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] ] ] S S h v v """...999DDCNNNYXXbbhkktuu}}} < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < ; ; < ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5275b3b9f4c847f0b16669581c9cc595Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-06-04_18-54_0817 3130Seq 12554 12554_Steier_K1340_matk_R jmadera 96-Well
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v5 E c 0 . 5# &+ 'g '=vAGTAATTTTCGAGAATTTATAAAAAAAGGGGAGATATACAATGTTATGGTGAGGGTTAGCTGTATATGCGCCGACGGCGCTCACTATTATAAGAGTCTGATATGGCGCCAAACCTCGCATAACAACGAGAAGGCGGGAGTGCGTAACACTTTAACGATACAGCGACTGATCAACCGGGAGACATAGTGGCACCACTACTATCACCCTCATATGTAGCAGAATGGATAAGGGGTGTGTACCCTAGCACCCGAGGGCGTGCGAGGGAGGACTCCAGCGGCACACTGGAGCGATATCATAGAGGGTGAGGAAAGTGATAGCATATTGGCTGCATATATCTCGTCCGGGCTGGAGACAACACATCACTTTACGATACCTACAGGTAGACACTGAATGATACCTACAAATACATCACCCGATGCTTCTTTAAAGAGGAACGACTAGATACCCTTCGTTACTTATCAGAGGGAACGAGGGATTCTCACACTATCAACATGTACACTCGTTTGGAGGGGTCGCGGACAACTACACGATGTTCTATAGGGCGATAGAGCTATATACGCGCACTATGAACTCTAGAGGATATAGATAGTATGTGTGAGGATAGTTGATGCAGAGTGAAGAACGCTAGATACACATACTCACACATAGGAGTATATATCAGGAGGAGTATTCTGTGTTACCTCACGGAGGGGGAGGAGCTGTCGCACTCGGTGGGGTGGAATTATGACACTCTGTCTAATAACACTCTCAAGAGGGAGCTCATTATTTGTGCGGAAGAAGGACGCCTTAATATACTAGAGCGGAGGATGTGCAACGAGAACTACATATGACTGTATATGATGATAATTATATCTCTCCACATCACCTGTTGTGTAGGGTGTTCCATCTABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-06-04 20:24:03 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN O APrV 1 APrX } } X ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ! B # CTID CTNM CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ! B ^ DATA ! B DATA ! B DATA ! B $ DATA +' VN DATA
+' VN : DATA +' VN , DATA +' VN z DCHT DSam DySN Dye# DyeN DyeN
DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! % LNTD 2 LsrP a MCHN MODF F MODL 3730 NAVG NLNE ` NOIS = P1AM y ` P1RL y Y P1WD y g P2AM y u P2BA y y | P2RL y n PBAS y y A| PBAS y y > PCON y y VR PCON y y R PDMF p PDMF = PLOC y K PLOC y D PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ۹ RPrV 1 RUND
RUND
RUND
RUND
RUNT 6 RUNT 7 RUNT
1 RUNT Rate RunN " " S/N% = SCAN 3 SMED
SMLt SMPL e SPAC AXf SPAC = SPAC AXf SVER 4.0 SVER | SVER , , f Scal @ Scan 3 TUBE H1 Tmpr < User phAR phCH = phDY big phQL c phTR phTR %߁ This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN O APrV 1 APrX } } X ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ! B # CTID CTNM CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ! B ^ DATA ! B DATA ! B DATA ! B $ DATA +' VN DATA
+' VN : DATA +' VN , DATA +' VN z DCHT DSam DySN Dye# DyeN DyeN
DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! % LNTD 2 LsrP a MCHN MODF F MODL 3730 NAVG NLNE ` NOIS = P1AM y ` P1RL y Y P1WD y g P2AM y u P2BA y y | P2RL y n PBAS y y A| PBAS y y > PCON y y VR PCON y y R PDMF p PDMF = PLOC y K PLOC y D PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ۹ RPrV 1 RUND
RUND
RUND
RUND
RUNT 6 RUNT 7 RUNT
1 RUNT Rate RunN " " S/N% = SCAN 3 SMED
SMLt SMPL e SPAC AXf SPAC = SPAC AXf SVER 4.0 SVER | SVER , , f Scal @ Scan 3 TUBE H1 Tmpr < User phAR phCH = phDY big phQL c phTR phTR TrU]wfg6v;(vJ4;iD4ZI4$%\e]yaQ>2nxHBmHle8hmYh^n_k@0{RRV3?wrmJGLbva].p>x'[IoOoq4^M1}GVRI<672_4CF_E[UO')JRr+4#Mc]_^ave>uDs5iqbyMimE%ToN6|1:#<'r{?:TA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]oT2mt1A'obU2tRHef&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`#?Q{6Jn`BT]{?v2FKeS5~i2oru-<7X_ Wl*ugmp]sUOzjge`HB! P8X-o_}4]'G' YHT+(aQQxnJilXSXW .C=vz^`%C]Cp3cVumgHr94zqm}s zyH+r,]_03BSmv^N6T.iP2i#G2rfJX|7"Wa%#;-T)RJ0vrs|%3b0FcS==6X12g[jWcgEQm*Y6i47.z.\_'[wA#Q9U,':ZKr]#Ko5qSF1_T6RA0W7yLl-j(B~W^17a.h^n@EK5llmd$XF$}IGq8->~+gx/E[fD1E?K90c_ax]]?EZp9}:>Jdq3+fFkT/n@6TdO{7>kOptpuzpEey!!fEAx8aPI^QJH){!9Y2]2rRJm@=%FEh_@dUa0u:W^$bePX-N&Wv'i2{M~yHAj}="WlKubQ2a5rjzU4o[H;E12v-T)k\Dr^5} 1{T/(J8dM!tX^"qrB%P^gW?ivd9f TA,h{|dsQ;T`h3/[zS2Mmn[/EZdwYc_`k=`<{!,8#V>Axd$XF$X?;9y8hI0.wv|$@^]?\Hif3qE`HoG83:8\_pf|r,~ &~]u7Q9uL9"c.VDTj.uFr~o[|!Y<,h`%mI{Mb=KubQ2a5qO Jm}Pz;+hj3ST?0sKG'BNp3<("Hf.Q F3eG"k@j/.3]J&sCe#&\7`[qDr_WG\|J_j.u%xfmFoM/V,<{1z^{5te.ER]QZEpBoz?f.T|*1fCy]_^avep"iK]jSBrRL8~f)NKU|t2#71B!Hiwe2{sGitGK:Q&:C!g61CfF{\)lOY }YWoa$1pfnST/4f!Z56N|&ZG90Si! AEPt 3 AEPt 3 APFN APXV 2 APrN O APrV 1 APrX } } X ARTN ASPF ASPt ASPt AUDT d d B1Pt B1Pt BCTS BufT ! B # CTID CTNM CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ! B ^ DATA ! B DATA ! B DATA ! B $ DATA +' VN DATA
+' VN : DATA +' VN , DATA +' VN z DCHT DSam DySN Dye# DyeN DyeN
DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! % LNTD 2 LsrP a MCHN MODF F MODL 3730 NAVG NLNE ` NOIS = P1AM y ` P1RL y Y P1WD y g P2AM y u P2BA y y | P2RL y n PBAS y y A| PBAS y y > PCON y y VR PCON y y R PDMF p PDMF = PLOC y K PLOC y D PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ۹ RPrV 1 RUND
RUND
RUND
RUND
RUNT 6 RUNT 7 RUNT
1 RUNT Rate RunN " " S/N% = SCAN 3 SMED
SMLt SMPL e SPAC AXf SPAC = SPAC AXf SVER 4.0 SVER | SVER , , f Scal @ Scan 3 TUBE H1 Tmpr < User phAR phCH = phDY big phQL c phTR phTR