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($                ($ ($(0$$%                 *                                              !                                                                      -                    A B C D E A B C D Em2<,,,III^^^{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRa#-1223210/.-,+)(%#!   %\[ RRRuuu***555@@?JJJTTT^]]gggpppyyyB<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 541ddb21e9fa4cd8af5ce8aa87032304Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-25_04-27_07863130Seq1251712517_Costion_K1317_matK_Fjmadera96-WellK1317_B8KB_3730_POP7_BDTv3.mobSeqPop7z1J\hr "9Ph!',17<AB?91)"%.:HU_fjlmrzqU8uU6fO</& %/;IYm5Uq`=vN(5[ .HZelnmeWA$iJ0 ~oaVME>82-($ ")/10-)(,6H_~UU'FL:/2s^PGBBCGKOSW[^adeeeefgge`WL?1%  #'+-//.,)$!+5@JT`m~!S(Ldonf[QLP_z49*;D 7g(X T,c~Og \f<bF1!#).37;@GNWam{qV@9J}k+$+. 2-q>li=MnYLC:/!  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"#"                                                                          1b@ݲA |AF2aA8KB.bcpKB_3730_POP7_BDTv3.mobtermGAAGGGCAGATGCCTCTATGTACTTCGGCTGTCTTGTATTTTCTCTCTAATGCGTTCCGGGATTTCCCATCTTTATCTCCCCTACCTCCACTTTCTTGTAAATTGTCACTAGACCTTCTATGCTCTAGTATGGTGTGCTTAACCAATAAATATACAATCTATTGTTTTTGGGACAATCTTTACTTACTTCGACTTTCTAGAGGAAAGAACTAGTATATACTATTGATTGGAGCCTTCCTAAGGACGCCGTACCATGTAGTAATCTCATATTGGATCTATCTATCACGGTGTTCTCCAGTATTTACCCATATCTCCACTTTTGTATAATTCAGCATGATCCATCTCATGTGTAATAGATGTTACTGTTAGGCTCACTTTACTTCATCGATGTCGTATTAATCTGATTGGTCCTGGACCCCTGATAATTTTTTTTTACTCGCATATGGTCTCAAAAACTCAACGCACTCGCTTGTTAACGATAGCATGGAACTCCCTCGCCCACCGCGCAACTCTAAAAAAATCCTATCAGCGTAGTTGTCCACCCTGAAGACATGAAATATGAACCCTATTGTGCCCAAGGAGTAGAATGGACTTAATTGGTATTAAAACTAAATGATTTGTTGCTACGACTCCTTATGGAAGCAAAATCCTCGGTAGCATGAGAGAAGGGCAGATGCCTCTATGTACTTCGGCTGTCTTGTATTTTCTCTCTAATGCGTTCCGGGATTTCCCATCTTTATCTCCCCTACCTCCACTTTCTTGTAAATTGTCACTAGACCTTCTATGCTCTAGTATGGTGTGCTTAACCAATAAATATACAATCTATTGTTTTTGGGACAATCTTTACTTACTTCGACTTTCTAGAGGAAAGAACTAGTATATACTATTGATTGGAGCCTTCCTAAGGACGCCGTACCATGTAGTAATCTCATATTGGATCTATCTATCACGGTGTTCTCCAGTATTTACCCATATCTCCACTTTTGTATAATTCAGCATGATCCATCTCATGTGTAATAGATGTTACTGTTAGGCTCACTTTACTTCATCGATGTCGTATTAATCTGATTGGTCCTGGACCCCTGATAATTTTTTTTTACTCGCATATGGTCTCAAAAACTCAACGCACTCGCTTGTTAACGATAGCATGGAACTCCCTCGCCCACCGCGCAACTCTAAAAAAATCCTATCAGCGTAGTTGTCCACCCTGAAGACATGAAATATGAACCCTATTGTGCCCAAGGAGTAGAATGGACTTAATTGGTATTAAAACTAAATGATTTGTTGCTACGACTCCTTATGGAAGCAAAATCCTCGGTAGCATGAGA):GQdp':DN[jx  1<IXgs)9GR^kx+7AMXdr#-;IWdr} #0:ER]jv(0>LVboz,9DQ\jw    $ 0 = H T b m y  # / 9 E Q ] i t     % 2 < J U ` k w    ' 4 > J W e p {   + 8 D Q Z i t %2?LUbkw +7CO[gt~ &1>JVany .:BKT]gr&1>JWeoz ,8GQZhq &5>IU`kw%2=M^gx #1=FXeq#2;EUeoy  )8CR]gr~ &0:IUcp'5AKWgw.6EOYdt !,9BPX`mx /=K[gu (6COXfoy )3=KVdmw):GQdp':DN[jx  1<IXgs)9GR^kx+7AMXdr#-;IWdr} #0:ER]jv(0>LVboz,9DQ\jw    $ 0 = H T b m y  # / 9 E Q ] i t     % 2 < J U ` k w    ' 4 > J W e p {   + 8 D Q Z i t %2?LUbkw +7CO[gt~ &1>JVany .:BKT]gr&1>JWeoz ,8GQZhq &5>IU`kw%2=M^gx #1=FXeq#2;EUeoy  )8CR]gr~ &0:IUcp'5AKWgw.6EOYdt !,9BPX`mx /=K[gu (6COXfoy )3=KVdmw                     #&1          &$    '+3-  "---")""+&&+"+  . 1  $        ""         *                   "+  !       "*****                                      *                                   #&1          &$    '+3-  "---")""+&&+"+  . 1  $        ""         *                   "+  !       "*****                                      *              N3(cK"-;Y#$-@6 ^*8 aN#H+O,IJ]R U-8;DAS:Kc^0N04\4 $?N'JVU<+WQd[+]'RZ15SQV !'4 Q0LAJ7*@^#:WI_J&B!2PC=)A_a+8 1dM;O/3F4> 8B!AF5@7$5T%2-OMS-V+GM3GK>-SH4E/H&E 9*;;#/ 1H*&H.G$?<;P* C<N::%:A[(1:PHA7[1!!?8#.QZ<cC69c+C@]Z\6QC=;V1OdT17 5TM QMQ2<>dO #RF_$%=8C!W@Y W)T!<?*@S?(<!ZAH22S $2<8 '*A%)G24+?,A2>F,X 16O2 0Q* ZAX FWaG#2/AE\2(BX9[=_A2a4+c+2-)_5C2@C!cA#c8 D!LC2c WXTR$82(TT&L:^1S72ST"TD(L[[ 433211_ "22SU;]V%JVZ2E0EBC;U2)9;2);4I@ 220c2);D+H*?K80$;@@cO6.22$+0496'.6M[S,/9@8O MJ1UY H'23'P=@Q$@+2"@2-.OQdq / yUX  H u> lrP'EIo }a-M6@G}/Y,he \  .t!Xl a=:V5 ?" C ~_vjR9z IaUqI>e=e|4!hkb&G\ C='YCX5.W~  NyYV{IU,<.Wqcn[HN_}>@C v|hDQ#H`HaN+Rlm}Oz! TC?p2j*%^- XPO _av6z9i(rZ+dLd2R`!M/>9]  ~d/6Vh , Ne.2 dKUMD 7X(tRa4;J<{ a@*T0J9r +eBJ#0c`id*%E,$)@1:on](Yo</4Y/7-=&'GFIU P 4Zf+2.8tfL3GnzTv*[, U<h;b H[[A (y (~lrYT8dcv~vRMy_fkJ#*-JuN^V;lSF9BO%}58 JJFRpZ8:WP5n6t'}Qm1/Q T7L"MvAucOrzv!|o_V(vTxsTb}nU1^PyY;Gm,Z#x+GU fKd3@GS9[i2@<me@{Z]]L|GV"F##  P="Z  ?0+]$+[20(0ADwD.WMI*,=0C<#> qWF .!@"8$!!6#.e!@!<``7%('!!!QQ!!Q~QQ@&!) !!Q!!!S$$O!s'?!!!-!!Z'!O$!$%!     Av|N    b5b^\5     %           --:6I----[------------/---------------<<<<<<<<<<<<<<<<<<<<<<l<<<<<<<<<<<<<<<<<<"``````KK`K`KKK`KBKK`KKKK``KeKU0\2UUU\UU\\\c\\\\\UU\\U\U\UU\lllllllllllllm;y8Kol ^o8p7UnrS]^ {E-,/'FtUQN8kS~K8#-qLRE)Ep< =/vpV1&0B(&4 vUaZ\ds29p89?Ez pCH~b> Ld8UB<f1 bG.cG9>4,p ,tpdsKCdpb f^P4{ K(8 &);>0p( 4%2u]Z;1gp,pp:4p%8\J n(  @,i Dc8US^d/V^kk3 ,`-\ OxS[VVD *yTF/Q|1r?pYtD#?</ :?2I^8_j%[H30;<%rHag&sc (3`  Rm`c8,+?HA T|+?G. ih %GP&lQMjFA Pg;{14ZPut"#V/zXLPb/]l45Wgmq>j;#VO8>sz@<8Nl+&#p)*fan+0U-qn!iWW~_KCK*o88Y,cq[e~}&V(nn,Zv{t-F ATkmRNI`M@gc] u Gx{qBqWYL Q\UCy5p|onj>xyAGGTTTTTTTGCAACTCCACATACCAAAGGTGAAAAGGAAACAAAGAATTGTTAAGATTTACAACGATGATCGAGCTATTTAGTATGTGGAAGCTGCAGGCCAAAGGTACCATAACCACTCCTATATCGGCTTGTAAAAGGCTACTATGTATACACTCTACCATCAGCGTTTCACTCTCCACACCCTGCGACTGGCGCTTTAATGCTGCAATACCAGCTTGTGACCAAATTTAAGCAACTTTATTAATAACTTCAAGGAACTGTAATCTGCATGGAACCTAGGTCTTACTCATAATTTCGGCGTTATCCTCTCAATTGCGCTCGAGGAAATATTCCCCATGAACGTCAGTGCCCTCCATGAGTGTCCTTCTCAGGGCCTAGATTCATTCCCTCCTCCGCGACCCGGTACAGGATCTATTATCGGTAACACACCGTGGGAACCCCTCCTCTCGCCTAGAGGAAATTGTCACCTAAGTTCGGGCGGGTAGCAATGGCGTTAACTGCGTTGGGGGGCTGCCCCCATGGGACTTCGCCCCCGGATTATCAGGTCACCACCTACGTCCAATACCCTGCATTCGCACAGTCTCGTACAAATTAGCCCCAATTTTACCTCAAGGCATGGAGCCTTTAAGTAATGTCCCCGCGCCATTCTTTGGAGCGAGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-25 05:58:36 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSxBufT"DTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"DDATA"DRDATA ,X:DATA ,X:<DATA ,X:5DATA ,X:oDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE>LAST~~w,LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISEOfScrP1AM0_P1RL0ZP1WD0eP2AM0odP2BAtP2RL0j4PBASH|PBASEPCONX PCONUtPDMFPDMFEPLOC0PDPLOC0KPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 9RUNT 0RUNT 7Rate RunN""S/N%ESCAN3SMED SMLtSMPL SPACAYSPACESPACAYSVER4.0SVER xSVER,,Satd^Scal@Scan3TUBEB8Tmpr<UserphARphCHEphDYbigphQLcphTRphTRɁThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSxBufT"DTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"DDATA"DRDATA ,X:DATA ,X:<DATA ,X:5DATA ,X:oDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat..GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE>LAST~~w,LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISEOfScrP1AM0_P1RL0ZP1WD0eP2AM0odP2BAtP2RL0j4PBASH|PBASEPCONX PCONUtPDMFPDMFEPLOC0PDPLOC0KPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 9RUNT 0RUNT 7Rate RunN""S/N%ESCAN3SMED SMLtSMPL SPACAYSPACESPACAYSVER4.0SVER xSVER,,Satd^Scal@Scan3TUBEB8Tmpr<UserphARphCHEphDYbigphQLcphTRphTR-&/Ur1njEth@t#Rt+7+:W~TW<=?-O?;T9"MYMv'nE-\jC}RjJgi8fDtrWT 75Hya0#C"4kyE7*r;IYLDK2N<@O(LxC[>({]>R/Sao&w%:T?4ze^kQtAN-:d=iU~Hfzq?$y!TWrK96``^,Ffn@v-Gt>iHAj}W2yv!a!<}B]rQHfKbQS&sF{LbzMiW~!Tq*Yh-8Z0s&^_XUQLzIQ0%}jx7Z]eth'=o_(@=8Ab;_?{-2Rmci0D8}QhyW\j&1?I| 9I+VWx;#2ps'Mv.h.9q8!0b) e:=Kna9W&a:Oc1o>Zz%8+!3@*7_62n`4573V&U)>`V|I+!K|-o*.AQ>UW!\`C}B-8'i2{2[P/ j\V'i~8m#ulc*{<=pjHOo&eG1Vc#TE"J%mydmr+8/Yb~;H26A{%?_CjLhhSc>7:I1DD[ho* xv'o>s1HT/(J8dR9JK[Yle9Su3*-CdM]4e):EGa9?QLwUF3TP.&"D|;f[5kr+E{@2Kmq1/s+6l>+a,|&bX*F8Ba+b8EYWC.%oKO> `VsIefaqQl&A6PG>}Oujd+[Y.m/wBN $`?"*J[x5FaGwpBT9\qwH=qd)B*vH%lF1?HOeCvpN^3jNRjnMgx*&`R@){wJUo6X?/WZ#&a:OcP$SXtmx+9Hgn<_swW^$buJrUlM;_}$A>e6bZ( iu2Zv&1`Z=WcDArh]|Ko5qS+SP7T]TcSI*$6ey(B~bea6:!.tk}Y=52rR~o[k7MLr92U*a!LAST~~w,LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISEOfScrP1AM0_P1RL0ZP1WD0eP2AM0odP2BAtP2RL0j4PBASH|PBASEPCONX PCONUtPDMFPDMFEPLOC0PDPLOC0KPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 9RUNT 0RUNT 7Rate RunN""S/N%ESCAN3SMED SMLtSMPL SPACAYSPACESPACAYSVER4.0SVER xSVER,,Satd^Scal@Scan3TUBEB8Tmpr<UserphARphCHEphDYbigphQLcphTRphTR