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" "            $  $' &         !                      !&               #   !     #           # $*""!*$-( '"#    ! )#* )""       !    $")           !$ $#&" &!,! !"   *         '& (   %        ! $  %       (                   $                 !              %           %() %")          & #                   %)   !       ##               "              HIJKLMNOPQRSTUVWXmnopqrstuvHIJKLMNOPQRSTUVWXmnopqrstuvE,,,III^^^{{{&&&99DDDNXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR2079;;;;:99875320/-+('$#  ]]]SShvv   """...999DDCNNNYXXbbhkktuu}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ef5311ff79144c6899862ee0c257ed77Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-06-04_18-54_08173130Seq1255412554_Steier_K1340_matk_Rjmadera96-Well K1340_H11_RKB_3730_POP7_BDTv3.mobSeqPop7zsyC7DHRhk"jJ|9\*VRLf2CB?AKSI"rO;b%n: sU`QaXXCy4fb3'tt*1t6kx_5 a=&gpDU4PcvyIkFJu!"pL[   2 . 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(IagZ= 'FattS3#*HqS6(&&#  +78/ $&   "+/-& $-.(*33+!3FSVM9! ):DEA<87884+   $-.&+GXXH/ )580  &/.$$5<7( DxvM'#A^nlZ?# -=?3 $8B;' ,32,$:\sxiN1       !9LSL8'0."/;:-  '02-# ).(!XzG<mW)  "% ,RqzlP::UV?>JWZP:!  $,*!  6LSI1.J\`XI=7;CKNH:(,Hbv|s\= &28=HZswS,  >Yjpnlqvpx}kU<" %5;4$!)'  '( ,DPJ7"$>[qwm^RRZbbS8 **!  #-0* #''#  " 5KRC( )LceP0   Oq> +9DHB4 !1<@9( )Jdk_C$ &:DA2    @`rp[<"4??1 $>UdhdZK9% '18<AGNSTPH?70*").+  "=Yny{ywzveUNRbujF ;wd,ItmD &KuU0!8Zvx[5"*(  ?^lcDBlb9&*# *4;DMVZUC) IusO3*6TwfOA<<<;<BLYbaU?&(00)@eu[C3(! )?NQD.$..%  $Ij|x_9!9EA/','  )0,    !A]jaE" 6B=,   %! ;TcbR8  /5/!   ,GXXF( &34(  0>C>1!7]`: 4EI?+9[u{lL( %'&&)1>JQOC1 5GPJ6!Grzlmz~U,    %BX^R8  DcocB0M[WG5-2AQXR@(%,-' );M[b_R>( 3LVM3.BF8!  7K\m{L32E]kgQ1#7IXcjmkdVC- 2TlrbC! $YV/ '6>5'OozlK#:\tylP0  (6?A;0#  *Ime=  #%" ).)  .=C<- @;A(AFA>BKB.bcpKB_3730_POP7_BDTv3.mobtermCCAGGGTCGGTTGCACCCACGAGGGCCTGTGAGAGAGAGATTTCGCCCCCACCATTGGTTCACCTAAACAAAACCGAAAACGACCCCGCCCGCTTTCAAGGGATGCCAAGGGGACCAATTTTCTTTTACAAAATCCTGTTGGGAAAGTTAGAACTAGGTCCGCCCCTCCTATATTATCGAAAACTTGCGATTTACCTTTTGACCCGTAACGGCTAGTACGCTTGATGTCTTTAAATTCAAGAGAGCCGGAAGGGCTACGAATGATAATTCGAGTTTCAGTTGCCAGGGTCGGTTGCACCCACGAGGGCCTGTGAGAGAGAGATTTCGCCCCCACCATTGGTTCACCTAAACAAAACCGAAAACGACCCCGCCCGCTTTCAAGGGATGCCAAGGGGACCAATTTTCTTTTACAAAATCCTGTTGGGAAAGTTAGAACTAGGTCCGCCCCTCCTATATTATCGAAAACTTGCGATTTACCTTTTGACCCGTAACGGCTAGTACGCTTGATGTCTTTAAATTCAAGAGAGCCGGAAGGGCTACGAATGATAATTCGAGTTTCAGTTG #2CTgw#-@Tdw )8DQ\j.AR_q*5ALU_mx)3IS^jw&6FL\m|&2?K[hy*4?P[ft  * 5 > I Z i y    ( 0 ; C Q d p y   - 9 C L \ c m y   0 < E T g w   * 9 J U h p | %6CTany #2CTgw#-@Tdw )8DQ\j.AR_q*5ALU_mx)3IS^jw&6FL\m|&2?K[hy*4?P[ft  * 5 > I Z i y    ( 0 ; C Q d p y   - 9 C L \ c m y   0 < E T g w   * 9 J U h p | %6CTany                                                                                                                                      6*C>M7,%>^ECYI($"B*#&JK;6 _-%^V9Q+1d7$HTb? +IM@D3$!$;*> 9GYP'6@ *CQV9OXY@0<L*Y<)PPBII)WbQ ;H2R'adJPJ!X, 1E''5b4-baMX/@_04SCOUZZA/U 6?_@K]9AT]5b/:RUD-_Y+ \/Y+,W2:OWV)']5 3&5Ic V8]E.M-\*%D? .#8GH/I$A9)`JL5 1OS*:k}3c!  7U KJeGs WC4f:e#@mD$8Y8V{.M{ ~tG^ [7M3M!-<'nK}a`!P$t [Q$JosB{0>oO$mv" 5n4 :P5R r)U}wQ!*~Ibw4; 3-i^u-[!8zx@B_07Y BK6ptnXERr4_%S5!h*-$#SI%/kk%2 Vz]\RK +!R/x@ C &GA+5Pr  = " ~_gF69tNx57 d5nO+,tq;6.Ips=(mxy8,v 6>PwIGE, , $ ` |{,vS01"dcQ.,!&}, Q=db'H,N1@t-t`ndtS2r,$&| uX{1m.>8dcx $ l TOi:2E9 @Ub:7ZYm-<>4@v: GR!)"-H4]F(O'Oqp?C0cx? nRC8O'+X=]( $3/oL6 b~>fq@R0$dl+Nf'QH=33P$wd 2H!`f ^M 0c6:0D nHwJP1LAAGAATATAAGGAGTAATTTAGCCCTAGTATTTGTGTTCTGCCTTGAATGCGACACCAGCGCTAATTCACGGGTTTGGGTTCGGTGGCAAGAGAAAAGTCCAGGCTTCTCACTTTAGCAACCGCACGGACGGGCAAACAAACACGTAGGGACTACCTTCTATATTTGAAACGTAACCAATTGTAAATATTCCCCGTACCCTGGTGTGGGGACGGGAACGCACCACCCGACGGCGCAAGCCCCAGCTGACGGCTAGGCGATTAAGGTTCAACTCCAAGGTCCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-06-04 20:25:27 -04:00AEPt3AEPt3APFN `APXV2APrN 'APrV1APrX}}0ARTNASPFASPt{ASPt{B1PtzB1PtzBCTSCBufT!BCTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B6DATA!BxDATA!BDATA!BDATA #G iDATA #G ,sDATA #G s}DATA #G DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT jEVNT vEVNT‚EVNT•FWO_GATCGTyp!!´HCFGCEHCFGHCFGHCFG==InScInVtLANEQLAST~~LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISOfSclP1AM4 P1RL4 P1WD4P2AM4lP2BAP2RL48PBASPBASPCON PCONhPDMFIPDMFPLOC44PLOC4PSZE`PTYPPXLBRGNmRMXV1RMdN ԭRMdV1RMdXԶRPrNܑRPrV1RUND RUND RUND RUND RUNT 6RUNT 7RUNT  1RUNT Rate ¨RunN""ܡS/N%SCAN3SMED SMLtSMPL =SPACMSPACSPACMSVER4.0SVER 8SVER,,>Satdl^Scal@Scan3TUBEH11Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN `APXV2APrN 'APrV1APrX}}0ARTNASPFASPt{ASPt{B1PtzB1PtzBCTSCBufT!BCTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B6DATA!BxDATA!BDATA!BDATA #G iDATA #G ,sDATA #G s}DATA #G DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT jEVNT vEVNT‚EVNT•FTab&FVoc&FWO_GATCFeat..&^GTyp!!´HCFGCEHCFGHCFGHCFG==InScInVtLANEQLAST~~LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISOfSclP1AM4 P1RL4 P1WD4P2AM4lP2BAP2RL48PBASPBASPCON PCONhPDMFIPDMFPLOC44PLOC4PSZE`PTYPPXLBRGNmRMXV1RMdN ԭRMdV1RMdXԶRPrNܑRPrV1RUND RUND RUND RUND RUNT 6RUNT 7RUNT  1RUNT Rate ¨RunN""ܡS/N%SCAN3SMED SMLtSMPL =SPACMSPACSPACMSVER4.0SVER 8SVER,,>Satdl^Scal@Scan3TUBEH11Tmpr<UserphARphCHphDYbigphQLcphTRphTR]nd3ghlRc T-* +Wt3z{0bInTor@tbfD7E)0Ie/AK8N'nsv&'Y?sxWlZN.YRz!#fn2%FT!sz^2h3)-I5d~)Uk}`:r&xvr_WG\|8 Xo2h{/S9~i,EAx8amb#QJU0iHAj}W2yv!a!<}B]rQHfKbVU}Uz1p=|(LZjp7xMO9j;q6TB(2JLvuUlN1@#vQ!TMF4FprjDp*K0.]+0Oj!Q4aK{WlVO:}mldKzM ZmnHN{$By%mat5+y|`}__';_/A@(#jFnjUZ'KvNIGQ5ENI@[wQi^*w% enqb[*jG3T%ycX}%z/q1wv;{?kJ:hAqTFU-vbNhsrU5-uvCbbJ_<$AJu"#p{J*aWUB@6YgroH6iAC?-4$&)-%TwK[*oCfty5lYMI){mnjH[$.}RZ#3|>oW~!zFHyErN3W4KD@|Q$OEH< z!$w1QjXaM~PG"BXP2gXBrRLohi\`hlZZdBc'}_~:H^)!,8Kp/(ZA$den?a+b8E|W,K4vqb>H\R~.i58f{sGi8AS_9\qwH=qd)B*vH%lFUT;fQGE-`x&iG_C26R&^8o5`h]RIUwch[}k@_a'>yj6m}Pz%W%43q*)_RhGlB^PY6C}B-m2yy>T\3>>B]'G,lwdRadJsV&=o?eU&GUkJZm{Z(c&H+b+M_:L:Z+D$9i1>Qu3NM|5O-U[ds=:I12v-T)D@0vrs|%3lC(`J8daxF![pE)}Of'PJA}*4d#aY&1FDHYWm;2FVcxS3"6%4y^'a,k){hI{?7.N{C]QHW>ycPX*F8Ba+b8E&N6|MR_le/M#yKAI8rBAr_s_bed{a9_ajF=W.X}=_;!;`sEYWC.%ouO{9X6!7&o.jaxJK[Yl)d762H#A#Q9U,7Yv^TTH455-z]G3o6RlSR#yK9YtIRPV]O_,A8 z9X=5>2jP@*F8a?G>Sx+C!K}Z'BNpbX\"B=8djF3u2yl:[>zw#NRd)B*OqPk&OE6OLxPo%?E7\lzqdY(xs/b@j/.3]Hq<6(m=':Z^u4[42g|5PxKoJ-[CW?R5Ch2d'jw~|>J3zi^{g5AEPt3AEPt3APFN `APXV2APrN 'APrV1APrX}}0ARTNASPFASPt{ASPt{AUDTff6pB1PtzB1PtzBCTSCBufT!BCTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B6DATA!BxDATA!BDATA!BDATA #G iDATA #G ,sDATA #G s}DATA #G DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT jEVNT vEVNT‚EVNT•FTab&FVoc&FWO_GATCFeat..&^GTyp!!´HCFGCEHCFGHCFGHCFG==InScInVtLANEQLAST~~LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISOfSclP1AM4 P1RL4 P1WD4P2AM4lP2BAP2RL48PBASPBASPCON PCONhPDMFIPDMFPLOC44PLOC4PSZE`PTYPPXLBRGNmRMXV1RMdN ԭRMdV1RMdXԶRPrNܑRPrV1RUND RUND RUND RUND RUNT 6RUNT 7RUNT  1RUNT Rate ¨RunN""ܡS/N%SCAN3SMED SMLtSMPL =SPACMSPACSPACMSVER4.0SVER 8SVER,,>Satdl^Scal@Scan3TUBEH11Tmpr<UserphARphCHphDYbigphQLcphTRphTR