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[[[RRRttt (((222===HGGRQQ[[Zdddnmmvvv~~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a5360002dd8a4b1a89700cd3961dcf89Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-25_16-50_07923130Seq1252212522_Costion_K1337_matK_Rjmadera96-Well K1337_A7_RKB_3730_POP7_BDTv3.mobSeqPop7z579=AFJLMPV^eih`VKC@CIOSRMG@==AFLPRRPMID>:668=BGIGD?;88:<==;:9:=@A?;510169;94-'$$)/4773-(').351)&/43/*'(+/10,&"!%,5;>;5-%"0?JOLB4' &/8=?><;;<==:6448?GMOMHB@CJRY\ZVRQSY^aa^[Z\aglprstvxyxvtrtxwpot-Su`zM$   (-@/qN  , h#9u8>T-)=Qcv )@P]gsg!}!x2gA8Lx{Jd*`<" /U:YmvpZ3T&Cs_!xsnkjnvyohhvN /=6s2}umgegjnppnlkkkkid_[YYYWQG<1+,4@O_uO!5'r*`C/""%%#  %4?DC>:=Kh%'sV=(    )3=GQZbffaYPJHJNRRPLHFGJNS\lNHqQ:+%%),+'" $-8CGD;0'&,8IZhsz}|wn`O<*  0Kdxt`J3   3MhcI6+*1>M]iomdUE7/,-143.&   %()&!   #%%%%(-4;@B>6*  (4?DC;-    "%&'(,28;<81)     ""   #4EQWWQH=2'  !%&&%! ,He}r]H6'%.59:71)    (-.+$  !   *6@B;-  %('"%*+'     .BRZYO?,.@NUTL@3'#%#       !&),.//-)# (1:ADEC@@DMYgszzsh[QIFEFHIJJJJHFDA>:73/,*(&#    )/0-(    #%&#   &*+)#  !%(*,04661( "'(&"         -GfaD,     !#         #&'&#"*16861*#       '-.+%%*.1345530+&#!"#$# !"                                        #'))'#!###"                $&$   %&#                     "$ )253-&#*/0-(       %*-,'     '**&   !!     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I@*A1ADA6KB.bcpKB_3730_POP7_BDTv3.mobtermCATTATTGACTGCCTCCCGTGGTTGACGTATGATTGATCGTAAATTAAGGTTGTTGTGGAGACCCCGACCGCCCTAAGTAAAATGTACGAAATGTCGTCAAAGGCGACCCCCCCAAATGATCCCTGACGTTAAGAAAATTCCTAAAGACCGGATCCCCCCGGGAATAATTGGGGTTAGAGTTTTCAATTTTCTTAATCATTTTATCATTAAGAAGTTTTTTTCCCGTTGGACCTCCACCCCCGGAAGGTTTTTCCCCCTCCATGAAAAATATCCCCCAAAAACTGGGGGAAGGGAGGGAAAATGTGTTTTATTTCTATCCTTTCCGGGCGGAGCCCCCAAATACATTATTGACTGCCTCCCGTGGTTGACGTATGATTGATCGTAAATTAAGGTTGTTGTGGAGACCCCGACCGCCCTAAGTAAAATGTACGAAATGTCGTCAAAGGCGACCCCCCCAAATGATCCCTGACGTTAAGAAAATTCCTAAAGACCGGATCCCCCCGGGAATAATTGGGGTTAGAGTTTTCAATTTTCTTAATCATTTTATCATTAAGAAGTTTTTTTCCCGTTGGACCTCCACCCCCGGAAGGTTTTTCCCCCTCCATGAAAAATATCCCCCAAAAACTGGGGGAAGGGAGGGAAAATGTGTTTTATTTCTATCCTTTCCGGGCGGAGCCCCCAAATA"2AShx"4;DJZjx%1<HPYdpz!9O[eu%/;DN[hqy,<HU`hx &0CNWft~!.=JVakz&5EQ^gr|   $ 2 = J U b n ~   ' 2 ? J Y b n y   # 2 A P Y f r   ! 1 ? I T ` m u    - 9 C O Z f p }  $0;HWbp|'6BN[epz (5@JT`kx"2AShx"4;DJZjx%1<HPYdpz!9O[eu%/;DN[hqy,<HU`hx &0CNWft~!.=JVakz&5EQ^gr|   $ 2 = J U b n ~   ' 2 ? J Y b n y   # 2 A P Y f r   ! 1 ? I T ` m u    - 9 C O Z f p }  $0;HWbp|'6BN[epz (5@JT`kx    .!#        !.                               ""         (8%                   .!#        !.                               ""         (8%               [M:KR 1U2]LRZ2W\Q6 X64SaAX1,MVN*./( dEVOD+:`K BE59S&. <1R['`BPBPX MV/. L LY[\1 VTPc?P(>F7^:3 ICSKQ7(*8&= c %/!903*_4  ., HM5?K VPaU?>]2@2 PZPJ9E 3XV89:ACII&2GI?dI IT_IFFOR^0NN+bQM,0- 742F2R^R^2FGN8 ]Z2 F4"-.!^V3a]]BEJFTKJcNA&+EAGE2MEF]G;Z<!M 6aPlRF %%X$ 2  Aetk;6:h}  ?5 ! >4xoz j7;' uW"#=TV(jMXT!SLj[c\A^3[1P#~d3 LW6H~Cb %@4}  1'2<U d3nP?mR@$"tgPm![ c^ts`S H ,yf_FmPIwz a'~I0n<+|Y?m\ H?Cs&KjV7L5&F[_n,I/YUMUbP.&mVT[A\6[k'9H)-,m/Z$;0=\W8&&"w "1@m#j:P bF6,HF-   *dpC[psqK,G,#O;O^mT:mKhXR4g*,9 8SGI{_ri1]ssPk,+r3(X8j^$>xrj!k6d,cQA4&y[ASlz}nD\8dV,I`825oOu~{lIr01XP8y 8 ~O Zq_l!Q@A8}>p;pq d;R;QGEWtx>V80 A :>(M#N"h5 "07 68 c^q] !#Nl4 *z-V2$ +,{uF'p{Qtg$t.d)*t&dywO?iw!%]^zy"KrGI. vBx'-;!HFs_f).X ~U Ei~fnC:1CRsG]Bt?)*.4';cH_g8,mhD" ( ~@93a79  1[&ATAATAATTACAAGAAAAAAAAAAACATATAATACATAGCGCTTAAGGAAAAACCCCCCTACGGAAACATTTTTATTAATCGCATACTCGTTAAATCGGCCCCCGCCGATAATATTGGAGGGGACAGTCAATTAGTCTACTTATTTATAAACCATTAATGAAAGGCTTGGTTAAAAGATTACACTTTCCCCTCATTAGCCAACTCATAAGTAGGAGAACCCCATATGGTAGGGCGGCTTAATATGGCCGCCCGTTTTTCAATCTTGGGCCCAAATTTCCCCCGGAAAAAGTAATCTAAGTTTATGTGCCAGGAAACCCTTCCCTTTTCTCACCAGTAAGCGCGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-25 18:21:28 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA 9BrDATA 9BrWDATA 9BrɣDATA 9Br<'DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~[LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AMWLP1RLWP1WDWP2AMWVP2BAWWP2RLWPBASWW=PBASWWPCONWWGPCONWWPDMF{PDMFPLOCWBPLOCWPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 2RUNT RUNT  RUNT 5Rate RunN""S/N%SCAN3SMED SMLtSMPL pSPACMSPACSPACMSVER4.0SVER SVER,,nScal@Scan3TUBEA7Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA 9BrDATA 9BrWDATA 9BrɣDATA 9Br<'DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab>FVoc-FWO_GATCFeat..GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~[LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AMWLP1RLWP1WDWP2AMWVP2BAWWP2RLWPBASWW=PBASWWPCONWWGPCONWWPDMF{PDMFPLOCWBPLOCWPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 2RUNT RUNT  RUNT 5Rate RunN""S/N%SCAN3SMED SMLtSMPL pSPACMSPACSPACMSVER4.0SVER SVER,,nScal@Scan3TUBEA7Tmpr<UserphARphCHphDYbigphQLcphTRphTRYxr$c>'/_swW^$oVv+rrbf.f7y>i@UebYWg\I5Cd*9_krYZ=kZw4n,7]Ajci(l{~Gj`X0?888%g^^4tX {1t;#G)jp~0,W(YzXrDWy?0T4`FGN9\47.6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^nPz/V3QEMa!1j"SD=;LgqK-o?7.Pv_*NsPB-N&W-dOsWpC@"i@3}cUV'zK\__KdrW*`)l@.u!n`%SzXbhe >k94uL'Jd"*+8@/S(`CW7;Kn|?I)_|_n?\h["[87PFdPxS5h#ilW\|N!Gg-' 'DvddD\4A$a{|ds:MkOVc2(_CL,v.h}{ZE!]Rc{_@w,iP;YCWeq }X'>n~~2q>-kb+N4+07> YlGcRb>@iG-@yy3@=?]p&qN<;u4#k:X=<;s_|g+;Np@YS{j4zW2`&TA]BGmb,tJLV:y;`4o_=&W.: wT_no2nJBY YlGcRb[%O8{q6.>`F7u2]H!R;{?kJ:hAC.F4/oak"T'o)6 K*q3 dr153`l5A_:k9vAMV^@=?]p&q6X+9mB\yE*<^k'eaK|`P@R(RlSR[7}?e=ES77~U?63_iP-2ZE<}|Uo%Z=r*r`sEY?bM:bFs5Iis|K/l3672tXMV^A$+vTE#!#+Xn$yYE!p;|WnT(%Fy1pLze~!Tq*nEg8Z~KI'_2B.*W"i-I7} W5WDt;x.qK|>VRJxG57'9\47.<<_FVoc-FWO_GATCFeat..GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~[LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AMWLP1RLWP1WDWP2AMWVP2BAWWP2RLWPBASWW=PBASWWPCONWWGPCONWWPDMF{PDMFPLOCWBPLOCWPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 2RUNT RUNT  RUNT 5Rate RunN""S/N%SCAN3SMED SMLtSMPL pSPACMSPACSPACMSVER4.0SVER SVER,,nScal@Scan3TUBEA7Tmpr<UserphARphCHphDYbigphQLcphTRphTR