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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRA$&&&&&%$#"   [[[RRRttt%('122===GGGRQQ[[Zdddmmmvvv~~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 55803a90214645e7b273b40430c0364fSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-25_12-17_07893130Seq1251912519_Costion_K1336_matk_Fjmadera96-Well K1336_G11KB_3730_POP7_BDTv3.mobSeqPop7z%%%%%&()***+,.135531.,+++,,+*(&$#""#%(**)&#! 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E 7 [( J'.gO_D!,}V!,@Q 1/ ,?""4.7;e15=7?#.(()8A".  )  !1*E+:.1#$,!  &$ "  !           5"0"  "  "/**)%- (& 3$!+0+1</+:)$+#&&%("+2-5Jh)5l0%#.B#"BJwwH 0R]/:4F=?1-:#(T0'Ho_M& J^53jL$c!)_0isFO)AmJ6E=pWJ?8<3bi.TBCtyIZ_ncCN{y5OPUulb +!' mMKkUb]l"L.P@ )Z!xu#*A"R$% vS<)8Je|1+2 !M@D`;8~yH,:#2%6v[f)7a]F>4.F%KNf/ W&xjp /60 I\}T:;F/#F4nG#l@hsYz+:AZE^SCTTCCCTCCCGGGGTAGAAATAATAATACACATGTAATATGTAACCTATACAACCACAGGACCATACCTACAGACCCCTATAAAAGGACCCCTTCCACGGAGGAAGAAAGCAGGACCTTTTCAAAAACAACTCGAGCGAATCCCTCCACACGGAGAACAGGGGAAGGGGAGCCCTCTCAACACTACCGAGTTATCTCCCGATGGACCCTCCGGAGGTTATTTGAGACTACATCCGCTTCCACACGAATCAGAAACATCCGGAAGCGTAAACATAGGTGCCCCTCCTCAGGGACCATCCTAATAAATAAGAATGACATCAGGTAGACAAAATAAAGAGAGGAGTTAAATATAAATTGGTATAGCACATCCACACAAGCGACATCATATAAATAGAGGAGTAAGGCGATAATAAACACGAAGCATTATGTAGCTCCACGTTTATTCCTACGTACAATATACTAGATCACGAAGGGGAGCTAAAGATTCCTCGGGAAACACACATCCTCATAAGGAGGTGTAGGTATATTACCGTCTCTACATATCGCTATAATACACACTCGGGTTGTAACTCTCACAGAATATGCATATGCAACTAATAACCAATACTCACTCTAAAAGTTATGATATCACTCGTGCACAATGGGTTAATACTACATTGGGGAGTATGGTGTGATAGAGTACTCACCTCTTAGATACTGTGACACTTATGTAAGAGGCGACACACTTGATGATAAGGGCTCTAGTTATTTCGTCTATCCTCACAAAGTATAGCATCGCAACATATTCTAGTATGATGACAGTACAGATATATCTCATACTCAGATAATAGAGCGATAGGTTCGCATATTGTGGAGGTCTCGCCTCACAATCTCAGAGTTTATCCACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-25 13:49:23 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTS|BufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA *UDATA *U9DATA *UDATA *U&DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANESLAST~~{.LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS9LP1AMu\P1RLuU'P1WDubP2AMupP2BAuuwP2RLuiPBASuu<PBASuu9PCONuuQPCONuuN=PDMFPDMF9cPLOCuGSPLOCu@iPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT  /0RUNT  &1RUNT  -Rate RunN""S/N%9DSCAN3SMED SMLtSMPL SPACA[xSPAC9\SPACA[xSVER4.0SVER |SVER,,Scal@Scan3TUBEG11Tmpr<UserphARphCH9zphDYbigphQLcphTRphTRׁThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTS|BufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA *UDATA *U9DATA *UDATA *U&DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat//GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANESLAST~~{.LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS9LP1AMu\P1RLuU'P1WDubP2AMupP2BAuuwP2RLuiPBASuu<PBASuu9PCONuuQPCONuuN=PDMFPDMF9cPLOCuGSPLOCu@iPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT  /0RUNT  &1RUNT  -Rate RunN""S/N%9DSCAN3SMED SMLtSMPL SPACA[xSPAC9\SPACA[xSVER4.0SVER |SVER,,Scal@Scan3TUBEG11Tmpr<UserphARphCH9zphDYbigphQLcphTRphTR-"3oAZKC[p3}$r&kUV'4Mu*}c+-O)C@5&J06THKoS]/~8R&}[>FESt%e]:aH<6.tJdQeK~jca1n6*X!afT7+|4C9|z*yEwiO*$Fs{`jC52#GN;Fi$t.&)`|W"[XYTZ`/6?|$lQ%e+Bw9#MBz3)-I5d3g9kw6T-(WxdUo`($'tV&MWW|;WL.]_93Qq[%w%:T?NWFikaM0LBqmI,ComMOx+6T,I)h'_17a`<{!s73tt:8JX|K2MWhceS-o"KBv'o>s1HX.M;76N8{q6.>`AJP:zdq3+fyW\j&1qTFU-=DaJ2`>nX$t!TQqvLWHO$~@U)ek+M[H#S_d.u?zdq3+fFc':Z^P?ZY{7+VDTj.uFgq%,NKo-,(B~bwj{}L]^k@_ajF=W.X}=";WjcHdkabCDkm[&HcXe"t}1%j-]Tsz^`A.u?s-u@G1fCy:*?GD8Y~Csk%pcyO_lh#m|!yrA]#7w"OMbq?Eb,Z#GlOjW^]=7l1%Em[.YYo%/D^*Pis|K/l3672'[)VBT9\qwH=qd)B*E\jE@r9#$%rnLjL3ro{`N%7`h?D,kSagv`k=U@xkk^CELhhSc%bVm? >S]*|QK:}$OEH3udrVbH`6@m r;%`5vL*`~#`:c{Z.&k ~9e R0]-jE(_y"DQX;:,292CjFnjUZ:U$n?XDD[ho*9wQi:A,fv7b{Wj2oFtqwGK"j+OC5wz^`Z]k7{d&]?#5ngDVD$3#2ps'Mvn[Ca,k?]mnjH[q?Eb,u2We:lFm%FHN] 5y$47y 4orKOR`{A,}@1%i`+|*fxB^35u62rz*Rr,Z:X=<;s_|g+VFl@i2go~WR&^.9,(B~bHjAt3_iP""#]eRWJT|eiB]%j'k0WC.%ouO{9X6!7&o.ja8G$l=b d[qm#vRRC(vJnPX=<-8 )S-F3x@M9X`|W"[XYTZM%SmvlM@=K}VX*os7}Z&[Eb4;,#|>WT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS_d.u?zcw~CfF{*Tijh.iOW/D-]$/pTFT*u;SAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtAUDTbb%B1PtB1PtBCTS|BufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA *UDATA *U9DATA *UDATA *U&DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat//GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANESLAST~~{.LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS9LP1AMu\P1RLuU'P1WDubP2AMupP2BAuuwP2RLuiPBASuu<PBASuu9PCONuuQPCONuuN=PDMFPDMF9cPLOCuGSPLOCu@iPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT  /0RUNT  &1RUNT  -Rate RunN""S/N%9DSCAN3SMED SMLtSMPL SPACA[xSPAC9\SPACA[xSVER4.0SVER |SVER,,Scal@Scan3TUBEG11Tmpr<UserphARphCH9zphDYbigphQLcphTRphTR