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Z [ [ R R R t t t (((332===HHHRRQ[[[dddnmnvvv~~ < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f058839729404fe1abf6e443644559e1Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-25_13-48_0790 3130Seq 12520 12520_Costion_K1336_matK_R jmadera 96-Well K1336_H11_RKB_3730_POP7_BDTv3.mobSeqPop7z !'1?Pctt\E3(#! y o g b _ ] \ \ \ ^ a c c b ^ [ Z Z ] _ b b a ` ` a c e e b ] W R P R X ^ c d ` Y O H E I R ^ j p o g [ N F E L W d n s t s t w } | l Z J < 2 - , - . - * $ " , 4 6 3 / , - 3 > H Q V W V T S S R O I A 9 1 + ( ' & # ! " ! ! " # ! " - 5 : ; 9 7 6 7 9 > D K Q V Z [ Y U Q L J L Q [ e o t u s q q t | s b T L L T d { K ' M}}O
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, 8" uW * < AATTTTTATACCACTAACAGTGGGAAGATTGATTGTATTATTATTAGGTATACCTGAGCACACACGCGCGTATCTGACTATTATAATAGGATCAGATGGAACCGGGCGGATCCGCACTATGGAAATAAGGACCCAGGCGAGACCGGAAAGCGCGAGAGACGAATAGGGGAAGAGAAGTATGAATTGGAGGAATGTATCGAGCCTCATGAGAGGAATGGAGAAGAGGTGTGCAAACTCTAGTAAAAGGCTGCGCAACGCAGGAATGAATGGGTGGGCGAGTGGGATACCTTTCCCTAAAGGGAAAGTAAGCCTAGGGTAAAAGAATATTCCCATTCCTCGTAGAAGGCAACACAGCGGGTAAGTCAGTTACCGGGAGACACGAAATTTTAACTTCAAATAAACAAGGGGAAATATCTTTCCAGAGGAGACAGTGTTTAAACTCAGTTACATATTATATTGCGCGTGGAATGCTTCGCCAACCCATAGATGCGCGTGTGGGTACTCATCACGGATGTGCTCATGACATCCTATAAAAATATATAGGTAGATACTCCCACGAGGAGCTCTACAGGACATGGTTATTATTTATGCGGATATTTGACACATAGGGGTGAGGCGGGATACTCATACTCACACATGTGAGTAGTGTATGAGGGGGGGTATCATTCCGTTAACTCAAAGAGACGGAGAGGATCACACACTCCGTAGAATAGACACCACTTTACTTATTATACGCGGAGGAGGGACACTCATTATTTGTGCGGAGGAGCCATATCCCACATTTGTGAGAGAGGAGCTCATAACTCACGTCAAGAGAGTATATAATATCTATTACAGCTACATTTGTGTGGGGTGTGTTCTCTCCTATABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-25 15:19:59 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS } BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA * U DATA
* U :T DATA * U DATA * U DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE Q LAST ~ ~ |# LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS ; P1AM d ] P1RL d V P1WD d dg P2AM d q P2BA d d x P2RL d k/ PBAS d d ? PBAS d d ; PCON d d Ss PCON d d P PDMF | PDMF ; PLOC d IG PLOC d B PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
0! RUNT RUNT RUNT Rate RunN " " S/N% ;| SCAN 3 SMED
SMLt SMPL p SPAC AZ\V SPAC ; SPAC AZ\V SVER 4.0 SVER } SVER , , n Scal @ Scan 3 TUBE H11 Tmpr < User phAR phCH ; phDY big phQL c phTR phTR ) This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS } BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA * U DATA
* U :T DATA * U DATA * U DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE Q LAST ~ ~ |# LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS ; P1AM d ] P1RL d V P1WD d dg P2AM d q P2BA d d x P2RL d k/ PBAS d d ? PBAS d d ; PCON d d Ss PCON d d P PDMF | PDMF ; PLOC d IG PLOC d B PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
0! RUNT RUNT RUNT Rate RunN " " S/N% ;| SCAN 3 SMED
SMLt SMPL p SPAC AZ\V SPAC ; SPAC AZ\V SVER 4.0 SVER } SVER , , n Scal @ Scan 3 TUBE H11 Tmpr < User phAR phCH ; phDY big phQL c phTR phTR eZ>\&bD=8d)sGi=&MQJ1rpJ=&F)9OHUkg;V<@Qn&;|>S8pu_Zj&[[Rq&!q)~'pEA({]/AT^ogqlp,D~lk;)+S8UXabCjC:AVVu>zKVJeoiQ]yw/b@j/.$I _|!{l29O[m[>Id`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{ce+bS_?Z d~)*$Fs{`jC52#GN3KHU;HBIc+75i@TH/JBcwZOHte?tH"D3|>oW~!.eHB,}sv&'Yj}4]'xP!t p7swnqR#$|$q_6U4~bUjZ*JU0._wSiVu7y,ra$Fl@i2+`'Mvn;l|tI)h'_1Z4=1)$#i#GF=fJX|7"WY2q>-kb+N4+,[Pom6e,F<_ K*qg CLNN($t@UI2c+-@[3>>B%P^gWT^Fu*t&:_Gr9#(+gbk1^/.S3xCmqy=Y`gsB0PPOw[h@tC]Q#V>Ax`X~lTmt.qK|-o3o*oUnY!9s!&&v_QjXaM~3>>//x3L#Id4|Ca$"T'MT,Tc%ToN668d#w>6?xHf.QR9JK[YlyjSu30&reA 03OZgi,$L=A8W/^Zf:hN{+eqL}4|Md'ac_`k(U.*C]J=E]1|eKW3CNIG2gbXW,VE+0&'7%4X+*fl979v$sp3@=?]p&q!R;{?kJ:h+p?H(+Q;T`h3/[ze/G,y"o-,(B~b) e:=H)RiA9GtXWJoe`rlx%b@cTY_eM6^sO \}!&R2{bPhA,K#MBE*aWUVq&!Z=c':Z^STt4)TK%FyPD[6A|zvn[Y<,h`%mI{Mb=K{_"#kI>b0FcS==6X12g[jWcgEQm*Y6i47.z.\_'[wA#Q9U,':ZKr]#Ko5qSF1_T6RA0W7yLl-j(B~W^17a.h^n@EK5llmd$XF$}IGq8->~+gx/E[fD1E?K90c_ax]]?EZp9}:>Jdq3+fFkT/n@6TdO{7>kOptpuzpEey!!fEAx8aPI^QJH){!9Y2]2rRJm@=%FEh_@dUa0u:W^$bePX-N&Wv'i2{M~6>udrV0HJA@vd*W]U_I[Ddg/