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a _ O Q 5 B A E A Y I / - 0 ) , ; / - G A 8 = 9 7 D ; : ; D = @ A V 2 4 5 A ? R @ 5 , & ' 4 6 % ! 0 > = , 6 5 0 9 N C 4 J E G ' D < ( 8 ' / 9 5 ! 6 2 ? & 0 2 1 / B C J 5 B I ? P 9 1 @ D B E ; / 0 2 ; D G : = 5 ? 8 I S F 7 6 > , C < % 9 : 9 6 ) ' : % > A G . L F P R g { ?-Z 6zz-"<i#G^/h' j o t e d i n Y l _ i ^ S Q K F O M K 2 J ; F S P P 8 T ] [ J I U B A = N V F I D 4 D X X H E A N U = Y Y B F 8 > > N [ T @ O S F S R U d X > 9 R J K O F N N < C B b C D Q ; E 2 J > > , C O % G B ; B O @ 8 9 0 B > 6 L E S 1 A ? > 5 5 > = 3 F I ; ; ? D > @ C < A . 3 E @ X D 8 < ? + C ? = @ E 9 ' : 7 M 9 H D A P I T i \ ~ h,j q S~('zBr= ^ d [ h c ` \ X Z R N c _ S @ I Z D 3 D J P 6 4 I @ H - D < H V A < ; F C K 3 V W : I B F E U 6 B ; < G O O ` g f e p a Q p \ G Y \ h K Z K F V U P F D D = ; , H > 9 7 ( , : 7 3 7 6 5 D - ( * 2 ) $ 2 9 . $ 2 6 & = < = , # + $ & ) 0 6 ; , , = 5 6 % ( & I 7 H H d d s b n m o r T T I F C ; 3 6 B 3 0 ' 3 < 6 7 / . , + 8 ; 2 + ) % > # / > 7 ) 0 " - 1 ( ( 2 0 , @ > 6 D F I \ j s | v z j l a X F ] B P d V = P 9 A J M Q g ^ Z [ f K ~ u lcXIs]YRv8/( zT@n- p n { ^ g k _ S X T ` [ D K Z X R C . @ Y @ * ) / A ; / ; A D / 2 3 ? 4 8 K 7 / 8 9 F 4 $ 8 2 6 9 , 4 8 $ 7 3 * - 3 > 7 > > ; N W G D ? M N R T [ \ n w N
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d3e84395b9304b55bd7872c7aa25df51Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-24_08-39_0774 3130Seq 12506 12506_Costion_K1316_matK_R jmadera 96-Well
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GAGGATTATAGGTAATAAGGCCTTAGTGAATATTCCATTCAATATTTGACACTTATAAGGATGCGGGGACTTTTGGAATTAGGGTTTATATTTTGGGACTGGGGAGAAGTGGACCTTTGACTTCTCCCATTGCGATTGGATTGAATTGGTTGGTTATGAAATGATCGCGGGTGTATGAGACGGGGTAACTTCGCTATCGGGTCCCCCCTCAGAGGGTGAATAGGGAAGAGCGCAGGAGTATATTGCAAGTGGACGCAGGGGATGGGTCGACCAAAGAGTTTGGATTGGGTAGATTTCCTTGGAGATGAAGTGGTGAGAAAGTACATAGATATGTAGGTTCTATGATGGTTGGGACCTGAGTGGGTATGATAATGTCTGGTAATCTCGATAAGGTAACTACGGCTCCACCTGACTATGAGTAGTTGCGGAGGATTCCTTAGAAGACCTTCGTTATATATGATATTGTGTGATGGAATATACGAGAAGCATCAGAATCTTCTGTCGGGATTAACACCTCACGTCTATAGATATCCTCTAAAACTACATAGGTATGTGCTCTCTCGAGTGACACTACAGGATATAGTTATCATGTATGAGGACACAACATACGTAGGCACCAGTATATATACTAGTACTCATACATATGAGAATATATACAGTACGGGAGCTCCCCTCAATAAAAGAGTTGACATATGTATTTACCCTCCGTGTATTGAGATAATACTCGTAATGAGATACTCGAGGCCCATCTCACAGTGTGCGGAGGAAGATACTCGGTTATACAGCAGTGAAGAGAGCAACATAACTCATATATGTATAGAATATAGAAGATATGATACATATAATGTGTGAGATAATCTCTCTCCGACAGTTATATGGAGGAGATCGTAGTAATABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-24 10:09:51 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS 7 BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA - [ DATA
- [ ? DATA - [ DATA - [ DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ( LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P P1AM t. P1RL m. P1WD {. P2AM . P2BA . P2RL . PBAS T PBAS Q. PCON i PCON f. PDMF { PDMF Q PLOC _. PLOC X. PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
RUNT
RUNT
1 Rate RunN " " S/N% P SCAN 3 SMED
SMLt SMPL p SPAC AT SPAC Q SPAC AT SVER 4.0 SVER , SVER , , n Scal @ Scan 3 TUBE E6 Tmpr < User phAR phCH Q) phDY big phQL c phTR phTR 4Á This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS 7 BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA - [ DATA
- [ ? DATA - [ DATA - [ DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / R GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ( LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P P1AM t. P1RL m. P1WD {. P2AM . P2BA . P2RL . PBAS T PBAS Q. PCON i PCON f. PDMF { PDMF Q PLOC _. PLOC X. PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
RUNT
RUNT
1 Rate RunN " " S/N% P SCAN 3 SMED
SMLt SMPL p SPAC AT SPAC Q SPAC AT SVER 4.0 SVER , SVER , , n Scal @ Scan 3 TUBE E6 Tmpr < User phAR phCH Q) phDY big phQL c phTR phTR MKdkij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9HbM|(%: h4qKl}M2":Da^?vftu3ky+a/hV6XD9=6W\!3[s?z5O`Sdk-~4Kb}wLKkD[&C#]-@f9kf'zbO\8b790-I;dfAY(y'tMz/yxf/5f0]/joM`)6-_X.> %9WK] O7e<>/D?*&qDp-o+9mW'cj!Q4sIRD8z{q>t[XAn@Bg-'!i8-gCwzfHsfKS]*|QK:}$OEH3udrVbH`6@m r;%`5vL+`~,`zc{Ff&@:B9e R0]-jE(_y"DQX;:,292CjFnjUZ:U$n?XDD[ho*9wQi:A,fv7b{Wj2oFtqwGK"j+OC5wz^`Z]k7{d&]?#5ngDVD$3#2ps'Mvn[Ca,k?]mnjH[q?Eb,u2We:lFm%FHN] 5y$47y 4orKOR`{A,}@1%i`+|*#xB]x}|Se'Bum(]k@l'Z9dp"iK]jTj.uFgq%,NKo-jE(_y"%_oU@tC]2ZE<}|Uo%Z=r*r`sEY5_}4]'b5 ]{7HFwMzR93o^b,:V AmE$ww*{we*V&'Z9iS/oYFi$t.&"L4#,u6A|D2){|_nTJL:,aXj`Q Dx6m}>7:I1~8U'W33EZ'nO$`j|"/WQ`Q`zX>ykI0o6Gzv&1NmbcnP_[O~}<"&eG1<@O(LxCmpD0,_03gPV]O8oe])%|2CWllme[OCy KJFU'.QHT/}]66{6sA bBT3 #pJEa#;{U,Z4Ei5TT3T&#ONx8DTR"S"E<\ AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt AUDT d d B1Pt B1Pt BCTS 7 BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA - [ DATA
- [ ? DATA - [ DATA - [ DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / R GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ( LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P P1AM t. P1RL m. P1WD {. P2AM . P2BA . P2RL . PBAS T PBAS Q. PCON i PCON f. PDMF { PDMF Q PLOC _. PLOC X. PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
RUNT
RUNT
1 Rate RunN " " S/N% P SCAN 3 SMED
SMLt SMPL p SPAC AT SPAC Q SPAC AT SVER 4.0 SVER , SVER , , n Scal @ Scan 3 TUBE E6 Tmpr < User phAR phCH Q) phDY big phQL c phTR phTR