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* # # ) ' * ) ( ' " - ( 2 # # ( 9 ) 2 1 * + = 9 ; ( / * < C 0 ) * . , # $ % 7 1 * " # ! % ) , # # ) . 3 - 2 * ! ' 4 2 ' 5 $ 3 - ) 1 % 0 6 * ( 8 ; / * 6 , < = G ? N = < 1 H > ; 0 @ @ 7 7 9 0 , < < & & 4 < 8 4 E : ( ( % / 5 # / 7 5 * $ , A 0 > ? = L C ? D = @ 0 > F = ; J 8 . 8 - ? 7 2 = ? G 9 @ 6 / E : F 5 7 $ / 6 5 * * @ ; < 4 6 ; % : , 2 4 @ 2 / L . 6 7 , 5 B F L A S D J W k q w v u x z l m h c k D L G E 2 G ? B . ; @ ? = ? B 6 @ D I > < E 0 9 1 5 C > : O Y B B R V B Q X V [ Y h g c t v } { | k p X U J D M < % A < C G J G U B S Z S P Q \ Z D B D 8 O 3 6 2 / < 0 5 5 > B 8 9 9 1 > P ; D D A B A G ` ; Q ` r S e T c d Z c _ U O S L _ ` F ? N \ E [ I : P c V [ h z x y u m \ L P F K L W G ? K > H @ L M E ] I N O X M [ ] ^ u z n j ` e F 9 F : G 9 1 @ 9 > 3 6 P H O E U S 0 = X F M P U J Y W [ X e P > B P ? E I Q C P T b Y v ` p r Z s r y z m } x r ` Y O E D > H : C B , : 7 2 < * 8 < - 7 ' < + 2 7 D > = C > I h W b ~ y q f Y N j ` X L \ B < ? ) ; ; 1 1 / 8 - ; 3 - , - ) & ) 6 5 * 0 * 9 ? @ : : 9 A 4 H @ M < ? I @ + 3 5 ; D ; G O N J C A T ] H U > I S I E ' / 8 - , * / . - C + 8 4 ' B 2 < 9 E ) A C ? T I I 9 : = ? P O B H I : A Q I 6 > F C / - / @ ; - J = , = ; = * G A ; O 7 R F R I G P A I O V C C F J < 8 G B 0 6 E 7 ? 8 @ 3 X Q H L H A I B K I P : ( D F Y _ o t w d q } r { t a ` ` ^ I Q : ; @ 5 @ 2 P T M H U g _ T L q n i a e H F H C 0 H 7 I J 3 A L M 4 K F [ d d j p p g l q b T I J / 5 4 6 I 9 9 9 < A Q B U _ U j W b e d ^ ] G > Y : 8 M : * 2 0 * * 9 & 1 5 / ) - " , ' ! @ ; M > < A ( 7 G @ 0 7 O 1 6 % ? F : , 9 > ? @ B 5 C @ : 3 V 4 ; - : A N 1 = = 7 6 9 6 6 + / F F ; K 4 0 & ? * B 9 ; D , G : ; % ( 8 G 5 D : = 6 6 H C 7 C > D 1 9 1 @ / G L C J 5 N A O / / 4 7 N 1 - 4 7 5 0 ; ? ? 7 < C 2 6 4 2 7 + & A 5 3 9 I 5 < ; 2 3 = 0 > 4 I ? 6 = D O S M X M Z U W S V d Q Q y l z r l s } k e h q z ^ v k j r m x X b L < J V 5 : / 3 / ' / 0 ? " 4 & ( ' / 5 H B B B D H I / 2 + * & % ) - 5 0 ' = / N " 2 0 8 G I M F E I K = ; ; ; * & % 7 5 3 ( * % & $ & " 2 * ( ' 4 6 . 9 5 @ V D P Z n X K F L Q N K , > = + - . 1 > 8 9 3 , 5 Q V O K W a j Y c R V D = > ? A / * % 0 5 ! # ' # 3 ) * % + 6 ' , $ $ ' ! ) # " % $ ! 0 + " " ! / $ ! % * # ! ( $ ( 5 # # " ) + / # % , # 0 / " 0 2 % + + + % , ) * / $ % 1 ' ) - ! " # - ! & $ 0 , ! & , + + < : , ) # + ( % 1 $ 9 + ) $ ! , - 1 # " ! $ & % 2 , > - / ( , # * ' - 1 B 2 ' 6 2 9 ; 9 6 2 * ; * 8 3 / * 1 E : : 8 > 8 > # $ ' / ) ) 6 > % 4 6 G = D * N + @ C S B R T A S J F Y D ; B N b Z b i Q U X L J E K G 5 H Q J B F M 8 ; B * > , 9 / = A < E ( 0 ) < ' C D : > 3 I A C > F R Z @ D 5 4 > * > 6 5 ; G : I = @ N D R ; 8 J I Q V d c e | c k ^ f g e Z e t e p q p f v ` \ g P N V Q f < . < 8 ; A ; / K A , < 4 : / * 6 8 ; 3 ? 9 ; < 9 P \ ` S \ s t t v ] e y ] ` u r h m y q n v z e j ` U Y X P J S 3 @ B 6 8 - 7 6 E 2 . 4 5 / . ; ? - ) < % 0 # 6 3 , ) # * 7 6 & ? , + ! = 0 : 2 . 0 + ( * - ' " $ % : 2 & 0 1 $ " . & $ / # = # / ! - . ? M A 3 O < @ X @ ; K . * , 8 3 8 + / + : 0 $ " 2 5 0 & ! + - ; " ! ; / # 0 ) 2 ' ; , % + 7 ; 2 * C ( + . & ! % 5 , % $ # " $ / 9 ' / * $ " % 5 3 3 E P L ` C J ? F B G @ * 5 ; 2 " 7 2 B > * 8 < ' * 4 2 * 2 4 4 7 5 D / C 1 * @ 5 8 ) 1 " 9 * ! & % * ! $ . - 8 ( ( 3 " 1 % # * 0 5 D 1 8 1 + 3 G F 5 B K C 7 3 H 6 I ? , 5 A < = 7 6 ! % ( 6 4 1 1 3 . / . * 1 " ( , / 4 9 + 0 9 - # % ! * 3 + ) " * $ ' 0 * ! % ( ) 1 $ / 1 5 B : C = : ) 3 1 9 ; > P @ Q V P [ X e i h g U P D K I @ 6 6 @ 5 / > / & 5 * ( + # 9 & ) 7 ; 5 6 * G > 5 9 ( 9 4 . ) - + + 7 , * 4 ) E ! ? 2 1 1 + B G ? C 0 U Q Q ^ R U < S M W D Q D A N R S ] \ S d b K ) , < 2 = * / / ! $
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8ce39ea3f9a24fdabb98fdc4bd315bdbSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-25_16-50_0792 3130Seq 12522 12522_Costion_K1337_matK_R jmadera 96-Well K1337_H12_RKB_3730_POP7_BDTv3.mobSeqPop7z
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KB 1.4.1.82018-05-25 18:21:48 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt R ASPt R B1Pt Q B1Pt Q BCTS u BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA -* ZT DATA
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-* ZT > DATA -* ZT D DATA -* ZT DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE R LAST ~ ~ t: LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS M P1AM b1 P1RL ^/ P1WD f3 P2AM n7 P2BA r9 P2RL j5 PBAS P( PBAS N' PCON \. PCON Z- PDMF | PDMF N PLOC V+ PLOC R) PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 2 RUNT RUNT RUNT 5 Rate RunN " " S/N% M SCAN 3 SMED
SMLt SMPL p SPAC M SPAC N SPAC M SVER 4.0 SVER u SVER , , n Scal @ Scan 3 TUBE H12 Tmpr < User phAR phCH N" phDY big phQL c phTR phTR qk+VxnD\jE@r9,V\Nz{rT'_T%`C/&m|0locKp'" H,.9)%"ef!>b7T4lpz?Wzi b!a72BZ|7W.`4A,/|8H15KK;x+;(8W)\lZh 8<"&eG1{?v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+r(#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`@){,@Vk1$#=4]H\#D\mvH%lFU~ZYWm;2FV]#[,e/8I[X1EDE?]Rc{_@=K{_"#kt72Wer]LD_!lEH|x]0oPGlOG!>RE.$2<2oYWA8aM|I:}OU9m)nJr4_y!iU5|8e7sINi@C,_,qz7z}yaih?]R_s_bed{a9_ajFO-U[dUDNxGq@p'Ic62nrD5pY^d,/IQ}{sGitG3(|}jGPNYQIxw}H{-*<^k8e7?GOV5ro{`qafE*&aF2`_c&a6';_szT/"a/hV6T]==6X*s$[-T_sKQm5APYXA\95Lq>l>o 8:T3?qm#vRRC(vJayr=u4ZfoQe=N^tpu&ofW)NKog~!-|r< wvr]LaiY2]kO~JcW`$~~;`.pr$,.[G57'9\47.SbXW,62DmOYX/D?**s$[-HbD:/~sN91`zKA[Lz2fJq3IxdO#rT~sB=~HFF~.|#&S)BcS[7}?ef5U_9}j~:HzZbti2]2rRU)>`V|I+!K|-o_}4]'b5 ]{7HFwMzR9vAMV^@c O*Pv#$6wC(vJKq&1hE;qT%;5]U' S!oSRIoM/V/5`agvb_@U])%,1+7XM&b$SXtmx+9Hgn<_swW^$beP`C:F__diM(]y"/:Boz?&qNR0Xwt2)lOY^k'eaK|`Bt#2ze/z,(,y]18`F7IOo_3@/C 4O$ AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt R ASPt R AUDT f f 0 B1Pt Q B1Pt Q BCTS u BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA -* ZT DATA
-* ZT > DATA -* ZT D DATA -* ZT DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE R LAST ~ ~ t: LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS M P1AM b1 P1RL ^/ P1WD f3 P2AM n7 P2BA r9 P2RL j5 PBAS P( PBAS N' PCON \. PCON Z- PDMF | PDMF N PLOC V+ PLOC R) PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 2 RUNT RUNT RUNT 5 Rate RunN " " S/N% M SCAN 3 SMED
SMLt SMPL p SPAC M SPAC N SPAC M SVER 4.0 SVER u SVER , , n Scal @ Scan 3 TUBE H12 Tmpr < User phAR phCH N" phDY big phQL c phTR phTR