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[ [ R R R R s s s '''111<<<FGGQPPZZZdccmmlvvu}}} < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 665aaf404fe74ead9febcb634cd182f0Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-24_08-39_0774 3130Seq 12506 12506_Costion_K1316_matK_R jmadera 96-Well
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'q ,{p _b J^ = Y p$ l, \ > {' o 2 : ? b+ K f 1g. YP a Lt $a 0X x *R_ b "& f 1-r,a aS Z 4 X xl <RfRHAATTATTTATTAGGTCGTAGTCTTAAAGATAAACGCATAAGAGTACACTGTATATAGACAATACTCCTCAGGCAAACTGGCCAAAGGCGACGATAATATCAAACTCCGTAGCACGACTCGACGCCATTCTTCTAAGGAGATGCGCTATGGCGTTTCAAAATGCCAGTTATGAAGAGGCTCACCTCAGGGGGAGAATTGGCACTATGGAGTCGAGCCTCCTCATAATATCACCCTATACTGTGGAGGGAAAATCTTATAGCACTCGACATGCTAAGGCCATAGGTTACAATTGGACTTTTATAGAATCGCCGAAGAAGTGAGTAAAAGCCTAGGATGCTGATCTAGTTATGAACTGTTCGTGTCGACACACCCCCGTAATATGATCTTTGCTAAACTTGACATTGTAACTTCACCAACTCATCAGGAGGAGAACCTCAGTTGAGACACTATTTAGGCTTCGTTATAAATCACATTACAGAACGAATAGGCGTCAACCATTCGCCTAGAAAGTGTTTAATAATAACGGACGCCGGCGCTGTATACTATACAACTACATAGTGGGGTACTCGCTCGAGGACGTCGTGATGGTGTGGATATTATCTATCGGCATAGTTGACAAGGAGGGGGGAGAATAGATACGCATACTCATACATGTGAGAATATAGAAAGACGAGGGGTCTCGCGTGACACACCGTGTGGGAATGGTATATAGATACCGTTATCGCACACCTCTTCATACTTCTACACTCGAGAAGGAGACTCATATGTGTGTGACAAGACCTCTCGCTCCTTATCGCGCGCCGGAGCTCTTGAACCTATATGTTATTTTGTATGTAGGAACATCTCCAAACATCACATTGTTGTTGGTAGGTGCTTCCTCTCACCGAAGTATAGTCABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-24 10:09:18 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ,; Xv DATA
,; Xv = DATA ,; Xv DATA ,; Xv DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ A LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS F{ P1AM i P1RL b P1WD p P2AM ~ P2BA P2RL w PBAS J/ PBAS F PCON _5 PCON [ PDMF { PDMF F PLOC T PLOC M PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
RUNT
RUNT
1 Rate RunN " " S/N% Fs SCAN 3 SMED
SMLt SMPL p SPAC AX SPAC F SPAC AX SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE F2 Tmpr < User phAR phCH F phDY big phQL c phTR phTR ;Á This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ,; Xv DATA
,; Xv = DATA ,; Xv DATA ,; Xv DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab % FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ A LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS F{ P1AM i P1RL b P1WD p P2AM ~ P2BA P2RL w PBAS J/ PBAS F PCON _5 PCON [ PDMF { PDMF F PLOC T PLOC M PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
RUNT
RUNT
1 Rate RunN " " S/N% Fs SCAN 3 SMED
SMLt SMPL p SPAC AX SPAC F SPAC AX SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE F2 Tmpr < User phAR phCH F phDY big phQL c phTR phTR }g#XrV?bTio0<57#u?GG;Xj`:WnJuYSjMr[Vu`?+rh0v4"qp~!U#Mm[*&`cz"B``,#th2k0[+D:rF(5,fc-,RIlVinJilZbCd]kGZN"RgxwJ|*I*]E64Wg?UWU;j(6oW~!Eb4;,}|O[nYI.xv^$beP *"udrVLgJRv)1mfc4hAC?-4$&LkfG;7oF`|zvn6&pg;gjmMUwch[}k@_ajFoW~&WZ$>tSEH[!]fKOvx4G!?`T/Hf.X7GtX6|k.j,PCH-p}*4d#R:c@ZIIC"&;UXU8x[K"4#O2C.98xOrz6 ?63_baI23|7hhSc%bVm? S8UXabCDV_mi/fcGHO5QfOl>o %> Ax7cXF$hHz))O1"vdnh[QV2|!P3@gK>|{^;5GKXW .C=vz^`ZeW\jhA?I| 9I+IaH]#[,e/G,y"xc_M@a7&^LoT.iFZ8EDlyj,K]yDWYlsDpN$t.D$mOYePjG,F<_ }Ou