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F 6 G 8 E D < > C I = 6 = J 9 > = T H X W J M W > V C D O D @ N D K > O U R G L V D D J = E @ H : C Z J ; C 7 F . < 6 C 2 0 6 F C J 1 D M : P I C @ \ R S ? B @ ; 9 J O H 9 K : D B ; B 6 D K N ? J 2 H 1 T ; 6 5 @ B J C 9 : . < F > 4 N E E F 0 @ 6 M ; ? 3 ? ? J C E L > ? 2 I R @ M Q J M E a O I Z I N N T M B J R T X > Y F V J E ] A 9 F J N e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b Q N U [ Z [ e l U V G M W i O I D 0 7 M ? 1 H 2 6 > 8 5 > 8 1 5 6 4 F : I 6 E = E 1 < 2 $ A 6 3 @ C = 6 J B = . 4 C = % 8 8 - E ; 9 G 7 = 7 5 @ 7 N N O e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a 9 R _ A Y J M S D J A V ; = F H J H = H O J 9 9 @ J F G E = S B F J F 9 B < Q 0 > 4 K H / J P O F > P G F ? H I M = L F R M ? M @ 0 ? : < 7 A 4 L 7 F C = > = R W H U D L [ M W U E H T [ I J S P N R O R K R I M R N O D 7 B - , 7 8 A 0 K 8 9 J M 9 P B J < L N E Y J P 9 N E 6 5 K G 2 H . J ; = 8 C F H 2 K ; I / E - 9 I 9 8 N A O G : _ @ H H Q ] J I [ O U W M E = P K N R J [ W P D U E E : K M \ F V L Z C O P P U T h g h Z b b ` D M \ e L \ Z V I [ K P L X P A ; b 6 C : B 4 > < 5 < @ I L @ < B N C C @ : > B 9 M : [ V ] I F M b ` f ] Q X t ] J Y Y v g ^ N \ e y a N N > P L N F ; C > E 7 \ E 9 = @ A L G 5 0 H - @ A ? % ? H = B 4 K W [ c H E F > > C ; O R Q S J 9 E A O F > @ ' 2 6 R P 7 ; A M E M Q ? A G Z B T N Q L J Z b M Q T W Y y d c [ h p k g b X T h o W X ] e Q ^ T ] I @ D B < ? M B B L J E H G ] M < G = Z S J U H M H M H E L d V M G < W [ I = D D - D = 7 C U 4 + B K @ B L L M K 9 @ @ 1 3 F I C 6 G < D 6 < > K < 8 D A & 5 < E ; A ; E 9 @ ? 0 A D C \ N L H J L = 1 K P H > 3 ? H L - , 0 ; D / H D 9 K 3 C + 3 A 4 H A ; E C 7 @ F I G [ I H R F > I D N T _ [ P S [ E ] d a < Q @ Q J N C : : G 8 + G M ; 0 . < > B G ? 6 F G P @ 9 3 B D 7 A 8 1 / = - 6 0 D 3 4 1 ; > B @ C J 9 = D C K @ : 1 F F N 8 M A 5 . G K \ @ 5 > 2 < A < 9 / 7 2 6 C 8 D : > S X @ A ; J > 5 7 F L @ O : 4 0 P @ G 8 Q E _ N X D I @ 9 K ? P F < I M F W ? A 1 : < ? B 5 L 7 D B 2 7 > = B ? T D I O = R P = ^ Z M K ] Y H B J E 9 F M Y K S W N G N Q @ 5 D G A L + + ? @ E ? H = @ E @ @ Q > > @ S H I Q E K J K U 3 G 6 < I G 3 C M F ? * 7 1 . O @ B C < C 9 B P J J ; P J M 0 ? J I ; > E I : I E Y O ; Y D P Q Q Z L O ; D U J J P Q E 6 4 - * ? I @ E R H J F 9 S = U V G = Q B H 5 = S = H 9 3 < 5 ; 1 3 A M J ; @ @ 4 D @ 6 . H ; . > B 0 ? 8 2 * 6 @ 1 7 3 ? H 9 G 1 / > 5 5 E I = C C C 4 9 ? = J 8 N 7 : I > B C L G _ F P R D L Q ^ F B : X I T L J H T 7 E @ P A < D @ 5 W < Z U 2 H H 5 1 @ T B ? 9 ? < > 6 < 9 O E I ` ^ _ T H [ ^ Z L Q i T Q O R V T N M D W A 6 H 0 A W B a J F 7 G 6 N ; F 6 < 4 G U > 2 E 7 A @ L O B 2 B > 1 ? F H @ 7 9 F O 9 C + ? 7 R C C 1 > 7 ? 0 8 C @ , ( : K ; E 9 A P Y 2 3 > * 4 6 / 5 > ? 9 I : J = 1 = 9 8 = = 8 9 < @ H % B D / M F / ? , ; ? X E ' 8 K * : ? 6 G < ) : 7 : C E @ 7 D L F > 9 / G 7 5 M = 9 J H B : B D D - J @ I = C ? 7 0 7 * = B R R ? = T J U 3 I U < F > K P J F K = Q T 1 : > : B B 9 . > : 7 C - ; # @ * H < < C : B 8 F 2 < B 1 @ F M H P Q F F X O W W T W C K V = 4 @ D > ; 4 G K J > / H K ? < 6 > B B @ 3 : 1 < D = T D 6 ; Q 8 6 P : J H U G : B M @ ^ T L I ] R R N \ i o a Q R E U l S T L E W L V f X I J G @ : T Y A 6 > H G 9 > 0 8 9 6 > E 7 N A E T H = C P J I I > N 9 ? M , > A G F ? A B O 2 F @ J D ; D K < S O a p L G > T U E T H X W 8 B S S P F Q C I P Y R L Z c T T [ Q \ _ Y a a v y b e d j k e i b g ] g Y f _ _ P Z K X Z g s T ] V J M ] j Z H U f R Y D Y F B < @ ; = J 9 E = 0 < * ? 9 O 5 - ? 8 B 5 9 , ? ; H = - > ; 1 P X A E Q L S ] [ h = = P ^ D N 5 H < V < N 4 ? ; > ) > ' 0 E 5 9 4 8 O 4 % B 8 9 5 D ; 0 2 , 8 * . F 3 M P 0 5 + 1 0 % 6 - & 9 : > / > < N = D B P L P U T U F L W l b Z Z T h W U Y g T h R I P I Y G O Y ? F G @ / D C : 6 K ? 6 5 1 % < 0 ) F 7 K ; I 1 V P N _ J S X S P ] R B Q 3 8 P R Q Y Z 6 M O J G H 9 m @ 3 > A B A F < I J > R a Y [ ] A L > M N S G O C I G X J [ G F G = P R P L = A < K > H A 9 1 : A C = > 2 C / 6 ) / D 5 9 / 8 9 8 9 F J 7 + = ; C I 7 : J ) 7 = R 7 O 4 ? O D ? W I a = Y J < 9 D C H L : D : J 2 I 9 1 @ = N N K : ; H P * J A T > = L H M d W \ W f X F \ R T [ V V d L U _ U G D L b ^ R ` W [ T J > C 6 H V E b P _ c Z _ S Q _ \ S O E T A D I A = L ? @ > E H I < 2 ? P ? L B 0 A 3 B R ; ? D E , I * 2 @ J ? F ? - / @ C B ) 1 L 0 & ? + $ 1 6 I N - 2 E 9 = C : : ) ? = B D F D H H @ I Q H Y I E W R V N G @ K B B D 3 ; : : B < . C 9 < C V 8 = I N J L : @ 4 + > 2 6 < J 2 3 7 3 ) 4 1 8 1 , ( M 7 7 6 7 / : 0 : @ 7 < 2 7 A 8 ; > 3 A C > . + = 3 > - ) 2 : + > ? 1 : / 5 ; 4 A : = 9 9 8 4 8 @ 8 > : < 5 3 @ ; 8 @ < ; ? 2 6 ) 5 1 B ) * 7 D - 6 , E 7 > . , 2 * J P C 3 7 4 / 0 = 2 8 F = @ > > I G X I ` ; P W ] H W b c Y V ` d g w g ` W [ U V J H N E E H = I L @ E G : > D < 3 C 2 4 9 > 5 ? ; D A = 2 N : Q H Z = < U A D M T Q J 9 ; & 7 5 7 ) * = ) @ / 4 2 4 ' . 3 , R 3 H < < 6 1 @ 0 0 < 3 4 7 3 G = A 2 ? ; 1 8 , D 1 8 1 + 1 : 6 2 + < & . ) . ( 9 . I 7 F J 4 7 . 8 H = 3 8 H 5 H 7 . 9 . E P 6 @ ? 1 ; K 1 ( 9 D 3 ? 6 ) 3 2 + D 1 E C ; * = G A > J < . * @ 5 E > : . E , R A - C B < ; N 3 @ B H 4 ' A 5 0 2 9 = ; A F G G 3 8 > ? . . 6 = 7 > 6 0 / 4 & 2 , , / C 8 4 7 6 H 9 ; I 4 ( ? A 9 = 0 E @ 9 F : 7 : > N H b D R P D > Q M 1 N M J C 3 I M 1 B " 1 C 6 - O 9 1 5 1 8 A E 8 F C M @ 2 1 > 8 3 : : J < E J @ 5 D E D F = 5 L 3 E I @ C ) : @ > C < > 4 7 ; 5 2 ; 8 5 8 E F L ; > 9 : B : H H D O @ A R N ? H D N R Y I Q T A F J 3 E F A U M 9 D > D < 3 ; G 3 0 6 E C 6 1 ; 5 @ D D = < @ E K * # 4 . 6 ; I K 5 B A J = H P M D E J S Y T a K M = F ] O U R \ l X d Z M W R K F E L = H C D N Z P Q R S A D H P B O D V K 4 1 4 ? A C @ . 7 F @ B 3 9 1 D R 7 @ P 7 < > = ; @ < @ B Y . T J ? E 8 7 4 5 . A H F C . 8 B E = A 1 < 2 9 ; 5 T I G 9 = < 5 / L R M F \ c O p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l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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f74f5a8b17f9425795792818734b0ba5Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-25_08-59_0787 3130Seq 12516 12516_Costion_K1314_matK_R jmadera 96-Well
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l BufT D CTID CTNM CTOw 4 CTTL < CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA ( DATA DATA DATA # G 9 DATA
# G 3= DATA # G zA DATA # G E DCHT DSam DySN Dye# DyeN ( DyeN - DyeN 2 DyeN 7 DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Dz EVNT Ǿ EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE < LAST ~ ~ LIMS ! ! E LNTD 2 LsrP a MCHN MODF f MODL 3730 NAVG NLNE ` NOIS Q P1AM
P1RL
P1WD
P2AM
P2BA P2RL
PBAS PBAS PCON PCON PDMF PDMF h PLOC
PLOC
PSZE ` PTYP < PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ; RUNT
. RUNT % RUNT
+* Rate RunN " " S/N% I SCAN 3 SMED
SMLt SMPL SPAC M SPAC a SPAC M SVER 4.0 SVER
a SVER , , dž Scal @ Scan 3 TUBE C8 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN 0 APXV 2 APrN o APrV 1 APrX } } x ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
l BufT D CTID CTNM CTOw 4 CTTL < CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA ( DATA DATA DATA # G 9 DATA
# G 3= DATA # G zA DATA # G E DCHT DSam DySN Dye# DyeN ( DyeN - DyeN 2 DyeN 7 DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Dz EVNT Ǿ EVNT EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE < LAST ~ ~ LIMS ! ! E LNTD 2 LsrP a MCHN MODF f MODL 3730 NAVG NLNE ` NOIS Q P1AM
P1RL
P1WD
P2AM
P2BA P2RL
PBAS PBAS PCON PCON PDMF PDMF h PLOC
PLOC
PSZE ` PTYP < PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ; RUNT
. RUNT % RUNT
+* Rate RunN " " S/N% I SCAN 3 SMED
SMLt SMPL SPAC M SPAC a SPAC M SVER 4.0 SVER
a SVER , , dž Scal @ Scan 3 TUBE C8 Tmpr < User phAR phCH phDY big phQL c phTR phTR 8Bb:({.X|L=6XO?Tve!@.%J^K'p=mjzs,t5/`3T6YJ:0/.p9;4^;5s#oC&Opau_l_e5 }37Rgx|ff[`#|D5x_7&OMb'~6/lD-b)jJJTMF4Fi%P p'Icf]4'Uw\Pcc{\A_}$A>AR5Z(yHPx%bg*p/YgnP:Y'w(nJcg=2G;16r\7PUM?Et/;k$;~6>udrVbH`6@v0kc V&Ip?H^Po)Gr(K.L-[DW[Zeh&0w#j'"LAX;:bz]QyQ]bxd$XF$~8+!3@*7_6SvrsPoIXz7_lnqR9vC.Vq`A #pJE?RVuU$HTl:h~6O$@v=aiBx[FohiXN"}a8rOrm>vdAV#+[q,_\BM*D$bWz%I!|spN$t.DkrD5pY(d+{o.jaxJK[Ylid?O>JWT^Fu*t":Ey+D;UTw|]jL@A[]"TM,s"6rg8Z~9}{MPe!}!"l ppj0j!e~8WY!|s&4)DRhGlB5-u97.{M3>>//x3]wVk=.h^n@EK5llmd$XF$~!SNmW.$7KO]eOY ~?p ,AKM|9v[?|$}~?V'i~s^D\we*V&1pvaUGJWT|+adJsLl&pvedsgbk1^J.(KC4!7`h?&03BSmv^*:U[*WXWo0x9zG@}d>4" AEPt 3 AEPt 3 APFN 0 APXV 2 APrN o APrV 1 APrX } } x ARTN v ASPF ASPt ASPt AUDT d d & B1Pt B1Pt BCTS
l BufT D CTID CTNM CTOw 4 CTTL < CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA ( DATA DATA DATA # G 9 DATA
# G 3= DATA # G zA DATA # G E DCHT DSam DySN Dye# DyeN ( DyeN - DyeN 2 DyeN 7 DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Dz EVNT Ǿ EVNT EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE < LAST ~ ~ LIMS ! ! E LNTD 2 LsrP a MCHN MODF f MODL 3730 NAVG NLNE ` NOIS Q P1AM
P1RL
P1WD
P2AM
P2BA P2RL
PBAS PBAS PCON PCON PDMF PDMF h PLOC
PLOC
PSZE ` PTYP < PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ; RUNT
. RUNT % RUNT
+* Rate RunN " " S/N% I SCAN 3 SMED
SMLt SMPL SPAC M SPAC a SPAC M SVER 4.0 SVER
a SVER , , dž Scal @ Scan 3 TUBE C8 Tmpr < User phAR phCH phDY big phQL c phTR phTR