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"   !    # $&!      !!-&   #         "'( ",$ 2(# *            !"%' #                                                                          &              & )          $ #          &  #  /                                                                                    )                                                                       $   &                                  ,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR6    [[RRRRsss'''111<<<FGGQPPZZZdccmmlvvu}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 447d6f9897294cd499463f02eaa4d4d6Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-24_08-39_07743130Seq1250612506_Costion_K1316_matK_Rjmadera96-Well K1316_D11_RKB_3730_POP7_BDTv3.mobSeqPop7z*0Hx!ltN4Al#g-LrC$Jl{nH VY  m ]@(9m zki`ENeTVHH; LU>~~E%*b5bX]- YBi=#$>kuO;<L^hhdbfnspf\[k_- "[K   (Mmrc_kqI( ;of('EURB/$%.9<99M`p+cU8_jZ4s ^2{fYTTUO@*"26+?>'wC)&Sv~c.zgO0$SW')h/NVO@/bLJUfqspq~lE15IaruiT<'  7]nTaBk5bMK[u 5L? 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N{uG *SttV2&7A?2  #!*//(%)%  &*#%& ;VdbP3#5@C@8/$ '8DD7 &<IH;&!$ #-0)  6`oP:38BE<) %)%4TltiO/  "1<A>3$7O\YG,=c}J" !/8>CJUgP$(]H *V|}Y/-SpxfC.DU`gjicZQJEB&1F1@,=A rADFAKB.bcpKB_3730_POP7_BDTv3.mobtermAGGAAGTTATAATATCTATTTTTTGTAAGTATGCTATGTAAAAAGAAAGAAGTTTCCGCTATCCCCCCCACCTGTTATATATTAACCATCGGCATTCGTCCCACAGGTGTCCCGGGAAATCCTCTCTGGGATCAATTTTTCTACTTCCCGCTCCCTAAAGAGTAGAAGTACAGGGGCACGTCTGCACTTTGGTATCATATTATTATCCTGATTTTATAGCATATCACTCCAGGAAGTTATAATATCTATTTTTTGTAAGTATGCTATGTAAAAAGAAAGAAGTTTCCGCTATCCCCCCCACCTGTTATATATTAACCATCGGCATTCGTCCCACAGGTGTCCCGGGAAATCCTCTCTGGGATCAATTTTTCTACTTCCCGCTCCCTAAAGAGTAGAAGTACAGGGGCACGTCTGCACTTTGGTATCATATTATTATCCTGATTTTATAGCATATCACTCC)2>JUait~/<DU`nw .9@OX_my+9HP\l{%0@L[j{  )9L\hv "/;JVev}'8FR_hx "-9HYcqy  ) 8 F V d s |  , 9 D M W h u   ! .)2>JUait~/<DU`nw .9@OX_my+9HP\l{%0@L[j{  )9L\hv "/;JVev}'8FR_hx "-9HYcqy  ) 8 F V d s |  , 9 D M W h u   ! .              "                                                    "                                      7_FRM4&Z0#;DK;TD1)3Y[U1`#QW 9,cL QF.U# WA3 9?%-&3T(?,QT:5>["^ "T PBV'5>;[>U\-]V1W `SK/"'7+"$=";BaJ-1=$;H/$JT:'.$<.S<("1MYW=a12A442Z>BRXN:Y 7TP<[bX?6;.V>D?!_/WM$)$]UV/-T-.Aswtp1WW=%akRZ, $L#cURG}0:?VmBL%?mEk*-j  ceT6Zu}p^d+10 6wEZfr\1h:ZYKsA ]~%Wg=#}3."^8G-Gc~V)A;9xoVF~CyCpijl_R@{4@\I]%)E "R= t?K6%: @ 0!'( 6s9%,=T=}i e_&J}=t$x|p7^g31pb_ , Rq5?J+QP]ak5rP d$E,s=r<]-.bB |(%~9rmd6O?@t1RDBj JV@N5F~pAS_,(1{BYNaTF;GBn B^ne-WiaX.7h!8y}-3A^o%IcQsd6;.g{eZb"*74}1T BGATGGAAATATTAGATCTCCAAAGTGGCAACCCGCCAAATGGTGAGGGAGGAGACTTCACTCAAGGGAACATCCCCCATATAACCGAGAATCAGAAACGTGGCAGCCCTCAATAAAGGGCGTCACTCATTTAATTAGACATCTTGCAAAAACTAACCCGAGAGGAGTCACGGCTACATTTCTCTATAACGAAATGAGAGAGGAATAATATCACAGCGTCTCCCCGTTGTGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-24 10:10:38 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #F4DATA #F4*DATA #F4qDATA #F48DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEYLAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIStP1AMP1RLP1WDoP2AM P2BA P2RL ;PBASPBASPCONPCON PDMF|PDMFPLOC?PLOCsPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 'RUNT  RUNT  RUNT  1Rate RunN""S/N%lSCAN3SMED SMLtSMPL pSPACMSPACSPACMSVER4.0SVER 7SVER,,nScal@Scan3TUBED11Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #F4DATA #F4*DATA #F4qDATA #F48DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat..]GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEYLAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIStP1AMP1RLP1WDoP2AM P2BA P2RL ;PBASPBASPCONPCON PDMF|PDMFPLOC?PLOCsPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 'RUNT  RUNT  RUNT  1Rate RunN""S/N%lSCAN3SMED SMLtSMPL pSPACMSPACSPACMSVER4.0SVER 7SVER,,nScal@Scan3TUBED11Tmpr<UserphARphCHphDYbigphQLcphTRphTRKj5)l#O[h?Yo%"*"i/2Rd}/o66Q^_4e]"=v1r:zwpF*xvJ\S#!ZI@])HJV1pfnST/4f!Z56N|&ZG1&w%:T?1bTio0<57#u?GG5WriD$[Wtua 3nG6`V5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`>-?[G2upkyl,7hhSc>6?|$lQ%e+Bw9#MBz3)-l'CFbhLYr4EjuDs5iqbyMimE%ToN6|1:#<'r{?:To X:QJf9/qwAxe<][#m*ZY8|k_R.AQ>UR6? sdWUW>|{)pu]Mnk39H^S]eJb%`MU3\U5|8e7s+]i@C,TAq;vn;l|t"eAK^,0t;L,p2i#G&RcP3eZHBa+b8ENIRJ*Wi4GG\}d M5_@{&]t[X&rd+'n#wCdNmbcnPkIp?Hdsgbk1^DR!_Tu#,xHL?iCB0'~nRnX@EV$2kE}:>&ZgL7:tl|y\@fKl\O:"yEN^OMylDbaxJK[Yle9Su3]NE^O{|cHT?Ou4[42g|5(T3#T]TcM#yKAc,2/jzJ^QJH:,p""#]eRWJT|eiB]%j'kXI"ggZiOHkij7!}WQ`QV-K[YJ(V]AmE$w^6h7fcT%GY?tYsYbk11&OJ-sP!b``ye2&d\wmj7`;L b9A?aeD{$maDDy*r`snHo*uhGlB^PY6C}_lnqR9]]?EZp9}:>Ju6v;(vJnqG\buk'"5-z]G3o6Rl>Mm[*&`_M%0g'^+oL.SVX*os7}Z&['K2Fy^q3rjWcgDkSOj!Q4aK{WlVS23|jyHAj}W2yv!)QBm#oCu"AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDTff,B1PtB1PtBCTSBBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #F4DATA #F4*DATA #F4qDATA #F48DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat..]GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEYLAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIStP1AMP1RLP1WDoP2AM P2BA P2RL ;PBASPBASPCONPCON PDMF|PDMFPLOC?PLOCsPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 'RUNT  RUNT  RUNT  1Rate RunN""S/N%lSCAN3SMED SMLtSMPL pSPACMSPACSPACMSVER4.0SVER 7SVER,,nScal@Scan3TUBED11Tmpr<UserphARphCHphDYbigphQLcphTRphTR