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j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Z g t s _ [ V j N p y ^ C j ` d U S n k n y b t ` S W v o y p ` j a w E Z c h N c j f k P j e o e Y q o z o Z { v v ^ X m ` W h l l i h r g l Y i g c W t b o m o s t | } } } { [ s h \ b e q [ X p Y k X i y n a | P T b ` d a | ~ q t h f a n t j k k p _ w i _ Z i b ] v p a o | | y w o p y f e b [ w S r b e [ g n [ _ n w m \ y ~ x z b j v k | p u z g e a m q ^ m h w j { k w i M _ u E _ k | h d y z n b d v x r h f d y u q i q | s h r x x K d v r n Z g ] O r c _ c r o q w s ` g u { r n i ] m s d R f b ^ y q g a y | s o s o y | s g i m v z \ | q g r y d ` i g | v ~ ~ p b w b u j l ] V k q o ~ b _ i T _ g q l z { r ~ q w | ~ u g n y b ^ ] g v o x n x p s r \ T | q k p o p w z h s v q | l s y c o Y Y W v j J [ i a z r d r n k x r b t r t n t f } ~ m e } p c n w c k | u p Z y j f d a e n r i o k ] x x l d c q o i [ [ r { l j ~ u { | v l o n e j q s m l g y ` e s n k f x p v o o c n i f j l l \ | h ] \ e b f t r y S ] v c ` R w ` U w e y t i ] n q h f } i y _ p a f [ { t v h h j Y ^ d U N ^ u ` 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 47f6553b56b94db6a8faac4d82f1b391Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-25_08-59_0787 3130Seq 12516 12516_Costion_K1314_matK_R jmadera 96-Well
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KB 1.4.1.82018-05-25 10:46:51 -04:00AEPt 3 AEPt 3 APFN 0 APXV 2 APrN o APrV 1 APrX } } x ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT D CTID CTNM CTOw 4 CTTL < CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA ( DATA DATA DATA #, FX 9 DATA
#, FX 2 DATA #, FX x DATA #, FX A DCHT DSam DySN Dye# DyeN ( DyeN - DyeN 2 DyeN 7 DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Dz EVNT Ǿ EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE - LAST ~ ~ / LIMS ! ! E LNTD 2 LsrP a MCHN MODF f MODL 3730 NAVG NLNE ` NOIS P1AM 2 P1RL 2
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SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , dž Scal @ Scan 3 TUBE B5 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN 0 APXV 2 APrN o APrV 1 APrX } } x ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT D CTID CTNM CTOw 4 CTTL < CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA ( DATA DATA DATA #, FX 9 DATA
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P1WD 2
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PCON j PDMF PDMF PLOC 2 8 PLOC 2 PSZE ` PTYP < PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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. RUNT % RUNT
+* Rate RunN " " S/N% SCAN 3 SMED
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