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%/&)- (*: & #,#)16"/A$/9)&44:/<(+9:!&9&9+'-=2#(6$3'.&-4'#,0)/%!'')1'+#"15'1&7*.070497"3((6)1,36&2@+)!+2%+3I);4,*0+*))8.)1(=$1.&"*.;)"6)*&08-48%&.#23456789:;<=>?@ABCDEFGHVWXYZ[\]23456789:;<=>?@ABCDEFGHVWXYZ[\]2Y,,,III^^^{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR /:>??????>=;;986430.+*'%#   ]]3SSSwww  ###///:::EEEPPOZZZcccmmmwvv~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4abc11e4e4224e6aa35a467e600995ffSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-06-04_20-24_08183130Seq1255312553_Steier_K1340_matK_Fjmadera96-WellK1340_E9KB_3730_POP7_BDTv3.mobSeqPop7z        #%)-16<AFKPV]fp|*D\teH+ "7Om$Z &33"^!]<%";h\~LGl   : M l!FW &O[ GXR3khE- rdWME?=<>@ABBA@ACGOZhxlXF8.)*3GfCEdF4*&(+058:974/*%%Cx4Lq/H)TuI# (2;CINPQQNKE@:4.)$                 ")0:DRcw6n1t:xFf}yaB}wsnic^]aky#g[)}g^4 8 ;  s m  V   R  y|m~uc^( q  P7 i8gM8( #3R5kyJxP0}qg`[YYYZZYWSNHB=978<CMYhy$-36752.++2Ddd!=?%H35UsA^75EHrmW8,*,..-+(&%$#"#$&)-035552/+&!                   '.8BO`v Du6_ RwH}bH1 $(+1>Vv|K %.+ /{gx,[`6`2;{cF8\Z |n{)m9 X2nIarUB8557:;93*  3Sn7Q`aT<tV7CMQ!7C=#m"b8:}z/s(X4m>& #&()(&#     "%&''&%$$###""!              %.9FUfx)@[x jL0.Px'%,ed?~S%)0.$tC$!1BPY^dkuvaN?5/-./012247=GWm&22&pT?/#%2F_}lP8&  "%')*(#      "$&()*+,,,,,++*(&$"     m@ACALTbn$-BQ]fo &5;AHSdq,7CR[eu %09ES`ju} -:DTcr})2>KXfu    ) 3 ? I S ^ h t   , 8 B M X b n y  " 1 = J Y b l y   ! * 4 @ L T a j s ~   * 8 E O X e r +:GU^ht} $.4;BIU^k|'4@MZgr{ #,>LTbn$-BQ]fo &5;AHSdq,7CR[eu %09ES`ju} -:DTcr})2>KXfu    ) 3 ? I S ^ h t   , 8 B M X b n y  " 1 = J Y b l y   ! * 4 @ L T a j s ~   * 8 E O X e r +:GU^ht}                          "                                                                 "                                       &\PX@@aH]F%F /9;adPa]Y`CU)/ R<KZ[$:ba+ab+]OY) Wb29R-0R<2$K'c<6 ;a; Gc (7, 7R9UAA c\. \W2>@/U2_  BOdaN1]%+; +6 D\ WE+N V3B\@3`)W>2D ;_1("ZF'# [V`E 2!@@ >@-Y@I?_YZ$@X@*9a!$@'KK8H22KK1#KcKO`K2K\D2\Kc22_KZKK9aW 2 b0K-8b QKLKKFK.W']R<d%=kpJ+&j&#J\"nI "PW6 i-U7u*6c8F)  5Q]lQum "3f{f tI\uDulzfdInOer5}#'!#`B F,vTn'C ;#j  1_. ,zAKinfXMGEsA-?v|,#9[DKPC=LJ~@sF/i`,t7-3\qu+ Py/3  Vn.|!WVJVST8 Gs (g*sX9jO6m$I^dW{_q7jsL=#',+6 9=#P!g^14 5}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}d8p8p8pdpQ*0b Z56<Xa,"4qP8,I1>B{}\.g C+VKp8Zgzc{^y8,1B=60P"$4yppdeF}Dd* Fn#W^d L0/p<w$/d& al.? i CTCuyKzAH&9h D )?Ds3"85IZo>4i8QsZyi* Jg/"3'4Q<ZdQeh!T  AI_$y+V/< AG2=C6Z-C7TnUhVINn,25="![b*)cagY"uWOd5V;=\A vbsJh[~ YncEj` CATTTTTTTATTTTAAATGTTTTAAAAAATATATTGATTATAAATANNCGTCCGGAGTACACTTTCTATCTTCTTCACCCCCAAGGGGGGAGGTCCTCCCCGCATAAGAGGAGGGTCCCCCAGTTAATAATCGGGGTCCCTTTGGAACTAAGAGTGGGAAGAGTCATACCTCTCTCTAACTTTTCCCTTCAATAATTATTTTATAGAGTGCCAACCCCCTCTCTAGCTGGAGATTTGAGAGGTTGTTGAGGCCCAACTCCTCCCCCCGGGTTCCCCAACGGTCCCGGGGCCATTGAGCTTGCGGAGGCGGAGCTTTCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-06-04 21:56:21 -04:00AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}ÀARTNASPFASPtYASPtYB1PtXB1PtXBCTS(BufT'NKCTIDCTNM!CTOw;CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'NVDATA'NDATA'NDATA'N@DATA #GBDATA #GB-DATA #GBtFDATA #GBDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ºEVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEGLAST~~LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISOfSc|P1AM>|qP1RL>| P1WD>|P2AM>|P2BA>>aP2RL>|iPBAS>>CPBAS>>PCON>> PCON>> yPDMFPDMFPLOC>|PLOC>|PSZE`PTYPCPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT )RUNT 7RUNT ,6RUNT 3!Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER SVER,,ŽSatd|^Scal@Scan3TUBEE9Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}ÀARTNASPFASPtYASPtYB1PtXB1PtXBCTS(BufT'NKCTIDCTNM!CTOw;CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'NVDATA'NDATA'NDATA'N@DATA #GBDATA #GB-DATA #GBtFDATA #GBDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ºEVNT EVNTEVNTFTab*FVoc*qFWO_GATCFeat..*CGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEGLAST~~LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISOfSc|P1AM>|qP1RL>| P1WD>|P2AM>|P2BA>>aP2RL>|iPBAS>>CPBAS>>PCON>> PCON>> yPDMFPDMFPLOC>|PLOC>|PSZE`PTYPCPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT )RUNT 7RUNT ,6RUNT 3!Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER SVER,,ŽSatd|^Scal@Scan3TUBEE9Tmpr<UserphARphCHphDYbigphQLcphTRphTR]nd3ghlc T-* +Wt3z{0bInTor@tbfD7E)0Ie/AK8N'nsv&'Y?sxWlZN.YRz!#fn2%FT!sz^2h3)-I5d~)Uk}`:r&xvr_WG\|8 Xo2h{/S9~i,EAx8amb#QJU0iHAj}W2yv!a!<}B]rQHfKbVU}Uz1p=|(LZjp7xMO9j;q6TB(2JLvuUlN1@#vQ!TMF4FprjDp*K0.]+0Oj!Q4aK{WlVO:}mldKzM ZfnV {$7yXCit5+y|`}__';_/A@(#jFnjUZ'KvNIGQ5ENI@[wQi^*w% enqb[*jG3T%ycX}%z/q1wv;{?kJ:hAqTFU-vbNhsrU5-uvCbbJ_<$AJu"#p{J*=lSe'gum(]k@l'Z9dp"iK]jTj.uFgq%,NKo-jE(_y"%_oU@tC]2ZE<}|Uo%Z=r*r`sEY5_}4]'b5 ]{7HFwMzR93o^b,:V AmE$ww*{we*V&'Z9iS/oYFi$t.&"L4#,u6A|D2){|_nTJL:,aXj`Q Dx6m}>7:I1~8U'W33EZ'nO$`j|"/WQ`Q`zX>ykI0o6Gzv&1NmbcnP_[O~}<"&eG1<@O(LxCmpD0,_03gPV]O8oe])%|2CWllme[OCy KJFU'.QHT/}]66{6sA bBT9zH'zeE^O{U,\)lOY%}WeoVL6e-I#(c%5h(,NG:L"BHhmCW22Q&]Q#V>Ax7cXF$~y;|>EY|3.B|ux).u7z"$-m8)FTk2XS"j+OC5wz^`Mv,ZD-_p;s_|g+V3SKo)67:I1nK-k]|sOJr4{6QuA!9@1%ilJG82G [7P^E$62:gtPzuY)$yb=%AM&Z'K7rtlqaxkabC;Z6Ob"1{7"{2A2{UZBwE$X~}:>J5*m#mfXr9I&YD8J-o XS_9zH'zeE^O{U,Z:X=<;3On&+VDyK{t61h67A"yV+XZ|qP1RL>| P1WD>|P2AM>|P2BA>>aP2RL>|iPBAS>>CPBAS>>PCON>> PCON>> yPDMFPDMFPLOC>|PLOC>|PSZE`PTYPCPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT )RUNT 7RUNT ,6RUNT 3!Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER SVER,,ŽSatd|^Scal@Scan3TUBEE9Tmpr<UserphARphCHphDYbigphQLcphTRphTR