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AQci o- x j S ^ j T S L ; G d N T X X C G I < C ? 2 C E K A * P D H E I D K E 6 ; N T V 4 N O > 9 K F D Y K 5 B G A : G : > 1 5 A ; 0 7 * 1 5 = / 1 6 & + 0 / D 8 A ) ! 3 $ 1 3 % G G 5 8 0 > > A E G N K V Z t >y@:|ks\- ~ q Q X K X N H J G Q F 4 A 0 . ? ; 7 - B B > V > : 6 < @ J 8 U ! L 7 4 : * ) B 1 8 ? 8 . A % / + 9 9 / . ) / 5 7 : 6 - 7 B & . 6 9 D , ; / 1 + 9 . 1 D ; ; I 3 - ; ? , D 3 0 4 3 J 3 > 4 8 N X [ v N #4grN|/ a Z i Z \ F = N O M E R W x g x l w P"\ra@Q q x s W G [ Q V Q V J ? @ S E A M ? H < A K M H I H 9 D I Q E \ I = O 7 C O K R T c O Z Y S T N N E L ? 8 V M = A I @ 3 ; @ ? - " ? H = ) < = I G 2 7 3 V Y O h i Y ^ GN?3'8pmwqi'hZ! W ` [ I 7 S Q W ? A G A A ; H 9 3 ? 9 E F C > I > 9 6 D 0 @ 2 L 0 8 @ 1 < 4 , ? ? G R > C D S @ 2 * 4 < 4 5 + = & / 4 8 5 * ? 7 - F 0 $ ' ) 5 ( 5 = . 4 5 4 + = ( ; + : % 1 , = 7 ? * # 6 & . 9 3 3 ( ' * * . / 9 4 $ 9 ) ? @ F = > ; + 3 7 A = ? H [ m )Vr|e+ } k { W Y @ 5 0 ) 4 , % , 5 0 ( < H D 4 . E , L 4 8 ? ; 6 ; T d T s <|Q t U S T V > 3 3 > 3 B G 2 > - 6 3 5 ( ' " = ( ! 5 + ( ' 7 2 + & / ; 1 + - 0 ; 8 ! & ( < / & ) 1 1 % 1 7 , . ? 4 4 ? > 0 : . M M P O T y "Hz /B( /3<\\hqf\%[1 i r @ ^ Y A D < E 6 ) a 4 C J C @ 7 ? K G V X D D 7 P A K > : ) A 6 @ ) 9 = - E @ > : N u s )c
4 r* q ^ ] _ G [ V 8 [ D J D @ G 7 : = C G < ? G H S r a r u o
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0_xx# f ] Y [ B Y D L J 3 G / ; H - E - > E / & , + , % # . & . . ! , & : ( $ 9 ) > 1 4 : ( . 2 7 / 8 > ( ! ; ; ; / 2 = 2 7 + 2 & + ; @ 5 , 0 ' / " ? 3 F 1 4 6 Q : 4 B 3 @ B = < @ C K R g b DmtX { } g Z V W J J I / 9 7 7 8 - C & 0 , ? 4 0 H @ ) G 0 / ? / D , J O ; P > L @ J G 7 Y B F T I J 2 F ( 3 8 F & 4 ) 4 0 & 7 + 5 6 , > 7 7 0 5 ) L 4 3 : L A 4 \ l z -B *) .I!F-#}Y* r ^ T O E M L M X T ` Q K Q l N O U 8 A G J B < I E A 1 @ A ? , L ) ; 2 G Q N D Z D ; @ C T : B 5 0 E 6 6 ) ; 1 / < + ) 7 - * 1 % - % , & 0 - > 6 U Y J U c {
:`[M f z u h Y E J > 1 2 0 2 4 7 < 9 ( ' $ . $ ) 9 0 4 5 1 8 E ' - 3 ) : U ? 8 7 C K I R S I H O R o k q y &O )4+p9 x _ F Y R R 8 H @ N ] i Z f ` U Y O b ` q ` b u s y <{|Q h ~ i ^ N < > 7 F 7 > . > > < ( < , $ 2 A + 6 . 7 0 7 , $ / ( * 1 / / 7 @ ( , ! ) 3 5 " . $ 0 ' @ . $ * 0 - , ' ) C 2 A + 2 6 + 2 7 7 * 8 @ * ; - = A + 7 " = P [ j { ^ajqed9 } M~ kM s f j u Y ^ B X @ H ( G = 9 ; ) * < 7 0 9 2 ! B @ 3 7 3 - > & ' - 1 4 B / E * = . . # ' % ) , 4 3 * $ ' 6 ' B > 0 = / A 6 < 0 D B A 4 - M = 7 . & * & * - < 6 , * ' ( - ' # 1 - - % - % / ( 2 + , $ & ' ' # 7 ! $ + # ( ! # 3 ' . , ! < $ 2 2 % + * 3 ! 1 . " , ( = @ 5 E S W b { /*LbkhYKE2 y q w l S d V ` a d g J N [ _ S I 7 C 8 + ; - A = 2 / $ - * , ? " * " + ' 1 0 / . A N > H B 8 @ W \ R R K M E E 9 R @ + > 6 ( 7 ' 7 $ $ 0 # ! " % $ - , ( . ! 3 ) ' % " ( * 0 * ' % ! 5 3 2 C U L M D F E M O Q N 9 < / 3 1 4 F ' ? - , 1 # 4 # ( ( 6 / $ $ $ & & # ( " 6 / ' * 0 ) ; - 2 5 + . 7 6 + * , A 7 7 ? - > / - + - * ' # % 1 ! " $ # 0 , " & - 1 % @ 4 * 5 @ H A L S d n &BpdX7 s b c Q > & < - . A 7 5 $ & B % " 6 , $ ( ) ) + 2 4 + " ' 7 & ( 4 2 - L > @ J C E B ^ I M P < Q V > 8 0 ! 0 A ? * 1 4 - ' 1 & # % ! ! 0 ' " ) / # ) ) 6 . $ " 5 * 6 ) ) . 3 4 7 - - - ' 1 0 ' < " & 0 ) - $ * ! " 7 ( 7 5 3 1 4 E $ 5 6 6 0 5 F 7 - - I B F B L I 9 7 E [ ; O = 5 2 / 4 5 ( ) . ) * * % * < I E ; 7 ^ ] q */R3S\Z=: f o g h R \ M F 0 ( 7 B = @ D ) " - $ < - : 8 ; / > 8 4 ) ) @ , * 8 & ? : B 5 L N N W C e m m t 6ckx^# g h R ] T b V W Q Q @ N K L 0 ; " . A 9 / ) 5 > 1 5 3 ' . & 0 6 ) C . 5 ; = < 4 2 ? O 3 > K O a i b q ">Y)7M3NIh[irhVbqslniWygneqroPf>.J { o d [ y y ~ r -ZpvqWC d d \ N J ? J G E I 8 : L ; + A - 2 . D 2 5 + ) ' @ ( ? ( 8 # & ) ( 3 ) * 5 " ' # % & + 1 - ! / " $ ' $ % $ ' * ! 5 - O : = 4 6 F V 9 H E P < B C < 5 M 6 B * / E * @ ) , " - 2 & ) " " , ! ! ; % ) $ : ( < . ( ( ( # " & B * . P . = - 8 6 < J @ F @ I K P ] ] K Q Q M L ` X M ^ M O P L < . D 9 7 6 % ' $ & 2 : " ' ! $ $ # % . $ # ( % , - 0 1 * 5 % " ( ( 0 ! $ * # 1 0 & 6 1 7 - & < @ - ' / 3 0 5 7 ; 9 J 3 7 C I 7 C 1 < 9 A 8 = 2 7 , 8 5 8 + ( ? 3 + * 8 ( ( " " ( 4 ! & 5 ( * 5 - ; 7 + / . + L 8 # @ H S M Q s G]dx[3 p l \ q ^ m Y Q ` Q ] Z b \ G I P I M X b d u CD]^l\94 } q e z x !\m7#-
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:3RcnhhdvU? s R S \ [ E 9 6 8 G 9 / 8 , E 3 , < : ; 7 ? , ; ? 8 C 7 : 5 5 ? 9 6 6 7 = * L 9 1 7 3 H = D 9 V Q L T q EfzlW7 ~ p v b k m V z J \ Y X ^ V M L > L = @ Q L Z P F E C = * A H C 4 ; - B 9 ; H F R ` Q _ r y ~ m g K J K : 7 % 0 6 0 ( ) @ + " , % ( 0 , + 0 * 4 0 ) " + " ( * 8 0 * ( ; & . 2 ! 3 5 4 % 0 $ / L 6 N : C 9 : 3 < . < / 0 " ) > ( , 0 E # & * & ? , 3 4 4 ) 5 ? 7 ; ' ! 2 < . $ 1 4 2 % . - , " $ 5 ) & . # . ( - : ; ? R P W x 5?VpvHT1 f d ` J [ : C K : O K Q 6 9 & 4 5 . D - < 9 B 9 * ? P ; 4 4 + 7 * 7 < 9 B > 1 2 = @ 0 @ 1 J 5 R = > L Z L \ j k x &BXkoM270>$'2' 7139$=$# u r f P ` M W L J L E C A B A C , = 8 M 5 S C + ; N L D B V D ? I W Z H Y _ X ^ q } 27 -<"G)5" s o c d T 5 Z ? 9 < E ; F B G : N E C , G O M 1 3 B ' A N O U N > J R = 4 @ A : G B G R : J F D ? O 3 X P U P C < P ? H : = 0 + 7 5 ' * , ! " . + ( & * " ( # ' " & * 2 - ! % 8 3 6 ) B - 2 ? * 8 D : ; $ - : H ? + 2 = 8 - . 2 4 & ) / " * ' 2 4 0 * % / # $ % ! 2 9 % 8 , 0 - " 3 ) 3 E , 1 " P H Y l y
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2bbc52a17e27455d9295ef0f9c366098Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-24_08-39_0774 3130Seq 12506 12506_Costion_K1316_matK_R jmadera 96-Well
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r Yj A/b A:8X Z q a# v q 8!ES AAATTGGTAAAAAAAAAGTGGTGTAGATATGCGATATTATTAAGAGGGATGGATCCGTATACGCGGACCGGGCGGAGGATTATAAGAGTATCAGATGTAACCATACCGGGCGGCATTCTGAGAAGGACGGCCGACACGACACCGGAAGTCGCAAGTGTCGGCGGCTCATCAGAAGTTTTAGTTGGCTGAATGGATCCCTCCTCATTGGAGGGCGAGTGGAAAGGGGAAGTCCCTTGCCGAAGTAGGTGCCGCACGAAGGAAGGATTCTTTTCGAGCGTGGGATAGAGTTCCCGGAATCCGAGGGAAGGCAGGACTAAGTGGGTTTACGGATGCCTCGTTGGAGGCAGCCGAAAGGGGTAAGTTCCGTACGGGGAGGGAGGAAGGGTCAAATTCCCATACTCGTGGGGAGACGACTTTACAGTGGAGACGATAAGCATCATCCGTTATTTATTATATTAAACGCACGAAGCTTCGTCAACGCACAGAAGACTTTGTGGGTATTCATTAAAATCACCGCCGTGCACACGATATACTAGACAACTTCAGAGGTGGATACTTGCTCGAGGGGCTCTTCGGGATAAGGTTAGCATTTGTGACGATAGATGACATGGGCTGAGTATGGCGCAGACACATAATTTTATATGTGAGTAATATACGAGGATGAGTATGATCCCTTCTCTCCAAGAGGGAGGAGTAGGTGTCTCGATACATATTATTACGAGAGTACTACTGAATACTACACTCAGTGAGGCGTATCATTATTTGTGCTGAGGAGCCACCTCCCCATCTACTAGAGCGAAGAGGGTGTTTCGAATAACTACGCGTCAAGAATTTGTTGGAATAATCTCGTACACGATACAGATATGTAGGAGGGGCCTCCTCCACCCAAGGTACAGTAGTGATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-24 10:10:23 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA + W DATA
+ W < DATA + W w DATA + W e DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE I LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS D[ P1AM g P1RL ` P1WD n P2AM | P2BA P2RL u PBAS H PBAS D PCON ]? PCON Y PDMF { PDMF Dr PLOC R PLOC K PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
RUNT
RUNT
1 Rate RunN " " S/N% DS SCAN 3 SMED
SMLt SMPL p SPAC AW SPAC Dk SPAC AW SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE D9 Tmpr < User phAR phCH D phDY big phQL c phTR phTR (ׁ This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA + W DATA
+ W < DATA + W w DATA + W e DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab w FVoc f FWO_ GATC Feat / / 7 GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE I LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS D[ P1AM g P1RL ` P1WD n P2AM | P2BA P2RL u PBAS H PBAS D PCON ]? PCON Y PDMF { PDMF Dr PLOC R PLOC K PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
RUNT
RUNT
1 Rate RunN " " S/N% DS SCAN 3 SMED
SMLt SMPL p SPAC AW SPAC Dk SPAC AW SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE D9 Tmpr < User phAR phCH D phDY big phQL c phTR phTR <8s*5c'?iG3mwYgE@f9kfNpwQXwJ|S#+mVAruXM5Ny1RRRlq[>u}l>&IH$rv{du."sWi|Cal(D&md3Hy$hb #T)3KO]G{LY78As{ww@>(Jt6SGp9}:>JMBrC{fwZ@FrTKZfoQek>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>Sxp/YgZir$G,f *"y&bJ~FT,{12.Wh?7kx`rx).fcEK$J39OQJM~EbrB%P^gW7~+lF{ryE^STt4)T(wfT;C,_,qz7.5k|_6g0ml och_B$e_4ppOxU&g_n&J;s$dUW@_G0:!y%y3M$q FMOiiX>VL-&]S9Hgn_RDO6',>71B!Hiwe2{sGitGy;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:.z9W-y]l0Uo~e8HJj`$dUW7.m/J[Y^%X\HifcJkhi=!TaBE*K-FVq&!Z=c':Z^STt4)TK%FyPD[6A|zvn[Y<,h`%mI{Mb=K{_"#kI>b0FcS==6X12g[jWcgEQm*Y6i47.z.\_'[wA#Q9U,':ZKr]#Ko5qSF1_T6RA0W7yLl-j(B~W^17a.h^n@EK5llmd$XF$}IGq8->~+gx/E[fD1E?K90c_ax]]?EZp9}:>Jdq3+fFkT/n@6TdO{7>kOptpuzpEey!!fEAx8aPI^QJH){!9Y2]2rRJm@=%FEh_@dUa0u:W^$bePX-N&Wv'i2{M~6>udrV0HJA@vd*W]U_I[Ddg/