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] ] ] S S h v v """...999DDCNNNYXXbbhkktuu}}} < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < ; ; < ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8c4c8a722baa4f8eb7cc0f39e5e12249Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-06-04_18-54_0817 3130Seq 12554 12554_Steier_K1340_matk_R jmadera 96-Well
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% 9 GTTATTTACCACCCCCCCCGGGAGATATACAATATTATTGAGAGGGATACCTGTGTATACGCCGACCGAGCGCACTATTATAACAGGATGAGAGTTAGCCGGACCTATCGTCACAACTAGAAGGCGGGACGGCGCGACACCGTAACGAGAAAGCGACGGAGCTACCAACAGAAGTATTGGTAGCACTACTCTATCCCTCCTCATTAGAGGAATAGAGAATGGGGGTGGGGCCTCGAGCAAGAGAGCGCGGGGCGGAGGAAGGAAGCGTCGCACTCGTGGAAGACCTTTCCCGGAGTTGAAAGGGAGGGAAGAGGAGTTGAAGATATGCCTTCCTGGCCGGAGAGAACAGCCAGCCGTACGATACCTACAGGTAAATACGGAATTATACCTTTAAATACTCCACGGGAGGGGCCTTTCCAAAGGAGGGAGTAATTAAACTTCGAGACTTCAAACTATTGAGGGAGGGAGTATCCGTGCACCATCATTTACACTCGTGGGGCTTGGTAGTGGACACATCAACGATATGCTATAGACCTTCAGAGGTATATACGTGCTCTATGAATGCTACAGGATATGGTTAGCATGAGTGAGGATAGTTGACACAGAGGGAGAAGGAGTGATACTCATACTCATATACATGAGTATATAGAAGGAGGAGTAGTGTGTGTTACCTCAAAGAGTGGGAGGAGCAGCGTCGCTCAGTGTAAGAGGAATTATGACAATCTTTCTAGGAAGACTCACGTAGAGGGAGGATCATTATGTGTGCGGAGAAGACGCCTCCGTTCTTAGAATAGCGGAGATTGAGCAACGATACCTACATATGATGTAAGGTGTAAGAGTATATCGACATATTCCTTTGTGAGGGGAGGTCTTCTCGCCCCGAGGGTATTGATGAGGTGAATCTGAAGTAATAAGGATCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-06-04 20:24:51 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN O APrV 1 APrX } } X ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS t BufT ! B # CTID CTNM CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ! B ^ DATA ! B DATA ! B DATA ! B $ DATA , Y DATA
, Y >F DATA , Y DATA , Y DCHT DSam DySN Dye# DyeN DyeN
DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE / LAST ~ ~ LIMS ! ! % LNTD 2 LsrP a MCHN MODF F MODL 3730 NAVG NLNE ` NOIS Kp P1AM 0 o P1RL 0 hc P1WD 0 v P2AM 0 # P2BA S P2RL 0 } PBAS O; PBAS K PCON d PCON a3 PDMF p PDMF K PLOC 0 Z PLOC 0 R PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ۹ RPrV 1 RUND
RUND
RUND
RUND
RUNT 6 RUNT 7 RUNT
1 RUNT Rate RunN " " S/N% Kh SCAN 3 SMED
SMLt SMPL e SPAC AQ SPAC K SPAC AQ SVER 4.0 SVER i SVER , , f Scal @ Scan 3 TUBE A5 Tmpr < User phAR phCH K phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN O APrV 1 APrX } } X ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS t BufT ! B # CTID CTNM CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ! B ^ DATA ! B DATA ! B DATA ! B $ DATA , Y DATA
, Y >F DATA , Y DATA , Y DCHT DSam DySN Dye# DyeN DyeN
DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE / LAST ~ ~ LIMS ! ! % LNTD 2 LsrP a MCHN MODF F MODL 3730 NAVG NLNE ` NOIS Kp P1AM 0 o P1RL 0 hc P1WD 0 v P2AM 0 # P2BA S P2RL 0 } PBAS O; PBAS K PCON d PCON a3 PDMF p PDMF K PLOC 0 Z PLOC 0 R PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ۹ RPrV 1 RUND
RUND
RUND
RUND
RUNT 6 RUNT 7 RUNT
1 RUNT Rate RunN " " S/N% Kh SCAN 3 SMED
SMLt SMPL e SPAC AQ SPAC K SPAC AQ SVER 4.0 SVER i SVER , , f Scal @ Scan 3 TUBE A5 Tmpr < User phAR phCH K phDY big phQL c phTR phTR -Dq.$+W x:H-p9bgh`U*lO6viO$@I{-!`:ZPV2fI&$yNihB:Drj{H,:MX]#=c$DfqcdsTF]luKgk]35FQ6nB!X`7.w>6?xHf.QR9JK[YlyjSu30&reA .3zqg94$4$A8V/b|m:hN{+eqL}4|Md'ac_`k(U.*C]J=E]1|eKW3CNIG2gbXW,VE+0&'7%4X+*fl979v$sp3@=?]p&q!R;{?kJ:h+p?H(+Q;T`h3/[ze/G,y"o-,(B~b) e:=8}R"wKgpjm2D%~4r8h98&>LbsMvF(wD1RuM]\K+d{o&!i_6,? W]H6X+9mvH%lFU?-4$&FkNp&iJ-[qr[&Xj|82Z~9}j~:HzAb9ECQ/}Z&['K2;+hj3ST?0sKG'BNp3JWTX+9_e VO[O~}4}XfSap<#2_<2R57}?hik?]wJUo6X?/WZ#&a:Oc!X|eDNJ{s3x>rb TQ3U'.QR0Iel1}ssb[l"G>=?]p&qqQIxw}H{-*zir8rP p'Ic62nFT16Y=*"KzM ZmnHm{OYy!m]t5+y|`}__';_/A@(#jFnjUZ'KvNIGQ5ENI@[wQi^*w% enqb[*jG3T%ycX}%z/q1wv;{?kJ:hAqTFU-+%3N;KBY[n8&AzZG&nak'eaK|`h3/[zeqr[&Xj|82Z~9}__';_/e"^=0`VZI;P>W+YYo%+0|5Q1V?ORJedjY{mwYBTU;CxhKn6[MHSVi220b_'{aCA{%?_CjFDhECel7B]%jxxsWDy4<|O{^Mm[*&`_@-|T+a6oGhnWe^kQtA+UZ~en?,}&Dg8YauBQBhwD~EG7&o.jax]CR"lWW .C&qN^Fu*tw9E^u4[Jn\9-s1tog,s48tNhA&!qH$ AEPt 3 AEPt 3 APFN APXV 2 APrN O APrV 1 APrX } } X ARTN ASPF ASPt ASPt AUDT d d B1Pt B1Pt BCTS t BufT ! B # CTID CTNM CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ! B ^ DATA ! B DATA ! B DATA ! B $ DATA , Y DATA
, Y >F DATA , Y DATA , Y DCHT DSam DySN Dye# DyeN DyeN
DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE / LAST ~ ~ LIMS ! ! % LNTD 2 LsrP a MCHN MODF F MODL 3730 NAVG NLNE ` NOIS Kp P1AM 0 o P1RL 0 hc P1WD 0 v P2AM 0 # P2BA S P2RL 0 } PBAS O; PBAS K PCON d PCON a3 PDMF p PDMF K PLOC 0 Z PLOC 0 R PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ۹ RPrV 1 RUND
RUND
RUND
RUND
RUNT 6 RUNT 7 RUNT
1 RUNT Rate RunN " " S/N% Kh SCAN 3 SMED
SMLt SMPL e SPAC AQ SPAC K SPAC AQ SVER 4.0 SVER i SVER , , f Scal @ Scan 3 TUBE A5 Tmpr < User phAR phCH K phDY big phQL c phTR phTR