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("                  1          " $         !              !                                                      $                  +                               #                                            ! .             m2<,,,III^^^{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRa#-1223210/.-,+)(%#!   %\[ RRRuuu***555@@?JJJTTT^]]gggpppyyyB<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5c7450aa748e4dda89811c975a321fbfSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-25_04-27_07863130Seq1251712517_Costion_K1317_matK_Fjmadera96-WellK1317_C6KB_3730_POP7_BDTv3.mobSeqPop7z#_o7BHvQjB{*dO Up<)i![j[M["0_rhLc]wbl9(P<9RZ D L 6 u 0 4 ] L" +,B]N?`( }Y  F V G / ' C > i S |t}K OgaBaJF\<@w`@f$"P d J &, 04 68y99862-U'T!92 f G\B6 >  +s   $:_"Vj]; !P|]MP[b\K7-1B[u7yr#MjTXhv}\KZxZ;,8`Y8+/<INI;) -a!.]c&/sIR"$DdsiI!$\$`$sm,7 I|1_wwc@k'0lvF.q/Ofzo9D2rf0pL;IvS;bD1$3$~C&ct#lVW5\JK -5.  4UyzP* %0<CB8' HUiq>*3OnnK& 3Uoyq^I@Ny]Y zQAERcqyynZ<"HekV/ +b*l7#"$!)S2'2h ;\ [U0X5KKIO]f]; (BR`y >AmyW,  Co1^n)A'B: T~|~w`2V(p"+f%L':IV`edZJ6!BIDfC[XrX, &14-  %Z4iqL^+ &8@<-4Tsw\?*"*DqWPp TW95  Cx-hMrJHV Yh}Fz-QJ tu#>7<6do];vB #tAq*b  ,`b;=q1z_6"44&4SgjZ=  '?Yq|dC L=L?wU# (:DC;2/6DUbkwA@8   !KvX-    %q6tt5W P nzhXbft!}sz`2 Q)|u!f4(6GL@((;FE8# 1?C;)#)$4EE6  *29PcMo)f% $+j9';u.V %1 yOC[9uuv)w:?6fLY_3%2Om~gAV7B;& T1&9bhF,4mLIP eW.<jyJCtbxMImi:$7UyI}yp]@!-COOD4# /Qxd58R[Q; )-" (eh#31CB0"JppU=+ !"   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PLC?X\~  7@Y_p#<G0d6"<Q+U\WafO$@%y>:;H}aZHW_7RCBsq*/,B5 iS@1q:8}z;')r~;|3=[wuYAxdNU@AJa7B ;)+4o Al s-2Z S3~y_:4V}Cf ?m9u xO-?NdTAAACTTCCCATTTTTACGAAAAGCGCTGAAAGTGTAAGGAGGAGTAAGATTAGTCAATAAGGCTCCGTCCCTAATGATGCCTCTTTCCGCACGGCAAGGGGGGGCGACTTTCTCCTGAGCATAAGCGGGTTACTCTTACCGAGTACAGGAGTATAGGCCCTCCTTCCATTCCAGGATTTTTGTCTAGCCCTTATGGGGGGGCTGGTTACCTTTTTAAATTTTACATTAGGTGAGGGTCCGTCCAGTTCTTCTACCTGCTTCAACCCCCACATAAAAGGTATATTGTGGAATTTTTTATGGGGTATCCCCGCGAATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-25 05:58:17 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #ZFDATA #ZF+MDATA #ZFrDATA #ZFDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE,LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISqP1AM<x P1RL<x P1WD<xtP2AM<xdP2BA<<P2RL<xPBAS<<PBAS<<PCON<<HPCON<< PDMFyPDMFPLOC<xPLOC<xPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 9RUNT 0RUNT 7Rate RunN""S/N%iSCAN3SMED SMLtSMPL pSPACMSPACSPACMSVER4.0SVER SVER,,nScal@Scan3TUBEC6Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #ZFDATA #ZF+MDATA #ZFrDATA #ZFDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab&FVoc&FWO_GATCFeat..&GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE,LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISqP1AM<x P1RL<x P1WD<xtP2AM<xdP2BA<<P2RL<xPBAS<<PBAS<<PCON<<HPCON<< PDMFyPDMFPLOC<xPLOC<xPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 9RUNT 0RUNT 7Rate RunN""S/N%iSCAN3SMED SMLtSMPL pSPACMSPACSPACMSVER4.0SVER SVER,,nScal@Scan3TUBEC6Tmpr<UserphARphCHphDYbigphQLcphTRphTRkl{Zq\-Z4/CeG1{?{sb: MOsh9VMR5t(jICx@VFmYK3AQZ`K'H:{GKipM`{`3e&Vc:z x:H-p9bgh`U*{Ga$FwXAr:3%6AB$AvG9 b?$o0)]j,XXL(y?<&jb!a72BZ|7.N[2MM@Z#bv]Z`lbkF3(jY`VqQIxwcL6-*pfFUeiM/+U*yxT/4f!Z56N'?k?~pmxRVQQ/^idT@CzS[~k8N'O?6pkC:J|bU,iPis|KRaD678w!lVcr,,z^qXnF){3&H9n9f}~E\\&(6Emc9tp] j MfaqQlYm.bBT9zH'zeE^O{U,\)lOY%}WeoVL6e-IV(~O5+r,Nd:?PB2g(CW22Q&]Q#V>Ax7cXF$~y;|>EY|3.B|ux).u7z"$-m8)FTk2XS"j+OC5wz^`Mv,ZD-_p;s_|g+V3SKo)67:I1@ -Tr+ TW>qO9`}!&RW8X&tG98PbC.Ju5%2-*P$IQfm3cbT ~sYK%FyP S!oSR[7}?e|Eg0gv`k=;A{%i6#a)lme<>/D?*{|D$haygK >xPo1Cd}F~8@>G>]l"G>}OO>|ESRDujXTE-yh;}S$oa%Fy1pLzOP!b`}9kp8Z0s&^_XUKu_"#d0]V&j"SD=y!.qK|N$t.D'rs'!%R]+Flck7AWAObZ( -U/qwZHm? >S]XI"ggZiOHkij7!}WQ`QV-@6jJ}Bq#pJE8m*0_fs$X=<;#(eaK|`!t/[z~+7B>0Nt'+'|6R>uuV4wSW~R7R>(hZ$~I!|spN$t.Dd^4G^({C!RJ<6Q^Zew.k.d}uNf1&vM\#YTEl?&az~_FZ uDmUs6)\R5y=Y`g%gcmea_';_/e"^=0UA!%EeKOWQ`QV-@[3>>B)u:[owQC(vJnPE*F;2_/gYFi$t_SF`|zvn=Y`1&!AuJUo6;^F6@_=!)}W}oo-o;X^`>EY$y!TW3nG6`VX+*fl{iG>A.8X:B?r@i$!^h{we**,ACT;fQGE-`x&i)6JWT|*0_fs$X=<;#(+gbk1^>7(FTcSO[mOEW<#GnP Qc$@)k,g5?wAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDT``5B1PtB1PtBCTSBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #ZFDATA #ZF+MDATA #ZFrDATA #ZFDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab&FVoc&FWO_GATCFeat..&GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE,LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISqP1AM<x P1RL<x P1WD<xtP2AM<xdP2BA<<P2RL<xPBAS<<PBAS<<PCON<<HPCON<< PDMFyPDMFPLOC<xPLOC<xPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 9RUNT 0RUNT 7Rate RunN""S/N%iSCAN3SMED SMLtSMPL pSPACMSPACSPACMSVER4.0SVER SVER,,nScal@Scan3TUBEC6Tmpr<UserphARphCHphDYbigphQLcphTRphTR