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% \ [ R R R u u u ***555@@?JJJTTT^]]gggpppyyyB < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: b19b97bfa8ee4a769aafc6fa1efc0e5cSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-25_04-27_0786 3130Seq 12517 12517_Costion_K1317_matK_F jmadera 96-Well K1317_D12KB_3730_POP7_BDTv3.mobSeqPop7zDf| ?^~+@P^pmR3rX=#
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GAAAAAATTCCAATTATACAGCAATAGCCGCGTTGTACTGACAAGGATAAACAAAAAGTGGGCTGCCTACGACTGGGATCTCGAGCCACGTAACCACGATAAGGGGAACCAAACCCACCGTGGCGTATAACCGGCCTAGTCTTGGATCTTTATAAAGGTAGTAGCCCAGCAGCGATACAAACCTCGATCCAAGGGAAGACTGCTATTCCGCAAACAGGGGAACTCTACCGATTGATGGTCATTGCCAGTACGTGATTACGATTGGAGGCCACGCTCTGCAACGGCGAAAAGGGCACTATTTCCCGTGGCGCCATATGGACCCCGTAAGGAACCGCCAGTACTGTAAGTCTAGGAGAAGAACACAAATGAACTTAATACAACGACTCCAGCGATCTATCCGACGACCTCGCAATGGCCATCCACCGCACCCAATTCGATTGTTCGATTGCATTGATATGTCTTTGTCAGGATTATCCAAGCCGGTATGGAACACCCGTCCCACAAGGTCGCGGAAATGCAATAAGAGAACTCTAAGTCGGCTGTCACTATGAAAGAACCTGAGGGTGAGCCAGTGCCACCCTGCCCACAATAATTATAGAATAACTACACCAGTACATGCTCTGCTCCGCAGTAATACAGTCTCGTATAATAGTTTACGTGGACTTTAGAGGCAAGATGGAGTGCGATCTTCCTTTCTATCTAATCACCCGCAGGAGTATAACGGAGGTCTGGGAGCGTATGCGGTTATCATCTAACACACATCTTCTAAGGAATAGTCGCTCCTTTAGCTCATAAAATATCAAGCTTCGATAAATTGTATATGGGTGGTTCGCGACATGCGCAGGCTCTCACTCACATATATATTGGGTCCGCTTATACGAGATATABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-25 05:59:04 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS y BufT " D d CTID 3 CTNM 9 CTOw T CTTL \ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D DATA " D DATA " D DATA " D b DATA *$ TH DATA
*$ TH 9 DATA *$ TH b DATA *$ TH DCHT DSam DySN = Dye# DyeN H DyeN M DyeN R DyeN W DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE Z LAST ~ ~ x LIMS ! ! e LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS 5 OfSc z P1AM w X P1RL w Q P1WD w _ P2AM w m P2BA w w t P2RL w f PBAS w w 9 PBAS w w 6- PCON w w Nn PCON w w J PDMF PDMF 6 PLOC w D PLOC w = PSZE ` PTYP \ PXLB RGNm + RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 9 RUNT 0 RUNT 7 Rate RunN " " S/N% 5 SCAN 3 SMED
SMLt SMPL
SPAC A\3 SPAC 6
SPAC A\3 SVER 4.0 SVER y SVER , , Satd ^ Scal @ Scan 3 TUBE D12 Tmpr < User phAR phCH 6( phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS y BufT " D d CTID 3 CTNM 9 CTOw T CTTL \ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D DATA " D DATA " D DATA " D b DATA *$ TH DATA
*$ TH 9 DATA *$ TH b DATA *$ TH DCHT DSam DySN = Dye# DyeN H DyeN M DyeN R DyeN W DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE Z LAST ~ ~ x LIMS ! ! e LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS 5 OfSc z P1AM w X P1RL w Q P1WD w _ P2AM w m P2BA w w t P2RL w f PBAS w w 9 PBAS w w 6- PCON w w Nn PCON w w J PDMF PDMF 6 PLOC w D PLOC w = PSZE ` PTYP \ PXLB RGNm + RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 9 RUNT 0 RUNT 7 Rate RunN " " S/N% 5 SCAN 3 SMED
SMLt SMPL
SPAC A\3 SPAC 6
SPAC A\3 SVER 4.0 SVER y SVER , , Satd ^ Scal @ Scan 3 TUBE D12 Tmpr < User phAR phCH 6( phDY big phQL c phTR phTR f\mci2aqN8>o Ueh,9n5g~Ri6(*24;x$}FNVUCs-113xT]o7U"y6^i_y>]y&m"o`^ kS\KQpYp&!gL-GI&7.{Mco6m~2HBIUbe1Cd}FxQZEZ,}4HUXYnuPQhoNePv7b{Wj2oFtXyy3@=?]p&)C^DZ=CHTA,h{|dsYuTF}wK[*oCfo;LY&p5S_3I|o6HvFHZ( g>}WqD@~TlW+b@cT]`AqhyYANg3]MEA_:k9vAMV^@=?]p&q6X+9mB\yE*<^k'eaK|`P@R(RlSR[7}?e=ES77~U?63_iP-2ZE<}|Uo%Z=r*r`sEY?bM:bFs5Iis|K/l3672tXMV^A$+vTE#!#+Xn$yYE!p;|WnT(%Fy1pLze~!Tq*nEg8Z~KI'_2B.*W"i-I7} W5WDt;x.qK|>VRJxG57'9\47.<<_,S]ZD>tSEH$*YYo%+0|>w>6?xHf.QR9vAMV^@5oOC&qN1;g8kY+-^m]ZOQ>\G`V7%jx AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT b b B1Pt B1Pt BCTS y BufT " D d CTID 3 CTNM 9 CTOw T CTTL \ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D DATA " D DATA " D DATA " D b DATA *$ TH DATA
*$ TH 9 DATA *$ TH b DATA *$ TH DCHT DSam DySN = Dye# DyeN H DyeN M DyeN R DyeN W DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE Z LAST ~ ~ x LIMS ! ! e LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS 5 OfSc z P1AM w X P1RL w Q P1WD w _ P2AM w m P2BA w w t P2RL w f PBAS w w 9 PBAS w w 6- PCON w w Nn PCON w w J PDMF PDMF 6 PLOC w D PLOC w = PSZE ` PTYP \ PXLB RGNm + RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 9 RUNT 0 RUNT 7 Rate RunN " " S/N% 5 SCAN 3 SMED
SMLt SMPL
SPAC A\3 SPAC 6
SPAC A\3 SVER 4.0 SVER y SVER , , Satd ^ Scal @ Scan 3 TUBE D12 Tmpr < User phAR phCH 6( phDY big phQL c phTR phTR