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m 2 < ,,,III^^^{{ &&&999DDDXXXaaavvv ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRa #-1223210/.-,+)(%#!
% \ [ R R R u u u ***555@@?JJJTTT^]]gggpppyyyB < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d7166195e122477899d8a0a2ea8e24bbSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-25_04-27_0786 3130Seq 12517 12517_Costion_K1317_matK_F jmadera 96-Well K1317_E6KB_3730_POP7_BDTv3.mobSeqPop7z
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SKdtv\ ATTTCCCCCCGTGAAAGGGGGAGGGTATCAATTAATCTGGGGGTTTTTCTGCACGTTTCATACCGCACCACGGGATAAAAATTTCCAACTCCGATAGGTAAGGGGACCTCTCCTACTCGTGCTAAGGGACTCCTCTACCGAAGCCAATAAAGAGATTGTTACACTATCTCGGAGCTCCCTTCTTCTAACTGGGAGTAATGGACTCACTGTACTATAATTAGGTAGAATGGAGAGCAACAGGGACTCCTCCGGAGCGAACTATTATGGGATACTTATAGCTATTCCCGATAGAAAATTCATGCAAGGTTAGAACACCCCCGAGGAGTTTGACCTGGGGTGAGACCCAGAGGTAGCACGTTCTCATTTACAAATATTGTCTGAAGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-25 05:58:12 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS = BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA #L F DATA
#L F +1 DATA #L F q DATA #L F a DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ( LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM 4 P1RL 4 P1WD 4 P2AM 4 P2BA 4 P2RL 4 PBAS PBAS 4 PCON PCON 4 PDMF y PDMF PLOC 4 PLOC 4 PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 9 RUNT 0 RUNT 7 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER 2 SVER , , n Scal @ Scan 3 TUBE E6 Tmpr < User phAR phCH / phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS = BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA #L F DATA
#L F +1 DATA #L F q DATA #L F a DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab + FVoc + FWO_ GATC Feat . . +X GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ( LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM 4 P1RL 4 P1WD 4 P2AM 4 P2BA 4 P2RL 4 PBAS PBAS 4 PCON PCON 4 PDMF y PDMF PLOC 4 PLOC 4 PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 9 RUNT 0 RUNT 7 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER 2 SVER , , n Scal @ Scan 3 TUBE E6 Tmpr < User phAR phCH / phDY big phQL c phTR phTR ^pF?jA]RI<672_4CF_E[ .*ilw17*$xeK>tW\FU"7?-3U'.1P|qW>C^;=0hSvoIxT6eT*a@*_0t}{JTMF4F>hcDW4KuYGH[{OF%6+,/|8iHAj}W2yv!a!<}B]rQHfKb9hC?{r\*xu3{CW?vR#egxy{RB0+T.h^n@2dFllj_odF4N`j`$]O|QK:jin'Dg!~^ZUS+%+M;`8hC0rOHNJw9b6mtTUk1T:29GfnGO5bLhbB/t|4unL,rg#zv{?oH[{E,8#V>Ax^c~=$~!IZHpq'_C:+0Oj!Q4KK*flK|+@O5+ Ms~9,)jM=!%h;8w>\,$bnsYK%FyPq6!oSR{[}7&0AS_7WX773V[R"\"@-bWF'zPz\]G!3@*7_6; 0|&6X`??}@8jaA]!lZbCd]s:goT|gmktayr=u4[PngD]jTj.uFr~o[|Yya,mgrh~nRnX@EV$2kE}:>&ZgL7:tl|y\@fKr\}kYU/L+G)@-mq5FaT%ycX}OuK-fU'C,_,qz7h}l6t8OGz^n7aC.?bFW?E)X~6"den?,}!KPk;+s4U5hf6]X?K90lV5FaGwpBz.\_'glxU)Bk+7{lZp4[8e7?GOVcRohj4TIx*&`f5U_9%jE6;s1HX.M;76N8{q6.>`AJP:zdq3+fyW\j&1qTFU-/D?*{|D$haygK >xP `v?{}I8w$YWmOyl}$rv/8NpFQlUAT/n@Z-I+TwM"f^@/(-[mMOMh7&05OPh~wA[h@tFt(#jyO~6X~3"F*+#dgU[_5:W^$OufcGHO5QfOl>o ZbCd]'GE#!#hmmfc4.=+I}S$o:/bEmKB6KP8)s5AVihx_7&OMb=KuRZ+kM7yW@"SD=y!.qK|-o*u0#|UUQ1V? M;8'i2{ysPl`A(.:U q<6(mu y-WPv8U)HJxm AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt AUDT ` ` 9 B1Pt B1Pt BCTS = BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA #L F DATA
#L F +1 DATA #L F q DATA #L F a DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab + FVoc + FWO_ GATC Feat . . +X GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ( LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM 4 P1RL 4 P1WD 4 P2AM 4 P2BA 4 P2RL 4 PBAS PBAS 4 PCON PCON 4 PDMF y PDMF PLOC 4 PLOC 4 PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 9 RUNT 0 RUNT 7 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER 2 SVER , , n Scal @ Scan 3 TUBE E6 Tmpr < User phAR phCH / phDY big phQL c phTR phTR