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- ) ? 5 ' 0 / / + 5 ; ' " 1 2 5 $ # - $ ? # # ! : # ( 4 ) ( * $ 1 $ 4 ! # 3 2 $ > ) - ' # . ! 5 4 ( * 6 9 % 2 5 , + * , + & > 5 . - . 9 < $ & 9 " $ , % 3 $ $ ? 9 " + . ; 6 . . . . # 0 * . 0 ) - ! ' 4 % # 7 / 3 " " $ , - ) ' 7 - , 5 8 5 ? J 7 < , 2 * 4 6 B O H Q C J A X Y X o p q o n \ ` T W Q R / G 5 < / 1 , , ) 4 7 > / 1 - 0 + 6 $ , ; 4 E 3 M K B ; E M X E K K ; : R K 6 1 D P B ? 4 @ 2 , ? 0 > 0 3 6 ' / # O O J [ i s m n j w ~ y n \ a T U G K F K C ( 1 " , . " % & ! ( 2 + , % 0 3 M S K M T M Z y } x h } h T P S I M ? C I : G C 9 L M ? ? D B M N _ Q U c Q o \ n t k c o l v ~ m z y ] e D L L S P F C I R I X L \ e b p o t u j K K i K O V G : T \ Q W Z Y Y l e b h i x d k u y | o y s z w i i h } ~ | s y } | { ~ u h e l f g b k e e o v m y x | i s w p w l I _ p u | q g W a ] P ; D R & 7 = ; 2 ; > < > ? H I G E Q 4 F G Y J T h a h W ` a a ^ f O T m _ g h c k ^ n o x m c p a ` l n _ Z e W k Z Q \ t c Z f w ^ \ Y e l a \ ] x o l U Y R T K O E A C > @ I 0 D ? 7 N M L K ? E W Z n m y ~ l b o r k s M k q ` _ I [ 5 G < @ ? 0 - ' 6 & * - ; ! ) $ < 9 ) , . 1 0 * 1 : 2 " " & ( ; 4 8 * & 3 2 0 & + 8 / 7 3 . % ) ) H 5 , - 4 9 M : a d e o h } s w x x t s o W ^ n V M b [ Y ` Z a f i u m k x { s w c z i p [ V F N : K H ? 5 < 5 5 - 0 ( 1 7 C D 8 S L Z T Y o u v w | y } o n i P b H D M U H 9 : F ; & - 0 = ; H C % > ; P T = M ; @ K [ T h ^ ` s m ] ` m d x { | n } s i o d u w W k S L U X E U 3 F 7 6 B = > N R L ^ R c U V p o e | w } j ^ ` W P G J 9 7 B : = $ G 7 = 1 4 > ) @ , I C 1 ? 7 0 . : < C , 4 0 G " 6 D > 3 C ] G N N [ ? J L ] W f \ g g q u ` x v w } u { v m r [ b ` I W ` c a Q U [ f i d o s #!D;3:*4.9/-* u r c o j k f i f q j b k o k i z v p j k n S n N H F B / ? 9 O F > 3 C 4 C Q O _ a g \ ^ o j j k q n p P Y N I S M E J = B = > 8 K M i N _ ` Y Z s r j j h f \ Y c l c ^ S S D F ? E 8 > = 9 0 7 < 4 3 = : = A 6 4 P L K S X ] b g j | y { | ~ v y } b k c g b R b L G I F G 8 D 3 B K 3 1 > 3 P = 5 7 F J P G G ^ V _ b b h u { x m e d f a O _ j d m f d b o g k { } n k r f R h S X T B L G N F S H W X M O | d m q H \ l ` h W ~ k t { x ~ p [ g q w { z s n c g b e a ` Q P A 9 < S ; J 1 2 E A - A ; N N @ W ^ u w y s j y ` X I M C @ 8 3 < 7 4 < & 8 0 . 0 ( + - 7 , % 8 / & . ) 0 ' 5 $ - $ 1 * * $ ( , ( % 0 + + & # ( # + # & $ % " % ' " ! ! % $ + 0 " * ; . 9 + . - , 8 4 4 ? > @ C 7 C F F O O V U u s w } ~ } q | p z h ^ V A Y [ T L F D A @ > : > < + H N T K Z j X r Y [ a P > P M H M C J O H Q Q N = B H D N N T X Q U ^ j s w ~ u y ~ } t f n T f b Y O m W Y M b T M W N Q b s b o v g f z c q i o t p r m ~ x f _ c g V j r x q | v c \ f [ c p X S D C < 8 D + < 5 = I C / ; ? 6 @ A G H q b p z q ~ ~ w f ` n [ O u m _ c W Q Z i O = B F E : 6 3 9 ( @ 4 0 < R S I V P 3 e T P O G V N Q X R X a ] i \ Q ` T Z ` _ F H Q Y 9 0 J M * B 9 7 1 B / 8 = 2 8 0 G C . N K X s ^ s v { l y o v ~ x o e ] i Q W C E K T L C C 8 . * : $ 6 C : < 2 ; M C I Z ^ s j x r p z u j q r w n o ` t f o f c i m Z U C Q W F D O N Y E L Z P ; K ? A O U > Q T p u h { l p v a v q n S | q f b n ] N \ O _ S V 6 A B 8 B O I Q ^ P g j ~ | t h x t s } h t y n [ } r g z y v x g u t h z p h a c o b b b e R L L T _ ? : J = & ? : : 5 0 / * 4 $ 0 6 - 2 ? 5 E 6 B ? 3 3 R Z o f i Z k c q t m s } m ] d t k a S l [ T H M 9 = 0 0 , : 7 3 4 = 4 ' 1 ! 6 + 9 * ( + # ( + / / + $ + " ! & ( - : 0 0 4 * $ * ! ' 1 % ( / ' 5 6 " 3 . % ' 0 - * * $ . , 2 ) " * ) 0 ) # " + ' % - ) # $ % ( ( & % : ) ( 8 1 8 7 9 ) - ) > E X L I k p Z b g h j t v { | t x s c q X P S g T J M ; D E 5 " K 9 E E C Q B L X _ n i } y { z ,,,III^^^{{{ &&&999DDDXXXaaavvv ***3>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR {#'()('(&%$#!
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(((222===HGGRQQ[[Zdddnmmvvv~~} < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 92a6bdcb773448fb9755ff261fcb34a8Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-25_16-50_0792 3130Seq 12522 12522_Costion_K1337_matK_R jmadera 96-Well
K1337_H6_RKB_3730_POP7_BDTv3.mobSeqPop7z 0 3 7 = G R Z _ ` ` c i s #3AQf%Kj2i 0Oi T!9Nbv}hFdA h?
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KB 1.4.1.82018-05-25 18:20:58 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA + Wd DATA
+ Wd ; DATA + Wd c DATA + Wd DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE " LAST ~ ~ b LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS B3 P1AM ( f. P1RL ( _ P1WD ( mV P2AM ( { P2BA P2RL ( t~ PBAS E PBAS Bf PCON [r PCON W PDMF { PDMF BJ PLOC ( P PLOC ( I PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 2 RUNT RUNT RUNT 5 Rate RunN " " S/N% B+ SCAN 3 SMED
SMLt SMPL p SPAC AWD SPAC BC SPAC AWD SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE H6 Tmpr < User phAR phCH Ba phDY big phQL c phTR phTR 4 This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA + Wd DATA
+ Wd ; DATA + Wd c DATA + Wd DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab F FVoc 5 FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE " LAST ~ ~ b LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS B3 P1AM ( f. P1RL ( _ P1WD ( mV P2AM ( { P2BA P2RL ( t~ PBAS E PBAS Bf PCON [r PCON W PDMF { PDMF BJ PLOC ( P PLOC ( I PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 2 RUNT RUNT RUNT 5 Rate RunN " " S/N% B+ SCAN 3 SMED
SMLt SMPL p SPAC AWD SPAC BC SPAC AWD SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE H6 Tmpr < User phAR phCH Ba phDY big phQL c phTR phTR -Dq.$+W x:H-p9bgh`U*lO6viO$@I{-!`:ZPV2fI&$yNihB:Drj{H,:MX]#=c$DfqcdsTF]luKgk]35FQ6nB!X`7.w>6?xHf.QR9JK[YlyjSu30&reA 03OZgi8$4nA8=/9Z#:hN{+eqL}4|Md'ac_`k(U.*C]J=E]1|eKW3CNIG2gbXW,VE+0&'7%4X+*fl979v$sp3@=?]p&q!R;{?kJ:h+p?H(+Q;T`h3/[ze/G,y"o-,(B~b) e:=8}R"wKgpjm2D%~4r8h98&>LbsMvF(wD1RuM]Ex+.3o&!x_N,? W]H6X+9mvH%lFU?-4$&FkNp&iJ-[qr[&Xj|82Z~9}j~:HzAb9ECQ/}Z&['K2;+hj3ST?0sKG'BNp3JWTX+9_e VO[O~}4}XfSap<#2_<2R57}?hik?]wJUo6X?/WZ#&a:Oc!X|eDNJ{s3x>rb TQ3U'.QR0Iel1}ssb[l"G>=?]p&qqQIxw}H{-*zir8rP p'Ic62nFT16Y=*"+%3N+;B9Bn8&~ziG&nak'eaK|`h3/[zeqr[&Xj|82Z~9}__';_/e"^=0`VZI;P>W+YYo%+0|5Q1V?ORJedjY{mwYBTU;CxhKn6[MHSVi220b_'{aCA{%?_CjFDhECel7B]%jxxsWDy4<|O{^Mm[*&`_@-|T+a6oGhnWe^kQtA+UZ~en?,}&Dg8YauBQBhwD~EG7&o.jax]CR"lWW .C&qN^Fu*tw9E^u4[Jn\9-s1tog,s48tNhA&!qH$ AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt AUDT d d Y B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA + Wd DATA
+ Wd ; DATA + Wd c DATA + Wd DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab F FVoc 5 FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE " LAST ~ ~ b LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS B3 P1AM ( f. P1RL ( _ P1WD ( mV P2AM ( { P2BA P2RL ( t~ PBAS E PBAS Bf PCON [r PCON W PDMF { PDMF BJ PLOC ( P PLOC ( I PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 2 RUNT RUNT RUNT 5 Rate RunN " " S/N% B+ SCAN 3 SMED
SMLt SMPL p SPAC AWD SPAC BC SPAC AWD SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE H6 Tmpr < User phAR phCH Ba phDY big phQL c phTR phTR