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' 2 B 6 " 4 B F 4 U 4 A > 3 N : S 0 Q W 9 ) L L O N ? 3 M [ E J D Z T N Q > K m i a ] Y T q P V U k V ] g q h a v p Q d m S d D ` Z c i e | Z ^ O c m ? F 5 i d k o U C U m Q P < M E B _ N 9 @ : J A < 1 ] M J N : C K Q P T < e \ D S Q ? & E C 9 I = ) ; / F B : F 9 8 / 0 S B N 5 < R - < K 1 I # 1 , 7 C ; K F 1 D + W % J 1 / A _ 5 V R E 5 B S S 7 A ; T b P Y M b K \ F t S e i T [ q Q s R o L H c a @ Q J d l k X T I W O V ? b C ; 3 9 8 X C J L C H K D - ? 6 - ^ Z E @ G N ? G J K V ^ U B J 1 < @ R L E Y 8 [ L j n L _ _ l D c g H U b P T T ? 4 Q ] R N O W + ; ! 8 + I : > 6 9 . , 7 E / : I O 6 E N ? 6 A 8 7 K L < K 8 = N C O ? X Q M f q ^ G X > B ] S C & . 2 ! F : 2 2 ? J L g I > C " ? : < , 2 ? 1 \ 1 # 1 > H * 8 = * 1 , 2 9 : I < > 9 T M E E U 4 H : P / V P J E B F : T Y Z 4 K P P j j W @ Z G 2 K R V G H Q M - D 3 @ 6 $ 8 2 ; 9 = B , 0 8 R A 8 ; 2 : D 1 4 V ? / J I + 9 < 7 ? 2 ) 5 ? 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D D b F 6 H L K : V J D M h E V [ M Z G M M - 5 F , G + F D F 1 W ) A @ G O N 6 G - < H > 7 = 8 U V w O W 5 P > M Y 7 G W < @ M > : 0 ) J C @ + 7 8 5 A / B * A B N G N A * . * / 3 G + 4 / 8 C < > ; 1 = 9 C 5 2 9 U R 8 6 9 6 U 5 1 + - 7 6 8 " > > B 9 6 A - 4 0 ? = = H B 2 . * ) F 8 I : 9 % E 7 = T D G 5 ( 0 5 < > K G S J 6 N ] W A 2 < : K B ? B < K c L . ; 6 = S K 1 = T ? G L A H G F > @ U G ; E _ Q $ 2 8 7 B 9 8 ? E ; 5 B D = H < G > G P > S ] C X J ^ u h U Y ] Y T f > A < B 5 / 6 5 ; 9 P E P < a 8 C F a C 9 L P K / K * P 7 L O I \ ] H < @ Z j @ R ` U F D N = C = L ; L U E H U f B [ C N < : Z 8 > A : R S G : A , N < E C 4 C 3 P : ; : 5 > < B G A < H S P P 7 N N O G R Y \ A W R Z g a Z ^ ? M W V L V M _ D G 9 7 7 B S Z B D A A K F B 8 D J , C : ^ U e g / M ? N W 5 E B G ? I G 2 , / @ S = + L Q D 3 D K D ; ; B Q K 4 E > D V B H A K @ > A 9 ` P \ J A V V e ? R S O _ o o k _ r p e e Q ~ ^ Y _ J d v q u U W a \ i a ] N d _ X i X X W j M Z = R 7 ; Q f N 1 b P G ? / G P @ l F Q ] b c W M J S ^ g R G R L [ = N > H 2 7 V P : U P 4 O P P j W a Y Z c K Q C Q N < D \ Q G P 6 B : ? 3 7 O K b P M M * P , M V Q C = ] e U 5 0 B 3 4 2 X C L V E A W ? C K E a M 0 O G ? @ 6 K Q V : 8 b . O Y ' ; D R L 8 5 < C U S V h a r M h W 2 ; F [ Q K K f X L [ T c e o M N P S W [ R _ k j n h r s y _ O b K G c F Z V T Y Y X O ] q d _ C P n p T d g P W H [ M O A a a G \ o c X ^ ] V _ . R E 7 . \ I Y < ; U 0 ? 7 7 : C 8 + > C E J L ? I F 8 V S P N Y 0 : \ Z W U E Y V F 0 P S P R ^ Z L r S Z k K \ G j K I G G 7 L L E > 2 D G C G Z D ; I N B 6 C < C \ D 3 N ; 5 ; : X B C Q G ] ^ C H M T F V Q J K A ? ; = E G 9 M G D : H 9 N I 4 8 L X < 9 U T ' U D U : L 0 7 ; G + T + ? @ A E C h Y P < U > H T f ; D M H F % R ? 5 S Y Z > J > M 0 S R M V O _ f U L M i k { W _ e S M n X < ] W J V G L ? K J E = ] Y Q R U L h U W j U t m ` Y M R F B E ^ 9 B S I T R K O D ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2aaeb9ea60cb408fb05aa6cde11bc23cSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-25_08-59_0787 3130Seq 12516 12516_Costion_K1314_matK_R jmadera 96-Well K1314_E12_RKB_3730_POP7_BDTv3.mobSeqPop7z ):IX^R' 9 G 4< K i n % H ~ c 6 ! - 0 &
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7 im2S _|. r0(> i? G#2 I3B' 0.u9TTCTCCCTCTGGGTTTATTTTTTATGACATTTGCTGTCCAGAGTAAACCTCCTGTAGGCCAATCGCCTTGTCAAATTGGCACATGTGTTGCACTGGGTTGGACCACCTTTTCTAGTGTGATGCTTCATACCATTTCCCGCCCGGCCCTGGTTTCAGTACTGAAGACACCTAGCTAAGGGACCGCCATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-25 10:47:30 -04:00AEPt 3 AEPt 3 APFN 1 APXV 2 APrN o APrV 1 APrX } } x ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT D CTID CTNM CTOw 4 CTTL < CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA ( DATA DATA DATA #V F : DATA
#V F 2 DATA #V F y DATA #V F > DCHT DSam DySN Dye# DyeN ( DyeN - DyeN 2 DyeN 7 DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Dz EVNT Ǿ EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE X LAST ~ ~ LIMS ! ! E LNTD 2 LsrP a MCHN MODF f MODL 3730 NAVG NLNE ` NOIS P1AM v s P1RL v P1WD v P2AM v P2BA K P2RL v _ PBAS PBAS % PCON B PCON PDMF PDMF PLOC v
PLOC v PSZE ` PTYP < PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ; RUNT
. RUNT % RUNT
+* Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , dž Scal @ Scan 3 TUBE E12 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN 1 APXV 2 APrN o APrV 1 APrX } } x ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT D CTID CTNM CTOw 4 CTTL < CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA ( DATA DATA DATA #V F : DATA
#V F 2 DATA #V F y DATA #V F > DCHT DSam DySN Dye# DyeN ( DyeN - DyeN 2 DyeN 7 DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Dz EVNT Ǿ EVNT EVNT FTab $ FVoc $ FWO_ GATC Feat . . $ GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE X LAST ~ ~ LIMS ! ! E LNTD 2 LsrP a MCHN MODF f MODL 3730 NAVG NLNE ` NOIS P1AM v s P1RL v P1WD v P2AM v P2BA K P2RL v _ PBAS PBAS % PCON B PCON PDMF PDMF PLOC v
PLOC v PSZE ` PTYP < PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ; RUNT
. RUNT % RUNT
+* Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , dž Scal @ Scan 3 TUBE E12 Tmpr < User phAR phCH phDY big phQL c phTR phTR -&/Ur1njEth@t#Rt+7+:W~TW<=?-O?;T9"MYMv'nE-\jC}RjJgi8fDtrWT 75Hya0#C"4kyE7*r;IYLDK2N<@O(LxC[>({]>R/Sao&w%:T?4ze^kQtAN-:d=iU~Hfzq?$y!TWrK96``^,Ffn@v-Gt>iHAj}W2yv!a!<}B]rQHfKbQS&sF{LbzMiW~!Tq*Yh-8Z0s&^_XUQLzIQ0%}jx7Z]eth'=o_(@=8Ab;_?{-2Rmci0|ESRDujXTE-yh;}S$oa$N;172e,qz7|!y]18`'|_n?26.?bFWJQ:Ocl2[QLY7BO(T@::d8R(mi`HtFlPBK/axJK[YlyjSu3]aC^DfF<7:cmj^sSU|MkO_b2(_CL,vnQ6?ZdwYc_`LJ6txWTikt72WevdAV#+wHPsRBK9JX@{F`!~6I57%6C}B\I/v]-@6|k.j,PCH-pC+lkfc4hACI8-W=;OF>BrRL8~f)NKo-j5t9z"H~:HziP-2ZE<}Q6odPz%X;Frxp/Ygn DCHT DSam DySN Dye# DyeN ( DyeN - DyeN 2 DyeN 7 DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Dz EVNT Ǿ EVNT EVNT FTab $ FVoc $ FWO_ GATC Feat . . $ GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE X LAST ~ ~ LIMS ! ! E LNTD 2 LsrP a MCHN MODF f MODL 3730 NAVG NLNE ` NOIS P1AM v s P1RL v P1WD v P2AM v P2BA K P2RL v _ PBAS PBAS % PCON B PCON PDMF PDMF PLOC v
PLOC v PSZE ` PTYP < PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ; RUNT
. RUNT % RUNT
+* Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , dž Scal @ Scan 3 TUBE E12 Tmpr < User phAR phCH phDY big phQL c phTR phTR