAATGCATGAAGGGATCCGGTGAAACAGTACATAGAGGTAGAGCCGCGAAATCCTCTCTCGACGACTTTTTACAAGACGTTCTTTTTTTTTCCATAAAAAAGAGAAGTCCGTTCAAGAAAGGATTACGGAAAGGAGGGCAATGACTAGATAGTGAGCTGACTCGTGTCTAGATCATCGCGACTTTTGCGTGCACGTTTCCCTGGTTGAGACTTTTCTGAATCTATCTTTGGATTCCTTGTTTGAGGTATTGTCCGTTCAAGATCCATGAGAGCTTTGTCGAGCTTTCGTCATCATTGACGTTACCATCGACCACAAGTAGGAAGATGGTGAGTTGCTGCTACCTCCATCCTGGACAGAGCTCCCATTGATCTTTAATGAAACAGACTTAATGCAATAATTCTCATGGATACATTCAGATATTAACATTCGCAGAAGTACGTGCACATTAGTATCGCGATTCCGTATGACAGGAGTTATGCTGCGTTTTACTTAAGCACTGCGGTCTGTACACACCTACATTTTCGTGTGTTCTTCATCAAAAGAAGGAGGATCCCTTTGAAACCAGAAGTGCGTTTTTCTTTTGTATTTCCTTAACATAATGCATGAAGGGATCCGGTGAAACAGTACATAGAGGTAGAGCCGCGAAATCCTCTCTCGACGACTTTTTACAAGACGTTCTTTTTTTTTCCATAAAAAAGAGAAGTCCGTTCAAGAAAGGATTACGGAAAGGA-5?IR_j ,9DN^ht ".5BNYes{#1<HTakx #-:FT`o} %1>HS]iw$/<JVblw(6@LWboz0=FQ`kt   - = K W d o }    & 3 ? K W c o z   " . ; G R ^ j w    ' 5 @ M [ e q }    ) 5 C N X f r  +:DOYgs $2:HVco|*6CJW`my*7AR^jw(6DR^kw +5@IS`ly!-:GU`o}$,5?JV]gny"1FOZeq~ "0;JVemy "1=CN[fu} )3BP]hx!1=IUamy,:HVcnw-5?IR_j ,9DN^ht ".5BNYes{#1<HTakx #-:FT`o} %1>HS]iw$/<JVblw(6@LWboz0=FQ`kt   - = K W d o }    & 3 ? K W c o z   " . ; G R ^ j w    ' 5 @ M [ e q }    ) 5 C N X f r  +:DOYgs $2:HVco|*6CJW`my*7AR^jw(6DR^kw +5@IS`ly!-:GU`o}$,5?JV]gny"1FOZeq~ "0;JVemy "1=CN[fu} )3BP]hx!1=IUamy,:HVcnw                              #                                              '           "    )%)':   #  ' %2(     '(    # 4!       *! "   1 "( //(!                                      #                                              '           "    )%)':   #  ' %2(     '(    # 4!       *! "   1 "( //(!        7D F53.V0Ha9%,E >$"#Pd")DL' YAO4!@E3L=?K5VTG+96B"6,KK;=#.`(N=(!['DI-BW`H55!ZFFI-; cIaLcGJD^Z#O%82c<# #9;M>U  1'%F@Q:%B)?,8-_>0B`cBIa::HO*F\1S-[>C>=.N<XG;]+ZO$WY7.K[2@<.5+:=:N5LS!&,HCT;!CR+OUL^Z ,6N9M0I53Z C Wc)22T /G"NTEB6O/RDIbP#_2=@`PF"U_JO'2OJ22/ 4^3U2,XO 92;F !QM5=-HHF[MB%8< K' N<"#^A'![LJ22c.R2BI6O )P)JS*".26=%6WDEY*ACY2!d_!^7'Ycc1#22VY!22d!,82W]4#22V)P_P Y,9K*`22HW6.%#VY9*8@;^0O/\G22UO#319\$2@O]R*KM$*/5:]EEEC D2IF2E+2AH,8E+< Fq5A)NiGvBytC]:m!A!A+u\p%$-v'>~a<{sW\?H [?+|m@f{DIiuw55U"CAO3=P\U;,  AocmS r-Z fhhBM4 2P3`?J1w`t*Q>E -SEA^l!M+zI55 DZs BN#X*)Yz<>MB*x-1z&H!AbK<=Kg&0fD 5 P[N{P& 4 f)<~*zaP0gE  !!Z !?@cBqRIF@ B!n ?"DCOy9{,4|Tct_TY2Z xuOu+R| Ue8OAd%}X}i/rpmdzR1~_GR L5 V&<Wq72*-*{/[UgytdYmmBsqSPyjDXiI`EgVzJ>@,.OR?Ai19T{J!E 0B 'B@f)@N6)IJ<`97Y8S8MTCfh_KIH=!.(53Q3?uM4  1+4()Suq<K'(c=YC]GSE,P%DB+D(@74LF>>;g>>>e>=]M"">>3BI>>"Y>""e&`>"yT0"?P_""pGXEO-8^SpQpet%O^Yh_TcpSOT^O{f{mvodrmpmmzzcm{z^}}gxq{uwvttundst`u@Qrt`~rQivvvvvvnnnntvvvvsn vPzg 6p,2vl,s82`)JSywMOBg6 jf>`zw+rX-EhLGRzN6 Oj 2=iqe/`5,!_"#YuAmw@!LW8((L8L1-CQ_m D?k8IWr+ AMpp  tp&Wpppj?r Dp8>9|@,B^E,>piCpoT8@JFV|f3pp$f2F7E,tb<UiD+ h?NLcSQg|qWu`9AcgF9CVhk}XnldWnknMh0t'>Cmc^< j;gy\*"dg3>'?4oRa8R]o u 1E07*U8r04+z[W$2ap/wg?j/nxUPs H$#OsHNnwSRyuw ,Qo. *|ds _]ntO`^3fMeO$6mk1{k`_ 02(Q.md$NnP$lsQQ1<]-nnmI;%8.~P*[:RkZ O,XA]tu!9=6v4~TGui|s~,6fqc?xZtA*j!OGT=CCCGCGGTCAAGATATAACCCGATTTCTCCTGAATCGGATAGCGTTAACGAAGTGAGCAAAACTTGCTCGAATTTTAAAAAAACCAGAGGGCAGAAGGATTCCTGCATTAAACCGTATTAGGAGGATCTACGCGAGAAACACCTCTAAAGGATGTTGGTGCATTAACGGTTGATTATTTTTAAGATCCTCCCCCTTCACTAAAAAGAAGAATGATCCAGGGATTTATTACCGAGAAAGGCGCAGTCATTTTCACTAAACGGGTACTCTTAACTCTGTGGTTTGACAAGTATCAGCAGCGGAATTTGATCACCATAAGATCGCTAGGATCCATCAGCAGTAATACGGTTCCAAACAAGTTCTCAATGCTCAAAGCTGTCTAGCGTCACATTCAAAAATCCTTAACGGGAGGAATTTGCTTTCCGTGGGGAGAGTTGCGCAAAACTCCCGTGTAACTTCATCACCTTGTGGGAGGAAAGCTGCTGTGGCTTAACTGGGTGCCGGACCGATGAGGTCTACTCGCGCCTCCGCCACACAGGAGGCGCTCAAGCCGGCTTTATTTGGGTGAGGAGTGCGCGGGAGGGATCCACACAGGGAACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-21 01:11:44 -04:00AEPt5sAEPt5sAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt;ASPt;B1Pt:B1Pt:BCTSBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?PTDATA ?PDATA ?PS2DATA ?P҂DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~~\LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMUi_P1RLUdP1WDUn P2AMUw]P2BAUU|P2RLUrPBASUUTbPBASUUR PCONUUb`PCONUU` PDMFTrPDMFQPLOCU[aPLOCUVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT $RUNT  RUNT / RUNT  Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPL!!TQSPACMSPACQSPACMSVER4.0SVER SVER,,/Scal@Scan@TUBEB3Tmpr<UserphARphCHRphDYbigphQLcphTRphTR,This is the confidence range *ABI_Limits..FVocAEPt5sAEPt5sAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt;ASPt;B1Pt:B1Pt:BCTSBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?PTDATA ?PDATA ?PS2DATA ?P҂DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabAFVoc0FWO_GATCFeat00GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~~\LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMUi_P1RLUdP1WDUn P2AMUw]P2BAUU|P2RLUrPBASUUTbPBASUUR PCONUUb`PCONUU` PDMFTrPDMFQPLOCU[aPLOCUVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT $RUNT  RUNT / RUNT  Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPL!!TQSPACMSPACQSPACMSVER4.0SVER SVER,,/Scal@Scan@TUBEB3Tmpr<UserphARphCHRphDYbigphQLcphTRphTRU[lWQXb^kOptpuD:\E+mHY`/&mayX9'-vyNJRA=57AMjCW`D/z\|DX&_lp2]m"]P"F77sI_6sKGol83p{ ]GxNu~)B*HUrG\W\lYT`M=4T*R7R8~f)NK[>Id`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{cokB}qHqHStXK#XTE-=[c}Sn2M5FiK&=RSbXW,62DmOYX/D?**s$[-p+D42F7f6QuA!9@1%ilJG82G [7P^E$62:gt]puL~$(ZYo|ESRDujXTE-yh;}S$oa$N;172e,qz7|!y]18`'|_n?26.?bFWJQ:Ocl2[ALYNBOTTeq:AGRD6iOGaFlPBK/axJK[YlyjSu3]aC^DfF<7:cmj^sSU|MkO_b2(_CL,vnQ6?ZdwYc_`LJ6txWTikt72WevdAVI+6[,9a)h SJXVHr%K-fU'C,_,qz7h}l6t8OGz^n7aC.?bFW?E)X~6"den?,}!KPk;+s4U5hf6]X?K90lV5FaGwpBz.\_'glxU)Bk+7{lZp4[8e7?GOVcRohj4TIx*&`f5U_9%jE6;